Structured Review

Millipore bl21 de3 chemically competent cells
SDS–PAGE analysis showing expression and purification of Syn Laspo. Lane 1: molecular weight markers with sizes in kDa. Lane 2: non-induced <t>cells</t> of E. coli <t>BL21</t> <t>(DE3)</t> expressing Laspo ( nadB gene). Lane 3: induced cells of E. coli BL21 (DE3) expressing Syn Laspo protein. Lane 4: representative fraction of Syn Laspo peak eluted from Strep-Tactin affinity chromatography. Lane 5: diluted fraction of Syn Laspo peak. Size of the eluted protein correspond to Laspo’s MW of ~60 kDa and protein were judged to be over 90% pure.
Bl21 De3 Chemically Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bl21 de3 chemically competent cells/product/Millipore
Average 95 stars, based on 40 article reviews
Price from $9.99 to $1999.99
bl21 de3 chemically competent cells - by Bioz Stars, 2022-11
95/100 stars

Images

1) Product Images from "L-Aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803"

Article Title: L-Aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

Journal: bioRxiv

doi: 10.1101/2022.10.19.512830

SDS–PAGE analysis showing expression and purification of Syn Laspo. Lane 1: molecular weight markers with sizes in kDa. Lane 2: non-induced cells of E. coli BL21 (DE3) expressing Laspo ( nadB gene). Lane 3: induced cells of E. coli BL21 (DE3) expressing Syn Laspo protein. Lane 4: representative fraction of Syn Laspo peak eluted from Strep-Tactin affinity chromatography. Lane 5: diluted fraction of Syn Laspo peak. Size of the eluted protein correspond to Laspo’s MW of ~60 kDa and protein were judged to be over 90% pure.
Figure Legend Snippet: SDS–PAGE analysis showing expression and purification of Syn Laspo. Lane 1: molecular weight markers with sizes in kDa. Lane 2: non-induced cells of E. coli BL21 (DE3) expressing Laspo ( nadB gene). Lane 3: induced cells of E. coli BL21 (DE3) expressing Syn Laspo protein. Lane 4: representative fraction of Syn Laspo peak eluted from Strep-Tactin affinity chromatography. Lane 5: diluted fraction of Syn Laspo peak. Size of the eluted protein correspond to Laspo’s MW of ~60 kDa and protein were judged to be over 90% pure.

Techniques Used: SDS Page, Expressing, Purification, Molecular Weight, Affinity Chromatography

2) Product Images from "Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication"

Article Title: Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication

Journal: Antioxidants

doi: 10.3390/antiox11101952

The heavy metal-resistant and DNA protection ability of CiPrx3. ( A ) The heavy metal-resistant ability of CiPrx3 . E. coli BL21 strains were transformed with the pEASY- CiPrx3 vector or pEASY-Blunt and then treated with different kinds of heavy metal ions. Scale bar = 1 cm. ( B ) Inhibition zone diameter of E. coli after heavy metal stimulation. Data were shown as the mean ± SD ( n = 3). Asterisks represent significant differences (** = p ≤ 0.01). ( C ) Determination of the DNA protection ability of CiPrx3 by MFO assay; 1: pcDNA3.1 without incubation; 2: pcDNA3.1 + FeCl 3 (10 μM); 3: pcDNA3.1 + DTT (10 mM); 4: pcDNA3.1 + MFO mix (10 μM FeCl 3 + 10 mM DTT); 5: pcDNA3.1 + MFO mix + 5 ng of r CiPrx3 ; 6: pcDNA3.1 + MFO mix + 50 ng of r CiPrx3 ; 7: pcDNA3.1 + MFO mix + 500 ng of r CiPrx3 ; 8: pcDNA3.1 + MFO mix + 1 μg of r CiPrx3 ; 9: pcDNA3.1 + MFO mix + 2 μg of r CiPrx3 ; 10: pcDNA3.1 + MFO mix + 4 μg of r CiPrx3 . OC DNA: open circular plasmid DNA; CCC: covalently closed circular DNA.
Figure Legend Snippet: The heavy metal-resistant and DNA protection ability of CiPrx3. ( A ) The heavy metal-resistant ability of CiPrx3 . E. coli BL21 strains were transformed with the pEASY- CiPrx3 vector or pEASY-Blunt and then treated with different kinds of heavy metal ions. Scale bar = 1 cm. ( B ) Inhibition zone diameter of E. coli after heavy metal stimulation. Data were shown as the mean ± SD ( n = 3). Asterisks represent significant differences (** = p ≤ 0.01). ( C ) Determination of the DNA protection ability of CiPrx3 by MFO assay; 1: pcDNA3.1 without incubation; 2: pcDNA3.1 + FeCl 3 (10 μM); 3: pcDNA3.1 + DTT (10 mM); 4: pcDNA3.1 + MFO mix (10 μM FeCl 3 + 10 mM DTT); 5: pcDNA3.1 + MFO mix + 5 ng of r CiPrx3 ; 6: pcDNA3.1 + MFO mix + 50 ng of r CiPrx3 ; 7: pcDNA3.1 + MFO mix + 500 ng of r CiPrx3 ; 8: pcDNA3.1 + MFO mix + 1 μg of r CiPrx3 ; 9: pcDNA3.1 + MFO mix + 2 μg of r CiPrx3 ; 10: pcDNA3.1 + MFO mix + 4 μg of r CiPrx3 . OC DNA: open circular plasmid DNA; CCC: covalently closed circular DNA.

Techniques Used: Transformation Assay, Plasmid Preparation, Inhibition, Incubation, Countercurrent Chromatography

The antioxidant activity of CiPrx3 . ( A , B ) E. coli BL21 strains were transformed with pEASY-Blunt or pEASY- CiPrx3 and cultured in agarose plates treated with different concentrations of H 2 O 2 . Scale bar =1 cm. ( C ) Inhibition zone diameter of E. coli after H 2 O 2 stimulation. ( D ) Confirmation of the overexpression of CiPrx3 in GCO cells by Western blotting. GCO cells were transfected with pcDNA3.1- CiPrx3 or pcDNA3.1 and harvested at 24 h for Western blotting by using an anti-HA antibody. ( E ) Calculation of relative fluorescence intensity of ROS in pcDNA3.1- CiPrx3 or pcDNA3.1 transfected cells. ( F ) CiPrx3 reduced the intracellular ROS caused by H 2 O 2 stimulation. GCO cells were transfected with pcDNA3.1- CiPrx3 or pcDNA3.1 and then treated with H 2 O 2 (0.4 mM) and collected at different time points (1 h, 3 h, and 6 h) to detect the intracellular ROS by flow cytometry. Data were shown as the mean ± SD ( n = 3). Asterisks represent significant differences (** = p ≤ 0.01).
Figure Legend Snippet: The antioxidant activity of CiPrx3 . ( A , B ) E. coli BL21 strains were transformed with pEASY-Blunt or pEASY- CiPrx3 and cultured in agarose plates treated with different concentrations of H 2 O 2 . Scale bar =1 cm. ( C ) Inhibition zone diameter of E. coli after H 2 O 2 stimulation. ( D ) Confirmation of the overexpression of CiPrx3 in GCO cells by Western blotting. GCO cells were transfected with pcDNA3.1- CiPrx3 or pcDNA3.1 and harvested at 24 h for Western blotting by using an anti-HA antibody. ( E ) Calculation of relative fluorescence intensity of ROS in pcDNA3.1- CiPrx3 or pcDNA3.1 transfected cells. ( F ) CiPrx3 reduced the intracellular ROS caused by H 2 O 2 stimulation. GCO cells were transfected with pcDNA3.1- CiPrx3 or pcDNA3.1 and then treated with H 2 O 2 (0.4 mM) and collected at different time points (1 h, 3 h, and 6 h) to detect the intracellular ROS by flow cytometry. Data were shown as the mean ± SD ( n = 3). Asterisks represent significant differences (** = p ≤ 0.01).

Techniques Used: Antioxidant Activity Assay, Transformation Assay, Cell Culture, Inhibition, Over Expression, Western Blot, Transfection, Fluorescence, Flow Cytometry

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    Millipore bl21 de3 chemically competent cells
    Bl21 De3 Chemically Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 chemically competent cells/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 chemically competent cells - by Bioz Stars, 2022-11
    95/100 stars
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