Structured Review

SouthernBiotech biotinylated goat f
Biotinylated Goat F, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated goat f/product/SouthernBiotech
Average 89 stars, based on 30 article reviews
Price from $9.99 to $1999.99
biotinylated goat f - by Bioz Stars, 2020-07
89/100 stars

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Staining:

Article Title: CD40L+ CD4+ memory T cells migrate in a CD62P-dependent fashion into reactive lymph nodes and license dendritic cells for T cell priming
Article Snippet: .. CD62P ligands on T cells were detected by staining with a human IgG-CD62P fusion protein (BD Biosciences), followed by incubation with a biotinylated-goat F(ab′)2 anti–human IgG antibody (SouthernBiotech) and streptavidin-allophycocyanin (APC; Invitrogen). .. Cytokine production was measured by intracellular staining after 4 h of stimulation with 10−7 M PMA and 1 μg/ml ionomycin (both from Sigma-Aldrich), with the last 2 h of culture in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich).

Incubation:

Article Title: Improved serum-free culture conditions for spleen-derived murine fibrocytes
Article Snippet: .. Slides were then washed in 5 changes of 50 ml PBS over 15 minutes and incubated for 30 minutes in PBS/BSA with 2.5 μg/ml biotinylated mouse F(ab′)2 anti-rat IgG (Jackson Immunoresearch) or 2.5 μg/ml biotinylated goat F(ab′)2 anti-rabbit IgG (Southern Biotech, Birmingham, AL). .. For fibrocyte collagen analysis, spleen cells were cultured for 5 days in the presence of IL-13 and M-CSF.

Article Title: The Differentiation in vitro of Human Tonsil B Cells With the Phenotypic and Functional Characteristics of T-bet+ Atypical Memory B Cells in Malaria
Article Snippet: .. To bind Ag to the PLB, the wells containing PLB were incubated at RT with 2.5 μg/ml streptavidin for 10 min, followed by 1 μg/ml biotinylated goat F(ab′)2 anti-human λ + κ (Southern biotech) for 20 min. PMS was prepared in 8-well Lab-Tek chamber (#1.5 Borosilicate coverglass system, Nunc) as previously described ( ). .. Briefly, 293A cells (1 × 105 ) were seeded in poly-l-lysine-coated wells and cultured overnight in complete RPMI at 37°C, 80–90% confluency being achieved.

Article Title: Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation
Article Snippet: .. Slides were then washed in six changes of PBS over 30 min and incubated for 30 min with 2.5 μg/ml biotinylated goat F(ab′)2 anti-mouse IgG or biotinylated goat F(ab′)2 anti-rabbit IgG (both cross-adsorbed against human Ig, Southern Biotechnology). .. After washing, the biotinylated antibodies were detected by ExtrAvidin alkaline phosphatase (Sigma-Aldrich) in PBS containing 4% BSA.

Article Title: Aggregated IgG inhibits the differentiation of human fibrocytes
Article Snippet: .. Slides were then washed in three changes of PBS over 15 min and incubated for 30 min with 2.5 µg/ml biotinylated goat F(ab′)2 anti-mouse IgG or biotinylated goat F(ab′)2 anti-rabbit IgG (cross-adsorbed against human Ig, Southern Biotechnology Associates Inc.). .. After washing, the biotinylated antibodies were detected by ExtrAvidin peroxidase (Sigma-Aldrich).

Article Title: CD40L+ CD4+ memory T cells migrate in a CD62P-dependent fashion into reactive lymph nodes and license dendritic cells for T cell priming
Article Snippet: .. CD62P ligands on T cells were detected by staining with a human IgG-CD62P fusion protein (BD Biosciences), followed by incubation with a biotinylated-goat F(ab′)2 anti–human IgG antibody (SouthernBiotech) and streptavidin-allophycocyanin (APC; Invitrogen). .. Cytokine production was measured by intracellular staining after 4 h of stimulation with 10−7 M PMA and 1 μg/ml ionomycin (both from Sigma-Aldrich), with the last 2 h of culture in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich).

Article Title: Inhibition of Fibrocyte Differentiation by Serum Amyloid P
Article Snippet: .. Slides were then washed in three changes of PBS over 15 min and incubated for 60 min with biotinylated goat F(ab′)2 anti-mouse Ig (Southern Biotechnology, Birmingham, AL). .. After washing, the biotinylated Abs were detected by ExtrAvidin peroxidase (Sigma-Aldrich).

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  • 89
    SouthernBiotech biotinylated goat f
    Biotinylated Goat F, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat f/product/SouthernBiotech
    Average 89 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat f - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    93
    SouthernBiotech biotinylated 0110 08 goat f
    Biotinylated 0110 08 Goat F, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated 0110 08 goat f/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated 0110 08 goat f - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    94
    SouthernBiotech biotinylated goat anti human igm f
    Biotinylated Goat Anti Human Igm F, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti human igm f/product/SouthernBiotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti human igm f - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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