bl21 de3 t1r bacteria  (Millipore)


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  • 94

    Structured Review

    Millipore bl21 de3 t1r bacteria
    Bl21 De3 T1r Bacteria, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 t1r bacteria/product/Millipore
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 t1r bacteria - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Filtration:

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: Recombinant Protein Expression and Purification All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Eluted or cleaved proteins were subsequently applied onto ion exchange and/or gel filtration columns for further purification ( ).

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Eluted or cleaved proteins were subsequently applied onto ion exchange and/or gel filtration columns for further purification ( ).

    Expressing:

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: .. Recombinant Protein Expression and Purification All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Eluted or cleaved proteins were subsequently applied onto ion exchange and/or gel filtration columns for further purification ( ).

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: Paragraph title: Recombinant Protein Expression and Purification ... All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins).

    Spectrophotometry:

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: Recombinant Protein Expression and Purification All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Spectra were collected using a model 202SF circular dichroism spectrophotometer at 25C.

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Spectra were collected using a model 202SF circular dichroism spectrophotometer at 25C.

    Purification:

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: .. Recombinant Protein Expression and Purification All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Eluted or cleaved proteins were subsequently applied onto ion exchange and/or gel filtration columns for further purification ( ).

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: .. All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Eluted or cleaved proteins were subsequently applied onto ion exchange and/or gel filtration columns for further purification ( ).

    Spectroscopy:

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: Recombinant Protein Expression and Purification All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. For circular dichroism spectroscopy, purified proteins were analyzed using a 0.1-cm path length quartz cell in 25 mM sodium phosphate (pH 7.2).

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. For circular dichroism spectroscopy, purified proteins were analyzed using a 0.1-cm path length quartz cell in 25 mM sodium phosphate (pH 7.2).

    Recombinant:

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: .. Recombinant Protein Expression and Purification All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Eluted or cleaved proteins were subsequently applied onto ion exchange and/or gel filtration columns for further purification ( ).

    Article Title: Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation
    Article Snippet: .. All recombinant proteins were expressed using BL21(DE3)-T1R bacteria (Sigma-Aldrich) and purified initially using glutathione agarose beads (for GST-fusion proteins) or nickel affinity resin (polyhistidine-tagged proteins). .. Eluted or cleaved proteins were subsequently applied onto ion exchange and/or gel filtration columns for further purification ( ).