biotin sp affinipure fab fragment donkey anti goat igg  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Fab Fragment Donkey Anti Goat IgG
    Description:
    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion including the hinge region These antibodies are monovalent containing only a single antigen binding site The molecular weight of Fab fragments is about 50 kDa Based on antigen binding assay and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    705-067-003
    Price:
    97
    Purity:
    The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Donkey
    Quantity:
    0 5
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure fab fragment donkey anti goat igg
    Biotin SP AffiniPure Fab Fragment Donkey Anti Goat IgG
    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion including the hinge region These antibodies are monovalent containing only a single antigen binding site The molecular weight of Fab fragments is about 50 kDa Based on antigen binding assay and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure fab fragment donkey anti goat igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure fab fragment donkey anti goat igg - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Article Snippet: Paragraph title: Immunohistochemistry ... Following blocking, primary antibody against SOST (R & D Systems, AF1589, 1:100) was incubated overnight at 4 °C followed by donkey-anti-goat-biotin secondary (Jackson ImmunoResearch, 705-067-003, 1:400).

    Amplification:

    Article Title: Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Article Snippet: A modified signal amplification protocol was used to visualize SOST. .. Following blocking, primary antibody against SOST (R & D Systems, AF1589, 1:100) was incubated overnight at 4 °C followed by donkey-anti-goat-biotin secondary (Jackson ImmunoResearch, 705-067-003, 1:400).

    Blocking Assay:

    Article Title: Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Article Snippet: .. Following blocking, primary antibody against SOST (R & D Systems, AF1589, 1:100) was incubated overnight at 4 °C followed by donkey-anti-goat-biotin secondary (Jackson ImmunoResearch, 705-067-003, 1:400). .. The biotinylated secondary was detected using Vectastain Elite ABC kit (Vector Laboratories, PK-6100) and signal was amplified by Alexa Fluor 488 Tyramide reagent (Thermo Fisher, B40853).

    Incubation:

    Article Title: Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Article Snippet: .. Following blocking, primary antibody against SOST (R & D Systems, AF1589, 1:100) was incubated overnight at 4 °C followed by donkey-anti-goat-biotin secondary (Jackson ImmunoResearch, 705-067-003, 1:400). .. The biotinylated secondary was detected using Vectastain Elite ABC kit (Vector Laboratories, PK-6100) and signal was amplified by Alexa Fluor 488 Tyramide reagent (Thermo Fisher, B40853).

    Imaging:

    Article Title: Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Article Snippet: Following blocking, primary antibody against SOST (R & D Systems, AF1589, 1:100) was incubated overnight at 4 °C followed by donkey-anti-goat-biotin secondary (Jackson ImmunoResearch, 705-067-003, 1:400). .. To minimize imaging complications from autofluorescence in postnatal and adult tissues, Hoxa11eGFP was visualized using chicken-anti-GFP (Abcam, ab13970, 1:2000) and donkey-anti-chicken-Alexa Fluor 488 (Invitrogen, A11039, 1:1000) in combination with aforementioned antibodies. tdTomato expression was dim at 24–72 h post-tamoxifen induction and was visualized using rabbit-anti-RFP (Rockland, 600401379, 1:1000) and donkey-anti-rabbit-Alexa Fluor 555 (Invitrogen, A31572, 1:1000).

    Expressing:

    Article Title: Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Article Snippet: Following blocking, primary antibody against SOST (R & D Systems, AF1589, 1:100) was incubated overnight at 4 °C followed by donkey-anti-goat-biotin secondary (Jackson ImmunoResearch, 705-067-003, 1:400). .. To minimize imaging complications from autofluorescence in postnatal and adult tissues, Hoxa11eGFP was visualized using chicken-anti-GFP (Abcam, ab13970, 1:2000) and donkey-anti-chicken-Alexa Fluor 488 (Invitrogen, A11039, 1:1000) in combination with aforementioned antibodies. tdTomato expression was dim at 24–72 h post-tamoxifen induction and was visualized using rabbit-anti-RFP (Rockland, 600401379, 1:1000) and donkey-anti-rabbit-Alexa Fluor 555 (Invitrogen, A31572, 1:1000).

    Modification:

    Article Title: Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Article Snippet: A modified signal amplification protocol was used to visualize SOST. .. Following blocking, primary antibody against SOST (R & D Systems, AF1589, 1:100) was incubated overnight at 4 °C followed by donkey-anti-goat-biotin secondary (Jackson ImmunoResearch, 705-067-003, 1:400).

    Plasmid Preparation:

    Article Title: Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
    Article Snippet: Following blocking, primary antibody against SOST (R & D Systems, AF1589, 1:100) was incubated overnight at 4 °C followed by donkey-anti-goat-biotin secondary (Jackson ImmunoResearch, 705-067-003, 1:400). .. The biotinylated secondary was detected using Vectastain Elite ABC kit (Vector Laboratories, PK-6100) and signal was amplified by Alexa Fluor 488 Tyramide reagent (Thermo Fisher, B40853).

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  • News
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  • 99
    Jackson Immuno biotin sp affinipure fab fragment donkey anti goat igg
    Biotin Sp Affinipure Fab Fragment Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure fab fragment donkey anti goat igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure fab fragment donkey anti goat igg - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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