biotinylated rabbit anti mouse igg  (Millipore)


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    Structured Review

    Millipore biotinylated rabbit anti mouse igg
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Rabbit Anti Mouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated rabbit anti mouse igg/product/Millipore
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biotinylated rabbit anti mouse igg - by Bioz Stars, 2020-08
    91/100 stars

    Images

    1) Product Images from "Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins"

    Article Title: Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

    Journal: Frontiers in Chemistry

    doi: 10.3389/fchem.2019.00031

    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the biotinylated detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse IgG; Neg.Co. = BSA.
    Figure Legend Snippet: Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the biotinylated detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse IgG; Neg.Co. = BSA.

    Techniques Used: Labeling, Amplification, Generated, Flow Cytometry

    Related Articles

    Microscopy:

    Article Title: Improvement of non-alcoholic steatohepatitis by hepatocyte-like cells generated from iPSCs with Oct4/Sox2/Klf4/Parp1
    Article Snippet: .. Immunoreactive signals were detected with a mixture of biotinylated rabbit anti-mouse IgG and Fluoresave (Calbiochem, La Jolla, CA, USA) and a confocal microscope (Olympus, FV300). .. Microarray analysis and bioinformatics Total RNA was extracted from cells using Trizol reagent (Life Technologies, Bethesda, MD, USA) and the Qiagen RNAeasy (Qiagen, Valencia, CA, USA) column for purification.

    Article Title: Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells without Reprogramming Factor c-Myc
    Article Snippet: .. Immunoreactive signals were detected with a mixture of biotinylated rabbit anti-mouse IgG and Fluoresave (Calbiochem, La Jolla, CA, USA) and a confocal microscope (Olympus, FV300). ..

    Negative Control:

    Article Title: Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins
    Article Snippet: .. Biotinylated rabbit anti-mouse IgG (Sigma-Aldrich GmbH, Taufkirchen, Germany) was used as positive control (2.5 μg/mL in 0.02% (w/v) BSA/PBS) and 400 μg/mL BSA/PBS as negative control. .. After spotting, the biochips were incubated in a humidity chamber with PBS for 2 h at room temperature, followed by a 16 h-incubation at 4°C.

    other:

    Article Title: Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions
    Article Snippet: Antibodies The rabbit polyclonal antibody against PCX, mouse monoclonal antibody against nestin, biotinylated sheep anti-rabbit IgG and biotinylated rabbit anti-mouse IgG were purchased from Sigma (St. Louis, MO, USA).

    Article Title: Human podocyte injury in the early course of hypertensive renal injury
    Article Snippet: The secondary antibodies used were: biotinylated sheep anti-rabbit IgG and biotinylated rabbit anti-mouse IgG (Sigma, St. Louis, MO, United States), tetramethylrhodamine (TRITC)-labeled anti-rabbit IgG and fluorescein isothiocyanate (FITC)-labeled anti-mouse IgG (Abcam, Cambridge, MA, United States).

    Incubation:

    Article Title: Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with vaccine candidate F18ac+ nonenterotoxigenic E. coli strain
    Article Snippet: .. Mouse anti-swine CD antigen-specific mAbs ( ) were added over the sections and incubated for 1 hour at room temperature.The secondary antibody, biotinylated rabbit anti-mouse IgG (Sigma, St. Louis, USA) diluted 1:500 in PBS was applied to cover the tissue sections and incubated at room temperature for 1 hour. ..

    Article Title: Tamoxifen induction of angiogenic factor expression in endometrium
    Article Snippet: .. Again the sections were rinsed for 2×5 min, followed by a 30 min incubation with the secondary biotinylated rabbit anti mouse IgG (Sigma), diluted 1 : 400. .. The sections were incubated with phosphate conjugated streptavidin at a dilution of 1 : 200 for 30 min.

    Positive Control:

    Article Title: Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins
    Article Snippet: .. Biotinylated rabbit anti-mouse IgG (Sigma-Aldrich GmbH, Taufkirchen, Germany) was used as positive control (2.5 μg/mL in 0.02% (w/v) BSA/PBS) and 400 μg/mL BSA/PBS as negative control. .. After spotting, the biochips were incubated in a humidity chamber with PBS for 2 h at room temperature, followed by a 16 h-incubation at 4°C.

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    Millipore biotinylated rabbit anti mouse igg
    Biotinylated Rabbit Anti Mouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated rabbit anti mouse igg/product/Millipore
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    https://www.bioz.com/result/biotinylated secondary goat anti mouse igg goat anti rabbit igg/product/Millipore
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