bl21 de3 competent cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 de3 competent cells
    Bl21 De3 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 competent cells/product/Thermo Fisher
    Average 89 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 competent cells - by Bioz Stars, 2020-02
    89/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Control of Angiogenesis by AIBP-mediated Cholesterol Efflux
    Article Snippet: .. For expression of AIBP proteins, the genes were cloned in frame into pHUE vector (kindly provided by Tracy Handel), expressed in BL21 DE3 competent cells (Invitrogen) and purified with a Ni-NTA agarose resin column (Qiagen). .. Deubiquitinase (DUB) expressed in pHUE was used as a negative control in experiments with recombinant AIBP.

    Centrifugation:

    Article Title: Real-Time Imaging of Rab5 Activity Using a Pre-Quenched Biosensor
    Article Snippet: The pGEX-4T-2-R5BD-BMS and the non-binding mutant (pGEX-4T-2-R5BD(V822D)-BMS) plasmids were transformed into BL21 (DE3) competent cells (Invitrogen) and the recombinant peptides were expressed by induction of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG). .. Assembled RAFBs were cleaved by thrombin from the GST domain and recovered by centrifugation.

    Positron Emission Tomography:

    Article Title: Dihydrophenylalanine: A prephenate-derived Photorhabdus luminescens antibiotic and intermediate in dihydrostilbene biosynthesis
    Article Snippet: E. coli TOP10 and BL21(DE3) competent cells were purchased from Invitrogen. .. Plasmid pET-28b was purchased from Novagen.

    Article Title: The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI
    Article Snippet: E. coli DH5α and BL21(DE3) competent cells were purchased from Invitrogen (Carlsbad, CA). .. The pET-28a Escherichia coli expression vector was purchased from Novagen (Madison, WI).

    Polymerase Chain Reaction:

    Article Title: Parkinson-Related LRRK2 Mutation R1628P Enables Cdk5 Phosphorylation of LRRK2 and Upregulates Its Kinase Activity
    Article Snippet: GST-tagged LRRK2-COR (COR domain, amino acids 1535~1878) was obtained by PCR using FL-HA-LRRK2-WT as template and subcloned to pGEX-2T1 vector (Addgene). .. Recombinant GST-tagged LRRK2-COR protein were induced and purified from BL21 (DE3) competent cells (Invitrogen).

    Article Title: Mutagenesis of the regulatory domain of phenylalanine hydroxylase
    Article Snippet: DNA primers for PCR and sequencing were purchased from Lofstrand Laboratories (Gaithersburg, MD). .. Escherichia coli strains DH5α and BL21(DE3) competent cells were purchased from Life Technologies (Rockville, MD) and Novagen, respectively.

    Mutagenesis:

    Article Title: Real-Time Imaging of Rab5 Activity Using a Pre-Quenched Biosensor
    Article Snippet: .. The pGEX-4T-2-R5BD-BMS and the non-binding mutant (pGEX-4T-2-R5BD(V822D)-BMS) plasmids were transformed into BL21 (DE3) competent cells (Invitrogen) and the recombinant peptides were expressed by induction of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG). .. GST-R5BD fusion proteins were pulled down by glutathione-coated Sepharose 4B beads (GE Healthcare), and conjugated with biotin-Lys(FITC)-Cys(S-S-Dabcyl) through streptavidin.

    Article Title: Parkinson-Related LRRK2 Mutation R1628P Enables Cdk5 Phosphorylation of LRRK2 and Upregulates Its Kinase Activity
    Article Snippet: HA-tagged and GFP-tagged LRRK2 with R1628P, S1627A:R1628P, S1627D, and S1627D:R1628P mutations were performed using QuikChange site-directed II mutagenesis kit (Stratagene), and the point mutations were confirmed by sequencing. .. Recombinant GST-tagged LRRK2-COR protein were induced and purified from BL21 (DE3) competent cells (Invitrogen).

    Article Title: Phosphorylation of ATM by Cdk5 mediates DNA damage signaling and regulates neuronal death
    Article Snippet: Serine 794 and 1981 mutation to alanine (SA) or glutamine (SD) was performed using QuikChange site-directed II mutagenesis kit (Stratagene). .. Recombinant GST-fusion proteins were induced and purified from BL21 (DE3) competent cells (Invitrogen).

    Negative Control:

    Article Title: Control of Angiogenesis by AIBP-mediated Cholesterol Efflux
    Article Snippet: For expression of AIBP proteins, the genes were cloned in frame into pHUE vector (kindly provided by Tracy Handel), expressed in BL21 DE3 competent cells (Invitrogen) and purified with a Ni-NTA agarose resin column (Qiagen). .. Deubiquitinase (DUB) expressed in pHUE was used as a negative control in experiments with recombinant AIBP.

    Construct:

    Article Title: Parkinson-Related LRRK2 Mutation R1628P Enables Cdk5 Phosphorylation of LRRK2 and Upregulates Its Kinase Activity
    Article Snippet: Paragraph title: Constructs and recombinant proteins ... Recombinant GST-tagged LRRK2-COR protein were induced and purified from BL21 (DE3) competent cells (Invitrogen).

    Article Title: THE ROLE OF MAP1A LIGHT CHAIN 2 IN SYNAPTIC SURFACE RETENTION OF CAV2.2 CHANNELS IN HIPPOCAMPAL NEURONS
    Article Snippet: Paragraph title: DNA constructs ... His- and GST-fusion proteins were prepared as bacterial lysates using BL21 (DE3) competent cells (One Shot, Invitrogen) and purified as previously described ( ; ).

    Article Title: Mutagenesis of the regulatory domain of phenylalanine hydroxylase
    Article Snippet: Plasmids pLNC209 ( ) and pLNCB111 ( ) expressing rat and human PAH, respectively, were constructed in this laboratory. .. Escherichia coli strains DH5α and BL21(DE3) competent cells were purchased from Life Technologies (Rockville, MD) and Novagen, respectively.

    Article Title: Phosphorylation of ATM by Cdk5 mediates DNA damage signaling and regulates neuronal death
    Article Snippet: Paragraph title: Constructs and recombinant proteins ... Recombinant GST-fusion proteins were induced and purified from BL21 (DE3) competent cells (Invitrogen).

    Purification:

    Article Title: Parkinson-Related LRRK2 Mutation R1628P Enables Cdk5 Phosphorylation of LRRK2 and Upregulates Its Kinase Activity
    Article Snippet: .. Recombinant GST-tagged LRRK2-COR protein were induced and purified from BL21 (DE3) competent cells (Invitrogen). .. Animals Cdk5 conditional knockout (ko) mice in the forebrain were generated by the crossbreed between B6.129S4(Cg)-Cdk5tm1.1Lht/J (Cdk5-loxP) and CamKIIα-Cre mice.

    Article Title: THE ROLE OF MAP1A LIGHT CHAIN 2 IN SYNAPTIC SURFACE RETENTION OF CAV2.2 CHANNELS IN HIPPOCAMPAL NEURONS
    Article Snippet: .. His- and GST-fusion proteins were prepared as bacterial lysates using BL21 (DE3) competent cells (One Shot, Invitrogen) and purified as previously described ( ; ). .. For mammalian expression, LC2, Cav 2.2, and Cav 2.1 constructs were subcloned into pcDNA3.1His (Invitrogen), pCMV-HA vector, pEGFP-C, pIRES-EGFP or pDsRed2-C (Clontech).

    Article Title: Phosphorylation of ATM by Cdk5 mediates DNA damage signaling and regulates neuronal death
    Article Snippet: .. Recombinant GST-fusion proteins were induced and purified from BL21 (DE3) competent cells (Invitrogen). .. Plasmid transfection Primary CGNs were transceintly transfected using Calcium Phosphate ProFection Mammalian Transfection system (Promega) according to the manufacturer’s instructions.

    Article Title: Control of Angiogenesis by AIBP-mediated Cholesterol Efflux
    Article Snippet: .. For expression of AIBP proteins, the genes were cloned in frame into pHUE vector (kindly provided by Tracy Handel), expressed in BL21 DE3 competent cells (Invitrogen) and purified with a Ni-NTA agarose resin column (Qiagen). .. Deubiquitinase (DUB) expressed in pHUE was used as a negative control in experiments with recombinant AIBP.

    Multiple Displacement Amplification:

    Article Title: Metal Ion Assisted Interface Re-engineering of Ferritin Protein Nanocage for Enhanced Biofunctions and Cancer Therapy
    Article Snippet: HT29, SKOV3, U87MG, A549, PC3, and MDA-MB-231 cell lines were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA) and cultured in different media with 10% FBS (HT29 and SKOV3 cells were cultured in McCoy’s 5A media, U87MG and A549 cells were cultured in MEM media, PC3 cells were cultured in F-12 media, and MDA-MB-231 cells were cultured in L-15 media. .. BL21 (DE3) competent cells were bought from Thermo Fisher Scientific.

    Cell Culture:

    Article Title: Metal Ion Assisted Interface Re-engineering of Ferritin Protein Nanocage for Enhanced Biofunctions and Cancer Therapy
    Article Snippet: HT29, SKOV3, U87MG, A549, PC3, and MDA-MB-231 cell lines were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA) and cultured in different media with 10% FBS (HT29 and SKOV3 cells were cultured in McCoy’s 5A media, U87MG and A549 cells were cultured in MEM media, PC3 cells were cultured in F-12 media, and MDA-MB-231 cells were cultured in L-15 media. .. BL21 (DE3) competent cells were bought from Thermo Fisher Scientific.

    Expressing:

    Article Title: Gatekeeping versus Promiscuity in the Early Stages of the Andrimid Biosynthetic Assembly Line
    Article Snippet: E. coli Top10 and BL21 (DE3) competent cells were from Invitrogen. .. Standard protein expression plasmids were obtained from Novagen.

    Article Title: The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI
    Article Snippet: E. coli DH5α and BL21(DE3) competent cells were purchased from Invitrogen (Carlsbad, CA). .. The pET-28a Escherichia coli expression vector was purchased from Novagen (Madison, WI).

    Article Title: THE ROLE OF MAP1A LIGHT CHAIN 2 IN SYNAPTIC SURFACE RETENTION OF CAV2.2 CHANNELS IN HIPPOCAMPAL NEURONS
    Article Snippet: His- and GST-fusion proteins were prepared as bacterial lysates using BL21 (DE3) competent cells (One Shot, Invitrogen) and purified as previously described ( ; ). .. For mammalian expression, LC2, Cav 2.2, and Cav 2.1 constructs were subcloned into pcDNA3.1His (Invitrogen), pCMV-HA vector, pEGFP-C, pIRES-EGFP or pDsRed2-C (Clontech).

    Article Title: Mutagenesis of the regulatory domain of phenylalanine hydroxylase
    Article Snippet: Plasmids pLNC209 ( ) and pLNCB111 ( ) expressing rat and human PAH, respectively, were constructed in this laboratory. .. Escherichia coli strains DH5α and BL21(DE3) competent cells were purchased from Life Technologies (Rockville, MD) and Novagen, respectively.

    Article Title: Control of Angiogenesis by AIBP-mediated Cholesterol Efflux
    Article Snippet: .. For expression of AIBP proteins, the genes were cloned in frame into pHUE vector (kindly provided by Tracy Handel), expressed in BL21 DE3 competent cells (Invitrogen) and purified with a Ni-NTA agarose resin column (Qiagen). .. Deubiquitinase (DUB) expressed in pHUE was used as a negative control in experiments with recombinant AIBP.

    Sequencing:

    Article Title: Parkinson-Related LRRK2 Mutation R1628P Enables Cdk5 Phosphorylation of LRRK2 and Upregulates Its Kinase Activity
    Article Snippet: HA-tagged and GFP-tagged LRRK2 with R1628P, S1627A:R1628P, S1627D, and S1627D:R1628P mutations were performed using QuikChange site-directed II mutagenesis kit (Stratagene), and the point mutations were confirmed by sequencing. .. Recombinant GST-tagged LRRK2-COR protein were induced and purified from BL21 (DE3) competent cells (Invitrogen).

    Article Title: Mutagenesis of the regulatory domain of phenylalanine hydroxylase
    Article Snippet: DNA primers for PCR and sequencing were purchased from Lofstrand Laboratories (Gaithersburg, MD). .. Escherichia coli strains DH5α and BL21(DE3) competent cells were purchased from Life Technologies (Rockville, MD) and Novagen, respectively.

    Western Blot:

    Article Title: THE ROLE OF MAP1A LIGHT CHAIN 2 IN SYNAPTIC SURFACE RETENTION OF CAV2.2 CHANNELS IN HIPPOCAMPAL NEURONS
    Article Snippet: His- and GST-fusion proteins were prepared as bacterial lysates using BL21 (DE3) competent cells (One Shot, Invitrogen) and purified as previously described ( ; ). .. I. Bezprozvanny (UT Southwestern Medical Center, Dallas, TX) and Cav 2.1 in pEGFP-C2 was kindly provided by Dr. S. Herlitze (Case Western Reserve University, Cleveland, OH).

    Transformation Assay:

    Article Title: Real-Time Imaging of Rab5 Activity Using a Pre-Quenched Biosensor
    Article Snippet: .. The pGEX-4T-2-R5BD-BMS and the non-binding mutant (pGEX-4T-2-R5BD(V822D)-BMS) plasmids were transformed into BL21 (DE3) competent cells (Invitrogen) and the recombinant peptides were expressed by induction of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG). .. GST-R5BD fusion proteins were pulled down by glutathione-coated Sepharose 4B beads (GE Healthcare), and conjugated with biotin-Lys(FITC)-Cys(S-S-Dabcyl) through streptavidin.

    Recombinant:

    Article Title: Real-Time Imaging of Rab5 Activity Using a Pre-Quenched Biosensor
    Article Snippet: .. The pGEX-4T-2-R5BD-BMS and the non-binding mutant (pGEX-4T-2-R5BD(V822D)-BMS) plasmids were transformed into BL21 (DE3) competent cells (Invitrogen) and the recombinant peptides were expressed by induction of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG). .. GST-R5BD fusion proteins were pulled down by glutathione-coated Sepharose 4B beads (GE Healthcare), and conjugated with biotin-Lys(FITC)-Cys(S-S-Dabcyl) through streptavidin.

    Article Title: Parkinson-Related LRRK2 Mutation R1628P Enables Cdk5 Phosphorylation of LRRK2 and Upregulates Its Kinase Activity
    Article Snippet: .. Recombinant GST-tagged LRRK2-COR protein were induced and purified from BL21 (DE3) competent cells (Invitrogen). .. Animals Cdk5 conditional knockout (ko) mice in the forebrain were generated by the crossbreed between B6.129S4(Cg)-Cdk5tm1.1Lht/J (Cdk5-loxP) and CamKIIα-Cre mice.

    Article Title: Phosphorylation of ATM by Cdk5 mediates DNA damage signaling and regulates neuronal death
    Article Snippet: .. Recombinant GST-fusion proteins were induced and purified from BL21 (DE3) competent cells (Invitrogen). .. Plasmid transfection Primary CGNs were transceintly transfected using Calcium Phosphate ProFection Mammalian Transfection system (Promega) according to the manufacturer’s instructions.

    Article Title: Control of Angiogenesis by AIBP-mediated Cholesterol Efflux
    Article Snippet: Paragraph title: Cloning of human and zebrafish AIBP, recombinant protein expression and purification, and antibody production ... For expression of AIBP proteins, the genes were cloned in frame into pHUE vector (kindly provided by Tracy Handel), expressed in BL21 DE3 competent cells (Invitrogen) and purified with a Ni-NTA agarose resin column (Qiagen).

    Injection:

    Article Title: Control of Angiogenesis by AIBP-mediated Cholesterol Efflux
    Article Snippet: For expression of AIBP proteins, the genes were cloned in frame into pHUE vector (kindly provided by Tracy Handel), expressed in BL21 DE3 competent cells (Invitrogen) and purified with a Ni-NTA agarose resin column (Qiagen). .. To produce a zAibp2 antibody, recombinant zAibp2 was mixed with complete Freund's adjuvant (Sigma) and injected subcutaneously into a guinea pig.

    Plasmid Preparation:

    Article Title: Parkinson-Related LRRK2 Mutation R1628P Enables Cdk5 Phosphorylation of LRRK2 and Upregulates Its Kinase Activity
    Article Snippet: GST-tagged LRRK2-COR (COR domain, amino acids 1535~1878) was obtained by PCR using FL-HA-LRRK2-WT as template and subcloned to pGEX-2T1 vector (Addgene). .. Recombinant GST-tagged LRRK2-COR protein were induced and purified from BL21 (DE3) competent cells (Invitrogen).

    Article Title: Dihydrophenylalanine: A prephenate-derived Photorhabdus luminescens antibiotic and intermediate in dihydrostilbene biosynthesis
    Article Snippet: E. coli TOP10 and BL21(DE3) competent cells were purchased from Invitrogen. .. Plasmid pET-28b was purchased from Novagen.

    Article Title: The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI
    Article Snippet: E. coli DH5α and BL21(DE3) competent cells were purchased from Invitrogen (Carlsbad, CA). .. The pET-28a Escherichia coli expression vector was purchased from Novagen (Madison, WI).

    Article Title: THE ROLE OF MAP1A LIGHT CHAIN 2 IN SYNAPTIC SURFACE RETENTION OF CAV2.2 CHANNELS IN HIPPOCAMPAL NEURONS
    Article Snippet: His- and GST-fusion proteins were prepared as bacterial lysates using BL21 (DE3) competent cells (One Shot, Invitrogen) and purified as previously described ( ; ). .. For mammalian expression, LC2, Cav 2.2, and Cav 2.1 constructs were subcloned into pcDNA3.1His (Invitrogen), pCMV-HA vector, pEGFP-C, pIRES-EGFP or pDsRed2-C (Clontech).

    Article Title: Phosphorylation of ATM by Cdk5 mediates DNA damage signaling and regulates neuronal death
    Article Snippet: A plasmid encoding an N-terminal Flag-tagged ATM protein was a kind gift of Dr. Michael Kastan . .. Recombinant GST-fusion proteins were induced and purified from BL21 (DE3) competent cells (Invitrogen).

    Article Title: Control of Angiogenesis by AIBP-mediated Cholesterol Efflux
    Article Snippet: .. For expression of AIBP proteins, the genes were cloned in frame into pHUE vector (kindly provided by Tracy Handel), expressed in BL21 DE3 competent cells (Invitrogen) and purified with a Ni-NTA agarose resin column (Qiagen). .. Deubiquitinase (DUB) expressed in pHUE was used as a negative control in experiments with recombinant AIBP.

    DNA Sequencing:

    Article Title: Mutagenesis of the regulatory domain of phenylalanine hydroxylase
    Article Snippet: DNA sequencing was carried out by using the T7 DNA polymerase kit (Amersham Pharmacia) on an automatic DNA sequencer (ALF DNA analysis system, Amersham Pharmacia). .. Escherichia coli strains DH5α and BL21(DE3) competent cells were purchased from Life Technologies (Rockville, MD) and Novagen, respectively.

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    Thermo Fisher one shot bl21 star
    One Shot Bl21 Star, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one shot bl21 star/product/Thermo Fisher
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    one shot bl21 star - by Bioz Stars, 2020-02
    90/100 stars
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