bisulfite treated cpgenome universal methylated dna  (Millipore)


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    Structured Review

    Millipore bisulfite treated cpgenome universal methylated dna
    Bisulfite Treated Cpgenome Universal Methylated Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bisulfite treated cpgenome universal methylated dna/product/Millipore
    Average 88 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    bisulfite treated cpgenome universal methylated dna - by Bioz Stars, 2020-07
    88/100 stars

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    Related Articles

    Amplification:

    Article Title: Androgen receptor expression predicts breast cancer survival: the role of genetic and epigenetic events
    Article Snippet: .. Bisulfite treated CpGenome™ Universal Methylated DNA (Chemicon, Millipore) and Whole genome amplified DNA prepared as per instructions with the GenomePlex® Complete Whole Genome Amplification kit (Sigma) were used as positive/methylated and negative/unmethylated controls, respectively. .. Triplicate PCR reactions were pooled and Shrimp Alkaline Phosphatase (Sequenom, San Diego) treatment performed followed by transcription and RNaseA Cleavage for the T-cleavage reaction.

    Whole Genome Amplification:

    Article Title: Androgen receptor expression predicts breast cancer survival: the role of genetic and epigenetic events
    Article Snippet: .. Bisulfite treated CpGenome™ Universal Methylated DNA (Chemicon, Millipore) and Whole genome amplified DNA prepared as per instructions with the GenomePlex® Complete Whole Genome Amplification kit (Sigma) were used as positive/methylated and negative/unmethylated controls, respectively. .. Triplicate PCR reactions were pooled and Shrimp Alkaline Phosphatase (Sequenom, San Diego) treatment performed followed by transcription and RNaseA Cleavage for the T-cleavage reaction.

    Positive Control:

    Article Title: Molecular Scoring of Hepatocellular Carcinoma for Predicting Metastatic Recurrence and Requirements of Systemic Chemotherapy
    Article Snippet: .. Bisulfite-treated CpGenome Universal Methylated DNA (CHEMICON International Inc., Temecula, CA, USA) was used as a positive control for methylated samples. .. We quantified methylation levels of three types of rDNAs, LINE-1 , Alu , and SAT2 because these methylation levels reportedly reflect the degree of global hypomethylation.

    Methylation:

    Article Title: Molecular Scoring of Hepatocellular Carcinoma for Predicting Metastatic Recurrence and Requirements of Systemic Chemotherapy
    Article Snippet: .. Bisulfite-treated CpGenome Universal Methylated DNA (CHEMICON International Inc., Temecula, CA, USA) was used as a positive control for methylated samples. .. We quantified methylation levels of three types of rDNAs, LINE-1 , Alu , and SAT2 because these methylation levels reportedly reflect the degree of global hypomethylation.

    Article Title: Frequency and Prognostic Impact of CEBPA Proximal, Distal and Core Promoter Methylation in Normal Karyotype AML: A Study on 623 Cases
    Article Snippet: .. Bisulfite treated CpGenome universal methylated DNA (Chemicon, Temecula, CA, USA) was used as a methylation-positive control. ..

    Article Title: Androgen receptor expression predicts breast cancer survival: the role of genetic and epigenetic events
    Article Snippet: .. Bisulfite treated CpGenome™ Universal Methylated DNA (Chemicon, Millipore) and DNA from T47D were used as positive/methylated and negative/unmethylated controls, respectively. .. The methylation status of these controls was confirmed by direct sequencing of MS-HRM products, purified using the QiaQuick Gel Extraction Kit (Qiagen), performed by the Australian Genome Research Facility (AGRF, Brisbane, Australia).

    Article Title: Molecular Scoring of Hepatocellular Carcinoma for Predicting Metastatic Recurrence and Requirements of Systemic Chemotherapy
    Article Snippet: .. A standard curve was generated using serial dilutions of bisulfite-treated CpGenome Universal Methylated DNA (Chemicon International Inc., Temecula, CA, USA). .. Methylation levels at each rDNAs sequence were normalized to those of CpG methylase-treated DNA.

    Article Title: Unique Association between Global DNA Hypomethylation and Chromosomal Alterations in Human Hepatocellular Carcinoma
    Article Snippet: .. A standard curve for each assay was generated from serial dilutions of the reference sample, bisulfite-treated CpGenome Universal Methylated DNA (CHEMICON International Inc., Temecula, CA). .. The methylation-independent consensus Alu sequence was used as an endogenous control, as described previously .

    Article Title: Androgen receptor expression predicts breast cancer survival: the role of genetic and epigenetic events
    Article Snippet: .. Bisulfite treated CpGenome™ Universal Methylated DNA (Chemicon, Millipore) and Whole genome amplified DNA prepared as per instructions with the GenomePlex® Complete Whole Genome Amplification kit (Sigma) were used as positive/methylated and negative/unmethylated controls, respectively. .. Triplicate PCR reactions were pooled and Shrimp Alkaline Phosphatase (Sequenom, San Diego) treatment performed followed by transcription and RNaseA Cleavage for the T-cleavage reaction.

    Article Title: Sensitive Detection of Colorectal Cancer in Peripheral Blood by Septin 9 DNA Methylation Assay
    Article Snippet: .. Each PCR run included a DNA Standard curve prepared using bisulfite treated CpGenome Universal Methylated DNA (Millipore (Chemicon), Billerica, MA) at concentrations between 50 pg/rxn–20 ng/rxn. .. Each run also included a no template control which was left uncapped throughout the process to control for contamination, and 3 no-template controls which were used to establish the baseline of the PCR reaction.

    Generated:

    Article Title: Molecular Scoring of Hepatocellular Carcinoma for Predicting Metastatic Recurrence and Requirements of Systemic Chemotherapy
    Article Snippet: .. A standard curve was generated using serial dilutions of bisulfite-treated CpGenome Universal Methylated DNA (Chemicon International Inc., Temecula, CA, USA). .. Methylation levels at each rDNAs sequence were normalized to those of CpG methylase-treated DNA.

    Article Title: Unique Association between Global DNA Hypomethylation and Chromosomal Alterations in Human Hepatocellular Carcinoma
    Article Snippet: .. A standard curve for each assay was generated from serial dilutions of the reference sample, bisulfite-treated CpGenome Universal Methylated DNA (CHEMICON International Inc., Temecula, CA). .. The methylation-independent consensus Alu sequence was used as an endogenous control, as described previously .

    Polymerase Chain Reaction:

    Article Title: Sensitive Detection of Colorectal Cancer in Peripheral Blood by Septin 9 DNA Methylation Assay
    Article Snippet: .. Each PCR run included a DNA Standard curve prepared using bisulfite treated CpGenome Universal Methylated DNA (Millipore (Chemicon), Billerica, MA) at concentrations between 50 pg/rxn–20 ng/rxn. .. Each run also included a no template control which was left uncapped throughout the process to control for contamination, and 3 no-template controls which were used to establish the baseline of the PCR reaction.

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  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
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  • 88
    Millipore bisulfite treated cpgenome universal methylated dna
    Bisulfite Treated Cpgenome Universal Methylated Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bisulfite treated cpgenome universal methylated dna/product/Millipore
    Average 88 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    bisulfite treated cpgenome universal methylated dna - by Bioz Stars, 2020-07
    88/100 stars
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