biotin sp affinipure goat anti armenian hamster igg  (Jackson Immuno)

 
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  • 99
    Name:
    Biotin SP AffiniPure Goat Anti Armenian Hamster IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule armenian hamster IgG It also reacts with the light chains of other armenian hamster immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    127-065-099
    Price:
    121
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    1 5
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure goat anti armenian hamster igg
    Biotin SP AffiniPure Goat Anti Armenian Hamster IgG
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule armenian hamster IgG It also reacts with the light chains of other armenian hamster immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure goat anti armenian hamster igg/product/Jackson Immuno
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti armenian hamster igg - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: Paragraph title: Immunohistochemistry ... Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen).

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: Paragraph title: Histology and immunohistochemistry in mice ... Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014).

    Amplification:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: Fluorescent immunohistochemistry of 4% paraformaldehyde-fixed cryosections was performed using the tyramide signal amplification (TSA) Fluorescence/ Cyanine 3 system (Fluorescein NEL701 A, Cyanine NEL 704 A, PerkinElmer) and a fluorescence microscope (IX70; Olympus or Leica SP8, Leica) using primary antibodies against F4/80 (1:100 dilution, AP10243P4-5, Acris), IgM biotin (1:100 dilution, 98673, Abcam), FoxP3 (1:100 dilution, 14-5773-82, eBioscience), CD3 (1:100 dilution, 16-0031-82, eBioscience), B220 (1:100 dilution, 553092, BD Bioscience), CCL-20-Allophycocyanin conjugated (1:50 dilution, IC760A, R + D Systems) and γδTCR 1:100 dilution, 13-58811-81, eBioscience) respectively. .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen).

    Fluorescence:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: Nuclei were counterstained with Hoechst 3342 (Invitrogen) or mounting medium for fluorescence with 4,6-diamidino-2-phenylindole (H-1200, Vector). .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen).

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: Immunofluorescence of cryosections was performed using the TSA Cy3 System (NEL704A001KT, PerkinElmer) and a fluorescence microscope (IX70; Olympus) using primary antibodies against CD4 (catalogue number: 553043, BD Pharmingen, rm4-5, 1:00 dilution), CD11c (catalogue number 550283, BD, dilution 1:200), MPO (ab15484 Abcam, 1: 20 dilution), F4/80 (BM8 eBioscience, lot 14-4801-81, 1: 1,000 dilution) and TCRγδ (BD Bioscience, clon No: h57-597 catalogue number: 553169, dilution 1:100). .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014).

    Avidin-Biotin Assay:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: In brief, cryosections were fixed in 4% PFA for 20 min followed by sequential incubation with methanol, avidin/biotin (Vector Laboratories) and protein blocking reagent (T144.1 Roti-ImmunoBlock, Roth) to eliminate unspecific background staining. .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen).

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: In brief, cryosections were fixed in 4% PFA for 20 min followed by sequential incubation with methanol, avidin/biotin (Vector Laboratories) and protein blocking reagent (catalogue number: T144.1 Roti-ImmunoBlock, Roth) to eliminate unspecific background staining. .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014).

    Blocking Assay:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: In brief, cryosections were fixed in 4% PFA for 20 min followed by sequential incubation with methanol, avidin/biotin (Vector Laboratories) and protein blocking reagent (T144.1 Roti-ImmunoBlock, Roth) to eliminate unspecific background staining. .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen).

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: In brief, cryosections were fixed in 4% PFA for 20 min followed by sequential incubation with methanol, avidin/biotin (Vector Laboratories) and protein blocking reagent (catalogue number: T144.1 Roti-ImmunoBlock, Roth) to eliminate unspecific background staining. .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014).

    Mouse Assay:

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: Paragraph title: Histology and immunohistochemistry in mice ... Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014).

    Incubation:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen). .. All samples were finally treated with streptavidin-horseradish peroxidase and stained with TSA Fluorescence/Cyanine 3 systems according to the manufacturer’s instructions (Fluorescein NEL701 A, Cyanine NEL 704 A, PerkinElmer).

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014). .. All samples were finally treated with streptavidin-horseradish peroxidase and stained with Tyramide (Cy3) according to the manufacturer's instructions (catalogue number: NEL704A001KT, PerkinElmer).

    Microscopy:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: Fluorescent immunohistochemistry of 4% paraformaldehyde-fixed cryosections was performed using the tyramide signal amplification (TSA) Fluorescence/ Cyanine 3 system (Fluorescein NEL701 A, Cyanine NEL 704 A, PerkinElmer) and a fluorescence microscope (IX70; Olympus or Leica SP8, Leica) using primary antibodies against F4/80 (1:100 dilution, AP10243P4-5, Acris), IgM biotin (1:100 dilution, 98673, Abcam), FoxP3 (1:100 dilution, 14-5773-82, eBioscience), CD3 (1:100 dilution, 16-0031-82, eBioscience), B220 (1:100 dilution, 553092, BD Bioscience), CCL-20-Allophycocyanin conjugated (1:50 dilution, IC760A, R + D Systems) and γδTCR 1:100 dilution, 13-58811-81, eBioscience) respectively. .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen).

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: Immunofluorescence of cryosections was performed using the TSA Cy3 System (NEL704A001KT, PerkinElmer) and a fluorescence microscope (IX70; Olympus) using primary antibodies against CD4 (catalogue number: 553043, BD Pharmingen, rm4-5, 1:00 dilution), CD11c (catalogue number 550283, BD, dilution 1:200), MPO (ab15484 Abcam, 1: 20 dilution), F4/80 (BM8 eBioscience, lot 14-4801-81, 1: 1,000 dilution) and TCRγδ (BD Bioscience, clon No: h57-597 catalogue number: 553169, dilution 1:100). .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014).

    Staining:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: In brief, cryosections were fixed in 4% PFA for 20 min followed by sequential incubation with methanol, avidin/biotin (Vector Laboratories) and protein blocking reagent (T144.1 Roti-ImmunoBlock, Roth) to eliminate unspecific background staining. .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen).

    Article Title: Protective Role of ?? T cells in Spontaneous Ocular Inflammation
    Article Snippet: .. Corneas were frozen in OCT compound, as previously described for lung tissue , and acetone-dehydrated 10 µm sections stained with a pan-reactive anti-TCRβ monoclonal antibody (H57-597 ) plus an anti-hamster immunoglobulin biotin-conjugated antibody (Jackson ImmunoResearch, cat. no. 127-065-160) and Cy3-conjugated streptavidin (Jackson ImmunoResearch, cat. no. 016-160-084), together with either anti-CD4 (clone GK1.5 ) or anti-CD8α (clone 53.6.7 ) monoclonal antibodies plus an anti-rat immunoglobulin Cy5-conjugated F(ab’)2 antibody (Jackson ImmunoResearch, cat. no. 712-176-153). .. Two-color flow cytometry was carried on nylon-wool purified spleen or peripheral blood cells as previously described .

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: In brief, cryosections were fixed in 4% PFA for 20 min followed by sequential incubation with methanol, avidin/biotin (Vector Laboratories) and protein blocking reagent (catalogue number: T144.1 Roti-ImmunoBlock, Roth) to eliminate unspecific background staining. .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014).

    Immunofluorescence:

    Article Title: Protective Role of ?? T cells in Spontaneous Ocular Inflammation
    Article Snippet: Paragraph title: Immunofluorescence ... Corneas were frozen in OCT compound, as previously described for lung tissue , and acetone-dehydrated 10 µm sections stained with a pan-reactive anti-TCRβ monoclonal antibody (H57-597 ) plus an anti-hamster immunoglobulin biotin-conjugated antibody (Jackson ImmunoResearch, cat. no. 127-065-160) and Cy3-conjugated streptavidin (Jackson ImmunoResearch, cat. no. 016-160-084), together with either anti-CD4 (clone GK1.5 ) or anti-CD8α (clone 53.6.7 ) monoclonal antibodies plus an anti-rat immunoglobulin Cy5-conjugated F(ab’)2 antibody (Jackson ImmunoResearch, cat. no. 712-176-153).

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: Immunofluorescence of cryosections was performed using the TSA Cy3 System (NEL704A001KT, PerkinElmer) and a fluorescence microscope (IX70; Olympus) using primary antibodies against CD4 (catalogue number: 553043, BD Pharmingen, rm4-5, 1:00 dilution), CD11c (catalogue number 550283, BD, dilution 1:200), MPO (ab15484 Abcam, 1: 20 dilution), F4/80 (BM8 eBioscience, lot 14-4801-81, 1: 1,000 dilution) and TCRγδ (BD Bioscience, clon No: h57-597 catalogue number: 553169, dilution 1:100). .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014).

    Plasmid Preparation:

    Article Title: Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment
    Article Snippet: Nuclei were counterstained with Hoechst 3342 (Invitrogen) or mounting medium for fluorescence with 4,6-diamidino-2-phenylindole (H-1200, Vector). .. Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (1:300 dilution, 127-065-160 Jackson Immunoresearch and 554014, BD Pharmingen).

    Article Title: Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis
    Article Snippet: Subsequently, the slides were incubated for 30 min at room temperature with biotinylated secondary antibodies (Jackson Immunoresearch catalogue number: 127-065-160 and BD Pharmingen catalogue number: 554014). .. Before examination, nuclei were counterstained with mounting medium for fluorescence with 4,6-diamidino-2-phenylindole (catalogue number: H-1200, Vector).

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  • About
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  • Press Release
  • Team
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  • Contact
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  • 99
    Jackson Immuno biotin sp affinipure goat anti armenian hamster igg
    Biotin Sp Affinipure Goat Anti Armenian Hamster Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti armenian hamster igg/product/Jackson Immuno
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti armenian hamster igg - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Jackson Immuno biotin sp
    Biotin Sp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp/product/Jackson Immuno
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp - by Bioz Stars, 2020-04
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