bl21 ai bacteria  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bl21 ai bacteria
    Bl21 Ai Bacteria, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 ai bacteria/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bl21 ai bacteria - by Bioz Stars, 2020-02
    79/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: .. MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen). .. Expression was induced using a final concentration of 0.2% L-arabinose (w/v) and inducing at 27°C with constant rotation for 4 hours.

    Sonication:

    Article Title: Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments
    Article Snippet: GST pull-down assays Protein expressions of glutathione S-transferase (GST) alone or fused to ankyrin repeats domain (GST-ARD) were optimized under L-arabinose induction in BL21-AI bacteria (Invitrogen). .. GFP-myosin IIIa ΔK, - pre3THDI, -3THDI, and -post3THDI proteins were extracted from 24 h COS-7 transfectants via brief sonication and 20-min ultracentrifugation at 145,000 g in ice-cold lysis buffer (1% Triton X-100, 5 mM DTT, 150 mM NaCl, 50 mM Tris pH 7.4, 2 mM EDTA, 3 mM Pefabloc SC, 1x Pefabloc (Roche, Indianapolis, IN), and 1x Mammalian Protease Inhibitor cocktail (Sigma).

    Phosphatase Assay:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen). .. Phosphatase assays were performed using ∼100 ng of each recombinant protein, which was spiked into phosphatase assay buffer (50 mM sodium acetate, 25 mM Tris-HCl, 1 mM DTT, pH 6.0 for PTPMT1, pH 7.0 for MKP-3) and 100 mM O-methylfluoroscien-phosphate (OMFP, Sigma-Aldrich).

    Protease Inhibitor:

    Article Title: Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments
    Article Snippet: GST pull-down assays Protein expressions of glutathione S-transferase (GST) alone or fused to ankyrin repeats domain (GST-ARD) were optimized under L-arabinose induction in BL21-AI bacteria (Invitrogen). .. GFP-myosin IIIa ΔK, - pre3THDI, -3THDI, and -post3THDI proteins were extracted from 24 h COS-7 transfectants via brief sonication and 20-min ultracentrifugation at 145,000 g in ice-cold lysis buffer (1% Triton X-100, 5 mM DTT, 150 mM NaCl, 50 mM Tris pH 7.4, 2 mM EDTA, 3 mM Pefabloc SC, 1x Pefabloc (Roche, Indianapolis, IN), and 1x Mammalian Protease Inhibitor cocktail (Sigma).

    Purification:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen). .. Proteins were purified using glutathione-conjugated sepharose and stored at −80°C until use.

    Article Title: Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments
    Article Snippet: GST pull-down assays Protein expressions of glutathione S-transferase (GST) alone or fused to ankyrin repeats domain (GST-ARD) were optimized under L-arabinose induction in BL21-AI bacteria (Invitrogen). .. GST proteins were purified from bacterial extracts using glutathione–Sepharose 4B beads according to manufacturer’s instructions (Amersham Biosciences, Buckinghamshire, UK).

    Electrophoresis:

    Article Title: Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments
    Article Snippet: GST pull-down assays Protein expressions of glutathione S-transferase (GST) alone or fused to ankyrin repeats domain (GST-ARD) were optimized under L-arabinose induction in BL21-AI bacteria (Invitrogen). .. To test for myosin IIIa interactions, the same amount of GST-ARD or GST alone was bound to 4B beads for 1 h at 4°C followed by incubation with the GFP-tagged myosin IIIa fragment in CLB for 2 h. The beads were then washed four times with lysis buffer, and bound proteins separated by electrophoresis on NuPAGE Bis-Tris 4–12% gels (Invitrogen), and analyzed by Western blotting using rabbit polyclonal anti-GFP and anti-GST antibodies (Invitrogen).

    Concentration Assay:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen). .. Expression was induced using a final concentration of 0.2% L-arabinose (w/v) and inducing at 27°C with constant rotation for 4 hours.

    Incubation:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: Protein expression was induced using isopropyl β-D-1-thiogalactopyranoside (IPTG) and subsequent incubation at 23°C with constant rotation overnight. .. MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen).

    Article Title: Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments
    Article Snippet: GST pull-down assays Protein expressions of glutathione S-transferase (GST) alone or fused to ankyrin repeats domain (GST-ARD) were optimized under L-arabinose induction in BL21-AI bacteria (Invitrogen). .. To test for myosin IIIa interactions, the same amount of GST-ARD or GST alone was bound to 4B beads for 1 h at 4°C followed by incubation with the GFP-tagged myosin IIIa fragment in CLB for 2 h. The beads were then washed four times with lysis buffer, and bound proteins separated by electrophoresis on NuPAGE Bis-Tris 4–12% gels (Invitrogen), and analyzed by Western blotting using rabbit polyclonal anti-GFP and anti-GST antibodies (Invitrogen).

    Expressing:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: Protein expression was induced using isopropyl β-D-1-thiogalactopyranoside (IPTG) and subsequent incubation at 23°C with constant rotation overnight. .. MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen).

    Lysis:

    Article Title: Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments
    Article Snippet: GST pull-down assays Protein expressions of glutathione S-transferase (GST) alone or fused to ankyrin repeats domain (GST-ARD) were optimized under L-arabinose induction in BL21-AI bacteria (Invitrogen). .. GFP-myosin IIIa ΔK, - pre3THDI, -3THDI, and -post3THDI proteins were extracted from 24 h COS-7 transfectants via brief sonication and 20-min ultracentrifugation at 145,000 g in ice-cold lysis buffer (1% Triton X-100, 5 mM DTT, 150 mM NaCl, 50 mM Tris pH 7.4, 2 mM EDTA, 3 mM Pefabloc SC, 1x Pefabloc (Roche, Indianapolis, IN), and 1x Mammalian Protease Inhibitor cocktail (Sigma).

    Sequencing:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: .. MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen). .. Expression was induced using a final concentration of 0.2% L-arabinose (w/v) and inducing at 27°C with constant rotation for 4 hours.

    Western Blot:

    Article Title: Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments
    Article Snippet: GST pull-down assays Protein expressions of glutathione S-transferase (GST) alone or fused to ankyrin repeats domain (GST-ARD) were optimized under L-arabinose induction in BL21-AI bacteria (Invitrogen). .. To test for myosin IIIa interactions, the same amount of GST-ARD or GST alone was bound to 4B beads for 1 h at 4°C followed by incubation with the GFP-tagged myosin IIIa fragment in CLB for 2 h. The beads were then washed four times with lysis buffer, and bound proteins separated by electrophoresis on NuPAGE Bis-Tris 4–12% gels (Invitrogen), and analyzed by Western blotting using rabbit polyclonal anti-GFP and anti-GST antibodies (Invitrogen).

    Transformation Assay:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: .. MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen). .. Expression was induced using a final concentration of 0.2% L-arabinose (w/v) and inducing at 27°C with constant rotation for 4 hours.

    Recombinant:

    Article Title: Downregulation of the Mitochondrial Phosphatase PTPMT1 Is Sufficient to Promote Cancer Cell Death
    Article Snippet: Paragraph title: Preparation of Recombinant Proteins and Phosphatase Assays ... MKP-3 was cloned into pDEST15 (Invitrogen), sequence verified, and transformed into BL21-AI bacteria (Invitrogen).

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    Thermo Fisher bl21 ai e coli
    Bl21 Ai E Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 ai e coli/product/Thermo Fisher
    Average 97 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bl21 ai e coli - by Bioz Stars, 2020-02
    97/100 stars
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