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R&D Systems various cytokine protein levels
Serum <t>cytokine</t> levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p
Various Cytokine Protein Levels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 9078 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 9078 article reviews
Price from $9.99 to $1999.99
various cytokine protein levels - by Bioz Stars, 2020-09
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1) Product Images from "In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses"

Article Title: In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses

Journal: BMC Veterinary Research

doi: 10.1186/s12917-018-1675-x

Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p
Figure Legend Snippet: Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

mRNA transcription of cytokines in PBMCs collected from pigs infected with the VR2332c and JA142c PRRSV strains at 0, 7, 14, 21 and 28 dpc. Analysis of mRNA transcription in PBMCs (n = 5) collected from infected and control pigs using real-time PCR. Asterisks indicate significant differences in cytokine expression induced by each virus compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05)
Figure Legend Snippet: mRNA transcription of cytokines in PBMCs collected from pigs infected with the VR2332c and JA142c PRRSV strains at 0, 7, 14, 21 and 28 dpc. Analysis of mRNA transcription in PBMCs (n = 5) collected from infected and control pigs using real-time PCR. Asterisks indicate significant differences in cytokine expression induced by each virus compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05)

Techniques Used: Infection, Real-time Polymerase Chain Reaction, Expressing

Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

2) Product Images from "In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses"

Article Title: In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses

Journal: BMC Veterinary Research

doi: 10.1186/s12917-018-1675-x

Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p
Figure Legend Snippet: Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

mRNA transcription of cytokines in PBMCs collected from pigs infected with the VR2332c and JA142c PRRSV strains at 0, 7, 14, 21 and 28 dpc. Analysis of mRNA transcription in PBMCs (n = 5) collected from infected and control pigs using real-time PCR. Asterisks indicate significant differences in cytokine expression induced by each virus compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05)
Figure Legend Snippet: mRNA transcription of cytokines in PBMCs collected from pigs infected with the VR2332c and JA142c PRRSV strains at 0, 7, 14, 21 and 28 dpc. Analysis of mRNA transcription in PBMCs (n = 5) collected from infected and control pigs using real-time PCR. Asterisks indicate significant differences in cytokine expression induced by each virus compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05)

Techniques Used: Infection, Real-time Polymerase Chain Reaction, Expressing

Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

3) Product Images from "Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine"

Article Title: Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine

Journal: Porcine Health Management

doi: 10.1186/s40813-018-0112-7

Evolution of IFN-α levels (Limit of detection: 3.9 pg/mL)
Figure Legend Snippet: Evolution of IFN-α levels (Limit of detection: 3.9 pg/mL)

Techniques Used:

4) Product Images from "Partial Activation of Natural Killer and γδ T Cells by Classical Swine Fever Viruses Is Associated with Type I Interferon Elicited from Plasmacytoid Dendritic Cells"

Article Title: Partial Activation of Natural Killer and γδ T Cells by Classical Swine Fever Viruses Is Associated with Type I Interferon Elicited from Plasmacytoid Dendritic Cells

Journal: Clinical and Vaccine Immunology : CVI

doi: 10.1128/CVI.00382-14

Investigation of CSFV-infected pDC-derived cytokines on the MHC class II expression of NK and γδ T cells. (A) pDCs or mDCs were exposed to CSFV C-strain, Brescia strain, or a mock virus-infected cryolysate supernatant, and after 24 h in culture, the levels of TNF-α and type I IFN in the supernatants were analyzed using a porcine cytokine multiplex ELISA and Mx/CAT reporter gene assay, respectively. (B) Enriched NK and γδ T cells were then cultured in cRPMI medium alone or in the presence of porcine recombinant IFN-α and/or TNF-α, at concentrations comparable to those detected in infected pDC cocultures, and the expression of MHC-II was assessed by flow cytometry after 24 h. The mean ± SEM data from three independent experiments utilizing different animals are shown. The values for each group were compared to the corresponding unstimulated control (mock stimulation in panel A and no cytokine treatment in panel B) using a one-way ANOVA, followed by Dunnett's multiple comparison test: ***, P
Figure Legend Snippet: Investigation of CSFV-infected pDC-derived cytokines on the MHC class II expression of NK and γδ T cells. (A) pDCs or mDCs were exposed to CSFV C-strain, Brescia strain, or a mock virus-infected cryolysate supernatant, and after 24 h in culture, the levels of TNF-α and type I IFN in the supernatants were analyzed using a porcine cytokine multiplex ELISA and Mx/CAT reporter gene assay, respectively. (B) Enriched NK and γδ T cells were then cultured in cRPMI medium alone or in the presence of porcine recombinant IFN-α and/or TNF-α, at concentrations comparable to those detected in infected pDC cocultures, and the expression of MHC-II was assessed by flow cytometry after 24 h. The mean ± SEM data from three independent experiments utilizing different animals are shown. The values for each group were compared to the corresponding unstimulated control (mock stimulation in panel A and no cytokine treatment in panel B) using a one-way ANOVA, followed by Dunnett's multiple comparison test: ***, P

Techniques Used: Infection, Derivative Assay, Expressing, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Reporter Gene Assay, Cell Culture, Recombinant, Flow Cytometry, Cytometry

Quantification of local and systemic type I IFN following C-strain vaccination and/or virulent CSFV Brescia challenge. Serum and tonsils were collected longitudinally following C-strain vaccination and challenge 5 days later with CSFV Brescia strain. Cells expressing IFN-α were enumerated in the interfollicular areas (closed symbols) and follicles (open symbols) of the tonsils by immunohistochemistry (A), and the levels of type I IFN in serum were determined using an Mx/CAT reporter gene assay (B). The data represent the mean of 3 pigs/group ± SEM. Statistical analyses were performed using a one-way ANOVA, followed by Bonferroni's multiple comparison test: ***, P
Figure Legend Snippet: Quantification of local and systemic type I IFN following C-strain vaccination and/or virulent CSFV Brescia challenge. Serum and tonsils were collected longitudinally following C-strain vaccination and challenge 5 days later with CSFV Brescia strain. Cells expressing IFN-α were enumerated in the interfollicular areas (closed symbols) and follicles (open symbols) of the tonsils by immunohistochemistry (A), and the levels of type I IFN in serum were determined using an Mx/CAT reporter gene assay (B). The data represent the mean of 3 pigs/group ± SEM. Statistical analyses were performed using a one-way ANOVA, followed by Bonferroni's multiple comparison test: ***, P

Techniques Used: Expressing, Immunohistochemistry, Reporter Gene Assay

5) Product Images from "In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses"

Article Title: In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses

Journal: BMC Veterinary Research

doi: 10.1186/s12917-018-1675-x

Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p
Figure Legend Snippet: Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

6) Product Images from "In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses"

Article Title: In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses

Journal: BMC Veterinary Research

doi: 10.1186/s12917-018-1675-x

Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p
Figure Legend Snippet: Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

mRNA transcription of cytokines in PBMCs collected from pigs infected with the VR2332c and JA142c PRRSV strains at 0, 7, 14, 21 and 28 dpc. Analysis of mRNA transcription in PBMCs (n = 5) collected from infected and control pigs using real-time PCR. Asterisks indicate significant differences in cytokine expression induced by each virus compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05)
Figure Legend Snippet: mRNA transcription of cytokines in PBMCs collected from pigs infected with the VR2332c and JA142c PRRSV strains at 0, 7, 14, 21 and 28 dpc. Analysis of mRNA transcription in PBMCs (n = 5) collected from infected and control pigs using real-time PCR. Asterisks indicate significant differences in cytokine expression induced by each virus compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05)

Techniques Used: Infection, Real-time Polymerase Chain Reaction, Expressing

Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

7) Product Images from "In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses"

Article Title: In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses

Journal: BMC Veterinary Research

doi: 10.1186/s12917-018-1675-x

Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p
Figure Legend Snippet: Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

8) Product Images from "Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine"

Article Title: Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine

Journal: Porcine Health Management

doi: 10.1186/s40813-018-0112-7

Evolution of IFN-α levels (Limit of detection: 3.9 pg/mL)
Figure Legend Snippet: Evolution of IFN-α levels (Limit of detection: 3.9 pg/mL)

Techniques Used:

9) Product Images from "In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses"

Article Title: In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses

Journal: BMC Veterinary Research

doi: 10.1186/s12917-018-1675-x

Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p
Figure Legend Snippet: Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

10) Product Images from "In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses"

Article Title: In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses

Journal: BMC Veterinary Research

doi: 10.1186/s12917-018-1675-x

Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p
Figure Legend Snippet: Serum cytokine levels in PRRSV-infected pigs. Sera collected from pigs (n = 5) at 0, 3, 7, 14, 21 and 28 dpc were analysed for a IFN-α; b TNF-α; c IL-12; d IL-10; and e IL-4 by ELISA. Each bar represents the average cytokine amount from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PAM cultures. a mRNA and b protein expression of cytokines in PAMs and supernatants ( n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 24 and 36 hpi. Asterisks indicate significant differences in the cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)
Figure Legend Snippet: Cytokine expression in PBMC cultures. a mRNA and b protein expression of cytokines in PBMCs and supernatants (n = 6), as determined by real-time PCR and ELISA, respectively, at 12, 36 and 72 hpi. Asterisks indicate significant differences in cytokine expression induced by each virus or stimulant compared with mock treatment or significant differences between JA142c and VR2332c (* indicates p ≤ 0.05, ** indicates p ≤ 0.01)

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

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Article Title: Innate immune responses to human rotavirus in the neonatal gnotobiotic piglet disease model
Article Snippet: .. Then cells were washed and stained with mAbs to IFN-α (IgG1 isotype, clone 27105-1; R & D Systems, Minneapolis, MN). .. After incubation, cells were washed twice and allophycocyanin-conjugated anti-mouse IgG1 (Becton Dickinson) was added.

Incubation:

Article Title: Partial Activation of Natural Killer and γδ T Cells by Classical Swine Fever Viruses Is Associated with Type I Interferon Elicited from Plasmacytoid Dendritic Cells
Article Snippet: .. A mouse MAb against porcine IFN-α (clone F17; R & D Systems) was added at 1.82 μg/ml and sections incubated at 4°C for 18 to 20 h. The sections were washed in TBST and incubated for 30 min with a 1:100 dilution of biotinylated secondary antibody (biotin-SP-conjugated AffiniPure Fab fragment goat anti-mouse IgG [H+L]; Jackson ImmunoResearch, Stratech, Newmarket, United Kingdom), and normal goat serum (1:66 dilution) and normal pig serum (1:33 dilution) were added before being washed twice in TBST. .. The sections were incubated for 30 min at room temperature with avidin-biotin complex (ABC Vector Elite; Vector Laboratories, Peterborough, United Kingdom), and the signal was detected using 3,3′-diaminobenzidine tetrahydrochloride (DAB).

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    R&D Systems biotinylated pig ifn α
    Evolution of <t>IFN-α</t> levels (Limit of detection: 3.9 pg/mL)
    Biotinylated Pig Ifn α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated pig ifn α/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated pig ifn α - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    R&D Systems detection antibody
    Evolution of <t>IFN-α</t> levels (Limit of detection: 3.9 pg/mL)
    Detection Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/detection antibody/product/R&D Systems
    Average 92 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    detection antibody - by Bioz Stars, 2020-09
    92/100 stars
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    Evolution of IFN-α levels (Limit of detection: 3.9 pg/mL)

    Journal: Porcine Health Management

    Article Title: Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine

    doi: 10.1186/s40813-018-0112-7

    Figure Lengend Snippet: Evolution of IFN-α levels (Limit of detection: 3.9 pg/mL)

    Article Snippet: Briefly, plates were coated with anti-pig IFN-α (mAb K9, R & D systems) at 1.1 Ug/mL; 50 μl of each sample and biotinylated pig IFN-α (mAb F17, R & D systems) were added.

    Techniques: