bl21 de3 rosetta  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 de3 rosetta
    Bl21 De3 Rosetta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 rosetta/product/Thermo Fisher
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 rosetta - by Bioz Stars, 2020-02
    91/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
    Article Snippet: Purification of proteins and synthesis of peptides for NMR Full-length human Gαi3 (UNIPROT entry P08754) cloned in a pET24d(+) plasmid (a generous gift from Ichio Shimada, University of Tokyo) was used to purify the protein used in NMR experiments . .. For this, protein expression was induced in BL21 Rosetta (Life Technologies) cell cultures with 1 mM IPTG for 16 h at 23 °C in a modified M9 minimal medium containing 1 g l−1 15 N-NH4 Cl, 2 g l−1 2 H-13 C-glucose and 1 g l−1 of 2 H-13 C-15 N Celtone base powder in 2 H2 O with 30 mg l−1 kanamycin and 34 mg l−1 chloramphenicol.

    Filtration:

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: The proteins were further purified by ion exchange chromatography on a HiScreen CaptoQ column (GE Healthcare) and gel filtration on a Superdex 200 or Superdex 75 column (GE Healthcare) in final buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 0.5 mM TCEP, 0.5 mM PMSF. .. All harmonin constructs were expressed in BL21 (DE3) Rosetta (Invitrogen), as described above and purified using Glutathione Sepharose 4B affinity resin followed by size-exclusion chromatography.

    Ion Exchange Chromatography:

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: The proteins were further purified by ion exchange chromatography on a HiScreen CaptoQ column (GE Healthcare) and gel filtration on a Superdex 200 or Superdex 75 column (GE Healthcare) in final buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 0.5 mM TCEP, 0.5 mM PMSF. .. All harmonin constructs were expressed in BL21 (DE3) Rosetta (Invitrogen), as described above and purified using Glutathione Sepharose 4B affinity resin followed by size-exclusion chromatography.

    Size-exclusion Chromatography:

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: .. All harmonin constructs were expressed in BL21 (DE3) Rosetta (Invitrogen), as described above and purified using Glutathione Sepharose 4B affinity resin followed by size-exclusion chromatography. .. Samples submitted to ITC experiments or crystallization trials were digested with Tobacco Etch Virus protease and incubated with Glutathione Sepharose 4B resin.

    Construct:

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: .. All harmonin constructs were expressed in BL21 (DE3) Rosetta (Invitrogen), as described above and purified using Glutathione Sepharose 4B affinity resin followed by size-exclusion chromatography. .. Samples submitted to ITC experiments or crystallization trials were digested with Tobacco Etch Virus protease and incubated with Glutathione Sepharose 4B resin.

    Purification:

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: .. All harmonin constructs were expressed in BL21 (DE3) Rosetta (Invitrogen), as described above and purified using Glutathione Sepharose 4B affinity resin followed by size-exclusion chromatography. .. Samples submitted to ITC experiments or crystallization trials were digested with Tobacco Etch Virus protease and incubated with Glutathione Sepharose 4B resin.

    Article Title: Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
    Article Snippet: Paragraph title: Purification of proteins and synthesis of peptides for NMR ... For this, protein expression was induced in BL21 Rosetta (Life Technologies) cell cultures with 1 mM IPTG for 16 h at 23 °C in a modified M9 minimal medium containing 1 g l−1 15 N-NH4 Cl, 2 g l−1 2 H-13 C-glucose and 1 g l−1 of 2 H-13 C-15 N Celtone base powder in 2 H2 O with 30 mg l−1 kanamycin and 34 mg l−1 chloramphenicol.

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: .. Protein expression and purification All 6xHis fusion proteins were expressed in either BL21-AI, BL21 (DE3) Rosetta (Invitrogen) or BL21-Gold (DE3) (Agilent) Escherichia coli at 20 °C after induction with 0.2 mM IPTG with or without 0.2% L -arabinose, accordingly. .. Selenomethionine derivatives were prepared using B834 (DE3) E. coli (Agilent) and Selenomethionine medium from Molecular Dimensions.

    Incubation:

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: All harmonin constructs were expressed in BL21 (DE3) Rosetta (Invitrogen), as described above and purified using Glutathione Sepharose 4B affinity resin followed by size-exclusion chromatography. .. Samples submitted to ITC experiments or crystallization trials were digested with Tobacco Etch Virus protease and incubated with Glutathione Sepharose 4B resin.

    Nuclear Magnetic Resonance:

    Article Title: Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
    Article Snippet: Paragraph title: Purification of proteins and synthesis of peptides for NMR ... For this, protein expression was induced in BL21 Rosetta (Life Technologies) cell cultures with 1 mM IPTG for 16 h at 23 °C in a modified M9 minimal medium containing 1 g l−1 15 N-NH4 Cl, 2 g l−1 2 H-13 C-glucose and 1 g l−1 of 2 H-13 C-15 N Celtone base powder in 2 H2 O with 30 mg l−1 kanamycin and 34 mg l−1 chloramphenicol.

    Expressing:

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: Paragraph title: Protein expression and purification ... All harmonin constructs were expressed in BL21 (DE3) Rosetta (Invitrogen), as described above and purified using Glutathione Sepharose 4B affinity resin followed by size-exclusion chromatography.

    Article Title: Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
    Article Snippet: .. For this, protein expression was induced in BL21 Rosetta (Life Technologies) cell cultures with 1 mM IPTG for 16 h at 23 °C in a modified M9 minimal medium containing 1 g l−1 15 N-NH4 Cl, 2 g l−1 2 H-13 C-glucose and 1 g l−1 of 2 H-13 C-15 N Celtone base powder in 2 H2 O with 30 mg l−1 kanamycin and 34 mg l−1 chloramphenicol. .. The pelleted cells were lysed by sonication in 50 mM Tris, 200 mM NaCl, 100 mM KCl and 1 mM DTT at pH 8.0, and the supernatant was loaded on a Ni2+ -loaded His-Trap FF column.

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: .. Protein expression and purification All 6xHis fusion proteins were expressed in either BL21-AI, BL21 (DE3) Rosetta (Invitrogen) or BL21-Gold (DE3) (Agilent) Escherichia coli at 20 °C after induction with 0.2 mM IPTG with or without 0.2% L -arabinose, accordingly. .. Selenomethionine derivatives were prepared using B834 (DE3) E. coli (Agilent) and Selenomethionine medium from Molecular Dimensions.

    Modification:

    Article Title: Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
    Article Snippet: .. For this, protein expression was induced in BL21 Rosetta (Life Technologies) cell cultures with 1 mM IPTG for 16 h at 23 °C in a modified M9 minimal medium containing 1 g l−1 15 N-NH4 Cl, 2 g l−1 2 H-13 C-glucose and 1 g l−1 of 2 H-13 C-15 N Celtone base powder in 2 H2 O with 30 mg l−1 kanamycin and 34 mg l−1 chloramphenicol. .. The pelleted cells were lysed by sonication in 50 mM Tris, 200 mM NaCl, 100 mM KCl and 1 mM DTT at pH 8.0, and the supernatant was loaded on a Ni2+ -loaded His-Trap FF column.

    Sonication:

    Article Title: Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
    Article Snippet: For this, protein expression was induced in BL21 Rosetta (Life Technologies) cell cultures with 1 mM IPTG for 16 h at 23 °C in a modified M9 minimal medium containing 1 g l−1 15 N-NH4 Cl, 2 g l−1 2 H-13 C-glucose and 1 g l−1 of 2 H-13 C-15 N Celtone base powder in 2 H2 O with 30 mg l−1 kanamycin and 34 mg l−1 chloramphenicol. .. The pelleted cells were lysed by sonication in 50 mM Tris, 200 mM NaCl, 100 mM KCl and 1 mM DTT at pH 8.0, and the supernatant was loaded on a Ni2+ -loaded His-Trap FF column.

    Crystallization Assay:

    Article Title: Myosin 7 and its adaptors link cadherins to actin
    Article Snippet: All harmonin constructs were expressed in BL21 (DE3) Rosetta (Invitrogen), as described above and purified using Glutathione Sepharose 4B affinity resin followed by size-exclusion chromatography. .. Samples submitted to ITC experiments or crystallization trials were digested with Tobacco Etch Virus protease and incubated with Glutathione Sepharose 4B resin.

    Plasmid Preparation:

    Article Title: Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif
    Article Snippet: Purification of proteins and synthesis of peptides for NMR Full-length human Gαi3 (UNIPROT entry P08754) cloned in a pET24d(+) plasmid (a generous gift from Ichio Shimada, University of Tokyo) was used to purify the protein used in NMR experiments . .. For this, protein expression was induced in BL21 Rosetta (Life Technologies) cell cultures with 1 mM IPTG for 16 h at 23 °C in a modified M9 minimal medium containing 1 g l−1 15 N-NH4 Cl, 2 g l−1 2 H-13 C-glucose and 1 g l−1 of 2 H-13 C-15 N Celtone base powder in 2 H2 O with 30 mg l−1 kanamycin and 34 mg l−1 chloramphenicol.

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    Thermo Fisher bl21
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    Thermo Fisher escherichia coli bl21 rosetta de3 competent cells
    Escherichia Coli Bl21 Rosetta De3 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 rosetta de3 competent cells/product/Thermo Fisher
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