biotin sp affinipure fab fragment goat anti rat igg  (Jackson Immuno)

 
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  • 99
    Name:
    Biotin SP AffiniPure Fab Fragment Goat Anti Rat IgG
    Description:
    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion including the hinge region These antibodies are monovalent containing only a single antigen binding site The molecular weight of Fab fragments is about 50 kDa Based on antigen binding assay and or ELISA the antibody reacts with whole molecule rat IgG It also reacts with the light chains of other rat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    112-067-003
    Price:
    97
    Purity:
    The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    0 5
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure fab fragment goat anti rat igg
    Biotin SP AffiniPure Fab Fragment Goat Anti Rat IgG
    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion including the hinge region These antibodies are monovalent containing only a single antigen binding site The molecular weight of Fab fragments is about 50 kDa Based on antigen binding assay and or ELISA the antibody reacts with whole molecule rat IgG It also reacts with the light chains of other rat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure fab fragment goat anti rat igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure fab fragment goat anti rat igg - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: T-cell-dependent mechanisms promote Ebola VLP-induced antibody responses, but are dispensable for vaccine-mediated protection
    Article Snippet: Paragraph title: Flow cytometry ... T follicular helper cell staining was performed by a primary incubation with CXCR5 (BD Clone 2G8) followed by secondary incubation using goat anti-rat (H+L)-biotin (Jackson ImmunoResearch, West Grove, PA, USA 112-067-003).

    Article Title: CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development
    Article Snippet: .. Antibodies, iNKT tetramer, and flow cytometry Flow cytometry reagents used were: TCRb (H57-597, eBioscience), CD4 (RM4-5, eBioscience), CD8a (53–6.7, eBioscience), CD21 (8D9, eBioscience), CD23 (B3B4, eBioscience), CD44 (IM7, eBioscience), NK1.1 (PK136, eBioscience), CD1d tetramer (PBS57, NIH Tetramer Core Facility), 2B4 (2B4, BD Biosciences), Ly9 (Ly9ab3, Biolegend), B220 (RA3-6B2, eBioscience), CD19 (1D3, eBioscience), Fas (15A7, eBioscience), GL-7 (GL-7, eBioscience), PD-1 (RMP-130, Biolegend), CXCR5 (2G8, BD Biosciences), biotin goat anti-rat (cat# 112-067-003, Jackson Immunoresearch), fluorophore-conjugated SAv (eBioscience). .. CXCR5 staining was performed as previously described [ ].

    Isolation:

    Article Title: Adjuvant-enhanced CD4 T Cell Responses are Critical to Durable Vaccine Immunity
    Article Snippet: Paragraph title: Popliteal Lymph Isolation and Identification of T Follicular Helper Cell Populations ... CXCR5 was identified by incubating cells with purified rat anti-mouse CXCR5 (BD Clone 2G8) at 1:100 in buffer A (PBS + 0.5% BSA + 0.1% sodium azide supplemented with 2% normal mouse serum (NMS) and 2% FCS) for 1 h. Cells were then washed two times and incubated with goat anti-rat (H + L)-biotin (Jackson ImmunoResearch, 112-067-003) in buffer A for 30 min.

    Cytometry:

    Article Title: T-cell-dependent mechanisms promote Ebola VLP-induced antibody responses, but are dispensable for vaccine-mediated protection
    Article Snippet: Paragraph title: Flow cytometry ... T follicular helper cell staining was performed by a primary incubation with CXCR5 (BD Clone 2G8) followed by secondary incubation using goat anti-rat (H+L)-biotin (Jackson ImmunoResearch, West Grove, PA, USA 112-067-003).

    Article Title: CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development
    Article Snippet: .. Antibodies, iNKT tetramer, and flow cytometry Flow cytometry reagents used were: TCRb (H57-597, eBioscience), CD4 (RM4-5, eBioscience), CD8a (53–6.7, eBioscience), CD21 (8D9, eBioscience), CD23 (B3B4, eBioscience), CD44 (IM7, eBioscience), NK1.1 (PK136, eBioscience), CD1d tetramer (PBS57, NIH Tetramer Core Facility), 2B4 (2B4, BD Biosciences), Ly9 (Ly9ab3, Biolegend), B220 (RA3-6B2, eBioscience), CD19 (1D3, eBioscience), Fas (15A7, eBioscience), GL-7 (GL-7, eBioscience), PD-1 (RMP-130, Biolegend), CXCR5 (2G8, BD Biosciences), biotin goat anti-rat (cat# 112-067-003, Jackson Immunoresearch), fluorophore-conjugated SAv (eBioscience). .. CXCR5 staining was performed as previously described [ ].

    Purification:

    Article Title: Adjuvant-enhanced CD4 T Cell Responses are Critical to Durable Vaccine Immunity
    Article Snippet: .. CXCR5 was identified by incubating cells with purified rat anti-mouse CXCR5 (BD Clone 2G8) at 1:100 in buffer A (PBS + 0.5% BSA + 0.1% sodium azide supplemented with 2% normal mouse serum (NMS) and 2% FCS) for 1 h. Cells were then washed two times and incubated with goat anti-rat (H + L)-biotin (Jackson ImmunoResearch, 112-067-003) in buffer A for 30 min. .. Cells were then washed twice and incubated with streptavidin-PECy7 (BD 557598), anti-CD3-V450 (BD Clone 500A2), anti-CD4-PerCyCy5.5 (BD Clone RM4-5), anti-PD1-APC (eBioscience Clone RMP1-30), and anti-ICOS-PE (BD Clone 7E.17G9) in buffer A for a further 30 min. After two more washes, cells were incubated with BD Phosflow Lyse/Fix buffer (BD 558049) for 12 min at 37 °C, washed two times, and incubated with anti-Bcl6-Alexa488 (BD Clone K112-91) for 1 h in Perm/Wash Buffer I (BD 557885).

    Activation Assay:

    Article Title: T-cell-dependent mechanisms promote Ebola VLP-induced antibody responses, but are dispensable for vaccine-mediated protection
    Article Snippet: The B-cell staining included B220 (Becton-Dickinson Biosciences (BD), Franklin Lanes, NJ, USA Clone RA3-6B2), IgM (BD Clone R6-60.2), IgD (eBioscience, San Diego, CA, USA Clone 11-26C), CD38 (BD Clone 90), CD95 (BD Clone Jo-2), and T & B Cell Activation Antigen (BD Clone GL-7). .. T follicular helper cell staining was performed by a primary incubation with CXCR5 (BD Clone 2G8) followed by secondary incubation using goat anti-rat (H+L)-biotin (Jackson ImmunoResearch, West Grove, PA, USA 112-067-003).

    Incubation:

    Article Title: T-cell-dependent mechanisms promote Ebola VLP-induced antibody responses, but are dispensable for vaccine-mediated protection
    Article Snippet: .. T follicular helper cell staining was performed by a primary incubation with CXCR5 (BD Clone 2G8) followed by secondary incubation using goat anti-rat (H+L)-biotin (Jackson ImmunoResearch, West Grove, PA, USA 112-067-003). .. Subsequently, the cells were counter-stained with streptavidin (BD 557598), CD3 (BD Clone 500A2), CD4 (BD Clone RM4-5), PD-1 (eBioscience Clone RMP1-30), and ICOS (BD Clone 7E.17G9).

    Article Title: Adjuvant-enhanced CD4 T Cell Responses are Critical to Durable Vaccine Immunity
    Article Snippet: .. CXCR5 was identified by incubating cells with purified rat anti-mouse CXCR5 (BD Clone 2G8) at 1:100 in buffer A (PBS + 0.5% BSA + 0.1% sodium azide supplemented with 2% normal mouse serum (NMS) and 2% FCS) for 1 h. Cells were then washed two times and incubated with goat anti-rat (H + L)-biotin (Jackson ImmunoResearch, 112-067-003) in buffer A for 30 min. .. Cells were then washed twice and incubated with streptavidin-PECy7 (BD 557598), anti-CD3-V450 (BD Clone 500A2), anti-CD4-PerCyCy5.5 (BD Clone RM4-5), anti-PD1-APC (eBioscience Clone RMP1-30), and anti-ICOS-PE (BD Clone 7E.17G9) in buffer A for a further 30 min. After two more washes, cells were incubated with BD Phosflow Lyse/Fix buffer (BD 558049) for 12 min at 37 °C, washed two times, and incubated with anti-Bcl6-Alexa488 (BD Clone K112-91) for 1 h in Perm/Wash Buffer I (BD 557885).

    FACS:

    Article Title: T-cell-dependent mechanisms promote Ebola VLP-induced antibody responses, but are dispensable for vaccine-mediated protection
    Article Snippet: The cells were washed using FACS buffer (PBS, 0.5% BSA and 2 mM EDTA; Corning, Sigma, St Louis, MO, USA), lysed with red blood cell (RBC) buffer (Sigma), and subsequently counter-stained. .. T follicular helper cell staining was performed by a primary incubation with CXCR5 (BD Clone 2G8) followed by secondary incubation using goat anti-rat (H+L)-biotin (Jackson ImmunoResearch, West Grove, PA, USA 112-067-003).

    Article Title: Adjuvant-enhanced CD4 T Cell Responses are Critical to Durable Vaccine Immunity
    Article Snippet: CXCR5 was identified by incubating cells with purified rat anti-mouse CXCR5 (BD Clone 2G8) at 1:100 in buffer A (PBS + 0.5% BSA + 0.1% sodium azide supplemented with 2% normal mouse serum (NMS) and 2% FCS) for 1 h. Cells were then washed two times and incubated with goat anti-rat (H + L)-biotin (Jackson ImmunoResearch, 112-067-003) in buffer A for 30 min. .. Cells were washed two times and examined on the BD FACS Canto II.

    Staining:

    Article Title: T-cell-dependent mechanisms promote Ebola VLP-induced antibody responses, but are dispensable for vaccine-mediated protection
    Article Snippet: .. T follicular helper cell staining was performed by a primary incubation with CXCR5 (BD Clone 2G8) followed by secondary incubation using goat anti-rat (H+L)-biotin (Jackson ImmunoResearch, West Grove, PA, USA 112-067-003). .. Subsequently, the cells were counter-stained with streptavidin (BD 557598), CD3 (BD Clone 500A2), CD4 (BD Clone RM4-5), PD-1 (eBioscience Clone RMP1-30), and ICOS (BD Clone 7E.17G9).

    Article Title: CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development
    Article Snippet: Antibodies, iNKT tetramer, and flow cytometry Flow cytometry reagents used were: TCRb (H57-597, eBioscience), CD4 (RM4-5, eBioscience), CD8a (53–6.7, eBioscience), CD21 (8D9, eBioscience), CD23 (B3B4, eBioscience), CD44 (IM7, eBioscience), NK1.1 (PK136, eBioscience), CD1d tetramer (PBS57, NIH Tetramer Core Facility), 2B4 (2B4, BD Biosciences), Ly9 (Ly9ab3, Biolegend), B220 (RA3-6B2, eBioscience), CD19 (1D3, eBioscience), Fas (15A7, eBioscience), GL-7 (GL-7, eBioscience), PD-1 (RMP-130, Biolegend), CXCR5 (2G8, BD Biosciences), biotin goat anti-rat (cat# 112-067-003, Jackson Immunoresearch), fluorophore-conjugated SAv (eBioscience). .. CXCR5 staining was performed as previously described [ ].

    Article Title: Adjuvant-enhanced CD4 T Cell Responses are Critical to Durable Vaccine Immunity
    Article Snippet: T follicular helper (Tfh) cell populations were identified by surface staining with CD3, CD4, CXCR5, ICOS, and PD1 as well as intracellular staining for Bcl6. .. CXCR5 was identified by incubating cells with purified rat anti-mouse CXCR5 (BD Clone 2G8) at 1:100 in buffer A (PBS + 0.5% BSA + 0.1% sodium azide supplemented with 2% normal mouse serum (NMS) and 2% FCS) for 1 h. Cells were then washed two times and incubated with goat anti-rat (H + L)-biotin (Jackson ImmunoResearch, 112-067-003) in buffer A for 30 min.

    Mouse Assay:

    Article Title: Adjuvant-enhanced CD4 T Cell Responses are Critical to Durable Vaccine Immunity
    Article Snippet: 2.10 Popliteal Lymph Isolation and Identification of T Follicular Helper Cell Populations Popliteal LN were isolated from vaccinated mice 7 days after vaccination. .. CXCR5 was identified by incubating cells with purified rat anti-mouse CXCR5 (BD Clone 2G8) at 1:100 in buffer A (PBS + 0.5% BSA + 0.1% sodium azide supplemented with 2% normal mouse serum (NMS) and 2% FCS) for 1 h. Cells were then washed two times and incubated with goat anti-rat (H + L)-biotin (Jackson ImmunoResearch, 112-067-003) in buffer A for 30 min.

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  • News
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  • 96
    Jackson Immuno biotin sp affinipure fab fragment goat anti rat igg h l
    Biotin Sp Affinipure Fab Fragment Goat Anti Rat Igg H L, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure fab fragment goat anti rat igg h l/product/Jackson Immuno
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure fab fragment goat anti rat igg h l - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    99
    Jackson Immuno biotin sp affinipure fab fragment goat anti rat igg
    Biotin Sp Affinipure Fab Fragment Goat Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure fab fragment goat anti rat igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure fab fragment goat anti rat igg - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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