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R&D Systems biotinylated ephrin fc proteins
Binding interactions of <t>ephrin</t> Fc fusion proteins with Eph receptors. Apparent dissociation constant (K D ) values were obtained from curves measuring the binding of <t>biotinylated</t> ephrin Fc proteins to Eph receptor Fc proteins immobilized on ELISA plates.
Biotinylated Ephrin Fc Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated ephrin fc proteins/product/R&D Systems
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotinylated ephrin fc proteins - by Bioz Stars, 2020-07
85/100 stars

Related Products / Commonly Used Together

streptavidin
tbst
horseradish peroxidase hrp

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1) Product Images from "Profiling Eph receptor expression in cells and tissues"

Article Title: Profiling Eph receptor expression in cells and tissues

Journal: Cell Adhesion & Migration

doi: 10.4161/cam.19620

Binding interactions of ephrin Fc fusion proteins with Eph receptors. Apparent dissociation constant (K D ) values were obtained from curves measuring the binding of biotinylated ephrin Fc proteins to Eph receptor Fc proteins immobilized on ELISA plates.
Figure Legend Snippet: Binding interactions of ephrin Fc fusion proteins with Eph receptors. Apparent dissociation constant (K D ) values were obtained from curves measuring the binding of biotinylated ephrin Fc proteins to Eph receptor Fc proteins immobilized on ELISA plates.

Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay

Related Articles

Incubation:

Article Title: Profiling Eph receptor expression in cells and tissues
Article Snippet: .. After washing, the wells were incubated for 2 h with different concentrations of biotinylated ephrin Fc proteins (R & D Systems) in TBST (150 mM NaCl, 50 mM TRIS-HCl, pH 7.5 with 0.01% Tween 20) at room temperature, washed, and then incubated for 30 min with 0.5 μg/ml streptavidin conjugated to horseradish peroxidase (HRP) (Thermo Fisher Scientific, #21127) in TBST. .. Bound HRP was quantified by adding 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS, Sigma-Aldrich, #A1888) in citric acid as a substrate and reading the absorbance at 405 nm.

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  • 91
    R&D Systems biotinylated ephrin a1 fc
    Compound 2 competitively inhibits <t>EphA2-ephrin-A1</t> binding. A) compound 2 dose-dependently displaced binding of <t>ephrin-A1-Fc</t> from immobilized EphA2-Fc. B) binding of ephrin-A1-Fc to immobilized EphA2-Fc in the presence of different concentrations of compound
    Biotinylated Ephrin A1 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated ephrin a1 fc/product/R&D Systems
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    biotinylated ephrin a1 fc - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    85
    R&D Systems biotinylated ephrin fc proteins
    Binding interactions of <t>ephrin</t> Fc fusion proteins with Eph receptors. Apparent dissociation constant (K D ) values were obtained from curves measuring the binding of <t>biotinylated</t> ephrin Fc proteins to Eph receptor Fc proteins immobilized on ELISA plates.
    Biotinylated Ephrin Fc Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated ephrin fc proteins/product/R&D Systems
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated ephrin fc proteins - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    Compound 2 competitively inhibits EphA2-ephrin-A1 binding. A) compound 2 dose-dependently displaced binding of ephrin-A1-Fc from immobilized EphA2-Fc. B) binding of ephrin-A1-Fc to immobilized EphA2-Fc in the presence of different concentrations of compound

    Journal: Journal of medicinal chemistry

    Article Title: Amino acid conjugates of lithocholic acid as antagonists of the EphA2 receptor

    doi: 10.1021/jm301890k

    Figure Lengend Snippet: Compound 2 competitively inhibits EphA2-ephrin-A1 binding. A) compound 2 dose-dependently displaced binding of ephrin-A1-Fc from immobilized EphA2-Fc. B) binding of ephrin-A1-Fc to immobilized EphA2-Fc in the presence of different concentrations of compound

    Article Snippet: During the experiment to determine selectivity of compounds, all EphAs (R & D Systems SMPK1) and EphBs (R & D Systems SMPK2) receptors were incubated overnight similarly to EphA2 as previously described; 150 ng/ml biotinylated ephrin-A1-Fc or biotinylated ephrin-B1-Fc (R & D Systems BT473) was used with EphAs or EphBs, respectively.

    Techniques: Binding Assay

    Compound 20 dose-dependently displaces the binding of ephrin-A1-Fc and the ephrin-B1-Fc ectodomains from immobilized EphA-Fc or EphB-Fc ectodomains, respectively. Tested concentrations: 30 μM (black bar), 10 μM (checkered bar), 3 μM

    Journal: Journal of medicinal chemistry

    Article Title: Amino acid conjugates of lithocholic acid as antagonists of the EphA2 receptor

    doi: 10.1021/jm301890k

    Figure Lengend Snippet: Compound 20 dose-dependently displaces the binding of ephrin-A1-Fc and the ephrin-B1-Fc ectodomains from immobilized EphA-Fc or EphB-Fc ectodomains, respectively. Tested concentrations: 30 μM (black bar), 10 μM (checkered bar), 3 μM

    Article Snippet: During the experiment to determine selectivity of compounds, all EphAs (R & D Systems SMPK1) and EphBs (R & D Systems SMPK2) receptors were incubated overnight similarly to EphA2 as previously described; 150 ng/ml biotinylated ephrin-A1-Fc or biotinylated ephrin-B1-Fc (R & D Systems BT473) was used with EphAs or EphBs, respectively.

    Techniques: Binding Assay

    Inhibition of cell-retraction and rounding in PC3 cells by compound 20. A) Dose-response curve for compound 20 in presence of ephrin-A1-Fc (black bar), or Fc alone (white bar) as a control. The histogram shows the average percentage of retracting cells

    Journal: Journal of medicinal chemistry

    Article Title: Amino acid conjugates of lithocholic acid as antagonists of the EphA2 receptor

    doi: 10.1021/jm301890k

    Figure Lengend Snippet: Inhibition of cell-retraction and rounding in PC3 cells by compound 20. A) Dose-response curve for compound 20 in presence of ephrin-A1-Fc (black bar), or Fc alone (white bar) as a control. The histogram shows the average percentage of retracting cells

    Article Snippet: During the experiment to determine selectivity of compounds, all EphAs (R & D Systems SMPK1) and EphBs (R & D Systems SMPK2) receptors were incubated overnight similarly to EphA2 as previously described; 150 ng/ml biotinylated ephrin-A1-Fc or biotinylated ephrin-B1-Fc (R & D Systems BT473) was used with EphAs or EphBs, respectively.

    Techniques: Inhibition

    Relative EphA2 phosphorylation in the presence of different concentrations (50 μM, 25 μM, 12 μM, 6 μM) of compounds 1 (black), 2 (white) and 4 (sky blue). EphA2 phosphorylation was induced by treatment of PC3 cells with 0.25 μg/mL ephrin-A1-Fc. Cells were pretreated for 20 min with 1% DMSO or the indicated concentration of compounds and then stimulated for 20 min with ephrin-A1-Fc. Data are reported as a mean ± SEM of at least three independent experiments. One-way ANOVA followed by Dunnet’s post-test was performed to compare ephrin-A1-Fc + DMSO to all the other columns. * p

    Journal: Molecules

    Article Title: Synthesis and Structure-Activity Relationships of Amino Acid Conjugates of Cholanic Acid as Antagonists of the EphA2 Receptor

    doi: 10.3390/molecules181013043

    Figure Lengend Snippet: Relative EphA2 phosphorylation in the presence of different concentrations (50 μM, 25 μM, 12 μM, 6 μM) of compounds 1 (black), 2 (white) and 4 (sky blue). EphA2 phosphorylation was induced by treatment of PC3 cells with 0.25 μg/mL ephrin-A1-Fc. Cells were pretreated for 20 min with 1% DMSO or the indicated concentration of compounds and then stimulated for 20 min with ephrin-A1-Fc. Data are reported as a mean ± SEM of at least three independent experiments. One-way ANOVA followed by Dunnet’s post-test was performed to compare ephrin-A1-Fc + DMSO to all the other columns. * p

    Article Snippet: Compounds were added to the wells at proper concentration in 1% DMSO and incubated at 37 °C for 1 h. Biotinylated ephrin-A1-Fc (R & D Systems BT602) was added at 37 °C for 4 h at its KD in displacement assays.

    Techniques: Concentration Assay

    Effect of UniPR129 on HUVEC migration. (A) The bottom of the wells of the Boyden chamber was coated with ephrin-A1-Fc or with PBS for control. HUVEC treated with different concentrations of UniPR129 or DMSO were seeded on the top wells of the chamber. After 3 h at 37°C, non-migrated cells were gently removed from the membrane with a scraper, while migrated cells were fixed with formaldehyde and stained with DAPI. Photographs were taken in order to count the migrated cells in each well. The rate of migration for each group of chemotactic stimulus was normalized to the cells treated with DMSO (100% migration) in the absence of UniPR129. (B) Confluent HUVEC seeded on a 24-well plate were scratched with a pipette tip, washed with PBS and incubated for 15 h with different concentrations of UniPR129 or control (DMSO) in the presence or absence of 100 ng·mL −1 VEGF. Pictures were taken immediately after the scratch (T 0 ) and after 15 h (T 15 ). The wound area at T 0 and T 15 was measured with ImageJ software and wound healing percentage was calculated for each treatment. Dasatinib (Dasa;1 μM) was used as reference compound. Data are the means of at least three independent experiments ± SEM. * P

    Journal: British Journal of Pharmacology

    Article Title: UniPR129 is a competitive small molecule Eph-ephrin antagonist blocking in vitro angiogenesis at low micromolar concentrations

    doi: 10.1111/bph.12669

    Figure Lengend Snippet: Effect of UniPR129 on HUVEC migration. (A) The bottom of the wells of the Boyden chamber was coated with ephrin-A1-Fc or with PBS for control. HUVEC treated with different concentrations of UniPR129 or DMSO were seeded on the top wells of the chamber. After 3 h at 37°C, non-migrated cells were gently removed from the membrane with a scraper, while migrated cells were fixed with formaldehyde and stained with DAPI. Photographs were taken in order to count the migrated cells in each well. The rate of migration for each group of chemotactic stimulus was normalized to the cells treated with DMSO (100% migration) in the absence of UniPR129. (B) Confluent HUVEC seeded on a 24-well plate were scratched with a pipette tip, washed with PBS and incubated for 15 h with different concentrations of UniPR129 or control (DMSO) in the presence or absence of 100 ng·mL −1 VEGF. Pictures were taken immediately after the scratch (T 0 ) and after 15 h (T 15 ). The wound area at T 0 and T 15 was measured with ImageJ software and wound healing percentage was calculated for each treatment. Dasatinib (Dasa;1 μM) was used as reference compound. Data are the means of at least three independent experiments ± SEM. * P

    Article Snippet: Compounds were added to the wells at proper concentration in 1% DMSO and incubated at 37°C for 1 h. Biotinylated ephrin-A1-Fc (R & D Systems BT602, Minneapolis, MN, USA) was added at 37°C for 4 h at its K D in displacement assays or in a range from 1 to 2000 ng·mL−1 in saturation studies.

    Techniques: Migration, Staining, Transferring, Incubation, Software

    UniPR129 partially discriminates between A and B Eph receptor classes. UniPR129 dose dependently inhibited binding of biotinylated ephrin-A1-Fc or ephrin-B1-Fc to EphA or EphB receptor ectodomains respectively. Data are the means of at least three independent experiments ± SEM. IC 50 values are indicated at the bottom.

    Journal: British Journal of Pharmacology

    Article Title: UniPR129 is a competitive small molecule Eph-ephrin antagonist blocking in vitro angiogenesis at low micromolar concentrations

    doi: 10.1111/bph.12669

    Figure Lengend Snippet: UniPR129 partially discriminates between A and B Eph receptor classes. UniPR129 dose dependently inhibited binding of biotinylated ephrin-A1-Fc or ephrin-B1-Fc to EphA or EphB receptor ectodomains respectively. Data are the means of at least three independent experiments ± SEM. IC 50 values are indicated at the bottom.

    Article Snippet: Compounds were added to the wells at proper concentration in 1% DMSO and incubated at 37°C for 1 h. Biotinylated ephrin-A1-Fc (R & D Systems BT602, Minneapolis, MN, USA) was added at 37°C for 4 h at its K D in displacement assays or in a range from 1 to 2000 ng·mL−1 in saturation studies.

    Techniques: Binding Assay

    UniPR129 competitively displaces EphA2-ephrin-A1 binding. (A) UniPR129 dose-dependently displaced the binding of ephrin-A1-Fc from immobilized EphA2-Fc. (B) Binding of ephrin-A1-Fc to immobilized EphA2-Fc ectodomain in the presence of different concentrations of UniPR129. (C) The dissociation constants ( K D ) from the previous plot were used to calculate log (dose ratio –1) and to construct the Schild plot. pK i of UniPR129 was estimated by the intersection of the interpolated line with the X-axis. (D) EphA2-ephrin-A1 binding in the presence of 10 μM UniPR129 with or without washing three times with PBS before adding ephrin-A1-Fc. The histogram shows the means of at least three independent experiments ± SEM.

    Journal: British Journal of Pharmacology

    Article Title: UniPR129 is a competitive small molecule Eph-ephrin antagonist blocking in vitro angiogenesis at low micromolar concentrations

    doi: 10.1111/bph.12669

    Figure Lengend Snippet: UniPR129 competitively displaces EphA2-ephrin-A1 binding. (A) UniPR129 dose-dependently displaced the binding of ephrin-A1-Fc from immobilized EphA2-Fc. (B) Binding of ephrin-A1-Fc to immobilized EphA2-Fc ectodomain in the presence of different concentrations of UniPR129. (C) The dissociation constants ( K D ) from the previous plot were used to calculate log (dose ratio –1) and to construct the Schild plot. pK i of UniPR129 was estimated by the intersection of the interpolated line with the X-axis. (D) EphA2-ephrin-A1 binding in the presence of 10 μM UniPR129 with or without washing three times with PBS before adding ephrin-A1-Fc. The histogram shows the means of at least three independent experiments ± SEM.

    Article Snippet: Compounds were added to the wells at proper concentration in 1% DMSO and incubated at 37°C for 1 h. Biotinylated ephrin-A1-Fc (R & D Systems BT602, Minneapolis, MN, USA) was added at 37°C for 4 h at its K D in displacement assays or in a range from 1 to 2000 ng·mL−1 in saturation studies.

    Techniques: Binding Assay, Construct

    Binding interactions of ephrin Fc fusion proteins with Eph receptors. Apparent dissociation constant (K D ) values were obtained from curves measuring the binding of biotinylated ephrin Fc proteins to Eph receptor Fc proteins immobilized on ELISA plates.

    Journal: Cell Adhesion & Migration

    Article Title: Profiling Eph receptor expression in cells and tissues

    doi: 10.4161/cam.19620

    Figure Lengend Snippet: Binding interactions of ephrin Fc fusion proteins with Eph receptors. Apparent dissociation constant (K D ) values were obtained from curves measuring the binding of biotinylated ephrin Fc proteins to Eph receptor Fc proteins immobilized on ELISA plates.

    Article Snippet: After washing, the wells were incubated for 2 h with different concentrations of biotinylated ephrin Fc proteins (R & D Systems) in TBST (150 mM NaCl, 50 mM TRIS-HCl, pH 7.5 with 0.01% Tween 20) at room temperature, washed, and then incubated for 30 min with 0.5 μg/ml streptavidin conjugated to horseradish peroxidase (HRP) (Thermo Fisher Scientific, #21127) in TBST.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay