biotin sp conjugated affinipure f  (Jackson Immuno)

 
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  • 92
    Name:
    Biotin SP AffiniPure Goat Anti Human IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule human IgG It also reacts with the light chains of other human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    109-065-003
    Price:
    104
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    2 0
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    Structured Review

    Jackson Immuno biotin sp conjugated affinipure f
    Biotin SP AffiniPure Goat Anti Human IgG
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule human IgG It also reacts with the light chains of other human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp conjugated affinipure f/product/Jackson Immuno
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipure f - by Bioz Stars, 2020-07
    92/100 stars

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    Related Articles

    Staining:

    Article Title: Intrinsic defects in B cell response to seasonal influenza vaccination in elderly humans
    Article Snippet: .. To detect naive B cells (IgG− /IgA− /CD27− ), IgM memory B cells (IgG− /IgA− /CD27+ ), and switch memory B cells (IgG+ /IgA+ ), blood cells were stained with PE-conjugated anti-CD27 (BD Pharmingen 555441), biotin-SP-conjugated AffiniPure F(ab′)2 fragment anti-human IgG (Jackson ImmunoResearch Laboratories.109-066-098), and biotin-SP-conjugated AffiniPure F(ab′)2 fragment anti-human IgA (Jackson ImmunoResearch Laboratories no.109-066-011). .. After washings, biotin-conjugated antibodies were revealed with PerCP-conjugated streptavidin (1/40 diluted; BD Pharmingen 554067).

    Amplification:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: .. After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch). .. Live cells (∼60%) were sorted by propidium iodide exclusion and analyzed on a FACSCalibur machine (BD Biosciences).

    FACS:

    Article Title: Hedgehog Pathway Antagonist 5E1 Binds Hedgehog at the Pseudo-active Site
    Article Snippet: .. After two washes in FACS buffer, the 5E1 signal was amplified with 1:100 biotinylated goat anti-mouse for m5E1 (catalog no. 115-065-071, Jackson ImmunoResearch) or anti-human for ch5E1 (catalog no. 109-065-003, Jackson ImmunoResearch) for 1 h prior to two washes and detection with 1:50 rhodamine streptavidin-phycoerythrin (catalog no. 016-110-084, Jackson ImmunoResearch). .. Live cells (∼60%) were sorted by propidium iodide exclusion and analyzed on a FACSCalibur machine (BD Biosciences).

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  • 94
    Jackson Immuno biotin sp conjugated affinipure f
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