biotin sp affinipure goat anti chicken igy igg  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Goat Anti Chicken IgY IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule chicken IgY It also reacts with the light chains of other chicken immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine goat guinea pig syrian hamster horse human mouse rabbit rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    103-065-155
    Price:
    115
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    0 5
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure goat anti chicken igy igg
    Biotin SP AffiniPure Goat Anti Chicken IgY IgG
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule chicken IgY It also reacts with the light chains of other chicken immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine goat guinea pig syrian hamster horse human mouse rabbit rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure goat anti chicken igy igg/product/Jackson Immuno
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti chicken igy igg - by Bioz Stars, 2020-04
    98/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: Paragraph title: Qualitative IHC ... This was followed by a biotin-conjugated goat-anti-chicken secondary antibody (103-065-155) from Jackson ImmunoResearch Laboratories (West Grove, PA) diluted in 2% BlockHen II at 1:125 and incubated for 1 hr at 37°C.

    Over Expression:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: This was followed by a biotin-conjugated goat-anti-chicken secondary antibody (103-065-155) from Jackson ImmunoResearch Laboratories (West Grove, PA) diluted in 2% BlockHen II at 1:125 and incubated for 1 hr at 37°C. .. To confirm overexpression of native or recombinant KC protein, sections were incubated in rabbit anti-KC (5221-100, BioVision Research Products, Mountain View, CA) at 1:400 overnight at 4°C followed by biotin-conjugated goat-anti-rabbit (D-20695, Molecular Probes, Eugene, OR) at 1:500 for 45 min at 37°C.

    Avidin-Biotin Assay:

    Article Title: UBIAD1 Mutation Alters a Mitochondrial Prenyltransferase to Cause Schnyder Corneal Dystrophy
    Article Snippet: Cells were then blocked with 10% FBS in DPBS (FBS blocking solution) for 30 minutes, and then treated 15 minutes with avidin/biotin blocker (Vector Laboratories, Burlingame, CA) with a DPBS rinse between each step of the procedure described by the manufacturer. .. After three five-minute rinses with DPBS, biotinylated goat anti-chicken IgY (catalogue no. 103-065-155 from Jackson Immunoresearch, West Grove, PA) diluted to 5 µg/ml in FBS blocking solution was incubated with keratocytes for one hour.

    Labeling:

    Article Title: UBIAD1 Mutation Alters a Mitochondrial Prenyltransferase to Cause Schnyder Corneal Dystrophy
    Article Snippet: For immunofluorescence labeling experiments, the keratocytes were rinsed three times with DPBS before fixing with 2% formaldehyde for 10 minutes at room temperature. .. After three five-minute rinses with DPBS, biotinylated goat anti-chicken IgY (catalogue no. 103-065-155 from Jackson Immunoresearch, West Grove, PA) diluted to 5 µg/ml in FBS blocking solution was incubated with keratocytes for one hour.

    Transgenic Assay:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: This was followed by a biotin-conjugated goat-anti-chicken secondary antibody (103-065-155) from Jackson ImmunoResearch Laboratories (West Grove, PA) diluted in 2% BlockHen II at 1:125 and incubated for 1 hr at 37°C. .. To assess transgenic pro-collagen I stromal immunoreactivity, IHC was performed on representative samples, treated with 0.15% trypsin (1:9 dilution, ab970, Abcam) for 20 min at 37°C, using goat anti-mouse pro-collagen I (sc-8787, Santa Cruz Biotech, Santa Cruz, CA) at 1:350 overnight at 4°C, followed by biotin-conjugated donkey-anti-goat (sc-2042, Santa Cruz Biotech) at 1:400 for 45 min at 37°C.

    Incubation:

    Article Title: UBIAD1 Mutation Alters a Mitochondrial Prenyltransferase to Cause Schnyder Corneal Dystrophy
    Article Snippet: .. After three five-minute rinses with DPBS, biotinylated goat anti-chicken IgY (catalogue no. 103-065-155 from Jackson Immunoresearch, West Grove, PA) diluted to 5 µg/ml in FBS blocking solution was incubated with keratocytes for one hour. .. This primary labeling of UBIAD1 protein was then visualized by incubating keratocytes with 5 µg/ml Alexa 594 (red) streptavidin diluted in DPBS (catalog no. S32356, Molecular Probes, Eugene, Oregon).

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: .. This was followed by a biotin-conjugated goat-anti-chicken secondary antibody (103-065-155) from Jackson ImmunoResearch Laboratories (West Grove, PA) diluted in 2% BlockHen II at 1:125 and incubated for 1 hr at 37°C. .. To confirm overexpression of native or recombinant KC protein, sections were incubated in rabbit anti-KC (5221-100, BioVision Research Products, Mountain View, CA) at 1:400 overnight at 4°C followed by biotin-conjugated goat-anti-rabbit (D-20695, Molecular Probes, Eugene, OR) at 1:500 for 45 min at 37°C.

    Cell Culture:

    Article Title: UBIAD1 Mutation Alters a Mitochondrial Prenyltransferase to Cause Schnyder Corneal Dystrophy
    Article Snippet: Schnyder corneal dystrophy and normal human keratocytes were cultured at 37°C in Fibroblast Medium (catalogue no. 2301) also obtained from ScienCell Research Lab. .. After three five-minute rinses with DPBS, biotinylated goat anti-chicken IgY (catalogue no. 103-065-155 from Jackson Immunoresearch, West Grove, PA) diluted to 5 µg/ml in FBS blocking solution was incubated with keratocytes for one hour.

    Expressing:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: To verify protein expression by the transgene in ventral prostate epithelia via the V5 epitope tag, sections were blocked with BlockHen II (BH-1001, Aves Labs, Tigard, OR) for 30 min at 37°C, followed by chicken anti-V5 (NB600-379, Novus Biologicals, Littleton, CO) diluted in 2% BlockHen II at 1:300 and incubated overnight at 4°C. .. This was followed by a biotin-conjugated goat-anti-chicken secondary antibody (103-065-155) from Jackson ImmunoResearch Laboratories (West Grove, PA) diluted in 2% BlockHen II at 1:125 and incubated for 1 hr at 37°C.

    Marker:

    Article Title: UBIAD1 Mutation Alters a Mitochondrial Prenyltransferase to Cause Schnyder Corneal Dystrophy
    Article Snippet: After three five-minute rinses with DPBS, biotinylated goat anti-chicken IgY (catalogue no. 103-065-155 from Jackson Immunoresearch, West Grove, PA) diluted to 5 µg/ml in FBS blocking solution was incubated with keratocytes for one hour. .. To determine the subcellular localization of UBIAD1, keratocytes were further incubated one hour with either 5 µg/ml mouse IgG2b monoclonal anti-protein disulfide isomerase (catalogue no. S34253, Molecular Probes), an endoplasmic reticulum marker; or 5 µg/ml mouse IgG1 monoclonal anti-OXPHOS Complex I subunit, NADH dehydrogenase (catalogue no. A31857, Molecular Probes), a mitochondrial marker.

    Staining:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: All IHC staining utilized previously published protocols and reagents following secondary antibody [ , ]. .. This was followed by a biotin-conjugated goat-anti-chicken secondary antibody (103-065-155) from Jackson ImmunoResearch Laboratories (West Grove, PA) diluted in 2% BlockHen II at 1:125 and incubated for 1 hr at 37°C.

    Recombinant:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: This was followed by a biotin-conjugated goat-anti-chicken secondary antibody (103-065-155) from Jackson ImmunoResearch Laboratories (West Grove, PA) diluted in 2% BlockHen II at 1:125 and incubated for 1 hr at 37°C. .. To confirm overexpression of native or recombinant KC protein, sections were incubated in rabbit anti-KC (5221-100, BioVision Research Products, Mountain View, CA) at 1:400 overnight at 4°C followed by biotin-conjugated goat-anti-rabbit (D-20695, Molecular Probes, Eugene, OR) at 1:500 for 45 min at 37°C.

    Immunofluorescence:

    Article Title: UBIAD1 Mutation Alters a Mitochondrial Prenyltransferase to Cause Schnyder Corneal Dystrophy
    Article Snippet: For immunofluorescence labeling experiments, the keratocytes were rinsed three times with DPBS before fixing with 2% formaldehyde for 10 minutes at room temperature. .. After three five-minute rinses with DPBS, biotinylated goat anti-chicken IgY (catalogue no. 103-065-155 from Jackson Immunoresearch, West Grove, PA) diluted to 5 µg/ml in FBS blocking solution was incubated with keratocytes for one hour.

    Blocking Assay:

    Article Title: UBIAD1 Mutation Alters a Mitochondrial Prenyltransferase to Cause Schnyder Corneal Dystrophy
    Article Snippet: .. After three five-minute rinses with DPBS, biotinylated goat anti-chicken IgY (catalogue no. 103-065-155 from Jackson Immunoresearch, West Grove, PA) diluted to 5 µg/ml in FBS blocking solution was incubated with keratocytes for one hour. .. This primary labeling of UBIAD1 protein was then visualized by incubating keratocytes with 5 µg/ml Alexa 594 (red) streptavidin diluted in DPBS (catalog no. S32356, Molecular Probes, Eugene, Oregon).

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  • 98
    Jackson Immuno biotin sp affinipure goat anti chicken igy igg
    Biotin Sp Affinipure Goat Anti Chicken Igy Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti chicken igy igg/product/Jackson Immuno
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti chicken igy igg - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

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    https://www.bioz.com/result/biotin sp affinipure f/product/Jackson Immuno
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f - by Bioz Stars, 2020-04
    99/100 stars
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