biotin sp affinipure goat anti horseradish peroxidase  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Goat Anti Horseradish Peroxidase
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with peroxidase from horseradish roots It may cross react with peroxidase from other sources
    Catalog Number:
    123-065-021
    Price:
    121
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    2 0
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure goat anti horseradish peroxidase
    Biotin SP AffiniPure Goat Anti Horseradish Peroxidase
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with peroxidase from horseradish roots It may cross react with peroxidase from other sources
    https://www.bioz.com/result/biotin sp affinipure goat anti horseradish peroxidase/product/Jackson Immuno
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti horseradish peroxidase - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: The increase of microRNA-21 during lung fibrosis and its contribution to epithelial-mesenchymal transition in pulmonary epithelial cells
    Article Snippet: Slides were washed in SSC buffers and incubated with blocking solution containing goat serum for 15 min at RT. .. Slides were then washed and incubated with biotin-SP-conjugated AffiniPure goat anti-horseradish peroxidase (1:200) at 37°C for 30 min (Jackson ImmunoResearch).

    Incubation:

    Article Title: LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3
    Article Snippet: .. Slides were then washed and incubated for horseradish peroxidase-conjugated streptavidin (123-065-021; Jackson ImmunoResearch) for 2 hours at room temperature. .. Peroxidase activity was detected with the addition of diaminobenzidine (D4293; Sigma) and 0.1% H2 O2 .

    Article Title: New Features on the Expression and Trafficking of mGluR1 Splice Variants Exposed by Two Novel Mutant Mouse Lines
    Article Snippet: The suspension was incubated at 4°C for 30 min and centrifuged at 20,000× g for 5 min. For immunoblot analysis, the supernatant was quantitated by the Bradford method (Coomassie PlusProtein Assay Reagent, Thermo Fisher Scientific) and denatured at 95°C for 5 min. .. Antigen-antibody complexes were detected with secondary antibodies conjugated with HRP (Jackson Immunoresearch, cat. no. 123-065-021, RRID:AB_2314646 ) and visualized by enhanced chemiluminescence (Amersham Hyperfilm ECL, GE Healthcare).

    Article Title: The increase of microRNA-21 during lung fibrosis and its contribution to epithelial-mesenchymal transition in pulmonary epithelial cells
    Article Snippet: .. Slides were then washed and incubated with biotin-SP-conjugated AffiniPure goat anti-horseradish peroxidase (1:200) at 37°C for 30 min (Jackson ImmunoResearch). .. Finally, the slides were washed and labeled with Streptavidin-AP (Roche).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Overexpression of Sonic Hedgehog in the Lung Mimics the Effect of Lung Injury and Compensatory Lung Growth on Pulmonary Sca-1 and CD34 Positive Cells
    Article Snippet: .. Formalin-fixed, paraffin-embedded slices were stained with rabbit anti-Ki67 antigen (Vector Labs, Burlingame, CA) followed by peroxidase-conjugated goat anti-rabbit (Jackson ImmunoResearch), biotin-conjugated goat anti–horseradish peroxidase (Jackson ImmunoResearch), and fluorescein isothiocyanate–conjugated streptavidin (Jackson ImmunoResearch). ..

    Activity Assay:

    Article Title: LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3
    Article Snippet: Slides were then washed and incubated for horseradish peroxidase-conjugated streptavidin (123-065-021; Jackson ImmunoResearch) for 2 hours at room temperature. .. Peroxidase activity was detected with the addition of diaminobenzidine (D4293; Sigma) and 0.1% H2 O2 .

    Article Title: LIM homeodomain transcription factor Isl1 affects urethral epithelium differentiation and apoptosis via Shh
    Article Snippet: .. A biotinylated goat anti-mouse secondary antibody (1:200; 115-065-146; Jackson ImmunoResearch, West Grove, PA, USA) was applied for 2 h at room temperature followed by streptavidin peroxidase (1:200; 123-065-021; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h. Diaminobenzidine (D4293; Sigma, St. Louis, MO, USA) and 0.1% H2 O2 were used for detecting peroxidase activity. .. Detection of proliferation and apoptotic cells To detect the proliferation of urethral cells, pregnant mice were injected intraperitoneally with BrdU (100 mg/kg body weight) 4 h before sacrifice.

    Hybridization:

    Article Title: The increase of microRNA-21 during lung fibrosis and its contribution to epithelial-mesenchymal transition in pulmonary epithelial cells
    Article Snippet: The hybridization mixture contained 5 nM double-DIG LNA™ microRNA probe for miR-21 or scramble-miR as the negative control (Exiqon) and was applied and hybridized for 1 h at 53°C. .. Slides were then washed and incubated with biotin-SP-conjugated AffiniPure goat anti-horseradish peroxidase (1:200) at 37°C for 30 min (Jackson ImmunoResearch).

    Immunohistochemistry:

    Article Title: LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3
    Article Snippet: Paragraph title: Immunohistochemistry ... Slides were then washed and incubated for horseradish peroxidase-conjugated streptavidin (123-065-021; Jackson ImmunoResearch) for 2 hours at room temperature.

    Article Title: LIM homeodomain transcription factor Isl1 affects urethral epithelium differentiation and apoptosis via Shh
    Article Snippet: Paragraph title: Immunofluorescence and immunohistochemistry ... A biotinylated goat anti-mouse secondary antibody (1:200; 115-065-146; Jackson ImmunoResearch, West Grove, PA, USA) was applied for 2 h at room temperature followed by streptavidin peroxidase (1:200; 123-065-021; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h. Diaminobenzidine (D4293; Sigma, St. Louis, MO, USA) and 0.1% H2 O2 were used for detecting peroxidase activity.

    Protease Inhibitor:

    Article Title: New Features on the Expression and Trafficking of mGluR1 Splice Variants Exposed by Two Novel Mutant Mouse Lines
    Article Snippet: Immunoblot Analysis and Co-immunoprecipitation Mouse cerebella were isolated and homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1% Triton X-100, protease inhibitor mixture (Complete EDTA-free, Roche) and phosphatase inhibitor cocktail mixture (PhosStop, Roche). .. Antigen-antibody complexes were detected with secondary antibodies conjugated with HRP (Jackson Immunoresearch, cat. no. 123-065-021, RRID:AB_2314646 ) and visualized by enhanced chemiluminescence (Amersham Hyperfilm ECL, GE Healthcare).

    SDS Page:

    Article Title: New Features on the Expression and Trafficking of mGluR1 Splice Variants Exposed by Two Novel Mutant Mouse Lines
    Article Snippet: The protein extracts were resolved by SDS-PAGE and transferred to a PVDF membrane (Immobilon-P, Millipore). .. Antigen-antibody complexes were detected with secondary antibodies conjugated with HRP (Jackson Immunoresearch, cat. no. 123-065-021, RRID:AB_2314646 ) and visualized by enhanced chemiluminescence (Amersham Hyperfilm ECL, GE Healthcare).

    Imaging:

    Article Title: LIM homeodomain transcription factor Isl1 affects urethral epithelium differentiation and apoptosis via Shh
    Article Snippet: Leica Microsystems was used for imaging immunofluorescent sections. .. A biotinylated goat anti-mouse secondary antibody (1:200; 115-065-146; Jackson ImmunoResearch, West Grove, PA, USA) was applied for 2 h at room temperature followed by streptavidin peroxidase (1:200; 123-065-021; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h. Diaminobenzidine (D4293; Sigma, St. Louis, MO, USA) and 0.1% H2 O2 were used for detecting peroxidase activity.

    Immunofluorescence:

    Article Title: LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3
    Article Snippet: Immunohistochemistry Paraffin sections were processed as described above (see Immunofluorescence). .. Slides were then washed and incubated for horseradish peroxidase-conjugated streptavidin (123-065-021; Jackson ImmunoResearch) for 2 hours at room temperature.

    Article Title: LIM homeodomain transcription factor Isl1 affects urethral epithelium differentiation and apoptosis via Shh
    Article Snippet: Paragraph title: Immunofluorescence and immunohistochemistry ... A biotinylated goat anti-mouse secondary antibody (1:200; 115-065-146; Jackson ImmunoResearch, West Grove, PA, USA) was applied for 2 h at room temperature followed by streptavidin peroxidase (1:200; 123-065-021; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h. Diaminobenzidine (D4293; Sigma, St. Louis, MO, USA) and 0.1% H2 O2 were used for detecting peroxidase activity.

    Article Title: Overexpression of Sonic Hedgehog in the Lung Mimics the Effect of Lung Injury and Compensatory Lung Growth on Pulmonary Sca-1 and CD34 Positive Cells
    Article Snippet: Quantification of CD34 and CC10 double-positive cells was performed by dual immunofluorescence by randomly counting 30 fields per mouse ( n = 5) the BADJ or whole lung. .. Formalin-fixed, paraffin-embedded slices were stained with rabbit anti-Ki67 antigen (Vector Labs, Burlingame, CA) followed by peroxidase-conjugated goat anti-rabbit (Jackson ImmunoResearch), biotin-conjugated goat anti–horseradish peroxidase (Jackson ImmunoResearch), and fluorescein isothiocyanate–conjugated streptavidin (Jackson ImmunoResearch).

    Magnetic Beads:

    Article Title: Overexpression of Sonic Hedgehog in the Lung Mimics the Effect of Lung Injury and Compensatory Lung Growth on Pulmonary Sca-1 and CD34 Positive Cells
    Article Snippet: Formalin-fixed, paraffin-embedded slices were stained with rabbit anti-Ki67 antigen (Vector Labs, Burlingame, CA) followed by peroxidase-conjugated goat anti-rabbit (Jackson ImmunoResearch), biotin-conjugated goat anti–horseradish peroxidase (Jackson ImmunoResearch), and fluorescein isothiocyanate–conjugated streptavidin (Jackson ImmunoResearch). .. Sca-1+ , CD45− , Pecam− coexpressing CD34 and Sca-1− , CD45− , Pecam− cells were purified by magnetic beads from the lungs of C57BL/6 mice 10 days following intratracheal administration of AdNull and AdShhN (5 × 1010 pu) or unilateral pneumonectomy and sham surgery.

    Isolation:

    Article Title: New Features on the Expression and Trafficking of mGluR1 Splice Variants Exposed by Two Novel Mutant Mouse Lines
    Article Snippet: Immunoblot Analysis and Co-immunoprecipitation Mouse cerebella were isolated and homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1% Triton X-100, protease inhibitor mixture (Complete EDTA-free, Roche) and phosphatase inhibitor cocktail mixture (PhosStop, Roche). .. Antigen-antibody complexes were detected with secondary antibodies conjugated with HRP (Jackson Immunoresearch, cat. no. 123-065-021, RRID:AB_2314646 ) and visualized by enhanced chemiluminescence (Amersham Hyperfilm ECL, GE Healthcare).

    Microscopy:

    Article Title: The increase of microRNA-21 during lung fibrosis and its contribution to epithelial-mesenchymal transition in pulmonary epithelial cells
    Article Snippet: Slides were then washed and incubated with biotin-SP-conjugated AffiniPure goat anti-horseradish peroxidase (1:200) at 37°C for 30 min (Jackson ImmunoResearch). .. After washing in water and counter-staining with Nuclear Fast Red, the slides were dehydrated and mounted for observation by microscopy.

    Article Title: Overexpression of Sonic Hedgehog in the Lung Mimics the Effect of Lung Injury and Compensatory Lung Growth on Pulmonary Sca-1 and CD34 Positive Cells
    Article Snippet: Sections were then counterstained with 4′,6-diamidino-2-phenylindole and visualized using an Olympus 1×70 microscope and Photometrics Quantix camera, and images were taken using MetaMorph software version 4.6. .. Formalin-fixed, paraffin-embedded slices were stained with rabbit anti-Ki67 antigen (Vector Labs, Burlingame, CA) followed by peroxidase-conjugated goat anti-rabbit (Jackson ImmunoResearch), biotin-conjugated goat anti–horseradish peroxidase (Jackson ImmunoResearch), and fluorescein isothiocyanate–conjugated streptavidin (Jackson ImmunoResearch).

    Mouse Assay:

    Article Title: Overexpression of Sonic Hedgehog in the Lung Mimics the Effect of Lung Injury and Compensatory Lung Growth on Pulmonary Sca-1 and CD34 Positive Cells
    Article Snippet: Formalin-fixed, paraffin-embedded slices were stained with rabbit anti-Ki67 antigen (Vector Labs, Burlingame, CA) followed by peroxidase-conjugated goat anti-rabbit (Jackson ImmunoResearch), biotin-conjugated goat anti–horseradish peroxidase (Jackson ImmunoResearch), and fluorescein isothiocyanate–conjugated streptavidin (Jackson ImmunoResearch). .. Sca-1+ , CD45− , Pecam− coexpressing CD34 and Sca-1− , CD45− , Pecam− cells were purified by magnetic beads from the lungs of C57BL/6 mice 10 days following intratracheal administration of AdNull and AdShhN (5 × 1010 pu) or unilateral pneumonectomy and sham surgery.

    Labeling:

    Article Title: The increase of microRNA-21 during lung fibrosis and its contribution to epithelial-mesenchymal transition in pulmonary epithelial cells
    Article Snippet: Slides were then washed and incubated with biotin-SP-conjugated AffiniPure goat anti-horseradish peroxidase (1:200) at 37°C for 30 min (Jackson ImmunoResearch). .. Finally, the slides were washed and labeled with Streptavidin-AP (Roche).

    Lysis:

    Article Title: New Features on the Expression and Trafficking of mGluR1 Splice Variants Exposed by Two Novel Mutant Mouse Lines
    Article Snippet: Immunoblot Analysis and Co-immunoprecipitation Mouse cerebella were isolated and homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1% Triton X-100, protease inhibitor mixture (Complete EDTA-free, Roche) and phosphatase inhibitor cocktail mixture (PhosStop, Roche). .. Antigen-antibody complexes were detected with secondary antibodies conjugated with HRP (Jackson Immunoresearch, cat. no. 123-065-021, RRID:AB_2314646 ) and visualized by enhanced chemiluminescence (Amersham Hyperfilm ECL, GE Healthcare).

    IA:

    Article Title: LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3
    Article Snippet: Mouse monoclonal antibody to Cxd2 (AM392; BioGenex, San Ramon, CA, USA) and Isl1 antibody (40.2D6; Developmental Studies Hybridoma Bank, Iowa City, IA,USA) were incubated on sections overnight at 4°C. .. Slides were then washed and incubated for horseradish peroxidase-conjugated streptavidin (123-065-021; Jackson ImmunoResearch) for 2 hours at room temperature.

    Purification:

    Article Title: Overexpression of Sonic Hedgehog in the Lung Mimics the Effect of Lung Injury and Compensatory Lung Growth on Pulmonary Sca-1 and CD34 Positive Cells
    Article Snippet: Formalin-fixed, paraffin-embedded slices were stained with rabbit anti-Ki67 antigen (Vector Labs, Burlingame, CA) followed by peroxidase-conjugated goat anti-rabbit (Jackson ImmunoResearch), biotin-conjugated goat anti–horseradish peroxidase (Jackson ImmunoResearch), and fluorescein isothiocyanate–conjugated streptavidin (Jackson ImmunoResearch). .. Sca-1+ , CD45− , Pecam− coexpressing CD34 and Sca-1− , CD45− , Pecam− cells were purified by magnetic beads from the lungs of C57BL/6 mice 10 days following intratracheal administration of AdNull and AdShhN (5 × 1010 pu) or unilateral pneumonectomy and sham surgery.

    In Situ Hybridization:

    Article Title: The increase of microRNA-21 during lung fibrosis and its contribution to epithelial-mesenchymal transition in pulmonary epithelial cells
    Article Snippet: Paragraph title: In situ hybridization (ISH) ... Slides were then washed and incubated with biotin-SP-conjugated AffiniPure goat anti-horseradish peroxidase (1:200) at 37°C for 30 min (Jackson ImmunoResearch).

    Software:

    Article Title: Overexpression of Sonic Hedgehog in the Lung Mimics the Effect of Lung Injury and Compensatory Lung Growth on Pulmonary Sca-1 and CD34 Positive Cells
    Article Snippet: Sections were then counterstained with 4′,6-diamidino-2-phenylindole and visualized using an Olympus 1×70 microscope and Photometrics Quantix camera, and images were taken using MetaMorph software version 4.6. .. Formalin-fixed, paraffin-embedded slices were stained with rabbit anti-Ki67 antigen (Vector Labs, Burlingame, CA) followed by peroxidase-conjugated goat anti-rabbit (Jackson ImmunoResearch), biotin-conjugated goat anti–horseradish peroxidase (Jackson ImmunoResearch), and fluorescein isothiocyanate–conjugated streptavidin (Jackson ImmunoResearch).

    Negative Control:

    Article Title: LIM homeodomain transcription factor Isl1 affects urethral epithelium differentiation and apoptosis via Shh
    Article Snippet: IgG was used as the negative control. .. A biotinylated goat anti-mouse secondary antibody (1:200; 115-065-146; Jackson ImmunoResearch, West Grove, PA, USA) was applied for 2 h at room temperature followed by streptavidin peroxidase (1:200; 123-065-021; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h. Diaminobenzidine (D4293; Sigma, St. Louis, MO, USA) and 0.1% H2 O2 were used for detecting peroxidase activity.

    Article Title: The increase of microRNA-21 during lung fibrosis and its contribution to epithelial-mesenchymal transition in pulmonary epithelial cells
    Article Snippet: The hybridization mixture contained 5 nM double-DIG LNA™ microRNA probe for miR-21 or scramble-miR as the negative control (Exiqon) and was applied and hybridized for 1 h at 53°C. .. Slides were then washed and incubated with biotin-SP-conjugated AffiniPure goat anti-horseradish peroxidase (1:200) at 37°C for 30 min (Jackson ImmunoResearch).

    Staining:

    Article Title: LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3
    Article Snippet: Slides were then washed and incubated for horseradish peroxidase-conjugated streptavidin (123-065-021; Jackson ImmunoResearch) for 2 hours at room temperature. .. Sections were photographed as described above (see Hematoxylin and eosin staining).

    Article Title: Overexpression of Sonic Hedgehog in the Lung Mimics the Effect of Lung Injury and Compensatory Lung Growth on Pulmonary Sca-1 and CD34 Positive Cells
    Article Snippet: .. Formalin-fixed, paraffin-embedded slices were stained with rabbit anti-Ki67 antigen (Vector Labs, Burlingame, CA) followed by peroxidase-conjugated goat anti-rabbit (Jackson ImmunoResearch), biotin-conjugated goat anti–horseradish peroxidase (Jackson ImmunoResearch), and fluorescein isothiocyanate–conjugated streptavidin (Jackson ImmunoResearch). ..

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  • Bioz Stars
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  • 99
    Jackson Immuno biotin sp affinipure goat anti horseradish peroxidase
    Biotin Sp Affinipure Goat Anti Horseradish Peroxidase, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti horseradish peroxidase/product/Jackson Immuno
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti horseradish peroxidase - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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    https://www.bioz.com/result/biotin sp affinipure goat anti rabbit igg/product/Jackson Immuno
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    biotin sp affinipure goat anti rabbit igg - by Bioz Stars, 2020-03
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