biotinylated goat anti human igm f  (SouthernBiotech)

 
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  • 94
    Name:
    Goat F ab 2 Anti Human IgM BIOT
    Description:

    Catalog Number:
    2022-08
    Price:
    None
    Applications:
    Quality tested applications for relevant formats include -ELISA 1FLISAFlow Cytometry 3,4 Other referenced applications for relevant formats include -Immunocytochemistry 15ELISpot 2Stimulation 5-14
    Isotype:
    Goat F(ab')2 IgG
    Source:
    Pepsin digest of Goat Anti-Human IgM (SB Cat. No. 2020)
    Format:
    BIOT (Biotin)
    Buy from Supplier


    Structured Review

    SouthernBiotech biotinylated goat anti human igm f
    Goat F ab 2 Anti Human IgM BIOT

    https://www.bioz.com/result/biotinylated goat anti human igm f/product/SouthernBiotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti human igm f - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Transduction:

    Article Title: A PATHOBIOLOGICAL ROLE OF THE INSULIN RECEPTOR IN CHRONIC LYMPHOCYTIC LEUKEMIA
    Article Snippet: .. For analysis of insulin-induced signal transduction, negatively selected CD19+ cells from CLL cases with and without INSR expression were incubated for 1.5 hours in RPMI medium without serum and subsequently stimulated with 10nM insulin or biotinylated goat anti-human IgM F(ab)2 fragments (Southern Biotech #2022-08) together with avidin for 20 minutes. ..

    Flow Cytometry:

    Article Title: HDAC Inhibitors Upregulate B Cell microRNAs that Silence AID and Blimp-1 Expression for Epigenetic Modulation of Antibody and Autoantibody Responses
    Article Snippet: .. Naïve B cells were then cultured in FBS-RPMI and stimulate with CD154 (10 U/ml), IL-4 (20 ng/ml; R & D Systems) and IL-21 (50 ng/ml; R & D Systems) or CD154 (10 U/ml), IL-21 (50 ng/ml) and TGF-β (0.5 ng/ml) in the presence of nil, VPA or butyrate for 120 h. B cells were then stained with 7-AAD, FITC-anti-IgM mAb (F5384; Sigma-Aldrich), PE-anti-CD19 mAb (HIB19; BioLegend) and allophycocyanin (APC)-anti-IgG mAb (G18-145; BD Biosciences) or 7-AAD, FITC-anti-IgA mAb (F5259; Sigma-Aldrich), PE-anti-CD19 mAb (HIB19; BioLegend) and biotin-F(ab’)2 anti-IgM (2022-08; Southern Biotech), followed by APC-streptavidin and analyzed by flow cytometry. .. For plasma cell differentiation, cells were stained with 7-aminoactinomycin D (7-AAD; BD Biosciences), APC-anti-CD27 (LG.3A10; BioLegend) and FITC-anti-CD38 (31014X; BD Biosciences) and analyzed by flow cytometry.

    Article Title: B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids
    Article Snippet: .. Naive B cells were then cultured in FCS-RPMI and stimulated with CD154 (10 U ml−1 ), IL-4 (20 ng ml−1 , R & D Systems), and IL-21 (50 ng ml−1 , R & D Systems) or CD154 (10 U ml−1 ), IL-21 (50 ng ml−1 ), and TGF-β (0.5 ng ml−1 ) in the presence of nil, butyrate, propionate, or butyrate plus propionate for 120 h. B cells were then stained with 7-aminoactinomycin D (7-AAD), FITC–anti-IgM mAb (314506, BioLegend), PE–anti-CD19 mAb (302208, BioLegend), and allophycocyanin-anti-IgG mAb (562025, BD Biosciences) or 7-AAD, FITC–anti-IgA mAb (F5259, Sigma-Aldrich), and biotin-F(ab′)2 anti-IgM (2022-08, Southern Biotech), followed by allophycocyanin–streptavidin, and analyzed by flow cytometry. .. Immunoblotting B cells from C57BL/6 mice were stimulated with LPS (3 μg ml−1 ) plus IL-4 (4 ng ml−1 ) in the presence of nil, butyrate (500 μM), propionate (2000 μM), or butyrate (500 μM) plus propionate (2000 μM) for 72 h. Cells were harvested and lysed in Laemmli buffer.

    Cytometry:

    Article Title: HDAC Inhibitors Upregulate B Cell microRNAs that Silence AID and Blimp-1 Expression for Epigenetic Modulation of Antibody and Autoantibody Responses
    Article Snippet: .. Naïve B cells were then cultured in FBS-RPMI and stimulate with CD154 (10 U/ml), IL-4 (20 ng/ml; R & D Systems) and IL-21 (50 ng/ml; R & D Systems) or CD154 (10 U/ml), IL-21 (50 ng/ml) and TGF-β (0.5 ng/ml) in the presence of nil, VPA or butyrate for 120 h. B cells were then stained with 7-AAD, FITC-anti-IgM mAb (F5384; Sigma-Aldrich), PE-anti-CD19 mAb (HIB19; BioLegend) and allophycocyanin (APC)-anti-IgG mAb (G18-145; BD Biosciences) or 7-AAD, FITC-anti-IgA mAb (F5259; Sigma-Aldrich), PE-anti-CD19 mAb (HIB19; BioLegend) and biotin-F(ab’)2 anti-IgM (2022-08; Southern Biotech), followed by APC-streptavidin and analyzed by flow cytometry. .. For plasma cell differentiation, cells were stained with 7-aminoactinomycin D (7-AAD; BD Biosciences), APC-anti-CD27 (LG.3A10; BioLegend) and FITC-anti-CD38 (31014X; BD Biosciences) and analyzed by flow cytometry.

    Article Title: B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids
    Article Snippet: .. Naive B cells were then cultured in FCS-RPMI and stimulated with CD154 (10 U ml−1 ), IL-4 (20 ng ml−1 , R & D Systems), and IL-21 (50 ng ml−1 , R & D Systems) or CD154 (10 U ml−1 ), IL-21 (50 ng ml−1 ), and TGF-β (0.5 ng ml−1 ) in the presence of nil, butyrate, propionate, or butyrate plus propionate for 120 h. B cells were then stained with 7-aminoactinomycin D (7-AAD), FITC–anti-IgM mAb (314506, BioLegend), PE–anti-CD19 mAb (302208, BioLegend), and allophycocyanin-anti-IgG mAb (562025, BD Biosciences) or 7-AAD, FITC–anti-IgA mAb (F5259, Sigma-Aldrich), and biotin-F(ab′)2 anti-IgM (2022-08, Southern Biotech), followed by allophycocyanin–streptavidin, and analyzed by flow cytometry. .. Immunoblotting B cells from C57BL/6 mice were stimulated with LPS (3 μg ml−1 ) plus IL-4 (4 ng ml−1 ) in the presence of nil, butyrate (500 μM), propionate (2000 μM), or butyrate (500 μM) plus propionate (2000 μM) for 72 h. Cells were harvested and lysed in Laemmli buffer.

    Cell Culture:

    Article Title: HDAC Inhibitors Upregulate B Cell microRNAs that Silence AID and Blimp-1 Expression for Epigenetic Modulation of Antibody and Autoantibody Responses
    Article Snippet: .. Naïve B cells were then cultured in FBS-RPMI and stimulate with CD154 (10 U/ml), IL-4 (20 ng/ml; R & D Systems) and IL-21 (50 ng/ml; R & D Systems) or CD154 (10 U/ml), IL-21 (50 ng/ml) and TGF-β (0.5 ng/ml) in the presence of nil, VPA or butyrate for 120 h. B cells were then stained with 7-AAD, FITC-anti-IgM mAb (F5384; Sigma-Aldrich), PE-anti-CD19 mAb (HIB19; BioLegend) and allophycocyanin (APC)-anti-IgG mAb (G18-145; BD Biosciences) or 7-AAD, FITC-anti-IgA mAb (F5259; Sigma-Aldrich), PE-anti-CD19 mAb (HIB19; BioLegend) and biotin-F(ab’)2 anti-IgM (2022-08; Southern Biotech), followed by APC-streptavidin and analyzed by flow cytometry. .. For plasma cell differentiation, cells were stained with 7-aminoactinomycin D (7-AAD; BD Biosciences), APC-anti-CD27 (LG.3A10; BioLegend) and FITC-anti-CD38 (31014X; BD Biosciences) and analyzed by flow cytometry.

    Article Title: B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids
    Article Snippet: .. Naive B cells were then cultured in FCS-RPMI and stimulated with CD154 (10 U ml−1 ), IL-4 (20 ng ml−1 , R & D Systems), and IL-21 (50 ng ml−1 , R & D Systems) or CD154 (10 U ml−1 ), IL-21 (50 ng ml−1 ), and TGF-β (0.5 ng ml−1 ) in the presence of nil, butyrate, propionate, or butyrate plus propionate for 120 h. B cells were then stained with 7-aminoactinomycin D (7-AAD), FITC–anti-IgM mAb (314506, BioLegend), PE–anti-CD19 mAb (302208, BioLegend), and allophycocyanin-anti-IgG mAb (562025, BD Biosciences) or 7-AAD, FITC–anti-IgA mAb (F5259, Sigma-Aldrich), and biotin-F(ab′)2 anti-IgM (2022-08, Southern Biotech), followed by allophycocyanin–streptavidin, and analyzed by flow cytometry. .. Immunoblotting B cells from C57BL/6 mice were stimulated with LPS (3 μg ml−1 ) plus IL-4 (4 ng ml−1 ) in the presence of nil, butyrate (500 μM), propionate (2000 μM), or butyrate (500 μM) plus propionate (2000 μM) for 72 h. Cells were harvested and lysed in Laemmli buffer.

    Avidin-Biotin Assay:

    Article Title: A PATHOBIOLOGICAL ROLE OF THE INSULIN RECEPTOR IN CHRONIC LYMPHOCYTIC LEUKEMIA
    Article Snippet: .. For analysis of insulin-induced signal transduction, negatively selected CD19+ cells from CLL cases with and without INSR expression were incubated for 1.5 hours in RPMI medium without serum and subsequently stimulated with 10nM insulin or biotinylated goat anti-human IgM F(ab)2 fragments (Southern Biotech #2022-08) together with avidin for 20 minutes. ..

    Article Title: Temporal genetic program following B-cell receptor cross-linking: altered balance between proliferation and death in healthy and malignant B cells
    Article Snippet: .. One was used as unstimulated (US) control, and the other was stimulated (S) by goat F(ab')2 anti–human IgM-BIOT (Southern Biotechnology, Birmingham, AL) at 20 μg/mL and cross-linked by 20 μg/mL avidin (Sigma-Aldrich, St Louis, MO) on ice for 5 minutes, followed by incubation at 37°C for 15 minutes. .. The amount and duration of stimulation were determined in a pilot study evaluating the apoptosis response assessed by flow cytometry as described below under “Apoptosis assay” as well as protein tyrosine kinase phosphorylation immunoblotting (data not shown).

    Concentration Assay:

    Article Title: Rewiring of sIgM-Mediated Intracellular Signaling through the CD180 Toll-like Receptor
    Article Snippet: .. PBMCs or purified CD19+ cells were incubated with anti-CD180 mAb for 10 to 20 min or with goat anti-human IgM F(ab)2 (Southern Biotech, Birmingham, AL, USA) for 10 min at a final concentration 20 μg/mL at 37°C and 5% CO2 . ..

    Incubation:

    Article Title: A PATHOBIOLOGICAL ROLE OF THE INSULIN RECEPTOR IN CHRONIC LYMPHOCYTIC LEUKEMIA
    Article Snippet: .. For analysis of insulin-induced signal transduction, negatively selected CD19+ cells from CLL cases with and without INSR expression were incubated for 1.5 hours in RPMI medium without serum and subsequently stimulated with 10nM insulin or biotinylated goat anti-human IgM F(ab)2 fragments (Southern Biotech #2022-08) together with avidin for 20 minutes. ..

    Article Title: Temporal genetic program following B-cell receptor cross-linking: altered balance between proliferation and death in healthy and malignant B cells
    Article Snippet: .. One was used as unstimulated (US) control, and the other was stimulated (S) by goat F(ab')2 anti–human IgM-BIOT (Southern Biotechnology, Birmingham, AL) at 20 μg/mL and cross-linked by 20 μg/mL avidin (Sigma-Aldrich, St Louis, MO) on ice for 5 minutes, followed by incubation at 37°C for 15 minutes. .. The amount and duration of stimulation were determined in a pilot study evaluating the apoptosis response assessed by flow cytometry as described below under “Apoptosis assay” as well as protein tyrosine kinase phosphorylation immunoblotting (data not shown).

    Article Title: Rewiring of sIgM-Mediated Intracellular Signaling through the CD180 Toll-like Receptor
    Article Snippet: .. PBMCs or purified CD19+ cells were incubated with anti-CD180 mAb for 10 to 20 min or with goat anti-human IgM F(ab)2 (Southern Biotech, Birmingham, AL, USA) for 10 min at a final concentration 20 μg/mL at 37°C and 5% CO2 . ..

    Article Title: Internalization of B Cell Receptors in Human EU12 μHC+ Immature B Cells Specifically Alters Downstream Signaling Events
    Article Snippet: .. Approximately 1 × 106 cells were incubated with goat anti-human IgM antibody F(ab′)2 fragments (2 μ g/mL) for different human cell lines or goat anti-mouse IgM antibody F(ab′)2 fragments (5 μ g/mL) (Southern Biotech, AL) at 37°C. .. Cells were collected at different time points and cell surface BCR levels were analyzed by flow cytometry.

    Expressing:

    Article Title: A PATHOBIOLOGICAL ROLE OF THE INSULIN RECEPTOR IN CHRONIC LYMPHOCYTIC LEUKEMIA
    Article Snippet: .. For analysis of insulin-induced signal transduction, negatively selected CD19+ cells from CLL cases with and without INSR expression were incubated for 1.5 hours in RPMI medium without serum and subsequently stimulated with 10nM insulin or biotinylated goat anti-human IgM F(ab)2 fragments (Southern Biotech #2022-08) together with avidin for 20 minutes. ..

    Staining:

    Article Title: HDAC Inhibitors Upregulate B Cell microRNAs that Silence AID and Blimp-1 Expression for Epigenetic Modulation of Antibody and Autoantibody Responses
    Article Snippet: .. Naïve B cells were then cultured in FBS-RPMI and stimulate with CD154 (10 U/ml), IL-4 (20 ng/ml; R & D Systems) and IL-21 (50 ng/ml; R & D Systems) or CD154 (10 U/ml), IL-21 (50 ng/ml) and TGF-β (0.5 ng/ml) in the presence of nil, VPA or butyrate for 120 h. B cells were then stained with 7-AAD, FITC-anti-IgM mAb (F5384; Sigma-Aldrich), PE-anti-CD19 mAb (HIB19; BioLegend) and allophycocyanin (APC)-anti-IgG mAb (G18-145; BD Biosciences) or 7-AAD, FITC-anti-IgA mAb (F5259; Sigma-Aldrich), PE-anti-CD19 mAb (HIB19; BioLegend) and biotin-F(ab’)2 anti-IgM (2022-08; Southern Biotech), followed by APC-streptavidin and analyzed by flow cytometry. .. For plasma cell differentiation, cells were stained with 7-aminoactinomycin D (7-AAD; BD Biosciences), APC-anti-CD27 (LG.3A10; BioLegend) and FITC-anti-CD38 (31014X; BD Biosciences) and analyzed by flow cytometry.

    Article Title: B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids
    Article Snippet: .. Naive B cells were then cultured in FCS-RPMI and stimulated with CD154 (10 U ml−1 ), IL-4 (20 ng ml−1 , R & D Systems), and IL-21 (50 ng ml−1 , R & D Systems) or CD154 (10 U ml−1 ), IL-21 (50 ng ml−1 ), and TGF-β (0.5 ng ml−1 ) in the presence of nil, butyrate, propionate, or butyrate plus propionate for 120 h. B cells were then stained with 7-aminoactinomycin D (7-AAD), FITC–anti-IgM mAb (314506, BioLegend), PE–anti-CD19 mAb (302208, BioLegend), and allophycocyanin-anti-IgG mAb (562025, BD Biosciences) or 7-AAD, FITC–anti-IgA mAb (F5259, Sigma-Aldrich), and biotin-F(ab′)2 anti-IgM (2022-08, Southern Biotech), followed by allophycocyanin–streptavidin, and analyzed by flow cytometry. .. Immunoblotting B cells from C57BL/6 mice were stimulated with LPS (3 μg ml−1 ) plus IL-4 (4 ng ml−1 ) in the presence of nil, butyrate (500 μM), propionate (2000 μM), or butyrate (500 μM) plus propionate (2000 μM) for 72 h. Cells were harvested and lysed in Laemmli buffer.

    Purification:

    Article Title: Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells
    Article Snippet: .. Primary human B cells purified from blood of healthy donors were prepared as described above and stimulated with 15 µg/ml goat anti-human IgM F(ab’)2 (SouthernBiotech). .. The PLC inhibitor U73122 (Tocris Bioscience) was added to the cells at the indicated concentrations 1 minute before BCR stimulation.

    Article Title: Rewiring of sIgM-Mediated Intracellular Signaling through the CD180 Toll-like Receptor
    Article Snippet: .. PBMCs or purified CD19+ cells were incubated with anti-CD180 mAb for 10 to 20 min or with goat anti-human IgM F(ab)2 (Southern Biotech, Birmingham, AL, USA) for 10 min at a final concentration 20 μg/mL at 37°C and 5% CO2 . ..

    Immunofluorescence:

    Article Title: Internalization of B Cell Receptors in Human EU12 μHC+ Immature B Cells Specifically Alters Downstream Signaling Events
    Article Snippet: .. Antibodies and Reagents Immunofluorescence assays were performed using the following antibodies: Cy5-labeled AffiniPure donkey anti-human IgM F(ab′)2 fragment (Jackson ImmunoResearch, West Grove, PA), goat anti-human IgM (μ chain specific, LE/AF) F(ab′)2 fragments (Southern Biotech, Birmingham, AL), goat anti-rabbit Alexa-488 (Invitrogen, Carlsbad, CA), anti-phospho-SYK, phospho-ERK, and phospho-CD19 antibodies (Cell Signaling Technology, Danvers, MA). .. Cell Surface Staining and Flow Cytometric Analysis For cell surface staining, cells (1 × 106 cells) were blocked with PBS buffer with 2% FBS and stained with Cy5-labeled anti-human IgM F(ab′)2 antibodies, FITC-labeled anti-HLA antibodies, or APC-labeled anti-CD86 antibodies on ice for 30 min [ ].

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  • Bioz Stars
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  • 94
    SouthernBiotech biotinylated goat anti human igm f
    Biotinylated Goat Anti Human Igm F, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti human igm f/product/SouthernBiotech
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti human igm f - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    SouthernBiotech mono biotinylated goat anti human igm f
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    https://www.bioz.com/result/mono biotinylated goat anti human igm f/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
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    mono biotinylated goat anti human igm f - by Bioz Stars, 2020-08
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      Buy from Supplier

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    SouthernBiotech biotinylated goat f
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    https://www.bioz.com/result/biotinylated goat f/product/SouthernBiotech
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    Price from $9.99 to $1999.99
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