biotin sp affinipure goat anti human iga igg igm  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Goat Anti Human IgA IgG IgM
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with human IgA IgG and IgM It also reacts with the light chains of other human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    109-065-064
    Price:
    141
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    2 0
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    Structured Review

    Jackson Immuno biotin sp affinipure goat anti human iga igg igm
    Biotin SP AffiniPure Goat Anti Human IgA IgG IgM
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with human IgA IgG and IgM It also reacts with the light chains of other human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure goat anti human iga igg igm/product/Jackson Immuno
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti human iga igg igm - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    antigen-loaded beads

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    Related Articles

    Multiplex Assay:

    Article Title: Validation of Multiplex Serology detecting human herpesviruses 1-5
    Article Snippet: Paragraph title: Monoplex and Multiplex Serology ... Detection of bound primary antibodies from serum took place with a biotinlyated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA) and subsequent incubation with streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA) as reporter dye.

    Article Title: Validation of Multiplex Serology for human hepatitis viruses B and C, human T-lymphotropic virus 1 and Toxoplasma gondii
    Article Snippet: Paragraph title: Monoplex and Multiplex Serology ... Serum was incubated with the antigen-loaded beads and primary antibodies were detected using a biotinylated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA).

    In Situ:

    Article Title: Validation of Multiplex Serology detecting human herpesviruses 1-5
    Article Snippet: Briefly, HHV GST-tag fusion proteins were loaded onto glutathione casein-coated fluorescence-labelled polystyrene beads (COOH-beads xMAP Technology Microspheres, Luminex Corp. Austin, Texas, USA) by in situ affinity purification from lysate. .. Detection of bound primary antibodies from serum took place with a biotinlyated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA) and subsequent incubation with streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA) as reporter dye.

    Fluorescence:

    Article Title: Validation of Multiplex Serology detecting human herpesviruses 1-5
    Article Snippet: Briefly, HHV GST-tag fusion proteins were loaded onto glutathione casein-coated fluorescence-labelled polystyrene beads (COOH-beads xMAP Technology Microspheres, Luminex Corp. Austin, Texas, USA) by in situ affinity purification from lysate. .. Detection of bound primary antibodies from serum took place with a biotinlyated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA) and subsequent incubation with streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA) as reporter dye.

    Article Title: Validation of Multiplex Serology for human hepatitis viruses B and C, human T-lymphotropic virus 1 and Toxoplasma gondii
    Article Snippet: Serum was incubated with the antigen-loaded beads and primary antibodies were detected using a biotinylated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA). .. Measuring at least 100 beads per bead set, Median Fluorescence Intensities (MFI) were calculated.

    Cytometry:

    Article Title: Validation of Multiplex Serology detecting human herpesviruses 1-5
    Article Snippet: Up to 100 bead sets are distinguishable by the Luminex flow cytometer via different ratios of two fluorescent dyes within the polystyrene microspheres. .. Detection of bound primary antibodies from serum took place with a biotinlyated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA) and subsequent incubation with streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA) as reporter dye.

    Article Title: Validation of Multiplex Serology for human hepatitis viruses B and C, human T-lymphotropic virus 1 and Toxoplasma gondii
    Article Snippet: Thus, up to 100 different bead sets can be distinguished by the Luminex flow cytometer. .. Serum was incubated with the antigen-loaded beads and primary antibodies were detected using a biotinylated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA).

    Flow Cytometry:

    Article Title: Validation of Multiplex Serology detecting human herpesviruses 1-5
    Article Snippet: Up to 100 bead sets are distinguishable by the Luminex flow cytometer via different ratios of two fluorescent dyes within the polystyrene microspheres. .. Detection of bound primary antibodies from serum took place with a biotinlyated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA) and subsequent incubation with streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA) as reporter dye.

    Article Title: Validation of Multiplex Serology for human hepatitis viruses B and C, human T-lymphotropic virus 1 and Toxoplasma gondii
    Article Snippet: Thus, up to 100 different bead sets can be distinguished by the Luminex flow cytometer. .. Serum was incubated with the antigen-loaded beads and primary antibodies were detected using a biotinylated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA).

    Incubation:

    Article Title: Validation of Multiplex Serology detecting human herpesviruses 1-5
    Article Snippet: .. Detection of bound primary antibodies from serum took place with a biotinlyated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA) and subsequent incubation with streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA) as reporter dye. .. Median Fluorescence Intensities (MFI) from at least 100 detected beads per bead set (e.g. antigen) were calculated for each serum.

    Article Title: Validation of Multiplex Serology for human hepatitis viruses B and C, human T-lymphotropic virus 1 and Toxoplasma gondii
    Article Snippet: .. Serum was incubated with the antigen-loaded beads and primary antibodies were detected using a biotinylated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA). .. Subsequent incubation with a reporter dye, streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA), allowed quantitative measurement of the formed immunocomplexes in a Luminex 200 flow cytometer.

    Affinity Purification:

    Article Title: Validation of Multiplex Serology detecting human herpesviruses 1-5
    Article Snippet: Briefly, HHV GST-tag fusion proteins were loaded onto glutathione casein-coated fluorescence-labelled polystyrene beads (COOH-beads xMAP Technology Microspheres, Luminex Corp. Austin, Texas, USA) by in situ affinity purification from lysate. .. Detection of bound primary antibodies from serum took place with a biotinlyated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA) and subsequent incubation with streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA) as reporter dye.

    Luminex:

    Article Title: Validation of Multiplex Serology for human hepatitis viruses B and C, human T-lymphotropic virus 1 and Toxoplasma gondii
    Article Snippet: Serum was incubated with the antigen-loaded beads and primary antibodies were detected using a biotinylated goat-α-human IgM/IgG/IgA secondary antibody (1:1000, #109-065-064, Jackson Immunoresearch, West Grove, PA, USA). .. Subsequent incubation with a reporter dye, streptavidin-R-phycoerythrin (1:750, PE-Streptavidin Conjugate, MOSS Inc., Pasadena, CA, USA), allowed quantitative measurement of the formed immunocomplexes in a Luminex 200 flow cytometer.

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  • About
  • News
  • Press Release
  • Team
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  • Contact
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  • 99
    Jackson Immuno biotin sp affinipure goat anti human iga igg igm
    Biotin Sp Affinipure Goat Anti Human Iga Igg Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti human iga igg igm/product/Jackson Immuno
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti human iga igg igm - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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