biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Goat Anti Mouse IgG Fcγ Subclass 2c Specific
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on antigen binding assay and or ELISA the antibody reacts with the Fc portion of mouse IgG2c but not with other mouse IgG subclasses mouse IgM or the Fab portion of mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human bovine and rabbit serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    115-065-208
    Price:
    207
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    0 5
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    Structured Review

    Jackson Immuno biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific
    Biotin SP AffiniPure Goat Anti Mouse IgG Fcγ Subclass 2c Specific
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on antigen binding assay and or ELISA the antibody reacts with the Fc portion of mouse IgG2c but not with other mouse IgG subclasses mouse IgM or the Fab portion of mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human bovine and rabbit serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Mouse Assay:

    Article Title: B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction
    Article Snippet: Circulating IgM, IgG1, and IgG2c levels were measured in serum of treated mice using a chemiluminescence-based sandwich ELISA and matched pairs of specific antibodies for capture and detection. .. The following antibodies were paired with appropriate detecting antibodies: goat anti-mouse IgM (Sigma-Aldrich; M8644) with alkaline phosphatase (AP)-labeled goat anti-mouse IgM (Sigma-Aldrich; A 9688); rat anti-mouse IgG1 (Biolegend; 406602) with biotinylated rat anti-mouse IgG1 (BD; 553441); and goat anti-mouse IgG2c (AbD Serotec; STAR135) with biotinylated goat anti-mouse IgG2c (Jackson ImmunoResearch; 115-065-208).

    Enzyme-linked Immunosorbent Assay:

    Article Title: B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction
    Article Snippet: The following antibodies were paired with appropriate detecting antibodies: goat anti-mouse IgM (Sigma-Aldrich; M8644) with alkaline phosphatase (AP)-labeled goat anti-mouse IgM (Sigma-Aldrich; A 9688); rat anti-mouse IgG1 (Biolegend; 406602) with biotinylated rat anti-mouse IgG1 (BD; 553441); and goat anti-mouse IgG2c (AbD Serotec; STAR135) with biotinylated goat anti-mouse IgG2c (Jackson ImmunoResearch; 115-065-208). .. All ELISA assays were developed using LumiPhos Plus (Lumigen).

    Sandwich ELISA:

    Article Title: B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction
    Article Snippet: Circulating IgM, IgG1, and IgG2c levels were measured in serum of treated mice using a chemiluminescence-based sandwich ELISA and matched pairs of specific antibodies for capture and detection. .. The following antibodies were paired with appropriate detecting antibodies: goat anti-mouse IgM (Sigma-Aldrich; M8644) with alkaline phosphatase (AP)-labeled goat anti-mouse IgM (Sigma-Aldrich; A 9688); rat anti-mouse IgG1 (Biolegend; 406602) with biotinylated rat anti-mouse IgG1 (BD; 553441); and goat anti-mouse IgG2c (AbD Serotec; STAR135) with biotinylated goat anti-mouse IgG2c (Jackson ImmunoResearch; 115-065-208).

    Incubation:

    Article Title: B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction
    Article Snippet: The following antibodies were paired with appropriate detecting antibodies: goat anti-mouse IgM (Sigma-Aldrich; M8644) with alkaline phosphatase (AP)-labeled goat anti-mouse IgM (Sigma-Aldrich; A 9688); rat anti-mouse IgG1 (Biolegend; 406602) with biotinylated rat anti-mouse IgG1 (BD; 553441); and goat anti-mouse IgG2c (AbD Serotec; STAR135) with biotinylated goat anti-mouse IgG2c (Jackson ImmunoResearch; 115-065-208). .. An extra incubation step with AP-labeled NeutrAvidin (Pierce) was performed in assays in which biotinylated detection antibodies were used.

    Purification:

    Article Title: B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction
    Article Snippet: The following antibodies were paired with appropriate detecting antibodies: goat anti-mouse IgM (Sigma-Aldrich; M8644) with alkaline phosphatase (AP)-labeled goat anti-mouse IgM (Sigma-Aldrich; A 9688); rat anti-mouse IgG1 (Biolegend; 406602) with biotinylated rat anti-mouse IgG1 (BD; 553441); and goat anti-mouse IgG2c (AbD Serotec; STAR135) with biotinylated goat anti-mouse IgG2c (Jackson ImmunoResearch; 115-065-208). .. Purified immunoglobulin isotypes used for standardization were purchased from Biolegend or Southern Biotech.

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  • 94
    Jackson Immuno biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific
    Biotin Sp Affinipure Goat Anti Mouse Igg Fcγ Subclass 2c Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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