bl21 de3 ril  (Millipore)


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  • 92

    Structured Review

    Millipore bl21 de3 ril
    Bl21 De3 Ril, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 ril/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 ril - by Bioz Stars, 2020-04
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A unique pH-dependent recognition of methylated histone H3K4 by PPS and DIDO
    Article Snippet: The Drosophila PPS PHD finger constructs, aa 904–965, aa 908–965, and aa 910–964, were cloned from full-length PPS in pCaSpeR4 (a kind gift from Helen Salz) into a pDEST15 using Gateway cloning technology. .. Proteins were expressed in Rosetta2 (DE3) pLysS or BL21 (DE3) RIL in either Luria Broth or M19 minimal media supplemented with 50 μM ZnCl2 and 15 NH4 Cl (Sigma-Aldrich).

    Centrifugation:

    Article Title: A unique pH-dependent recognition of methylated histone H3K4 by PPS and DIDO
    Article Snippet: Proteins were expressed in Rosetta2 (DE3) pLysS or BL21 (DE3) RIL in either Luria Broth or M19 minimal media supplemented with 50 μM ZnCl2 and 15 NH4 Cl (Sigma-Aldrich). .. Cells were induced with 0.2–0.5 mM IPTG (Gold biotechnology), grown for 16 hours at 16–18°C, and harvested by centrifugation.

    Positron Emission Tomography:

    Article Title: Identification and characterization of trans-3-hydroxy-l-proline dehydratase and ?1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria
    Article Snippet: .. The five former and pET/C14orf149 were transformed into E. coli strains BL21(DE3) and BL21(DE3)-RIL (Novagen), respectively. .. 4.6 Expression and purification of His6 -tagged recombinant proteins E. coli harboring the expression plasmid for His6 -tagged proteins was grown at 37 °C to a turbidity of 0.6 at 600 nm in Super broth medium (pH 7.0, 12 g tryptone, 24 g yeast extract, 5 mL glycerol, 3.81 g KH2 PO4 , and 12.5 g K2 HPO4 per liter) containing 50 mg/liter ampicillin.

    Mutagenesis:

    Article Title: A unique pH-dependent recognition of methylated histone H3K4 by PPS and DIDO
    Article Snippet: Point mutants were generated using site-directed mutagenesis. .. Proteins were expressed in Rosetta2 (DE3) pLysS or BL21 (DE3) RIL in either Luria Broth or M19 minimal media supplemented with 50 μM ZnCl2 and 15 NH4 Cl (Sigma-Aldrich).

    Construct:

    Article Title: A unique pH-dependent recognition of methylated histone H3K4 by PPS and DIDO
    Article Snippet: The Drosophila PPS PHD finger constructs, aa 904–965, aa 908–965, and aa 910–964, were cloned from full-length PPS in pCaSpeR4 (a kind gift from Helen Salz) into a pDEST15 using Gateway cloning technology. .. Proteins were expressed in Rosetta2 (DE3) pLysS or BL21 (DE3) RIL in either Luria Broth or M19 minimal media supplemented with 50 μM ZnCl2 and 15 NH4 Cl (Sigma-Aldrich).

    Generated:

    Article Title: A unique pH-dependent recognition of methylated histone H3K4 by PPS and DIDO
    Article Snippet: Point mutants were generated using site-directed mutagenesis. .. Proteins were expressed in Rosetta2 (DE3) pLysS or BL21 (DE3) RIL in either Luria Broth or M19 minimal media supplemented with 50 μM ZnCl2 and 15 NH4 Cl (Sigma-Aldrich).

    Amplification:

    Article Title: Identification and characterization of trans-3-hydroxy-l-proline dehydratase and ?1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria
    Article Snippet: Each amplified DNA fragment was introduced into BamHI-HindIII sites (for PaLhpD , AbLhpH , AbLhpI and CpLhpI genes) or BamHI-PstI (for C14orf149 and CpLhpH genes) in pETDuet-1 (Novagen), a plasmid vector for conferring N-terminal His6 -tag on expressed proteins, to obtain pET/PaLhpD, pET/AbLhpH, pET/AbLhpI, pET/CpLhpH, pET/CpLhpI, and pET/C14orf149. .. The five former and pET/C14orf149 were transformed into E. coli strains BL21(DE3) and BL21(DE3)-RIL (Novagen), respectively.

    Expressing:

    Article Title: Identification and characterization of trans-3-hydroxy-l-proline dehydratase and ?1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria
    Article Snippet: Paragraph title: Plasmid construction for expression of recombinant proteins ... The five former and pET/C14orf149 were transformed into E. coli strains BL21(DE3) and BL21(DE3)-RIL (Novagen), respectively.

    Polymerase Chain Reaction:

    Article Title: Identification and characterization of trans-3-hydroxy-l-proline dehydratase and ?1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria
    Article Snippet: AbLhpH and AbLhpI genes were amplified by PCR using primers designed from putative proline racemase (Bcep18194_B1660) and OCD genes (Bcep18194_B1663) from B. lata and genome DNA of A. brasilense as a template, and the amplified products were sequenced. .. The five former and pET/C14orf149 were transformed into E. coli strains BL21(DE3) and BL21(DE3)-RIL (Novagen), respectively.

    Transformation Assay:

    Article Title: Identification and characterization of trans-3-hydroxy-l-proline dehydratase and ?1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria
    Article Snippet: .. The five former and pET/C14orf149 were transformed into E. coli strains BL21(DE3) and BL21(DE3)-RIL (Novagen), respectively. .. 4.6 Expression and purification of His6 -tagged recombinant proteins E. coli harboring the expression plasmid for His6 -tagged proteins was grown at 37 °C to a turbidity of 0.6 at 600 nm in Super broth medium (pH 7.0, 12 g tryptone, 24 g yeast extract, 5 mL glycerol, 3.81 g KH2 PO4 , and 12.5 g K2 HPO4 per liter) containing 50 mg/liter ampicillin.

    Recombinant:

    Article Title: Identification and characterization of trans-3-hydroxy-l-proline dehydratase and ?1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria
    Article Snippet: Paragraph title: Plasmid construction for expression of recombinant proteins ... The five former and pET/C14orf149 were transformed into E. coli strains BL21(DE3) and BL21(DE3)-RIL (Novagen), respectively.

    Plasmid Preparation:

    Article Title: Identification and characterization of trans-3-hydroxy-l-proline dehydratase and ?1-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-l-proline metabolism of bacteria
    Article Snippet: Paragraph title: Plasmid construction for expression of recombinant proteins ... The five former and pET/C14orf149 were transformed into E. coli strains BL21(DE3) and BL21(DE3)-RIL (Novagen), respectively.

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  • 99
    Millipore escherichia coli bl21 ril codon plus cells
    Escherichia Coli Bl21 Ril Codon Plus Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 ril codon plus cells/product/Millipore
    Average 99 stars, based on 1 article reviews
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    escherichia coli bl21 ril codon plus cells - by Bioz Stars, 2020-04
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    Millipore bl21 codonplus de3 ril
    Bl21 Codonplus De3 Ril, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 codonplus de3 ril/product/Millipore
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bl21 codonplus de3 ril - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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