Goat Anti Rabbit IgG Fab Mouse Human SP ads UNLB
Pooled antisera from goats hyperimmunized with rabbit IgG
Quality tested applications for relevant formats include -ELISA
https://www.bioz.com/result/biotinylated polyclonal goat f ab 2 anti igg2a/product/SouthernBiotech
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1) Product Images from "Cross-reactive antigen expressed by B6 splenocytes drives receptor editing and MZ differentiation of IgG2a-reactive AM14 Vκ8 B cells"
Article Title: Cross-reactive antigen expressed by B6 splenocytes drives receptor editing and MZ differentiation of IgG2a-reactive AM14 Vκ8 B cells
Journal: Journal of immunology (Baltimore, Md. : 1950)
Figure Legend Snippet: B6 AM14 Vκ8 B cells have an anergic phenotype A. Serum IgG2a levels of AM14 Vκ8/Vκ8 BALB/c (○) and B6 (π) mice were measured by ELISA (n=5 BALB/c, n=11 B6, mean +/− SEM). B. Number of B220+ B cells in the spleen of AM14 Vκ8/Vκ8 BALB/c (white bar, n=19) and B6 (black bar, n=9). Mean +/− SEM is shown. C. Proliferation of B220-purified splenic B cells in response to 15 μg/ml goat anti-IgM F(ab’) 2 (left) or 1 μg/ml ODN1826 (right) was measured by 3 H-thymidine uptake after 30h (n=7 BALB/c and B6, mean +/− SEM). D. Distribution of CD23 hi CD21 lo FO and CD23 lo CD21 hi MZ B cells of B220 + AA4.1 − mature B cells in the spleen was ascertained by flow cytometry (representative flow plots are shown, summary plot of n=19 BALB/c, n=9 B6 is shown on the right, mean +/− SEM). E. CD23-purified splenic B cells from AM14 Vκ8/Vκ8 BALB/c (○) and B6 (π) mice were stimulated with anti-IgM (left) and ODN1826 (right) and proliferation was measured as in C (n=9 BALB/c, n=7 B6, mean +/− SEM). F. Surface expression levels of the AM14 Vκ8 BCR (4G7), IgM and IgD on AM14 Vκ8/Vκ8 BALB/c B cells (white bar) and AM14 Vκ8/Vκ8 B6 (black bar) were measured by flow cytometry. Mean fluorescent intensity (MFI) was calculated using FlowJo software. The mean +/− SEM of n=6 mice is shown. G. B cell subsets in the bone marrow of AM14 Vκ8/Vκ8 BALB/c and B6 mice were analyzed using flow cytometry according to Hardy fractions B-D (B220 low IgM neg ), E (B220 low IgM + ) and F (B220 hi IgM + ). A summary graph of the different subsets is shown on the right for AM14 Vκ8/Vκ8 BALB/c mice (white bar, n=16) and AM14 Vκ8/Vκ8 B6 mice (black bar, n=14). Mean +/− SEM is shown.
Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification, Flow Cytometry, Expressing, Software