biotin sp affinipure fab fragment goat anti mouse igg  (Jackson Immuno)

 
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  • 99
    Name:
    Biotin SP AffiniPure Fab Fragment Goat Anti Mouse IgG
    Description:
    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion including the hinge region These antibodies are monovalent containing only a single antigen binding site The molecular weight of Fab fragments is about 50 kDa Based on antigen binding assay and or ELISA the antibody reacts with whole molecule mouse IgG It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    115-067-003
    Price:
    97
    Purity:
    The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    0 5
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure fab fragment goat anti mouse igg
    Biotin SP AffiniPure Fab Fragment Goat Anti Mouse IgG
    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion including the hinge region These antibodies are monovalent containing only a single antigen binding site The molecular weight of Fab fragments is about 50 kDa Based on antigen binding assay and or ELISA the antibody reacts with whole molecule mouse IgG It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure fab fragment goat anti mouse igg/product/Jackson Immuno
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure fab fragment goat anti mouse igg - by Bioz Stars, 2020-04
    99/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Effects of tumor grade and dexamethasone on myeloid cells in patients with glioma
    Article Snippet: Paragraph title: IHC ... A biotinylated anti-mouse secondary antibody (Jackson Immuno #115–067-003) was applied for 30 minutes.

    Article Title: Tracking Modified Vaccinia Virus Ankara in the Chicken Embryo: In Vivo Tropism and Pathogenesis of Egg Infections
    Article Snippet: Paragraph title: 2.4. Histology and Immunohistochemistry ... Biotinylated secondary antibodies were a goat-anti-mouse Fab-fragment (1:200, Jackson Immunoresearch, 115-067-003, Cambridge, UK) and a goat anti-rabbit antibody (1:200, Vector, BA-1000, Burlingame, CA, USA).

    Article Title: Liver kinase B1/AMP‐activated protein kinase‐mediated regulation by gentiopicroside ameliorates P2X7 receptor‐dependent alcoholic hepatosteatosis
    Article Snippet: Paragraph title: Immunohistochemistry ... The sections were then incubated with biotin‐SP affiniPure Fab fragment goat anti‐mouse IgG (Code Number: 115‐067‐003, Jackson ImmunoResearch Labs) as secondary antibodies.

    Amplification:

    Article Title: Tracking Modified Vaccinia Virus Ankara in the Chicken Embryo: In Vivo Tropism and Pathogenesis of Egg Infections
    Article Snippet: Biotinylated secondary antibodies were a goat-anti-mouse Fab-fragment (1:200, Jackson Immunoresearch, 115-067-003, Cambridge, UK) and a goat anti-rabbit antibody (1:200, Vector, BA-1000, Burlingame, CA, USA). .. MVA-Kul01-double-IHC was done sequentially, first MVA-IHC with Streptavidin Alexa Fluor 568 (1:100, ThermoFisher, S-11226, Waltham, MA, USA), followed by avidin-biotin-block and Kul01-IHC with ABC-HRP and tyramide signal amplification with Alexa Fluor 488 (ThermoFisher, B40953) and nuclear DAPI-staining.

    Avidin-Biotin Assay:

    Article Title: Effects of tumor grade and dexamethasone on myeloid cells in patients with glioma
    Article Snippet: A solution of 5% goat serum (Jackson Immuno #005–000-121) + 0.3% Triton X (“Tx”, Thermo Fisher #BP151100) in PBS + Avidin/Biotin solution (Vector #SP-2001) was applied to block nonspecific protein, endogenous biotin and avidin binding sites. .. A biotinylated anti-mouse secondary antibody (Jackson Immuno #115–067-003) was applied for 30 minutes.

    Article Title: Tracking Modified Vaccinia Virus Ankara in the Chicken Embryo: In Vivo Tropism and Pathogenesis of Egg Infections
    Article Snippet: IHC was performed with preceding avidin-biotin-block and without epitope retrieval [ ]. .. Biotinylated secondary antibodies were a goat-anti-mouse Fab-fragment (1:200, Jackson Immunoresearch, 115-067-003, Cambridge, UK) and a goat anti-rabbit antibody (1:200, Vector, BA-1000, Burlingame, CA, USA).

    Article Title: Liver kinase B1/AMP‐activated protein kinase‐mediated regulation by gentiopicroside ameliorates P2X7 receptor‐dependent alcoholic hepatosteatosis
    Article Snippet: Slides were treated with 3% hydrogen peroxide, followed by blocking with 5% normal goat serum and Avidin/Biotin Blocking solution (Vector Laboratories, Inc., Burlingame, CA, USA). .. The sections were then incubated with biotin‐SP affiniPure Fab fragment goat anti‐mouse IgG (Code Number: 115‐067‐003, Jackson ImmunoResearch Labs) as secondary antibodies.

    Blocking Assay:

    Article Title: Effects of tumor grade and dexamethasone on myeloid cells in patients with glioma
    Article Snippet: A solution of 5% goat serum (Jackson Immuno #005–000-121) + 0.3% Triton X (“Tx”, Thermo Fisher #BP151100) in PBS + Avidin/Biotin solution (Vector #SP-2001) was applied to block nonspecific protein, endogenous biotin and avidin binding sites. .. A biotinylated anti-mouse secondary antibody (Jackson Immuno #115–067-003) was applied for 30 minutes.

    Article Title: Liver kinase B1/AMP‐activated protein kinase‐mediated regulation by gentiopicroside ameliorates P2X7 receptor‐dependent alcoholic hepatosteatosis
    Article Snippet: Slides were treated with 3% hydrogen peroxide, followed by blocking with 5% normal goat serum and Avidin/Biotin Blocking solution (Vector Laboratories, Inc., Burlingame, CA, USA). .. The sections were then incubated with biotin‐SP affiniPure Fab fragment goat anti‐mouse IgG (Code Number: 115‐067‐003, Jackson ImmunoResearch Labs) as secondary antibodies.

    Light Microscopy:

    Article Title: Liver kinase B1/AMP‐activated protein kinase‐mediated regulation by gentiopicroside ameliorates P2X7 receptor‐dependent alcoholic hepatosteatosis
    Article Snippet: The sections were then incubated with biotin‐SP affiniPure Fab fragment goat anti‐mouse IgG (Code Number: 115‐067‐003, Jackson ImmunoResearch Labs) as secondary antibodies. .. The sections were then incubated with biotin‐SP affiniPure Fab fragment goat anti‐mouse IgG (Code Number: 115‐067‐003, Jackson ImmunoResearch Labs) as secondary antibodies.

    Incubation:

    Article Title: GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl−-sensitive WNK1 kinase
    Article Snippet: .. Briefly, cells were incubated for 3–5 min at 37 °C with primary antibodies against Flag (mouse, 1:700, Sigma, cat #F3165), washed, and incubated for 3–5 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 605 nm (1 nM; Invitrogen) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Article Title: MicroRNA-132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras-MAPK pathway
    Article Snippet: Then sections were incubated with primary antibodies diluted in 0.5% BSA in TBST overnight at 4°C. .. Anti-RASA1 antibody was diluted in 0.5% BSA in TBST, applied on the sections overnight at 4°C, followed by biotin-sp-conjugated affini-pure Fab fragment goat anti-mouse IgG(H+L) (1:500, 115-067-003; Jackson Immunoresearch Laboratory) and streptavidin-conjugated Alexa 555.

    Article Title: A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining
    Article Snippet: To create an antibody complex, we combined a biotinylated goat anti-mouse IgG Fab fragment (#115-067-003; Jackson ImmunoResearch Laboratories; West Grove, PA), the desired mouse primary antibody and antibody diluent in a tube at room temperature for 20 min. .. To ensure that non-complexed secondary antibody cannot bind to endogenous immunoglobulin in the mouse tissue, 10 µl of normal mouse serum (#015-000-120; Jackson ImmunoResearch) was added per 1 µg of secondary antibody and the tube incubated for an additional 10 min ( ).

    Article Title: Liver kinase B1/AMP‐activated protein kinase‐mediated regulation by gentiopicroside ameliorates P2X7 receptor‐dependent alcoholic hepatosteatosis
    Article Snippet: .. The sections were then incubated with biotin‐SP affiniPure Fab fragment goat anti‐mouse IgG (Code Number: 115‐067‐003, Jackson ImmunoResearch Labs) as secondary antibodies. .. Bound antibodies were visualized with Lab Vision™ DAB Plus Substrate Staining System (Thermo Fisher Scientific, Fremont, CA, USA).

    Imaging:

    Article Title: GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl−-sensitive WNK1 kinase
    Article Snippet: .. Briefly, cells were incubated for 3–5 min at 37 °C with primary antibodies against Flag (mouse, 1:700, Sigma, cat #F3165), washed, and incubated for 3–5 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium. .. After washes, cells were incubated for 1 min with streptavidin-coated quantum dots (QDs) emitting at 605 nm (1 nM; Invitrogen) in borate buffer (50 mM) supplemented with sucrose (200 mM) or in PBS (1 M; Invitrogen) supplemented with 10% Casein (v/v) (Sigma).

    Staining:

    Article Title: GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl−-sensitive WNK1 kinase
    Article Snippet: Paragraph title: Live cell staining for single-particle imaging ... Briefly, cells were incubated for 3–5 min at 37 °C with primary antibodies against Flag (mouse, 1:700, Sigma, cat #F3165), washed, and incubated for 3–5 min at 37 °C with biotinylated Fab secondary antibodies (goat anti-mouse: 1:700; Jackson Immuno research, cat #115-067-003, West Grove, USA) in imaging medium.

    Article Title: MicroRNA-132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras-MAPK pathway
    Article Snippet: Paragraph title: Immunofluorescent staining ... Anti-RASA1 antibody was diluted in 0.5% BSA in TBST, applied on the sections overnight at 4°C, followed by biotin-sp-conjugated affini-pure Fab fragment goat anti-mouse IgG(H+L) (1:500, 115-067-003; Jackson Immunoresearch Laboratory) and streptavidin-conjugated Alexa 555.

    Article Title: A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining
    Article Snippet: Paragraph title: Staining Method Using a Biotinylated Goat Secondary ... To create an antibody complex, we combined a biotinylated goat anti-mouse IgG Fab fragment (#115-067-003; Jackson ImmunoResearch Laboratories; West Grove, PA), the desired mouse primary antibody and antibody diluent in a tube at room temperature for 20 min.

    Article Title: Liver kinase B1/AMP‐activated protein kinase‐mediated regulation by gentiopicroside ameliorates P2X7 receptor‐dependent alcoholic hepatosteatosis
    Article Snippet: Immunohistochemical staining for SREBP1 was performed using a mouse anti‐SREBP1 monoclonal antibody (ab3259, Abcam, Cambridge, MA). .. The sections were then incubated with biotin‐SP affiniPure Fab fragment goat anti‐mouse IgG (Code Number: 115‐067‐003, Jackson ImmunoResearch Labs) as secondary antibodies.

    Immunostaining:

    Article Title: Effects of tumor grade and dexamethasone on myeloid cells in patients with glioma
    Article Snippet: IHC Immunostaining was performed manually. .. A biotinylated anti-mouse secondary antibody (Jackson Immuno #115–067-003) was applied for 30 minutes.

    Binding Assay:

    Article Title: Effects of tumor grade and dexamethasone on myeloid cells in patients with glioma
    Article Snippet: A solution of 5% goat serum (Jackson Immuno #005–000-121) + 0.3% Triton X (“Tx”, Thermo Fisher #BP151100) in PBS + Avidin/Biotin solution (Vector #SP-2001) was applied to block nonspecific protein, endogenous biotin and avidin binding sites. .. A biotinylated anti-mouse secondary antibody (Jackson Immuno #115–067-003) was applied for 30 minutes.

    Cross-linking Immunoprecipitation:

    Article Title: Tracking Modified Vaccinia Virus Ankara in the Chicken Embryo: In Vivo Tropism and Pathogenesis of Egg Infections
    Article Snippet: CAMs were extracted, the two washers disconnected from the forceps and the CAMs including the washers were fitted into an embedding cassette and fixed with a clip. .. Biotinylated secondary antibodies were a goat-anti-mouse Fab-fragment (1:200, Jackson Immunoresearch, 115-067-003, Cambridge, UK) and a goat anti-rabbit antibody (1:200, Vector, BA-1000, Burlingame, CA, USA).

    Plasmid Preparation:

    Article Title: Effects of tumor grade and dexamethasone on myeloid cells in patients with glioma
    Article Snippet: A solution of 5% goat serum (Jackson Immuno #005–000-121) + 0.3% Triton X (“Tx”, Thermo Fisher #BP151100) in PBS + Avidin/Biotin solution (Vector #SP-2001) was applied to block nonspecific protein, endogenous biotin and avidin binding sites. .. A biotinylated anti-mouse secondary antibody (Jackson Immuno #115–067-003) was applied for 30 minutes.

    Article Title: Tracking Modified Vaccinia Virus Ankara in the Chicken Embryo: In Vivo Tropism and Pathogenesis of Egg Infections
    Article Snippet: .. Biotinylated secondary antibodies were a goat-anti-mouse Fab-fragment (1:200, Jackson Immunoresearch, 115-067-003, Cambridge, UK) and a goat anti-rabbit antibody (1:200, Vector, BA-1000, Burlingame, CA, USA). .. Peroxidase-complexed avidin-biotin (ABC-HRP, Vector, PK-6100) and diaminobenzidine were used as visualization and hemalum as counterstain.

    Software:

    Article Title: MicroRNA-132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras-MAPK pathway
    Article Snippet: Anti-RASA1 antibody was diluted in 0.5% BSA in TBST, applied on the sections overnight at 4°C, followed by biotin-sp-conjugated affini-pure Fab fragment goat anti-mouse IgG(H+L) (1:500, 115-067-003; Jackson Immunoresearch Laboratory) and streptavidin-conjugated Alexa 555. .. Images were taken by Zeiss LSM700 and analysed using ZEN 2012 software (Zeiss).

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