biotin conjugated goat anti rabbit igg secondary antibody  (Millipore)


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    Structured Review

    Millipore biotin conjugated goat anti rabbit igg secondary antibody
    Biotin Conjugated Goat Anti Rabbit Igg Secondary Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat anti rabbit igg secondary antibody/product/Millipore
    Average 88 stars, based on 136 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit igg secondary antibody - by Bioz Stars, 2020-02
    88/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Multiple embryonic origins of nitric oxide synthase-expressing GABAergic neurons of the neocortex
    Article Snippet: Paragraph title: Immunohistochemistry ... Secondary antibodies used were biotin-conjugated donkey anti-rabbit IgG (1:500; Millipore), biotin-conjugated donkey anti-sheep IgG (1:200; Thermo Scientific), AlexaFluor 488- and AlexaFluor 568-conjugated goat anti-rabbit IgG, or goat anti-rat IgG or goat anti-mouse IgG (all used at 1:750; Invitrogen).

    Article Title: PROX1: A Lineage Tracer for Cortical Interneurons Originating in the Lateral/Caudal Ganglionic Eminence and Preoptic Area
    Article Snippet: Immunohistochemistry was performed with the following primary antibodies: rabbit anti-Prox1 (1∶500; ReliaTech GmbH); goat anti-Prox1 (1∶500; R & D Systems, Inc.); rat anti-GFP IgG2a (1∶1000; Nacalai Tesque, Kyoto,Japan); rabbit anti-calretinin (1∶2000; Swant, Bellizona, Switzerland); sheep anti-neuropeptide Y (1∶500; Abcam); guinea pig anti-vasoactive intestinal peptide (1∶1000; Peninsula Laboratories, San Carlos, CA), mouse anti-parvalbumin (1∶1000; Swant), mouse anti-reelin (1∶2000; kindly provided by A.Goffinet); guinea pig anti-Sox10 (1∶4000; kindly provided by M. Wegner); mouse anti-ki67 (1∶1000, BD Pharmingen). .. For immunodetection of PROX1, biotin-conjugated donkey anti-rabbit IgG (1∶500; Millipore) or biotin-conjugated donkey anti-goat IgG (1∶200, Jackson Immunochemicals) secondary antibodies were applied for 1 hr at room temperature followed by Avidin/Biotinylated enzyme Complex (ABC) and Tyramide Signal Amplification (TSA) as described previously .

    Amplification:

    Article Title: Multiple embryonic origins of nitric oxide synthase-expressing GABAergic neurons of the neocortex
    Article Snippet: Tyramide-Cy3 (Perkin Elmer) (1:300 in amplification buffer) or DAB substrate reagent (Vector Laboratories) were applied for 3 min or 1 min, respectively, before sections were mounted. .. Secondary antibodies used were biotin-conjugated donkey anti-rabbit IgG (1:500; Millipore), biotin-conjugated donkey anti-sheep IgG (1:200; Thermo Scientific), AlexaFluor 488- and AlexaFluor 568-conjugated goat anti-rabbit IgG, or goat anti-rat IgG or goat anti-mouse IgG (all used at 1:750; Invitrogen).

    Article Title: PROX1: A Lineage Tracer for Cortical Interneurons Originating in the Lateral/Caudal Ganglionic Eminence and Preoptic Area
    Article Snippet: .. For immunodetection of PROX1, biotin-conjugated donkey anti-rabbit IgG (1∶500; Millipore) or biotin-conjugated donkey anti-goat IgG (1∶200, Jackson Immunochemicals) secondary antibodies were applied for 1 hr at room temperature followed by Avidin/Biotinylated enzyme Complex (ABC) and Tyramide Signal Amplification (TSA) as described previously . .. Tyramide-Cy3 (Perkin Elmer) was diluted at 1∶100 and the colour was developed for 10 min at room temperature.

    Fluorescence:

    Article Title: Neurochemical properties of BDNF-containing neurons projecting to rostral ventromedial medulla in the ventrolateral periaqueductal gray
    Article Snippet: Then the sections were subjected to the following sequential incubations with: (1) primary antibodies in the antibody dilution medium (0.01 M PBS containing 5% (v/v) NDS (PBS-NDS), 0.3% (v/v) Triton X-100, 0.05% (w/v) NaN3 and 0.25% (w/v) λ-carrageenan) overnight at RT and then 72 h at 4°C; (2) a mixture of secondary antibodies in PBS-NDS for 3 h at RT and then 10 h at 4°C; (3) fluorescence isothiocyanate (FITC)-conjugated avidin in PBS containing 0.3% Triton X-100 (PBS-X, pH 7.4), for 2 h at RT. .. The antibodies used in the current study were rabbit anti-BDNF antiserum (ab6201; Abcam, Cambridge, MA, USA); mouse anti-NeuN antiserum (MAB377; Millipore, Billerica, MA, USA); mouse anti-GFAP antiserum (MAB3402; Millipore); mouse anti-OX42 antiserum (CBL1512; Millipore); guinea pig anti-FG antiserum (NM-101; PROTOS BIOTECH CORP, New York, NY, USA); goat anti-5-HT antiserum (20079; ImmunoStar, Houston, Texas, USA); rat anti-NT antiserum (NP-103; PROTOS BIOTECH CORP); rat anti-SP antiserum (MAB356; Millipore); goat anti-CGRP antiserum (ab36001; Abcam); mouse anti-NOS antiserum (N-2280; Sigma, St. Louis, MO, USA); mouse anti-PV antiserum (P-3171; Sigma); mouse anti-TH antiserum (T-2928; Sigma); goat anti-TrkB antiserum (sc-20542; Santa Cruz Biotechnology, Santa Cruz, CA, USA); mouse anti-FOS antiserum (ab11959; Abcam); biotin-donkey anti-rabbit IgG (AP182F; Millipore); Alexa594-donkey anti-mouse IgG (A21203; Invitrogen, Carlsbad, CA, USA); Alexa594-goat anti-guinea pig IgG (A-11076; Invitrogen); Alexa594-donkey anti-goat IgG (A-11058; Invitrogen); Cy3-donkey anti-rat IgG (AP189c; Millipore); Alexa647-donkey anti-guinea pig IgG (AP193SA6; Millipore); FITC-Avidin (A-2001; Vector, Burlingame, CA, USA).

    Software:

    Article Title: Constitutive smooth muscle tumour necrosis factor regulates microvascular myogenic responsiveness and systemic blood pressure
    Article Snippet: Developed Kodak photographic films were scanned into a personal computer and densitometry was quantified using image analysis software (Image J, NIH) and expressed as % phosphorylated protein/total protein. .. Briefly, PVDF membranes were incubated overnight with rabbit anti-phospho-p44/42 MAPK (ERK1/2) antibody (1:1,000; Cat #4377S, Cell Signaling Technology) or rabbit anti-p44/42 MAPK (ERK1/2) antibody (1:1,000; Cat #4695S, Cell Signaling Technology), incubated with donkey anti-rabbit biotin IgG (1:20,000 Millipore), and then high sensitivity streptavidin-HRP (1:500, Pierce).

    Immunodetection:

    Article Title: PROX1: A Lineage Tracer for Cortical Interneurons Originating in the Lateral/Caudal Ganglionic Eminence and Preoptic Area
    Article Snippet: .. For immunodetection of PROX1, biotin-conjugated donkey anti-rabbit IgG (1∶500; Millipore) or biotin-conjugated donkey anti-goat IgG (1∶200, Jackson Immunochemicals) secondary antibodies were applied for 1 hr at room temperature followed by Avidin/Biotinylated enzyme Complex (ABC) and Tyramide Signal Amplification (TSA) as described previously . .. Tyramide-Cy3 (Perkin Elmer) was diluted at 1∶100 and the colour was developed for 10 min at room temperature.

    Labeling:

    Article Title: Tonotopic Gradients of Membrane and Synaptic Properties for Neurons of the Chicken Nucleus Magnocellularis
    Article Snippet: Sections were washed with PBS, incubated for 1 hr in an incubation buffer containing 1% BSA, 0.25% carrageenan (Sigma), 0.02% sodium azide, 0.3% Triton X-100 in PBS, incubated overnight at 4°C with antibody (1:200), washed with 0.3% Triton X-PBS, and then incubated in a 1:500 dilution of biotin-conjugated donkey anti-rabbit IgG (Chemicon, Temecula, CA) in the incubation buffer. .. DiI labeling.

    Avidin-Biotin Assay:

    Article Title: PROX1: A Lineage Tracer for Cortical Interneurons Originating in the Lateral/Caudal Ganglionic Eminence and Preoptic Area
    Article Snippet: .. For immunodetection of PROX1, biotin-conjugated donkey anti-rabbit IgG (1∶500; Millipore) or biotin-conjugated donkey anti-goat IgG (1∶200, Jackson Immunochemicals) secondary antibodies were applied for 1 hr at room temperature followed by Avidin/Biotinylated enzyme Complex (ABC) and Tyramide Signal Amplification (TSA) as described previously . .. Tyramide-Cy3 (Perkin Elmer) was diluted at 1∶100 and the colour was developed for 10 min at room temperature.

    Article Title: Neurochemical properties of BDNF-containing neurons projecting to rostral ventromedial medulla in the ventrolateral periaqueductal gray
    Article Snippet: Then the sections were subjected to the following sequential incubations with: (1) primary antibodies in the antibody dilution medium (0.01 M PBS containing 5% (v/v) NDS (PBS-NDS), 0.3% (v/v) Triton X-100, 0.05% (w/v) NaN3 and 0.25% (w/v) λ-carrageenan) overnight at RT and then 72 h at 4°C; (2) a mixture of secondary antibodies in PBS-NDS for 3 h at RT and then 10 h at 4°C; (3) fluorescence isothiocyanate (FITC)-conjugated avidin in PBS containing 0.3% Triton X-100 (PBS-X, pH 7.4), for 2 h at RT. .. The antibodies used in the current study were rabbit anti-BDNF antiserum (ab6201; Abcam, Cambridge, MA, USA); mouse anti-NeuN antiserum (MAB377; Millipore, Billerica, MA, USA); mouse anti-GFAP antiserum (MAB3402; Millipore); mouse anti-OX42 antiserum (CBL1512; Millipore); guinea pig anti-FG antiserum (NM-101; PROTOS BIOTECH CORP, New York, NY, USA); goat anti-5-HT antiserum (20079; ImmunoStar, Houston, Texas, USA); rat anti-NT antiserum (NP-103; PROTOS BIOTECH CORP); rat anti-SP antiserum (MAB356; Millipore); goat anti-CGRP antiserum (ab36001; Abcam); mouse anti-NOS antiserum (N-2280; Sigma, St. Louis, MO, USA); mouse anti-PV antiserum (P-3171; Sigma); mouse anti-TH antiserum (T-2928; Sigma); goat anti-TrkB antiserum (sc-20542; Santa Cruz Biotechnology, Santa Cruz, CA, USA); mouse anti-FOS antiserum (ab11959; Abcam); biotin-donkey anti-rabbit IgG (AP182F; Millipore); Alexa594-donkey anti-mouse IgG (A21203; Invitrogen, Carlsbad, CA, USA); Alexa594-goat anti-guinea pig IgG (A-11076; Invitrogen); Alexa594-donkey anti-goat IgG (A-11058; Invitrogen); Cy3-donkey anti-rat IgG (AP189c; Millipore); Alexa647-donkey anti-guinea pig IgG (AP193SA6; Millipore); FITC-Avidin (A-2001; Vector, Burlingame, CA, USA).

    Incubation:

    Article Title: Constitutive smooth muscle tumour necrosis factor regulates microvascular myogenic responsiveness and systemic blood pressure
    Article Snippet: .. Briefly, PVDF membranes were incubated overnight with rabbit anti-phospho-p44/42 MAPK (ERK1/2) antibody (1:1,000; Cat #4377S, Cell Signaling Technology) or rabbit anti-p44/42 MAPK (ERK1/2) antibody (1:1,000; Cat #4695S, Cell Signaling Technology), incubated with donkey anti-rabbit biotin IgG (1:20,000 Millipore), and then high sensitivity streptavidin-HRP (1:500, Pierce). ..

    Article Title: Tonotopic Gradients of Membrane and Synaptic Properties for Neurons of the Chicken Nucleus Magnocellularis
    Article Snippet: .. Sections were washed with PBS, incubated for 1 hr in an incubation buffer containing 1% BSA, 0.25% carrageenan (Sigma), 0.02% sodium azide, 0.3% Triton X-100 in PBS, incubated overnight at 4°C with antibody (1:200), washed with 0.3% Triton X-PBS, and then incubated in a 1:500 dilution of biotin-conjugated donkey anti-rabbit IgG (Chemicon, Temecula, CA) in the incubation buffer. .. After washing with 0.3% Triton X-PBS, slices were incubated in avidinhorseradish peroxidase ABC solution (Vector Laboratories, Burlingame, CA) for 2 hr, reacted with a 3, 3′-diaminobenzidine (DAB; Wako) solution containing 0.2% DAB, 0.02% NiCl2 , 0.001% H2 O2 , and 50 m m Tris, pH 7.4.

    other:

    Article Title: Effects of Lesions of the Histaminergic Tuberomammillary Nucleus on Spontaneous Sleep in Rats
    Article Snippet: The following secondary antibodies were used: biotin-conjugated donkey anti-rabbit IgG (1:500, Cat. No. AP182B; Chemicon) and biotin-conjugated donkey anti-mouse IgG (1:500, Cat. No. AP192B; Chemicon).

    Staining:

    Article Title: PROX1: A Lineage Tracer for Cortical Interneurons Originating in the Lateral/Caudal Ganglionic Eminence and Preoptic Area
    Article Snippet: Adult sections were cut coronally (30 µm thickness) and were collected in PBS for “free floating” staining. .. For immunodetection of PROX1, biotin-conjugated donkey anti-rabbit IgG (1∶500; Millipore) or biotin-conjugated donkey anti-goat IgG (1∶200, Jackson Immunochemicals) secondary antibodies were applied for 1 hr at room temperature followed by Avidin/Biotinylated enzyme Complex (ABC) and Tyramide Signal Amplification (TSA) as described previously .

    Article Title: Tonotopic Gradients of Membrane and Synaptic Properties for Neurons of the Chicken Nucleus Magnocellularis
    Article Snippet: The staining pattern by this antibody was similar to that in the study by Lu et al. ( ). .. Sections were washed with PBS, incubated for 1 hr in an incubation buffer containing 1% BSA, 0.25% carrageenan (Sigma), 0.02% sodium azide, 0.3% Triton X-100 in PBS, incubated overnight at 4°C with antibody (1:200), washed with 0.3% Triton X-PBS, and then incubated in a 1:500 dilution of biotin-conjugated donkey anti-rabbit IgG (Chemicon, Temecula, CA) in the incubation buffer.

    Modification:

    Article Title: Constitutive smooth muscle tumour necrosis factor regulates microvascular myogenic responsiveness and systemic blood pressure
    Article Snippet: Western blots involving sTNFR1-Fc in wild-type mouse vessels, and all Sm-TNF-KO experiments, used a modified method that improved sensitivity and thereby reduced tissue requirements. .. Briefly, PVDF membranes were incubated overnight with rabbit anti-phospho-p44/42 MAPK (ERK1/2) antibody (1:1,000; Cat #4377S, Cell Signaling Technology) or rabbit anti-p44/42 MAPK (ERK1/2) antibody (1:1,000; Cat #4695S, Cell Signaling Technology), incubated with donkey anti-rabbit biotin IgG (1:20,000 Millipore), and then high sensitivity streptavidin-HRP (1:500, Pierce).

    Western Blot:

    Article Title: Constitutive smooth muscle tumour necrosis factor regulates microvascular myogenic responsiveness and systemic blood pressure
    Article Snippet: Paragraph title: Western blotting ... Briefly, PVDF membranes were incubated overnight with rabbit anti-phospho-p44/42 MAPK (ERK1/2) antibody (1:1,000; Cat #4377S, Cell Signaling Technology) or rabbit anti-p44/42 MAPK (ERK1/2) antibody (1:1,000; Cat #4695S, Cell Signaling Technology), incubated with donkey anti-rabbit biotin IgG (1:20,000 Millipore), and then high sensitivity streptavidin-HRP (1:500, Pierce).

    Recombinant:

    Article Title: Multiple embryonic origins of nitric oxide synthase-expressing GABAergic neurons of the neocortex
    Article Snippet: These included the following: rabbit anti-nNOS that recognizes 195 amino acids from N-terminus of the rat nNOS (1:500; Invitrogen; Cat no. 61-7000), sheep anti-nNOS generated against recombinant rat nNOS [1:1000; (kindly provided by P. Emson) (Herbison et al., )], mouse monoclonal anti-nNOS that recognizes amino acids 1095–1289 from the C terminus of human nNOS (1:200; BD Biosciences; Cat no. N31020-050), and rabbit anti-nNOS generated against amino acids 1419–1433 from the C terminus of human nNOS (1:1000; Immunostar; Cat no. 24287). .. Secondary antibodies used were biotin-conjugated donkey anti-rabbit IgG (1:500; Millipore), biotin-conjugated donkey anti-sheep IgG (1:200; Thermo Scientific), AlexaFluor 488- and AlexaFluor 568-conjugated goat anti-rabbit IgG, or goat anti-rat IgG or goat anti-mouse IgG (all used at 1:750; Invitrogen).

    Immunofluorescence:

    Article Title: Neurochemical properties of BDNF-containing neurons projecting to rostral ventromedial medulla in the ventrolateral periaqueductal gray
    Article Snippet: Paragraph title: Double- or triple-labeling immunofluorescence (IF) histochemistry ... The antibodies used in the current study were rabbit anti-BDNF antiserum (ab6201; Abcam, Cambridge, MA, USA); mouse anti-NeuN antiserum (MAB377; Millipore, Billerica, MA, USA); mouse anti-GFAP antiserum (MAB3402; Millipore); mouse anti-OX42 antiserum (CBL1512; Millipore); guinea pig anti-FG antiserum (NM-101; PROTOS BIOTECH CORP, New York, NY, USA); goat anti-5-HT antiserum (20079; ImmunoStar, Houston, Texas, USA); rat anti-NT antiserum (NP-103; PROTOS BIOTECH CORP); rat anti-SP antiserum (MAB356; Millipore); goat anti-CGRP antiserum (ab36001; Abcam); mouse anti-NOS antiserum (N-2280; Sigma, St. Louis, MO, USA); mouse anti-PV antiserum (P-3171; Sigma); mouse anti-TH antiserum (T-2928; Sigma); goat anti-TrkB antiserum (sc-20542; Santa Cruz Biotechnology, Santa Cruz, CA, USA); mouse anti-FOS antiserum (ab11959; Abcam); biotin-donkey anti-rabbit IgG (AP182F; Millipore); Alexa594-donkey anti-mouse IgG (A21203; Invitrogen, Carlsbad, CA, USA); Alexa594-goat anti-guinea pig IgG (A-11076; Invitrogen); Alexa594-donkey anti-goat IgG (A-11058; Invitrogen); Cy3-donkey anti-rat IgG (AP189c; Millipore); Alexa647-donkey anti-guinea pig IgG (AP193SA6; Millipore); FITC-Avidin (A-2001; Vector, Burlingame, CA, USA).

    Plasmid Preparation:

    Article Title: Tonotopic Gradients of Membrane and Synaptic Properties for Neurons of the Chicken Nucleus Magnocellularis
    Article Snippet: Sections were washed with PBS, incubated for 1 hr in an incubation buffer containing 1% BSA, 0.25% carrageenan (Sigma), 0.02% sodium azide, 0.3% Triton X-100 in PBS, incubated overnight at 4°C with antibody (1:200), washed with 0.3% Triton X-PBS, and then incubated in a 1:500 dilution of biotin-conjugated donkey anti-rabbit IgG (Chemicon, Temecula, CA) in the incubation buffer. .. After washing with 0.3% Triton X-PBS, slices were incubated in avidinhorseradish peroxidase ABC solution (Vector Laboratories, Burlingame, CA) for 2 hr, reacted with a 3, 3′-diaminobenzidine (DAB; Wako) solution containing 0.2% DAB, 0.02% NiCl2 , 0.001% H2 O2 , and 50 m m Tris, pH 7.4.

    Generated:

    Article Title: Multiple embryonic origins of nitric oxide synthase-expressing GABAergic neurons of the neocortex
    Article Snippet: Data presented in this study were generated using the rabbit anti-nNOS (Immunostar) and the sheep anti-nNOS (Herbison et al., ). .. Secondary antibodies used were biotin-conjugated donkey anti-rabbit IgG (1:500; Millipore), biotin-conjugated donkey anti-sheep IgG (1:200; Thermo Scientific), AlexaFluor 488- and AlexaFluor 568-conjugated goat anti-rabbit IgG, or goat anti-rat IgG or goat anti-mouse IgG (all used at 1:750; Invitrogen).

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  • 80
    Millipore biotin conjugated donkey anti rabbit igg
    Biotin Conjugated Donkey Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated donkey anti rabbit igg/product/Millipore
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated donkey anti rabbit igg - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    88
    Millipore biotin conjugated goat anti rabbit igg secondary antibody
    Biotin Conjugated Goat Anti Rabbit Igg Secondary Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat anti rabbit igg secondary antibody/product/Millipore
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit igg secondary antibody - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    78
    Millipore biotin conjugated goat anti rabbit igg pab
    Biotin Conjugated Goat Anti Rabbit Igg Pab, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat anti rabbit igg pab/product/Millipore
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit igg pab - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

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