biotin sp affinipure f ab 2 fragment rabbit anti goat igg  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure F ab 2 Fragment Rabbit Anti Goat IgG
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    305-066-003
    Price:
    129
    Purity:
    The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Rabbit
    Quantity:
    0 5
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    Structured Review

    Jackson Immuno biotin sp affinipure f ab 2 fragment rabbit anti goat igg
    Biotin SP AffiniPure F ab 2 Fragment Rabbit Anti Goat IgG
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment rabbit anti goat igg/product/Jackson Immuno
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment rabbit anti goat igg - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Retained Features of Embryonic Metabolism in the Adult MRL Mouse
    Article Snippet: Paragraph title: Immunohistochemistry ... The sections were reacted with secondary antibodies: the anti-Islet-1 was detected by Biotin-SP-AffiniPure F(ab1 )2 fragment rabbit anti-goat IgG (H+L) (305-066-003; Jackson ImmunoResearch, 1:2000), the anti Nanog was detected by Alexa Fluor 568-conjugated goat anti-rabbit IgG antibody ( ; Molecular Probe, 1:800) and anti-Sox2 was detected by biotinylated monoclonal Anti-Rabbit IgG (B5283; Sigma, 1:1000), incubated at RT for 1 hr.

    Incubation:

    Article Title: Retained Features of Embryonic Metabolism in the Adult MRL Mouse
    Article Snippet: .. The sections were reacted with secondary antibodies: the anti-Islet-1 was detected by Biotin-SP-AffiniPure F(ab1 )2 fragment rabbit anti-goat IgG (H+L) (305-066-003; Jackson ImmunoResearch, 1:2000), the anti Nanog was detected by Alexa Fluor 568-conjugated goat anti-rabbit IgG antibody ( ; Molecular Probe, 1:800) and anti-Sox2 was detected by biotinylated monoclonal Anti-Rabbit IgG (B5283; Sigma, 1:1000), incubated at RT for 1 hr. .. After washes with PBS, the sections were reacted with the reagents of the Vectastain ABC Kit (PK-6100; Vector) at RT for 1 hr.

    Binding Assay:

    Article Title: Retained Features of Embryonic Metabolism in the Adult MRL Mouse
    Article Snippet: The tissue sections were then treated with 3% H2 O2 and non-specific binding was blocked with 5% Normal Rabbit Serum (for Anti-Islet-1) and 4% BSA (for Anti-Sox2 and Anti-Nanog). .. The sections were reacted with secondary antibodies: the anti-Islet-1 was detected by Biotin-SP-AffiniPure F(ab1 )2 fragment rabbit anti-goat IgG (H+L) (305-066-003; Jackson ImmunoResearch, 1:2000), the anti Nanog was detected by Alexa Fluor 568-conjugated goat anti-rabbit IgG antibody ( ; Molecular Probe, 1:800) and anti-Sox2 was detected by biotinylated monoclonal Anti-Rabbit IgG (B5283; Sigma, 1:1000), incubated at RT for 1 hr.

    Plasmid Preparation:

    Article Title: Retained Features of Embryonic Metabolism in the Adult MRL Mouse
    Article Snippet: The sections were reacted with secondary antibodies: the anti-Islet-1 was detected by Biotin-SP-AffiniPure F(ab1 )2 fragment rabbit anti-goat IgG (H+L) (305-066-003; Jackson ImmunoResearch, 1:2000), the anti Nanog was detected by Alexa Fluor 568-conjugated goat anti-rabbit IgG antibody ( ; Molecular Probe, 1:800) and anti-Sox2 was detected by biotinylated monoclonal Anti-Rabbit IgG (B5283; Sigma, 1:1000), incubated at RT for 1 hr. .. After washes with PBS, the sections were reacted with the reagents of the Vectastain ABC Kit (PK-6100; Vector) at RT for 1 hr.

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  • 97
    Jackson Immuno biotin sp affinipure f ab 2 fragment rabbit anti goat igg f ab 2 fragment specific
    Biotin Sp Affinipure F Ab 2 Fragment Rabbit Anti Goat Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment rabbit anti goat igg f ab 2 fragment specific/product/Jackson Immuno
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment rabbit anti goat igg f ab 2 fragment specific - by Bioz Stars, 2020-04
    97/100 stars
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    99
    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti rabbit igg
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti rabbit igg/product/Jackson Immuno
    Average 99 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment goat anti rabbit igg - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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