Structured Review

ATUM bisulfite treated dna
Bisulfite Treated Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bisulfite treated dna/product/ATUM
Average 93 stars, based on 14 article reviews
Price from $9.99 to $1999.99
bisulfite treated dna - by Bioz Stars, 2020-09
93/100 stars

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Polymerase Chain Reaction:

Article Title: Genome-wide survey reveals dynamic widespread tissue-specific changes in DNA methylation during development
Article Snippet: .. Amplification of 1 μl bisulfite-treated DNA (~20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 μl reaction volume using PCR primers at a 200 nM final concentration. .. PCR amplification was performed with the following parameters: 94 °C for 15 min hot start, followed by denaturing at 94 °C for 20 s, annealing at 56 °C for 30 s, extension at 72 °C for 1 min for 45 cycles, and final incubation at 72 °C for 3 min. PCR products were processed for MassARRAY analysis according to the manufacturer's instructions (Sequenom hMC) by the Microarray and Genomics Core Facility at Roswell Park Cancer Institute.

Article Title: DNA hypermethylation of zygote arrest 1 (ZAR1) in hepatitis C virus positive related hepatocellular carcinoma
Article Snippet: .. PCR amplification was performed using HotStar Taq Polymerase (Qiagen) in a 5-μl reaction volume using PCR primer at a final concentration of 200 nM and 1 μl of bisulfite-treated DNA (~20 ng/ml). .. After treatment with shrimp alkaline phosphatase, 2-μl aliquots of the PCR products were used as a template for in vitro transcription and RNase A cleavage for the T-reverse reaction, as described in the manufacturer’s instructions (Sequenom hMC).

Article Title: Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome
Article Snippet: .. Briefly, PCR reactions were carried out in a 25 μL final volume comprising 12.5 μL of PyroMark Master Mix, 2.5 μL of CoralLoad buffer, 0.5 μL of forward and biotinylated reverse primers (0.2 μM final concentration), 8 μL of RNase-free water, and 1 μL of bisulfite-treated DNA (20 ng). .. The biotinylated PCR products were processed as described elsewhere [ ].

Article Title: Quantitative analysis of human tissue-specific differences in methylation
Article Snippet: .. PCR amplification of 1 ul bisulfite treated DNA (~20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 ul reaction volume using PCR primers at a 200 nM final concentration. .. After Shrimp Alkaline Phosphatase treatment, 2 ul of the PCR products were used as a template for in vitro transcription and RNase A Cleavage for the T-reverse reaction, as per manufacturer’s instructions (Sequenom hMC).

Article Title: Promoter CpG island methylation of RET predicts poor prognosis in stage II colorectal cancer patients), Promoter CpG island methylation of RET predicts poor prognosis in stage II colorectal cancer patients
Article Snippet: .. PCR reactions were carried out in a 25 μL final volume comprising 12.5 μL of PyroMark Master Mix, 2.5 μL of Coral Load buffer, 0.5 μL of forward and biotinylated reverse primers (0.2 μM final concentration), 8 μL of RNase‐free water and 1 μL of bisulfite‐treated DNA (20 ng). .. The biotinylated PCR products were processed as previously described ( ).

Article Title: Tissue specific differentially methylated regions (TDMR): Changes in DNA methylation during development
Article Snippet: .. Amplification of 1 ul bisulfite treated DNA (∼20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 ul reaction volume using PCR primers at a 200 nM final concentration. .. PCR amplification was performed with the following parameters: 94°C for 15 minute hot start, followed by denaturing at 94°C for 20 seconds, annealing at 56°C for 30 seconds, extension at 72°C for one minute for 45 cycles, and final incubation at 72°C for three minutes.

Concentration Assay:

Article Title: Genome-wide survey reveals dynamic widespread tissue-specific changes in DNA methylation during development
Article Snippet: .. Amplification of 1 μl bisulfite-treated DNA (~20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 μl reaction volume using PCR primers at a 200 nM final concentration. .. PCR amplification was performed with the following parameters: 94 °C for 15 min hot start, followed by denaturing at 94 °C for 20 s, annealing at 56 °C for 30 s, extension at 72 °C for 1 min for 45 cycles, and final incubation at 72 °C for 3 min. PCR products were processed for MassARRAY analysis according to the manufacturer's instructions (Sequenom hMC) by the Microarray and Genomics Core Facility at Roswell Park Cancer Institute.

Article Title: DNA hypermethylation of zygote arrest 1 (ZAR1) in hepatitis C virus positive related hepatocellular carcinoma
Article Snippet: .. PCR amplification was performed using HotStar Taq Polymerase (Qiagen) in a 5-μl reaction volume using PCR primer at a final concentration of 200 nM and 1 μl of bisulfite-treated DNA (~20 ng/ml). .. After treatment with shrimp alkaline phosphatase, 2-μl aliquots of the PCR products were used as a template for in vitro transcription and RNase A cleavage for the T-reverse reaction, as described in the manufacturer’s instructions (Sequenom hMC).

Article Title: Discovery of Epigenetic Biomarkers for the Non-Invasive Diagnosis of Fetal Disease
Article Snippet: .. Amplification of 1 μL bisulfite treated DNA (20 ng/μL) was performed using HotStar Taq Polymerase (Qiagen) and primers at a final concentration of 200nM each in a 5 μL reaction volume using a 384 well plate. .. PCR amplification was performed in a PTC225 thermal cycler (MJ Research, Inc.) with the following parameters: 94°C for 15 min hot start, followed by denaturing at 94°C for 20 sec, annealing at 62°C for 30 sec, extension at 72°C for 1 min for 45 cycles, and final incubation at 72°C for 3 min.

Article Title: Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome
Article Snippet: .. Briefly, PCR reactions were carried out in a 25 μL final volume comprising 12.5 μL of PyroMark Master Mix, 2.5 μL of CoralLoad buffer, 0.5 μL of forward and biotinylated reverse primers (0.2 μM final concentration), 8 μL of RNase-free water, and 1 μL of bisulfite-treated DNA (20 ng). .. The biotinylated PCR products were processed as described elsewhere [ ].

Article Title: Quantitative analysis of human tissue-specific differences in methylation
Article Snippet: .. PCR amplification of 1 ul bisulfite treated DNA (~20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 ul reaction volume using PCR primers at a 200 nM final concentration. .. After Shrimp Alkaline Phosphatase treatment, 2 ul of the PCR products were used as a template for in vitro transcription and RNase A Cleavage for the T-reverse reaction, as per manufacturer’s instructions (Sequenom hMC).

Article Title: Promoter CpG island methylation of RET predicts poor prognosis in stage II colorectal cancer patients), Promoter CpG island methylation of RET predicts poor prognosis in stage II colorectal cancer patients
Article Snippet: .. PCR reactions were carried out in a 25 μL final volume comprising 12.5 μL of PyroMark Master Mix, 2.5 μL of Coral Load buffer, 0.5 μL of forward and biotinylated reverse primers (0.2 μM final concentration), 8 μL of RNase‐free water and 1 μL of bisulfite‐treated DNA (20 ng). .. The biotinylated PCR products were processed as previously described ( ).

Article Title: Tissue specific differentially methylated regions (TDMR): Changes in DNA methylation during development
Article Snippet: .. Amplification of 1 ul bisulfite treated DNA (∼20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 ul reaction volume using PCR primers at a 200 nM final concentration. .. PCR amplification was performed with the following parameters: 94°C for 15 minute hot start, followed by denaturing at 94°C for 20 seconds, annealing at 56°C for 30 seconds, extension at 72°C for one minute for 45 cycles, and final incubation at 72°C for three minutes.

Amplification:

Article Title: Genome-wide survey reveals dynamic widespread tissue-specific changes in DNA methylation during development
Article Snippet: .. Amplification of 1 μl bisulfite-treated DNA (~20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 μl reaction volume using PCR primers at a 200 nM final concentration. .. PCR amplification was performed with the following parameters: 94 °C for 15 min hot start, followed by denaturing at 94 °C for 20 s, annealing at 56 °C for 30 s, extension at 72 °C for 1 min for 45 cycles, and final incubation at 72 °C for 3 min. PCR products were processed for MassARRAY analysis according to the manufacturer's instructions (Sequenom hMC) by the Microarray and Genomics Core Facility at Roswell Park Cancer Institute.

Article Title: DNA hypermethylation of zygote arrest 1 (ZAR1) in hepatitis C virus positive related hepatocellular carcinoma
Article Snippet: .. PCR amplification was performed using HotStar Taq Polymerase (Qiagen) in a 5-μl reaction volume using PCR primer at a final concentration of 200 nM and 1 μl of bisulfite-treated DNA (~20 ng/ml). .. After treatment with shrimp alkaline phosphatase, 2-μl aliquots of the PCR products were used as a template for in vitro transcription and RNase A cleavage for the T-reverse reaction, as described in the manufacturer’s instructions (Sequenom hMC).

Article Title: Discovery of Epigenetic Biomarkers for the Non-Invasive Diagnosis of Fetal Disease
Article Snippet: .. Amplification of 1 μL bisulfite treated DNA (20 ng/μL) was performed using HotStar Taq Polymerase (Qiagen) and primers at a final concentration of 200nM each in a 5 μL reaction volume using a 384 well plate. .. PCR amplification was performed in a PTC225 thermal cycler (MJ Research, Inc.) with the following parameters: 94°C for 15 min hot start, followed by denaturing at 94°C for 20 sec, annealing at 62°C for 30 sec, extension at 72°C for 1 min for 45 cycles, and final incubation at 72°C for 3 min.

Article Title: Quantitative analysis of human tissue-specific differences in methylation
Article Snippet: .. PCR amplification of 1 ul bisulfite treated DNA (~20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 ul reaction volume using PCR primers at a 200 nM final concentration. .. After Shrimp Alkaline Phosphatase treatment, 2 ul of the PCR products were used as a template for in vitro transcription and RNase A Cleavage for the T-reverse reaction, as per manufacturer’s instructions (Sequenom hMC).

Article Title: Tissue specific differentially methylated regions (TDMR): Changes in DNA methylation during development
Article Snippet: .. Amplification of 1 ul bisulfite treated DNA (∼20 ng/ml) was performed using HotStar Taq Polymerase (Qiagen) in a 5 ul reaction volume using PCR primers at a 200 nM final concentration. .. PCR amplification was performed with the following parameters: 94°C for 15 minute hot start, followed by denaturing at 94°C for 20 seconds, annealing at 56°C for 30 seconds, extension at 72°C for one minute for 45 cycles, and final incubation at 72°C for three minutes.