bl21 de3 plsys  (Millipore)


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  • 87

    Structured Review

    Millipore bl21 de3 plsys
    Bl21 De3 Plsys, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 plsys/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 plsys - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Clone Assay:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid. .. The bacteria cells were cultured in LB broth and induced for over-night at 18°C with 100 µM IPTG, and cells were resuspended and lysed by sonication in lysis buffer consisting of 50 mM Tris (pH 7.5), 1 M NaCl and 4 mM β-mercaptoethanol.

    Positron Emission Tomography:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid. .. The bacteria cells were cultured in LB broth and induced for over-night at 18°C with 100 µM IPTG, and cells were resuspended and lysed by sonication in lysis buffer consisting of 50 mM Tris (pH 7.5), 1 M NaCl and 4 mM β-mercaptoethanol.

    Binding Assay:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: The supernatant of cell lysates was loaded onto a column packed with GST binding resin (Novagen) and eluted with the same buffer containing 10 mM reduced glutathione. .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid.

    Cell Culture:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid. .. The bacteria cells were cultured in LB broth and induced for over-night at 18°C with 100 µM IPTG, and cells were resuspended and lysed by sonication in lysis buffer consisting of 50 mM Tris (pH 7.5), 1 M NaCl and 4 mM β-mercaptoethanol.

    Purification:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: Paragraph title: Expression and purification of human RIG-I protein ... To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid.

    Produced:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: Recombinant protein was produced in the Sf9 insect cells based on the Baculovirus expression vector system. .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid.

    Derivative Assay:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid. .. The bacteria cells were cultured in LB broth and induced for over-night at 18°C with 100 µM IPTG, and cells were resuspended and lysed by sonication in lysis buffer consisting of 50 mM Tris (pH 7.5), 1 M NaCl and 4 mM β-mercaptoethanol.

    Expressing:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid. .. The bacteria cells were cultured in LB broth and induced for over-night at 18°C with 100 µM IPTG, and cells were resuspended and lysed by sonication in lysis buffer consisting of 50 mM Tris (pH 7.5), 1 M NaCl and 4 mM β-mercaptoethanol.

    Sonication:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: Harvested cells were resuspended and lysed by sonication in lysis buffer consisting of 50 mM Tris (pH 7.5), 100 mM NaCl and 1 mM dithiothreitol. .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid.

    Lysis:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: Harvested cells were resuspended and lysed by sonication in lysis buffer consisting of 50 mM Tris (pH 7.5), 100 mM NaCl and 1 mM dithiothreitol. .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid.

    Recombinant:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: Recombinant protein was produced in the Sf9 insect cells based on the Baculovirus expression vector system. .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid.

    Plasmid Preparation:

    Article Title: 5?-Triphosphate-RNA-independent activation of RIG-I via RNA aptamer with enhanced antiviral activity
    Article Snippet: .. To purify Flag-RIG-I, Flag-Helicase+RD (218–925 amino acids) and Flag-CARD (1–217 amino acids) proteins from the BL21(DE3)-pLsys (Novagen) bacteria, human RIG-I gene was sub cloned into an expression vector derived from the pProEX HTa (Invitrogen) or pET-22b CPD plasmid. .. The bacteria cells were cultured in LB broth and induced for over-night at 18°C with 100 µM IPTG, and cells were resuspended and lysed by sonication in lysis buffer consisting of 50 mM Tris (pH 7.5), 1 M NaCl and 4 mM β-mercaptoethanol.