biotinylated secondary antibody  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    Lab Vision biotinylated Goat anti Polyvalent Ready To Use
    Description:
    Employ a labeled streptavidin biotin immunoenzymatic antigen detection system with the Thermo Scientific Lab Vision Biotinylated Goat anti Polyvalent Ready To Use
    Catalog Number:
    tp-060-bn
    Price:
    None
    Category:
    Lab Reagents and Chemicals
    Applications:
    Anatomical Pathology|Clinical
    Buy from Supplier


    Structured Review

    Thermo Fisher biotinylated secondary antibody
    The ELISA-Array print pattern. The 6×6 array was printed in each well of a 96-well plate. The outer edges contain fiducial spots of <t>biotinylated</t> anti-kappa antibody for orientation (#11). All other spots are printed in triplicate, using anti-IgG Fc antibody as a positive control (#1) and GFP-foldon as a negative control (#10). In the top half of the pattern are Influenza A HA group I proteins (#2-4) and the 2018 vaccine (#5), and in the bottom half are Influenza A HA group II proteins (#6-7) and Influenza B HA proteins (#8-9). At right is an image of one array well developed after binding reference plasma on H1N1 A/Michigan/45/2015 HA trimer.
    Employ a labeled streptavidin biotin immunoenzymatic antigen detection system with the Thermo Scientific Lab Vision Biotinylated Goat anti Polyvalent Ready To Use
    https://www.bioz.com/result/biotinylated secondary antibody/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Adaption of a Conventional ELISA to a 96-well ELISA-Array for Measuring the Antibody Responses to Influenza virus proteins, viruses and vaccines"

    Article Title: Adaption of a Conventional ELISA to a 96-well ELISA-Array for Measuring the Antibody Responses to Influenza virus proteins, viruses and vaccines

    Journal: bioRxiv

    doi: 10.1101/2019.12.20.885285

    The ELISA-Array print pattern. The 6×6 array was printed in each well of a 96-well plate. The outer edges contain fiducial spots of biotinylated anti-kappa antibody for orientation (#11). All other spots are printed in triplicate, using anti-IgG Fc antibody as a positive control (#1) and GFP-foldon as a negative control (#10). In the top half of the pattern are Influenza A HA group I proteins (#2-4) and the 2018 vaccine (#5), and in the bottom half are Influenza A HA group II proteins (#6-7) and Influenza B HA proteins (#8-9). At right is an image of one array well developed after binding reference plasma on H1N1 A/Michigan/45/2015 HA trimer.
    Figure Legend Snippet: The ELISA-Array print pattern. The 6×6 array was printed in each well of a 96-well plate. The outer edges contain fiducial spots of biotinylated anti-kappa antibody for orientation (#11). All other spots are printed in triplicate, using anti-IgG Fc antibody as a positive control (#1) and GFP-foldon as a negative control (#10). In the top half of the pattern are Influenza A HA group I proteins (#2-4) and the 2018 vaccine (#5), and in the bottom half are Influenza A HA group II proteins (#6-7) and Influenza B HA proteins (#8-9). At right is an image of one array well developed after binding reference plasma on H1N1 A/Michigan/45/2015 HA trimer.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Positive Control, Negative Control, Binding Assay

    Related Articles

    Binding Assay:

    Article Title: Lck Domains Differentially Contribute to Pre-T Cell Receptor (Tcr)-And TCR-?/?-Regulated Developmental Transitions
    Article Snippet: Thymocytes were stained for surface expression of CD4, CD8, and CD3 molecules as described previously , using PE-conjugated anti-CD4 (CT-CD4), FITC-conjugated anti-CD8 (CT-CD8α), and biotinylated anti-CD3ε (500.A2; all from Caltag Laboratories). .. Binding of biotinylated antibodies was detected using streptavidin-TriColor conjugate (Caltag Laboratories). .. Each analysis was performed on 2 × 104 events acquired in list mode using a FACSort™ flow cytometer, and was analyzed using CELLQuest™ software (Becton Dickinson).

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: The expression of class II MHC, B7.2, and CD25 was analyzed on resting and anti-IgM– or anti-CD40–treated splenic B cells were stimulated for 24 h. Cells were surface stained with biotinylated monoclonal antibodies M5/114 (anti-class II MHC), GL-1 (anti-B7.2), PC16 (anti–IL-2 receptor α chain CD25), or isotype-matched control monoclonal antibodies as described previously ( ). .. Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent. .. Viable (PI negative) cells (5,000–10,000) were analyzed in a FACScan® flow cytometer ( Becton Dickinson ).

    Incubation:

    Article Title: MIA PaCa-2 and PANC-1 – pancreas ductal adenocarcinoma cell lines with neuroendocrine differentiation and somatostatin receptors
    Article Snippet: Primary antibodies against CK5.6 (clone D5/16B4; Cell Marque, California, USA), at a dilution of 1:50 for 40 minutes, with skin cells as positive control, were applied to the sections and incubated at room temperature, as were ready-to-use AE1/AE3 (clone AE1/AE3; Novocastra Laboratories, Ltd., Newcastle, UK), for 30 minutes, with appendix cells as positive control; E-cadherin (clone 36B5; Novocastra Laboratories Ltd., Newcastle, UK), at a dilution of 1:100 for 30 minutes, with tonsil cells as positive control; vimentin (clone VIM 3B4; DakoCytomation, Glostrup, Denmark), at a dilution of 1:200 for 30 minutes, with colon cells as positive control; chromogranin A (clone DAK-A3; DakoCytomation, Glostrup, Denmark), at a dilution of 1:300 for 30 minutes, with pancreas cells as positive control; CD56 (clone 123C3; DakoCytomation, Glostrup, Denmark), at a dilution of 1:100 for 44 minutes, with colon cells as positive control; synaptophysin (polyclonal; Thermo Scientific, MA, USA), at a dilution of 1:50 for 36 minutes, with thyroid cell as positive control; SSTR2 (polyclonal; abcam (ab9550), Cambridge, United Kingdom), at a dilution of 1:300 for 30 minutes, with kidney cells as positive control; and NTR1 (polyclonal H-130; Santa Cruz Biotechnology (sc-15311), California, United States), at a dilution of 1:250 for 30 minutes, with a pancreatic xenographt as positive control. .. They were washed with phosphate-buffered saline (PBS) (TP-125-PB; Lab Vision Corporation; Fremont CA; USA), after which slides were incubated with biotin-labeled secondary antibody, biotynilated goat anti-polyvalent (TP-125-BN; Lab Vision Corporation; Fremont CA; USA), for 15 minutes. .. Primary antibody binding was localized in tissues using peroxidase-conjugated streptavidin (TP-125-HR; Lab Vision Corporation; Fremont CA; USA), and 3,3′-diaminobenzidine tetrahydrochloride (DAB) (RE7190-K; Novocastra Laboratories Ltd., Newcastle, UK) was used as chromogen, according to the manufacturer’s instructions.

    Article Title: Proangiogenic effects of soluble α-Klotho on systemic sclerosis dermal microvascular endothelial cells
    Article Snippet: After blocking non-specific site binding, slides were incubated overnight at 4 °C with rabbit monoclonal anti-human α-Klotho antibody (1:20 dilution; catalog number ab181373; Abcam) diluted in 1% bovine serum albumin (BSA) in PBS. .. The day after, tissue sections were washed three times in PBS and incubated with biotinylated secondary antibodies (UltraVision Large Volume Detection System Anti-Polyvalent, HRP; LabVision) for 10 minutes at room temperature. .. Subsequently, the slides were washed three times in PBS and incubated with streptavidin peroxidase (UltraVision Large Volume Detection System Anti-Polyvalent, HRP; LabVision) for 10 minutes at room temperature.

    Article Title: Proangiogenic effects of soluble α-Klotho on systemic sclerosis dermal microvascular endothelial cells
    Article Snippet: Slides were then washed, treated with 3% H2 O2 in PBS for 15 minutes at room temperature and subsequently blocked with Ultra V block (UltraVision Large Volume Detection System Anti-Polyvalent, HRP; LabVision) for 10 minutes. .. Cells were incubated overnight at 4 °C with rabbit monoclonal anti-human α-Klotho antibody (catalog number ab181373; Abcam) at 1:20 dilution in 1% BSA in PBS, followed by incubation with biotinylated secondary antibodies and streptavidin peroxidase (UltraVision Large Volume Detection System Anti-Polyvalent, HRP; LabVision) at room temperature. .. Immunoreactivity was developed with 3-amino-9-ethylcarbazole (AEC kit; LabVision).

    Staining:

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: The expression of class II MHC, B7.2, and CD25 was analyzed on resting and anti-IgM– or anti-CD40–treated splenic B cells were stimulated for 24 h. Cells were surface stained with biotinylated monoclonal antibodies M5/114 (anti-class II MHC), GL-1 (anti-B7.2), PC16 (anti–IL-2 receptor α chain CD25), or isotype-matched control monoclonal antibodies as described previously ( ). .. Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent. .. Viable (PI negative) cells (5,000–10,000) were analyzed in a FACScan® flow cytometer ( Becton Dickinson ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Thermo Fisher alexa fluor 488
    Endocytosis of NogoΔ20 is required for NogoΔ20-induced growth cone collapse in hippocampal neurons. (A and B) Morphology of noncollapsed (A, arrow) and collapsed growth cones (B, arrowhead) of E19 hippocampal neurons at DIV 4 was visualized by staining of F-actin with <t>phalloidin–Alexa</t> <t>Fluor</t> 488 (green). (C) Quantification of the proportion of collapsed growth cones after incubation with 300 nM NogoΔ20 (shaded bars) or with 300 nM NogoΔ21 (open bars). (D and E) The morphology of hippocampal neurons infected with immunodeficient recombinant adenovirus containing HA-tagged wt Pincher (D, red) or HA-tagged dn PincherG68E (E) upon treatment for 30 min with 300 nM NogoΔ20 was visualized with phalloidin (green). (F) Most growth cones remained uncollapsed when HA-tagged dn PincherG68E was overexpressed (open bars) compared with wt Pincher (shaded bars). (G and H) Growth cone morphology of hippocampal neurons upon treatment with 40 nM semaphorin 3A overexpressing either wt HA-Pincher protein (G) or dn HA-PincherG68E protein (H). (I) Treatment with 40 nM semaphorin 3A for 30 min leads to growth cone collapse in the presence of both wt HA-Pincher (shaded bars) and dn HA-Pincherg68E (open bars). Data represent the mean of three (semaphorin 3A) or four (NogoΔ20) independent experiments ± SEM (90 neurons per group and experiment). Asterisks mark highly significant differences between wt and dn Pincher-infected hippocampal neurons (***, P
    Alexa Fluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 488/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 488 - by Bioz Stars, 2021-03
    98/100 stars
      Buy from Supplier

    92
    Thermo Fisher phospho ve cadherin
    Overexpression of PAFAH2 represses PM-induced EC barrier dysfunction. ( A, B, C ) Cells were transfected with plasmid encoding human PAFAH2 or control empty vector for 24 h followed by treatment with PM (20 μg/cm 2 , 4 hr). Cell lysates were analyzed by Western blotting to determine <t>phospho-VE-cadherin</t> ( A ), membrane bound VE-cadherin ( B ) and biotinylated VE-cadherin ( C ) levels. Detection of VE-cadherin in total cell lysates was used as normalization control. ( D ) Cells transfected with empty control vector or PAFAH2 overexpressing plasmid were treated with PM and TER was monitored over time. Data are expressed as mean ± S.D.; n = 4.
    Phospho Ve Cadherin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ve cadherin/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho ve cadherin - by Bioz Stars, 2021-03
    92/100 stars
      Buy from Supplier

    86
    Thermo Fisher avidin alexa 594
    Endogenous ARHGAP4 protein is present in the peripheral zone of NIH/3T3 cells and growth cones ) and a mouse monoclonal antibody against β-tubulin III to label axons and the central region of growth cones. Fluorescence was visualized using biotinylated secondary antibodies labeled with avidin-Alexa 594 (red) and avidin-Alexa 488 (green). Images were viewed using a Leica confocal laser scanning microscope. Yellow indicates regions of overlapping immunoreactivity. Panels A–C: NIH/3T3 cells, Panels D–F: MF growth cones. The leading edge of the NIH/3T3 cell and the distal tips of filopodia of dentate granule cells are indicated by arrows in these representative images (n =3). Calibration = 5μm.
    Avidin Alexa 594, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin alexa 594/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin alexa 594 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Endocytosis of NogoΔ20 is required for NogoΔ20-induced growth cone collapse in hippocampal neurons. (A and B) Morphology of noncollapsed (A, arrow) and collapsed growth cones (B, arrowhead) of E19 hippocampal neurons at DIV 4 was visualized by staining of F-actin with phalloidin–Alexa Fluor 488 (green). (C) Quantification of the proportion of collapsed growth cones after incubation with 300 nM NogoΔ20 (shaded bars) or with 300 nM NogoΔ21 (open bars). (D and E) The morphology of hippocampal neurons infected with immunodeficient recombinant adenovirus containing HA-tagged wt Pincher (D, red) or HA-tagged dn PincherG68E (E) upon treatment for 30 min with 300 nM NogoΔ20 was visualized with phalloidin (green). (F) Most growth cones remained uncollapsed when HA-tagged dn PincherG68E was overexpressed (open bars) compared with wt Pincher (shaded bars). (G and H) Growth cone morphology of hippocampal neurons upon treatment with 40 nM semaphorin 3A overexpressing either wt HA-Pincher protein (G) or dn HA-PincherG68E protein (H). (I) Treatment with 40 nM semaphorin 3A for 30 min leads to growth cone collapse in the presence of both wt HA-Pincher (shaded bars) and dn HA-Pincherg68E (open bars). Data represent the mean of three (semaphorin 3A) or four (NogoΔ20) independent experiments ± SEM (90 neurons per group and experiment). Asterisks mark highly significant differences between wt and dn Pincher-infected hippocampal neurons (***, P

    Journal: The Journal of Cell Biology

    Article Title: Pincher-generated Nogo-A endosomes mediate growth cone collapse and retrograde signaling

    doi: 10.1083/jcb.200906089

    Figure Lengend Snippet: Endocytosis of NogoΔ20 is required for NogoΔ20-induced growth cone collapse in hippocampal neurons. (A and B) Morphology of noncollapsed (A, arrow) and collapsed growth cones (B, arrowhead) of E19 hippocampal neurons at DIV 4 was visualized by staining of F-actin with phalloidin–Alexa Fluor 488 (green). (C) Quantification of the proportion of collapsed growth cones after incubation with 300 nM NogoΔ20 (shaded bars) or with 300 nM NogoΔ21 (open bars). (D and E) The morphology of hippocampal neurons infected with immunodeficient recombinant adenovirus containing HA-tagged wt Pincher (D, red) or HA-tagged dn PincherG68E (E) upon treatment for 30 min with 300 nM NogoΔ20 was visualized with phalloidin (green). (F) Most growth cones remained uncollapsed when HA-tagged dn PincherG68E was overexpressed (open bars) compared with wt Pincher (shaded bars). (G and H) Growth cone morphology of hippocampal neurons upon treatment with 40 nM semaphorin 3A overexpressing either wt HA-Pincher protein (G) or dn HA-PincherG68E protein (H). (I) Treatment with 40 nM semaphorin 3A for 30 min leads to growth cone collapse in the presence of both wt HA-Pincher (shaded bars) and dn HA-Pincherg68E (open bars). Data represent the mean of three (semaphorin 3A) or four (NogoΔ20) independent experiments ± SEM (90 neurons per group and experiment). Asterisks mark highly significant differences between wt and dn Pincher-infected hippocampal neurons (***, P

    Article Snippet: Secondary antibodies used were Alexa Fluor 488, Alexa Fluor 594, avidin-rhodamin, and avidin-FITC (all from Invitrogen).

    Techniques: Staining, Incubation, Infection, Recombinant

    Overexpression of PAFAH2 represses PM-induced EC barrier dysfunction. ( A, B, C ) Cells were transfected with plasmid encoding human PAFAH2 or control empty vector for 24 h followed by treatment with PM (20 μg/cm 2 , 4 hr). Cell lysates were analyzed by Western blotting to determine phospho-VE-cadherin ( A ), membrane bound VE-cadherin ( B ) and biotinylated VE-cadherin ( C ) levels. Detection of VE-cadherin in total cell lysates was used as normalization control. ( D ) Cells transfected with empty control vector or PAFAH2 overexpressing plasmid were treated with PM and TER was monitored over time. Data are expressed as mean ± S.D.; n = 4.

    Journal: PLoS ONE

    Article Title: Role of truncated oxidized phospholipids in acute endothelial barrier dysfunction caused by particulate matter

    doi: 10.1371/journal.pone.0206251

    Figure Lengend Snippet: Overexpression of PAFAH2 represses PM-induced EC barrier dysfunction. ( A, B, C ) Cells were transfected with plasmid encoding human PAFAH2 or control empty vector for 24 h followed by treatment with PM (20 μg/cm 2 , 4 hr). Cell lysates were analyzed by Western blotting to determine phospho-VE-cadherin ( A ), membrane bound VE-cadherin ( B ) and biotinylated VE-cadherin ( C ) levels. Detection of VE-cadherin in total cell lysates was used as normalization control. ( D ) Cells transfected with empty control vector or PAFAH2 overexpressing plasmid were treated with PM and TER was monitored over time. Data are expressed as mean ± S.D.; n = 4.

    Article Snippet: Antibodies to phospho-VE-cadherin (pTyr-658 and pTyr-731) were obtained from Invitrogen (Carlsbad, CA) and VE-cadherin antibody was from Santa Cruz Biotechnology (San Jose, CA). p120-Catenin antibody was from BD biosciences (San Diego, CA) and HRP-linked anti-mouse and anti-rabbit IgG were obtained from Cell Signaling (Beverly, MA).

    Techniques: Over Expression, Transfection, Plasmid Preparation, Western Blot

    Tr-OxPLs augment PM-induced AJ disruption. ( A ) Cells were treated with PM for indicated time periods, and phospho-VE-cadherin (Tyr 658 and Tyr 731) levels were determined by Western blotting. ( B ) Cells were treated with increasing concentrations of POVPC for 30 min, and cell lysates were analyzed by Western blotting to detect phospho-VE-cadherin. ( C, D ) Cells were challenged with low dose of POVPC (10 μg/mL) alone or in combination with PM, and phospho-VE-cadherin levels ( C ) or biotinylated VE-cadherin levels ( D ) were detected by Western blotting. The total cell lysates were used as normalization control. ( E ) VE-cadherin staining of pulmonary EC following the treatment with PM alone or in combination with POVPC.

    Journal: PLoS ONE

    Article Title: Role of truncated oxidized phospholipids in acute endothelial barrier dysfunction caused by particulate matter

    doi: 10.1371/journal.pone.0206251

    Figure Lengend Snippet: Tr-OxPLs augment PM-induced AJ disruption. ( A ) Cells were treated with PM for indicated time periods, and phospho-VE-cadherin (Tyr 658 and Tyr 731) levels were determined by Western blotting. ( B ) Cells were treated with increasing concentrations of POVPC for 30 min, and cell lysates were analyzed by Western blotting to detect phospho-VE-cadherin. ( C, D ) Cells were challenged with low dose of POVPC (10 μg/mL) alone or in combination with PM, and phospho-VE-cadherin levels ( C ) or biotinylated VE-cadherin levels ( D ) were detected by Western blotting. The total cell lysates were used as normalization control. ( E ) VE-cadherin staining of pulmonary EC following the treatment with PM alone or in combination with POVPC.

    Article Snippet: Antibodies to phospho-VE-cadherin (pTyr-658 and pTyr-731) were obtained from Invitrogen (Carlsbad, CA) and VE-cadherin antibody was from Santa Cruz Biotechnology (San Jose, CA). p120-Catenin antibody was from BD biosciences (San Diego, CA) and HRP-linked anti-mouse and anti-rabbit IgG were obtained from Cell Signaling (Beverly, MA).

    Techniques: Western Blot, Staining

    PM induces AJ breakdown. ( A ) Cells were treated with PM (20 μg/cm 2 , 1h) and cell junctions were visualized by immunostaining with VE-cadherin antibody. ( B ) Surface protein biotinylation assay was performed as described in Methods and lysates were run on Western blot to detect biotinylated and total VE-cadherin. ( C ) Membrane fractions isolated by sub-cellular protein fractionations were subjected for Western blotting against p120-catenin and VE-cadherin antibodies. The total cell lysates were used as normalization control.

    Journal: PLoS ONE

    Article Title: Role of truncated oxidized phospholipids in acute endothelial barrier dysfunction caused by particulate matter

    doi: 10.1371/journal.pone.0206251

    Figure Lengend Snippet: PM induces AJ breakdown. ( A ) Cells were treated with PM (20 μg/cm 2 , 1h) and cell junctions were visualized by immunostaining with VE-cadherin antibody. ( B ) Surface protein biotinylation assay was performed as described in Methods and lysates were run on Western blot to detect biotinylated and total VE-cadherin. ( C ) Membrane fractions isolated by sub-cellular protein fractionations were subjected for Western blotting against p120-catenin and VE-cadherin antibodies. The total cell lysates were used as normalization control.

    Article Snippet: Antibodies to phospho-VE-cadherin (pTyr-658 and pTyr-731) were obtained from Invitrogen (Carlsbad, CA) and VE-cadherin antibody was from Santa Cruz Biotechnology (San Jose, CA). p120-Catenin antibody was from BD biosciences (San Diego, CA) and HRP-linked anti-mouse and anti-rabbit IgG were obtained from Cell Signaling (Beverly, MA).

    Techniques: Immunostaining, Cell Surface Biotinylation Assay, Western Blot, Isolation

    Endogenous ARHGAP4 protein is present in the peripheral zone of NIH/3T3 cells and growth cones ) and a mouse monoclonal antibody against β-tubulin III to label axons and the central region of growth cones. Fluorescence was visualized using biotinylated secondary antibodies labeled with avidin-Alexa 594 (red) and avidin-Alexa 488 (green). Images were viewed using a Leica confocal laser scanning microscope. Yellow indicates regions of overlapping immunoreactivity. Panels A–C: NIH/3T3 cells, Panels D–F: MF growth cones. The leading edge of the NIH/3T3 cell and the distal tips of filopodia of dentate granule cells are indicated by arrows in these representative images (n =3). Calibration = 5μm.

    Journal: Molecular and cellular neurosciences

    Article Title: ARHGAP4 IS A NOVEL RHOGAP THAT MEDIATES INHIBITION OF CELL MOTILITY AND AXON OUTGROWTH

    doi: 10.1016/j.mcn.2007.07.004

    Figure Lengend Snippet: Endogenous ARHGAP4 protein is present in the peripheral zone of NIH/3T3 cells and growth cones ) and a mouse monoclonal antibody against β-tubulin III to label axons and the central region of growth cones. Fluorescence was visualized using biotinylated secondary antibodies labeled with avidin-Alexa 594 (red) and avidin-Alexa 488 (green). Images were viewed using a Leica confocal laser scanning microscope. Yellow indicates regions of overlapping immunoreactivity. Panels A–C: NIH/3T3 cells, Panels D–F: MF growth cones. The leading edge of the NIH/3T3 cell and the distal tips of filopodia of dentate granule cells are indicated by arrows in these representative images (n =3). Calibration = 5μm.

    Article Snippet: The secondary antibody was complexed with avidin alexa 594 (Molecular Probes) to visualize immunofluorescence.

    Techniques: Fluorescence, Labeling, Avidin-Biotin Assay, Laser-Scanning Microscopy