Structured Review

Zhongshan Golden Bridge Company biotinylated secondary antibody
Biotinylated Secondary Antibody, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated secondary antibody/product/Zhongshan Golden Bridge Company
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotinylated secondary antibody - by Bioz Stars, 2021-03
86/100 stars

Images

Related Articles

Incubation:

Article Title: Gene editing of the extra domain A positive fibronectin in various tumors, amplified the effects of CRISPR/Cas system on the inhibition of tumor progression
Article Snippet: Xenograft tumor tissues were sectioned into 4-μm-thick slices and stained with IST-9, anti-EDA+FN, anti-Ki-67, anti-CD34, and anti-E-cadherin (Abcam Ltd., Cambridge, MA, USA) antibodies at 4°C overnight. .. They were subsequently incubated with the biotinylated secondary antibody (1:200) for 1 h. The immunocomplexes were visualized with diaminobenzidine (Zhongshan Golden Bridge Biological Technology CO., LTD, Beijing, China). .. Following the IHC staining for EDA+FN, Ki-67, and E-cadherin, the obtained results were semi-quantitatively analyzed by histological score (H-score) based on the positively stained cell number and staining intensity.

Article Title: Marine fish peptides (collagen peptides) compound intake promotes wound healing in rats after cesarean section
Article Snippet: The sections were incubated with polyclonal rabbit anti-CTGF and anti-CD34 antibody (diluted 1:100, Abcam) at 4°C overnight. .. The sections were washed with phosphate-buffered saline and incubated with biotinylated secondary antibody for 30 min (diluted 1:1,000, Zhongshan Golden Bridge Inc., China). .. Sections were then treated with ABC solution at 37°C for 30 min and incubated with 3, 3-diaminobenzidine for 5 min. Counterstaining was carried out with Harris hematoxylin.

Article Title: Intrathecal decompression versus epidural decompression in the treatment of severe spinal cord injury in rat model: a randomized, controlled preclinical research
Article Snippet: After deparaffinization, hydrogen peroxide incubation, microwave antigen retrieval, and incubation in 2 % goat serum (SP9001 kit, Zhongshan Golden Bridge, Beijing, China), the sections were separately incubated in corresponding primary antibodies overnight at 4 °C. .. On the following day, the sections were incubated with a biotinylated secondary antibody at room temperature for 1 h and then incubated in avidin-biotin peroxidase complex (SP9001 kit, Zhongshan Golden Bridge, Beijing, China) for 30 min. .. The sections were visualized by 0.025 % diaminobenzidine (SP9001 kit, Zhongshan Golden Bridge, Beijing, China).

Article Title: Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells
Article Snippet: The blocking solution was removed, and primary antibodies to MAP-2, GFAP and NF-H were then added (each diluted 1:200) in PBS. .. Following incubation at 4°C overnight, biotinylated secondary antibody (15180A01; Zhongshan Golden Bridge Biotechnology, Co.) was added to the cells followed by further incubation at room temperature for 15 min, followed by the addition of horseradish peroxidase-conjugated streptavidin at room temperature for 15 min. Color development was performed by adding freshly prepared diaminobenzidine (DAB; Zhongshan Golden Bridge Biotechnology, Co.) for 1 min. After counterstaining with hematoxylin, the cells were rinsed repeatedly with water. .. Following the induction of differentiation for 6 h, the expression levels of the neuroal-specific proteins, MAP-2, NF-H and GFAP, were also detected by immunofluorescence staining as follows: the medium from the culture plates was discarded, and the cells were rinsed once gently with PBS and fixed with 4% paraformaldehyde for 20 min at room temperature.

Article Title: Involvement of Activated Cdc42 Kinase1 in Colitis and Colorectal Neoplasms
Article Snippet: The slides were incubated with a rabbit polyclonal antibody against human ACK1 (sc-323, 1: 100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4°C overnight. .. After washing with phosphate-buffered saline (PBS)/Tween 3 times for 10 min, the slides were incubated with a 1: 500 dilution of the biotinylated secondary antibody (Zhongshan Goldenbridge Biotechnology Co., Ltd., Beijing, China) for 30 min. After another washing with PBS for 3 times, they were incubated for 1 min with the avidin-biotin complex reagent at room temperature. .. The colorimetric reaction was performed with 3, 3′-diaminobenzidine (Beyotime Institute of Biotechnology Co., Ltd., Haimen, Jiangsu, China) as the chromogen and nickel chloride enhancement.

Article Title: Post-Transcriptional and Epigenetic Regulation of Antigen Processing Machinery (APM) Components and HLA-I in Cervical Cancers from Uighur Women
Article Snippet: After cooling and rinsing in distilled water, endogenous peroxidase activity was blocked by incubating sections for 15 min in 3% H2 O2 , followed by rinsing in 0.01 M PBS (pH 7.4) for 10 min. .. Samples were preincubated with a protein blocking solution for 10 min and the sections were incubated at 4°C overnight in a humid chamber with the indicated antibodies, Slides were washed three times in PBS and then incubated with a biotinylated secondary antibody (Zhong Shan Goldenbridge Biotechnology Co. Ltd., China) for 15 min at room temperature. .. The reaction products were visualized with diaminobenzidine (DAB Kit; Zhongshan Goldenbridge Biotechnology).

Article Title: Bevacizumab or fibronectin gene editing inhibits the osteoclastogenic effects of fibroblasts derived from human radicular cysts
Article Snippet: Phosphate-buffered saline (PBS) was used as the negative control. .. The slices were subsequently incubated with a biotinylated secondary antibody (1:200) for 1 h. Immunocomplexes were visualized with diaminobenzidine (Zhongshan Golden Bridge Biological Technology Co., Ltd., Beijing, China). .. As described in a previous study [ ], EDA+ FN staining in the stroma was quantified using a microscope (Olympus, Tokyo, Japan) and the Image-Pro Plus ver.

Avidin-Biotin Assay:

Article Title: Intrathecal decompression versus epidural decompression in the treatment of severe spinal cord injury in rat model: a randomized, controlled preclinical research
Article Snippet: After deparaffinization, hydrogen peroxide incubation, microwave antigen retrieval, and incubation in 2 % goat serum (SP9001 kit, Zhongshan Golden Bridge, Beijing, China), the sections were separately incubated in corresponding primary antibodies overnight at 4 °C. .. On the following day, the sections were incubated with a biotinylated secondary antibody at room temperature for 1 h and then incubated in avidin-biotin peroxidase complex (SP9001 kit, Zhongshan Golden Bridge, Beijing, China) for 30 min. .. The sections were visualized by 0.025 % diaminobenzidine (SP9001 kit, Zhongshan Golden Bridge, Beijing, China).

Article Title: Involvement of Activated Cdc42 Kinase1 in Colitis and Colorectal Neoplasms
Article Snippet: The slides were incubated with a rabbit polyclonal antibody against human ACK1 (sc-323, 1: 100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4°C overnight. .. After washing with phosphate-buffered saline (PBS)/Tween 3 times for 10 min, the slides were incubated with a 1: 500 dilution of the biotinylated secondary antibody (Zhongshan Goldenbridge Biotechnology Co., Ltd., Beijing, China) for 30 min. After another washing with PBS for 3 times, they were incubated for 1 min with the avidin-biotin complex reagent at room temperature. .. The colorimetric reaction was performed with 3, 3′-diaminobenzidine (Beyotime Institute of Biotechnology Co., Ltd., Haimen, Jiangsu, China) as the chromogen and nickel chloride enhancement.

Blocking Assay:

Article Title: Post-Transcriptional and Epigenetic Regulation of Antigen Processing Machinery (APM) Components and HLA-I in Cervical Cancers from Uighur Women
Article Snippet: After cooling and rinsing in distilled water, endogenous peroxidase activity was blocked by incubating sections for 15 min in 3% H2 O2 , followed by rinsing in 0.01 M PBS (pH 7.4) for 10 min. .. Samples were preincubated with a protein blocking solution for 10 min and the sections were incubated at 4°C overnight in a humid chamber with the indicated antibodies, Slides were washed three times in PBS and then incubated with a biotinylated secondary antibody (Zhong Shan Goldenbridge Biotechnology Co. Ltd., China) for 15 min at room temperature. .. The reaction products were visualized with diaminobenzidine (DAB Kit; Zhongshan Goldenbridge Biotechnology).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Zhongshan Golden Bridge Company biotinylated goat anti rabbit igg secondary antibody
    Biotinylated Goat Anti Rabbit Igg Secondary Antibody, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit igg secondary antibody/product/Zhongshan Golden Bridge Company
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit igg secondary antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Zhongshan Golden Bridge Company biotinylated secondary antibodies
    Biotinylated Secondary Antibodies, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Zhongshan Golden Bridge Company
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Zhongshan Golden Bridge Company biotinylated secondary antibody
    Biotinylated Secondary Antibody, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Zhongshan Golden Bridge Company
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Zhongshan Golden Bridge Company biotinylated anti rabbit secondary antibody
    Biotinylated Anti Rabbit Secondary Antibody, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit secondary antibody/product/Zhongshan Golden Bridge Company
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit secondary antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results