bisulfite treated dna  (Millipore)


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  • 99
    Name:
    Deoxyribonucleic acid
    Description:
    Human placental DNA is isolated from donor placenta but will contain some maternal DNA The DNA fragments are sonicated to produce fragments of consistent size
    Catalog Number:
    d3287
    Price:
    None
    Applications:
    Sonicated Deoxyribonucleic acid, single stranded from human placenta, was used as blocking agent in Southern hybridization of DNA from human papillomavirus (HPV) positive SiHa, HeLa and CaSki cell-lines. It was used as standard in GC/MS analysis of exocyclic DNA adducts.
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    Structured Review

    Millipore bisulfite treated dna
    The Recruitment of CHD4 to Oxidative <t>DNA</t> Damage Sites Depends on OGG1 (A) CoIPs of lysates from SW480 cells untreated or treated with 2 mM H 2 O 2 for 30 min were performed with the indicated antibodies. (B) Purified OGG1 and Flag-CHD4 were incubated with antibodies against Flag or OGG1 in IP buffer. The immunoprecipitated samples were detected by western blot analyses using the antibodies indicated. (C) After SW480 OGG1 KO cells were transfected with pCMV-Taq or pCMV-OGG1 for 48 hr, the cells were untreated or treated with 2mMH 2 O 2 for 30 min. Whole-cell extracts and the tight chromatin fractions were analyzed by immunoblotting with the indicated antibodies. (D) Whole-cell extracts and the tight chromatin fractions from SW480 CHD4 KD cells untreated (Un) or treated with 2 mM H 2 O 2 for 30 min were analyzed by immunoblotting as in (C). (E) Purified OGG1 and Flag-CHD4 were incubated with antibodies against Flag or OGG1 in IP buffer with or without 8-OHdG oligonucleotide. The immuno-precipitated samples were detected by western blot analyses using the antibodies indicated. (F) Biotin labeled 8-OHdG oligonucleotide incubated with OGG1 and Flag-CHD4 was pulled down using streptavidin beads. Bound proteins were eluted and analyzed by immunoblotting with the indicated antibodies. (G) SW480 OGG1 KO cells were untreated or treated with 2mMH 2 O 2 for 30 min followed by ChIP for control IgG, 8-OHdG, and CHD4 at the promoter CpG islands of eight representative genes and analyzed by real-time <t>RT-PCR.</t> Data are represented as mean ± SEM for triplicate experiments. (H) Cells were untreated or treated with 2 mM H 2 O 2 for 30 min. Sequential ChIP analyses were performed to test the co-occupancy of CHD4 and 8-OHdG at the promoter CpG islands of eight TSGs. Data are represented as mean ± SEM for triplicate experiments. (I) Cells were untreated or treated with 2mMH 2 O 2 for 30 min, and nascent RNA was labeled concurrently. Real-time RT-PCR data are presented as mean ± SEM of the treated over untreated values for triplicate experiments. (J) Sequential ChIP analyses were performed to test the co-occupancy of CHD4 and 8-OHdG or epigenetic silencing proteins at the promoter CpG islands of eight representative TSGs in fresh frozen human CRC tissues (n = 20) and normal colon epithelial tissues (n = 6). Data are represented as mean ± SEM. .
    Human placental DNA is isolated from donor placenta but will contain some maternal DNA The DNA fragments are sonicated to produce fragments of consistent size
    https://www.bioz.com/result/bisulfite treated dna/product/Millipore
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    bisulfite treated dna - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes"

    Article Title: CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes

    Journal: Cancer cell

    doi: 10.1016/j.ccell.2017.04.005

    The Recruitment of CHD4 to Oxidative DNA Damage Sites Depends on OGG1 (A) CoIPs of lysates from SW480 cells untreated or treated with 2 mM H 2 O 2 for 30 min were performed with the indicated antibodies. (B) Purified OGG1 and Flag-CHD4 were incubated with antibodies against Flag or OGG1 in IP buffer. The immunoprecipitated samples were detected by western blot analyses using the antibodies indicated. (C) After SW480 OGG1 KO cells were transfected with pCMV-Taq or pCMV-OGG1 for 48 hr, the cells were untreated or treated with 2mMH 2 O 2 for 30 min. Whole-cell extracts and the tight chromatin fractions were analyzed by immunoblotting with the indicated antibodies. (D) Whole-cell extracts and the tight chromatin fractions from SW480 CHD4 KD cells untreated (Un) or treated with 2 mM H 2 O 2 for 30 min were analyzed by immunoblotting as in (C). (E) Purified OGG1 and Flag-CHD4 were incubated with antibodies against Flag or OGG1 in IP buffer with or without 8-OHdG oligonucleotide. The immuno-precipitated samples were detected by western blot analyses using the antibodies indicated. (F) Biotin labeled 8-OHdG oligonucleotide incubated with OGG1 and Flag-CHD4 was pulled down using streptavidin beads. Bound proteins were eluted and analyzed by immunoblotting with the indicated antibodies. (G) SW480 OGG1 KO cells were untreated or treated with 2mMH 2 O 2 for 30 min followed by ChIP for control IgG, 8-OHdG, and CHD4 at the promoter CpG islands of eight representative genes and analyzed by real-time RT-PCR. Data are represented as mean ± SEM for triplicate experiments. (H) Cells were untreated or treated with 2 mM H 2 O 2 for 30 min. Sequential ChIP analyses were performed to test the co-occupancy of CHD4 and 8-OHdG at the promoter CpG islands of eight TSGs. Data are represented as mean ± SEM for triplicate experiments. (I) Cells were untreated or treated with 2mMH 2 O 2 for 30 min, and nascent RNA was labeled concurrently. Real-time RT-PCR data are presented as mean ± SEM of the treated over untreated values for triplicate experiments. (J) Sequential ChIP analyses were performed to test the co-occupancy of CHD4 and 8-OHdG or epigenetic silencing proteins at the promoter CpG islands of eight representative TSGs in fresh frozen human CRC tissues (n = 20) and normal colon epithelial tissues (n = 6). Data are represented as mean ± SEM. .
    Figure Legend Snippet: The Recruitment of CHD4 to Oxidative DNA Damage Sites Depends on OGG1 (A) CoIPs of lysates from SW480 cells untreated or treated with 2 mM H 2 O 2 for 30 min were performed with the indicated antibodies. (B) Purified OGG1 and Flag-CHD4 were incubated with antibodies against Flag or OGG1 in IP buffer. The immunoprecipitated samples were detected by western blot analyses using the antibodies indicated. (C) After SW480 OGG1 KO cells were transfected with pCMV-Taq or pCMV-OGG1 for 48 hr, the cells were untreated or treated with 2mMH 2 O 2 for 30 min. Whole-cell extracts and the tight chromatin fractions were analyzed by immunoblotting with the indicated antibodies. (D) Whole-cell extracts and the tight chromatin fractions from SW480 CHD4 KD cells untreated (Un) or treated with 2 mM H 2 O 2 for 30 min were analyzed by immunoblotting as in (C). (E) Purified OGG1 and Flag-CHD4 were incubated with antibodies against Flag or OGG1 in IP buffer with or without 8-OHdG oligonucleotide. The immuno-precipitated samples were detected by western blot analyses using the antibodies indicated. (F) Biotin labeled 8-OHdG oligonucleotide incubated with OGG1 and Flag-CHD4 was pulled down using streptavidin beads. Bound proteins were eluted and analyzed by immunoblotting with the indicated antibodies. (G) SW480 OGG1 KO cells were untreated or treated with 2mMH 2 O 2 for 30 min followed by ChIP for control IgG, 8-OHdG, and CHD4 at the promoter CpG islands of eight representative genes and analyzed by real-time RT-PCR. Data are represented as mean ± SEM for triplicate experiments. (H) Cells were untreated or treated with 2 mM H 2 O 2 for 30 min. Sequential ChIP analyses were performed to test the co-occupancy of CHD4 and 8-OHdG at the promoter CpG islands of eight TSGs. Data are represented as mean ± SEM for triplicate experiments. (I) Cells were untreated or treated with 2mMH 2 O 2 for 30 min, and nascent RNA was labeled concurrently. Real-time RT-PCR data are presented as mean ± SEM of the treated over untreated values for triplicate experiments. (J) Sequential ChIP analyses were performed to test the co-occupancy of CHD4 and 8-OHdG or epigenetic silencing proteins at the promoter CpG islands of eight representative TSGs in fresh frozen human CRC tissues (n = 20) and normal colon epithelial tissues (n = 6). Data are represented as mean ± SEM. .

    Techniques Used: Purification, Incubation, Immunoprecipitation, Western Blot, Transfection, Labeling, Chromatin Immunoprecipitation, Quantitative RT-PCR

    2) Product Images from "Genetic and Epigenetic Modifications of Sox2 Contribute to the Invasive Phenotype of Malignant Gliomas"

    Article Title: Genetic and Epigenetic Modifications of Sox2 Contribute to the Invasive Phenotype of Malignant Gliomas

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0026740

    Methylation analysis of the Sox2 promoter gene. A. Analysis of the Sox2 promoter CpG content. The position of the Sox2 promoter CpG island is shown. The vertical tic marks denote the CpGs in the island, and the position of the DNA methylation assay with MSP primers is shown. B. MSP analysis of the promoter CpG islands of Sox2 in 12 patients with GBM. PCR products recognizing unmethylated (U) and methylated (M) CpG sites were analyzed on 2% agarose gels. C+ = positive control; in vitro methylated control, C− = negative control; DNA from normal brain and ddH 2 O = water control containing no DNA. C. Relative expression of SOX2 mRNA following treatment with either vehicle (Veh), Trichostatin A (TSA), or 5-aza-2′-deoxycytidine (AZA;). Data were averaged from three independent experiments. Normal human astrocytes (NHAs) and U251 MG cell line were used as negative and positive controls, respectively. D. Analysis of the expression of Sox2 protein following the same treatment described in C. Data were averaged from three independent experiments. The U251 MG cell line was used as positive control.
    Figure Legend Snippet: Methylation analysis of the Sox2 promoter gene. A. Analysis of the Sox2 promoter CpG content. The position of the Sox2 promoter CpG island is shown. The vertical tic marks denote the CpGs in the island, and the position of the DNA methylation assay with MSP primers is shown. B. MSP analysis of the promoter CpG islands of Sox2 in 12 patients with GBM. PCR products recognizing unmethylated (U) and methylated (M) CpG sites were analyzed on 2% agarose gels. C+ = positive control; in vitro methylated control, C− = negative control; DNA from normal brain and ddH 2 O = water control containing no DNA. C. Relative expression of SOX2 mRNA following treatment with either vehicle (Veh), Trichostatin A (TSA), or 5-aza-2′-deoxycytidine (AZA;). Data were averaged from three independent experiments. Normal human astrocytes (NHAs) and U251 MG cell line were used as negative and positive controls, respectively. D. Analysis of the expression of Sox2 protein following the same treatment described in C. Data were averaged from three independent experiments. The U251 MG cell line was used as positive control.

    Techniques Used: Methylation, DNA Methylation Assay, Polymerase Chain Reaction, Positive Control, In Vitro, Negative Control, Expressing

    3) Product Images from "Epigenetically regulated MIR941 and MIR1247 target gastric cancer cell growth and migration"

    Article Title: Epigenetically regulated MIR941 and MIR1247 target gastric cancer cell growth and migration

    Journal: Epigenetics

    doi: 10.4161/epi.29007

    Figure 2. Methylation analysis of MIR941 and MIR1247 in gastric cancer cell lines. ( A ) Schematic representation of MIR941 and MIR1247 CpG island (dotted box). Both miRNAs are embedded into the CpG island (gray box). The regions analyzed using methylation specific PCR (MSP), bisulfite sequencing are indicated by black bars below the CpG island. ( B ) MSP analysis of MIR941 and MIR1247 in four gastric cancer cell lines. In vitro methylated DNA (IVD) and DKO cells were used as positive and negative controls, respectively. U and M indicates unmethylation and methylation signals, respectively. ( C ) Representative bisulfite sequencing analysis was performed for MIR941 and MIR1247 in gastric cancer cell lines (AGS, NCI-N78, and KATO III) as well as cells treated with 5-aza-dC. Each box represents a CpG dinucleotide. Black boxes represent methylated cytosines while white boxes represent unmethylated cytosines.
    Figure Legend Snippet: Figure 2. Methylation analysis of MIR941 and MIR1247 in gastric cancer cell lines. ( A ) Schematic representation of MIR941 and MIR1247 CpG island (dotted box). Both miRNAs are embedded into the CpG island (gray box). The regions analyzed using methylation specific PCR (MSP), bisulfite sequencing are indicated by black bars below the CpG island. ( B ) MSP analysis of MIR941 and MIR1247 in four gastric cancer cell lines. In vitro methylated DNA (IVD) and DKO cells were used as positive and negative controls, respectively. U and M indicates unmethylation and methylation signals, respectively. ( C ) Representative bisulfite sequencing analysis was performed for MIR941 and MIR1247 in gastric cancer cell lines (AGS, NCI-N78, and KATO III) as well as cells treated with 5-aza-dC. Each box represents a CpG dinucleotide. Black boxes represent methylated cytosines while white boxes represent unmethylated cytosines.

    Techniques Used: Methylation, Polymerase Chain Reaction, Methylation Sequencing, In Vitro

    4) Product Images from "Absence of E-Cadherin Expression Distinguishes Noncohesive from Cohesive Pancreatic Cancer"

    Article Title: Absence of E-Cadherin Expression Distinguishes Noncohesive from Cohesive Pancreatic Cancer

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    doi: 10.1158/1078-0432.CCR-07-0487

    Unmethylated ( U )-specific ( lanes1, 3, 5, 7 , and 9 ) and methylation ( M )-specific ( lanes 2, 4, 6, 8 , and 10 ) PCR amplification of the CpG island in the E-cadherin promoter. Bisulfite-treated water and in vitro – methylated DNA served as negative and positive controls, respectively. A, MiaPaca-2 [undifferentiated ( Undiff. ) pancreatic cancer], AsPC-1 [differentiated ( Diff. ) pancreatic cancer], and BxPC-3 (differentiated pancreatic cancer). B, noncohesive (i.e., undifferentiated) and cohesive (i.e., differentiated) foci from three different pancreatic cancers were separated by macrodissecting frozen tumor according to the microscopic appearance of a histologic section from each frozen tumor sample. Neg., negative; Pos., positive.
    Figure Legend Snippet: Unmethylated ( U )-specific ( lanes1, 3, 5, 7 , and 9 ) and methylation ( M )-specific ( lanes 2, 4, 6, 8 , and 10 ) PCR amplification of the CpG island in the E-cadherin promoter. Bisulfite-treated water and in vitro – methylated DNA served as negative and positive controls, respectively. A, MiaPaca-2 [undifferentiated ( Undiff. ) pancreatic cancer], AsPC-1 [differentiated ( Diff. ) pancreatic cancer], and BxPC-3 (differentiated pancreatic cancer). B, noncohesive (i.e., undifferentiated) and cohesive (i.e., differentiated) foci from three different pancreatic cancers were separated by macrodissecting frozen tumor according to the microscopic appearance of a histologic section from each frozen tumor sample. Neg., negative; Pos., positive.

    Techniques Used: Methylation, Polymerase Chain Reaction, Amplification, In Vitro

    Related Articles

    DNA Extraction:

    Article Title: TNFα Amplifies DNaseI Expression in Renal Tubular Cells while IL-1β Promotes Nuclear DNaseI Translocation in an Endonuclease-Inactive Form
    Article Snippet: .. DNA isolation and electrophoretic analysis Total DNA was isolated from RPTEC without and with TNFα- (20ng/ml) stimulation for 48 hrs by using GenElute Mammalian Genomic DNA Miniprep kit from Sigma Aldrich (St. Louis, Missouri, USA). .. One μg of total DNA was size-fractionated by gel electrophoresis in 1.5% SeaKem LE Agarose gel from Lonza (Rockland, ME USA) in Tris-acetate-EDTA buffer and visualized under UV illuminator using staining with Nucleic Acid Gel Stains GelRed 730–2958 (VWR International, Oslo, Norway).

    Whole Genome Amplification:

    Article Title: Next Generation Sequencing-Based Comprehensive Chromosome Screening in Mouse Polar Bodies, Oocytes, and Embryos 1
    Article Snippet: .. WGA DNA was purified using GenElute PCR cleanup columns (Sigma-Aldrich) and quantified using a Nanodrop 8000 spectrophotometer (Fisher Scientific Inc., Waltham, MA). .. WGA DNA was normalized to 200 ng in a total volume of 35 μl of molecular biological grade water (Lonza, Rockland, ME).

    Isolation:

    Article Title: TNFα Amplifies DNaseI Expression in Renal Tubular Cells while IL-1β Promotes Nuclear DNaseI Translocation in an Endonuclease-Inactive Form
    Article Snippet: .. DNA isolation and electrophoretic analysis Total DNA was isolated from RPTEC without and with TNFα- (20ng/ml) stimulation for 48 hrs by using GenElute Mammalian Genomic DNA Miniprep kit from Sigma Aldrich (St. Louis, Missouri, USA). .. One μg of total DNA was size-fractionated by gel electrophoresis in 1.5% SeaKem LE Agarose gel from Lonza (Rockland, ME USA) in Tris-acetate-EDTA buffer and visualized under UV illuminator using staining with Nucleic Acid Gel Stains GelRed 730–2958 (VWR International, Oslo, Norway).

    Spectrophotometry:

    Article Title: Next Generation Sequencing-Based Comprehensive Chromosome Screening in Mouse Polar Bodies, Oocytes, and Embryos 1
    Article Snippet: .. WGA DNA was purified using GenElute PCR cleanup columns (Sigma-Aldrich) and quantified using a Nanodrop 8000 spectrophotometer (Fisher Scientific Inc., Waltham, MA). .. WGA DNA was normalized to 200 ng in a total volume of 35 μl of molecular biological grade water (Lonza, Rockland, ME).

    Purification:

    Article Title: Cellular Uptake of Tile-Assembled DNA Nanotubes
    Article Snippet: .. Purification of the assembled DNA nanotubes was done using 30K Amicon Ultra 0.5-mL centrifuge filters (30000 MWCO, Millipore, Schwalbach, Germany) to remove excess strands that were not folded into the structures. .. One hundred microliters of assembled DNA nanotube solution were mixed with 400 µL of folding buffer, filled into the centrifuge filter, and centrifuged 3 times at 13,000× g for 6 min. After every centrifuge step, the flow-through was removed and the filter was refilled up to 500 µL with buffer.

    Article Title: Next Generation Sequencing-Based Comprehensive Chromosome Screening in Mouse Polar Bodies, Oocytes, and Embryos 1
    Article Snippet: .. WGA DNA was purified using GenElute PCR cleanup columns (Sigma-Aldrich) and quantified using a Nanodrop 8000 spectrophotometer (Fisher Scientific Inc., Waltham, MA). .. WGA DNA was normalized to 200 ng in a total volume of 35 μl of molecular biological grade water (Lonza, Rockland, ME).

    Immunoprecipitation:

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Lytic Switch Protein Stimulates DNA Binding of RBP-Jk/CSL To Activate the Notch Pathway
    Article Snippet: .. For BCBL-1, 293, BL-41, or BJAB cells, protein-DNA complexes were immunoprecipitated as described above or with anti-VP16 antibody (Sigma). .. Immunoprecipitated complexes were collected with 60 μl of 50% (vol/vol) protein A agarose for 2 h at 4°C.

    Polymerase Chain Reaction:

    Article Title: Next Generation Sequencing-Based Comprehensive Chromosome Screening in Mouse Polar Bodies, Oocytes, and Embryos 1
    Article Snippet: .. WGA DNA was purified using GenElute PCR cleanup columns (Sigma-Aldrich) and quantified using a Nanodrop 8000 spectrophotometer (Fisher Scientific Inc., Waltham, MA). .. WGA DNA was normalized to 200 ng in a total volume of 35 μl of molecular biological grade water (Lonza, Rockland, ME).

    Injection:

    Article Title: HMGB1 Promotes Systemic Lupus Erythematosus by Enhancing Macrophage Inflammatory Response
    Article Snippet: .. To generate the murine model of SLE, six-week-old female BALB/c mice were immunized s.c. with ALD-DNA (50 μ g/mouse) plus CFA (Sigma-Aldrich) on day 1, followed by s.c. injection of ALD-DNA (50 μ g/mouse) emulsified with CFA (Sigma-Aldrich) on days 14 and 28 for total of three times as described previously [ – ]. .. Mice in each group received an equal volume of PBS plus CFA or IFA, or UnALD-DNA (50 mg/mouse) plus CFA or IFA were used as controls.

    Mouse Assay:

    Article Title: HMGB1 Promotes Systemic Lupus Erythematosus by Enhancing Macrophage Inflammatory Response
    Article Snippet: .. To generate the murine model of SLE, six-week-old female BALB/c mice were immunized s.c. with ALD-DNA (50 μ g/mouse) plus CFA (Sigma-Aldrich) on day 1, followed by s.c. injection of ALD-DNA (50 μ g/mouse) emulsified with CFA (Sigma-Aldrich) on days 14 and 28 for total of three times as described previously [ – ]. .. Mice in each group received an equal volume of PBS plus CFA or IFA, or UnALD-DNA (50 mg/mouse) plus CFA or IFA were used as controls.

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  • 99
    Millipore bisulfite treated dna
    Bisulfite Treated Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bisulfite treated dna/product/Millipore
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    bisulfite treated dna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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