bl21 de3 plyss  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 de3 plyss
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 De3 Plyss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 plyss/product/Thermo Fisher
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 plyss - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "PROTECTIVE EFFECT OF ScFv-DAF FUSION PROTEIN ON THE COMPLEMENT ATTACK TO ACETYLCHOLINE RECEPTOR: A POSSIBLE OPTION FOR TREATMENT OF MYASTHENIA GRAVIS"

    Article Title: PROTECTIVE EFFECT OF ScFv-DAF FUSION PROTEIN ON THE COMPLEMENT ATTACK TO ACETYLCHOLINE RECEPTOR: A POSSIBLE OPTION FOR TREATMENT OF MYASTHENIA GRAVIS

    Journal: Muscle & nerve

    doi: 10.1002/mus.23247

    Expression and purification of fusion protein. 1—pET16b–scFv-DAF/BL21 (DE3) plyss before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Figure Legend Snippet: Expression and purification of fusion protein. 1—pET16b–scFv-DAF/BL21 (DE3) plyss before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.

    Techniques Used: Expressing, Purification

    Related Articles

    Clone Assay:

    Article Title: Association and Evidence for Linked Recognition of Type IV Secretion System Proteins VirB9-1, VirB9-2, and VirB10 in Anaplasma marginale
    Article Snippet: .. Genes encoding VirB2 (AM030), VirB4-1 (AM814), VirB4-2 (AM1053), the first fragment of VirB6-1 (AM813 F1), VirB8-2 (AM1316), VirB11 (AM1313), and VirD4 (AM1312) were cloned into the pEXP1-DEST vector (Invitrogen), with N-terminal 6×His and C-terminal FLAG tags, and expressed in BL-21 DE3 pLysS One Shot E. coli (Invitrogen). .. Plasmid DNA was extracted from E. coli and sequencing was performed using the BigDye kit and ABI Prism automated sequence (Applied Biosystems) as described previously ( ).

    Affinity Chromatography:

    Article Title: The matrix domain contributes to the nucleic acid chaperone activity of HIV-2 Gag
    Article Snippet: .. The glutathione S-transferase (GST) fusion HIV-2 NC, MA, and GagΔp6 and HIV-1 MA recombinant proteins were expressed in One Shot® BL21(DE3)pLysS E. coli (Invitrogen) and purified by affinity chromatography on Glutathione Sepharose (GE Healthcare) as described in the Additional file . ..

    Purification:

    Article Title: Genetic requirements for replication initiation of the staphylococcal multiresistance plasmid pSK41.
    Article Snippet: .. Replication of staphylococcal multiresistance plasmid pSK41 is initiated by binding of the replication initiator protein (Rep) to the Rep boxes, a series of four direct repeats located centrally within the rep gene. .. Replication of staphylococcal multiresistance plasmid pSK41 is initiated by binding of the replication initiator protein (Rep) to the Rep boxes, a series of four direct repeats located centrally within the rep gene.

    Article Title: The matrix domain contributes to the nucleic acid chaperone activity of HIV-2 Gag
    Article Snippet: .. The glutathione S-transferase (GST) fusion HIV-2 NC, MA, and GagΔp6 and HIV-1 MA recombinant proteins were expressed in One Shot® BL21(DE3)pLysS E. coli (Invitrogen) and purified by affinity chromatography on Glutathione Sepharose (GE Healthcare) as described in the Additional file . ..

    other:

    Article Title: Structural basis for tuning activity and membrane specificity of bacterial cytolysins
    Article Snippet: Therefore, these two ILY variants were expressed in E. coli BL21 pLysS and induced with 50 µg/ml IPTG at 18°C for 1 hour.

    Article Title: α-Conotoxin as Potential to α7-nAChR Recombinant Expressed in Escherichia coli.
    Article Snippet: Drugs 2020, 18, x 4 of 15 into BL21(DE3) pLysS of Escherichia coli to express fusion protein of TrxA-His6-ArIB and TrxA-His6- ArIB (V11L, V16A) under IPTG induction.

    Expressing:

    Article Title: Genetic requirements for replication initiation of the staphylococcal multiresistance plasmid pSK41.
    Article Snippet: .. Replication of staphylococcal multiresistance plasmid pSK41 is initiated by binding of the replication initiator protein (Rep) to the Rep boxes, a series of four direct repeats located centrally within the rep gene. .. Replication of staphylococcal multiresistance plasmid pSK41 is initiated by binding of the replication initiator protein (Rep) to the Rep boxes, a series of four direct repeats located centrally within the rep gene.

    Article Title: Fast purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells
    Article Snippet: .. Expression of recombinant AβM variants in E. coli The plasmid pET3a containing human AβM42 and AβMC40 cDNA was transformed into Escherichia coli (E. coli ) One Shot® BL21 (DE3) pLysS (Thermo Fisher Scientific, USA). ..

    Article Title: Protein prenylation and Hsp40 in thermotolerance of Plasmodium falciparum malaria parasites
    Article Snippet: .. The expression plasmid was transformed into One Shot BL21(DE3)pLysS E. coli cells (Thermo Fisher Scientific). .. Overnight starter cultures were diluted 1:1000 and grown to an optical density (O.D.) of ∼0.6 where 1mM IPTG was added for 16 hours at 16°C.

    Modification:

    Article Title: Genetic requirements for replication initiation of the staphylococcal multiresistance plasmid pSK41.
    Article Snippet: .. Replication of staphylococcal multiresistance plasmid pSK41 is initiated by binding of the replication initiator protein (Rep) to the Rep boxes, a series of four direct repeats located centrally within the rep gene. .. Replication of staphylococcal multiresistance plasmid pSK41 is initiated by binding of the replication initiator protein (Rep) to the Rep boxes, a series of four direct repeats located centrally within the rep gene.

    Transformation Assay:

    Article Title: Genetic requirements for replication initiation of the staphylococcal multiresistance plasmid pSK41.
    Article Snippet: .. Replication of staphylococcal multiresistance plasmid pSK41 is initiated by binding of the replication initiator protein (Rep) to the Rep boxes, a series of four direct repeats located centrally within the rep gene. .. Replication of staphylococcal multiresistance plasmid pSK41 is initiated by binding of the replication initiator protein (Rep) to the Rep boxes, a series of four direct repeats located centrally within the rep gene.

    Article Title: Fast purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells
    Article Snippet: .. Expression of recombinant AβM variants in E. coli The plasmid pET3a containing human AβM42 and AβMC40 cDNA was transformed into Escherichia coli (E. coli ) One Shot® BL21 (DE3) pLysS (Thermo Fisher Scientific, USA). ..

    Article Title: Protein prenylation and Hsp40 in thermotolerance of Plasmodium falciparum malaria parasites
    Article Snippet: .. The expression plasmid was transformed into One Shot BL21(DE3)pLysS E. coli cells (Thermo Fisher Scientific). .. Overnight starter cultures were diluted 1:1000 and grown to an optical density (O.D.) of ∼0.6 where 1mM IPTG was added for 16 hours at 16°C.

    Recombinant:

    Article Title: The matrix domain contributes to the nucleic acid chaperone activity of HIV-2 Gag
    Article Snippet: .. The glutathione S-transferase (GST) fusion HIV-2 NC, MA, and GagΔp6 and HIV-1 MA recombinant proteins were expressed in One Shot® BL21(DE3)pLysS E. coli (Invitrogen) and purified by affinity chromatography on Glutathione Sepharose (GE Healthcare) as described in the Additional file . ..

    Article Title: Fast purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells
    Article Snippet: .. Expression of recombinant AβM variants in E. coli The plasmid pET3a containing human AβM42 and AβMC40 cDNA was transformed into Escherichia coli (E. coli ) One Shot® BL21 (DE3) pLysS (Thermo Fisher Scientific, USA). ..

    Plasmid Preparation:

    Article Title: Fast purification of recombinant monomeric amyloid-β from E. coli and amyloid-β-mCherry aggregates from mammalian cells
    Article Snippet: .. Expression of recombinant AβM variants in E. coli The plasmid pET3a containing human AβM42 and AβMC40 cDNA was transformed into Escherichia coli (E. coli ) One Shot® BL21 (DE3) pLysS (Thermo Fisher Scientific, USA). ..

    Article Title: Characterization of Nora Virus Structural Proteins via Western Blot Analysis
    Article Snippet: .. Plasmid DNA was used to transform One Shot® BL21 (DE3) pLysS Chemically Competent E. coli cells (Invitrogen). .. These cells were subsequently used for expression of the Nora virus genes.

    Article Title: Protein prenylation and Hsp40 in thermotolerance of Plasmodium falciparum malaria parasites
    Article Snippet: .. The expression plasmid was transformed into One Shot BL21(DE3)pLysS E. coli cells (Thermo Fisher Scientific). .. Overnight starter cultures were diluted 1:1000 and grown to an optical density (O.D.) of ∼0.6 where 1mM IPTG was added for 16 hours at 16°C.

    Article Title: Association and Evidence for Linked Recognition of Type IV Secretion System Proteins VirB9-1, VirB9-2, and VirB10 in Anaplasma marginale
    Article Snippet: .. Genes encoding VirB2 (AM030), VirB4-1 (AM814), VirB4-2 (AM1053), the first fragment of VirB6-1 (AM813 F1), VirB8-2 (AM1316), VirB11 (AM1313), and VirD4 (AM1312) were cloned into the pEXP1-DEST vector (Invitrogen), with N-terminal 6×His and C-terminal FLAG tags, and expressed in BL-21 DE3 pLysS One Shot E. coli (Invitrogen). .. Plasmid DNA was extracted from E. coli and sequencing was performed using the BigDye kit and ABI Prism automated sequence (Applied Biosystems) as described previously ( ).

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