bl21 de3 codon plus ripl cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 de3 codon plus ripl cells
    Bl21 De3 Codon Plus Ripl Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 codon plus ripl cells/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 codon plus ripl cells - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Affinity Chromatography:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: The recombinant SUMO-fusion proteins in the cleared cell lysate were purified using Ni-NTA (Thermo Fisher Scientific, Waltham, MA) affinity chromatography. .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Chromatography:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: Elution fractions containing the purified SUMO-fusion proteins were pooled and incubated overnight with Ulp1 protease ( ) at 4 °C, followed by a second Ni-NTA chromatography to remove the SUMO tag and uncleaved fusion protein. .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Size-exclusion Chromatography:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: The GSDMD-C protein samples were further purified through size-exclusion chromatography in a buffer containing the lysis buffer plus 5 mM DTT. .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Cell Culture:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein. .. Screening of crystallization conditions for both hGSDMD-C and mGSDMD-C was performed using commercial screening kits from Hampton Research (Aliso Viejo, CA) and Molecular Dimensions (Maumee, OH) with sitting drop vapor diffusion method at 22 °C or 4 °C.

    Purification:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein. .. Screening of crystallization conditions for both hGSDMD-C and mGSDMD-C was performed using commercial screening kits from Hampton Research (Aliso Viejo, CA) and Molecular Dimensions (Maumee, OH) with sitting drop vapor diffusion method at 22 °C or 4 °C.

    Incubation:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: Elution fractions containing the purified SUMO-fusion proteins were pooled and incubated overnight with Ulp1 protease ( ) at 4 °C, followed by a second Ni-NTA chromatography to remove the SUMO tag and uncleaved fusion protein. .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Expressing:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: Paragraph title: Protein expression and purification ... The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Lysis:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: The GSDMD-C protein samples were further purified through size-exclusion chromatography in a buffer containing the lysis buffer plus 5 mM DTT. .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Sonication:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: Cells were harvested and lysed by sonication in a lysis buffer containing 25 mM Tris-HCl (pH 8.0), 300 mM NaCl. .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Transformation Assay:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: The transformed BL21 (DE3) Codon Plus RIPL cells (Agilent Technologies, Santa Clara, CA) were grown at 37 °C and protein expression was induced at 18 °C overnight with 0.2 mM IPTG. .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Recombinant:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: The recombinant SUMO-fusion proteins in the cleared cell lysate were purified using Ni-NTA (Thermo Fisher Scientific, Waltham, MA) affinity chromatography. .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein.

    Derivative Assay:

    Article Title: Structures of the gasdermin D C-terminal domains reveal mechanisms of autoinhibition
    Article Snippet: .. The Se-Met derived mGSDMD-C was expressed using the BL21 (DE3) Codon Plus RIPL cells cultured in SelenoMet medium base plus nutrient mix (MD12-501, Molecular Dimensions, Maumee, OH) with the addition of L-selenomethionine (Thermo Fisher Scientific, Waltham, MA), followed by purification protocols as described for the wild-type protein. .. Screening of crystallization conditions for both hGSDMD-C and mGSDMD-C was performed using commercial screening kits from Hampton Research (Aliso Viejo, CA) and Molecular Dimensions (Maumee, OH) with sitting drop vapor diffusion method at 22 °C or 4 °C.

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  • 99
    Thermo Fisher bl21 de3 codon plus ripl cells
    Bl21 De3 Codon Plus Ripl Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 codon plus ripl cells/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 codon plus ripl cells - by Bioz Stars, 2020-02
    99/100 stars
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