biotin sp affinipure rabbit anti human igm fc5µ fragment specific  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Rabbit Anti Human IgM Fc5µ Fragment Specific
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG IgA or the light chains of human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with mouse serum proteins but it may cross react with IgM from other species
    Catalog Number:
    309-065-095
    Price:
    198
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Rabbit
    Quantity:
    1 0
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    Structured Review

    Jackson Immuno biotin sp affinipure rabbit anti human igm fc5µ fragment specific
    Biotin SP AffiniPure Rabbit Anti Human IgM Fc5µ Fragment Specific
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG IgA or the light chains of human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with mouse serum proteins but it may cross react with IgM from other species
    https://www.bioz.com/result/biotin sp affinipure rabbit anti human igm fc5µ fragment specific/product/Jackson Immuno
    Average 85 stars, based on 1224 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure rabbit anti human igm fc5µ fragment specific - by Bioz Stars, 2020-09
    85/100 stars

    Images

    1) Product Images from "The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *"

    Article Title: The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.357640

    c-Cbl mediates Ag·BCR ubiquitination and processing. Upper panel , B cells were pulsed with anti-human IgM-btn for the indicated time (minutes) at 37 °C. The cells were lysed and ubiquitinated ligand-BCR complexes isolated by ubiquitin
    Figure Legend Snippet: c-Cbl mediates Ag·BCR ubiquitination and processing. Upper panel , B cells were pulsed with anti-human IgM-btn for the indicated time (minutes) at 37 °C. The cells were lysed and ubiquitinated ligand-BCR complexes isolated by ubiquitin

    Techniques Used: Isolation

    2) Product Images from "The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells"

    Article Title: The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20160579

    Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes (IgG2a, IgG2b, and IgG1) were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.
    Figure Legend Snippet: Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes (IgG2a, IgG2b, and IgG1) were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.

    Techniques Used: Mouse Assay, Functional Assay, Concentration Assay

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    CtB Assay:

    Article Title: Physiological-Range Temperature Changes Modulate Cognate Antigen Processing and Presentation Mediated by Lipid Raft-Restricted Ubiquitinated B Cell Receptor Molecules
    Article Snippet: .. The following reagents were used for this study: hen egg lysozyme (HEL, catalog no. L-6876; Sigma, St Louis, MO), Aw3.18 mAb recognizing peptide residues 48–62 of HEL bound to MHC II I-Ak (murine IgG1, Catalog no. CRL-2826 ATCC, Manassas, VA), Goat anti-mouse IgG F(ab′)2 (Catalog no. .115-006-006; Jackson ImmunoResearch, West Grove, PA), FITC Rat anti-mouse IgG1 (A85-1, Catalog no. 553443; BD Pharmingen, San Jose, CA), FITC anti-mouse I-Ak (10-3.6, IgG2a; Catalog no. 553540; BD Pharmingen), FITC anti-mouse IgMa (DS-1, IgG1; Catalog no. 553516; BD Pharmingen), PE anti-CD45R/B220 (RA3–6B2, IgG2a; Catalog no. 553090; BD Pharmingen), Goat anti-humanIgM F(ab′)2 (Catalog no. 109-006-129; Jackson ImmunoResearch), Rabbit anti-human IgM-btn (Catalog no. 309-065-095; Jackson ImmunoResearch), Biotin anti-mouse IgMa (Catalog no. 553515; BD Pharmingen), Anti-Mouse IgMb -btn(AF6–78, catlog no.553519; BD Pharmingen), Cholera Toxin B (CTB, catalog no. 227039; Calbiochem), Horseradish peroxidase conjugated (HRP-CTB, catalog no. C3741; Sigma), PP1 and PP2 (Src Family Tyrosine Kinase Inhibitor, catalog no. BML-EI275 and BML-EI297 respectively; Enzo Life Sciences), anti-GAPDH, mouse monoclonal 6C5 (catalog no. AM4300; Ambion). .. Cells were cultured in bicarbonate-free RPMI 1640 media (Sigma ) supplemented with 10% FBS and 50 μM 2-mercaptoethanol.

    Electron Microscopy:

    Article Title: Cis and Trans Regulatory Mechanisms Control AP2-Mediated B Cell Receptor Endocytosis via Select Tyrosine-Based Motifs
    Article Snippet: .. In brief, A20µWT cells or B10.Br-derived splenocytes were stimulated with rabbit anti-human IgM-btn (309-065-095; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) or mouse anti-mouse IgMb -btn (AF6-78; BD Pharmingen, San Jose, CA, USA), respectively, plus goat anti-btn-15 nm gold (25253; Electron Microscopy Services [EMS], Hatfield, PA, USA) for 5 minutes at 37o C, then cooled. .. The stained cells were then pressed between alcian blue-coated coverslips and lysine-coated nickel EM grids (FCF300-Ni-50; EMS) and quickly separated.

    Article Title: The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *
    Article Snippet: .. The following reagents were used for this study: hen egg lysozyme (HEL, catalog no. l -6876, Sigma), HEL46–61 -I-Ak -specific monoclonal antibody C4H3 , goat anti-mouse IgG F(ab′)2 (catalog no. 115-006-006, Jackson ImmunoResearch, West Grove, PA), FITC rat anti-mouse IgG1 (A85-1, catalog no. 553443, BD Biosciences), FITC anti-mouse I-Ak (10-3.6, IgG2a , catalog no. 55352, BD Biosciences), FITC anti-mouse IgMa (DS-1, IgG1 , catalog no. 553516, BD Biosciences), PE anti-CD45R/B220 (RA3-6B2, IgG2a , catalog no. 553090, BD Biosciences), goat anti-human IgM F(ab′)2 (catalog no. 109-006-129, Jackson ImmunoResearch), rabbit anti-human IgM-btn (catalog no. 309-065-095, Jackson ImmunoResearch), biotin anti-mouse IgMa (catalog no. 553515, BD Biosciences), c-Cbl antibody (catalog no. 2747, Cell Signaling Technology), purified mouse anti-c-Cbl (catalog no. 610442, BD Biosciences), Cbl-b (clone G1, catalog no. sc-8006), Cbl-b (clone C20, catalogue no. sc-1435), purified rat anti-mouse CD107b (LAMP-2) monoclonal antibody (BD Biosciences, catalog no. 558756), SA-Alexa Fluor 594 (Molecular Probes, catalog no. ); Alexa Fluor 647 anti-rat IgG (H+L) (Molecular Probes, catalog no. A-21247), fluoro-gel (Electron Microscopy Sciences, catalog no. 17985-10), RNeasy mini kit (Qiagen, catalog no. 74104), Transcriptor First Strand cDNA synthesis kit (Roche, catalog no. 04 379 012 001), and AccuPrime TaqDNA polymerase (Invitrogen, catalog no. 12339016). ..

    Purification:

    Article Title: The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *
    Article Snippet: .. The following reagents were used for this study: hen egg lysozyme (HEL, catalog no. l -6876, Sigma), HEL46–61 -I-Ak -specific monoclonal antibody C4H3 , goat anti-mouse IgG F(ab′)2 (catalog no. 115-006-006, Jackson ImmunoResearch, West Grove, PA), FITC rat anti-mouse IgG1 (A85-1, catalog no. 553443, BD Biosciences), FITC anti-mouse I-Ak (10-3.6, IgG2a , catalog no. 55352, BD Biosciences), FITC anti-mouse IgMa (DS-1, IgG1 , catalog no. 553516, BD Biosciences), PE anti-CD45R/B220 (RA3-6B2, IgG2a , catalog no. 553090, BD Biosciences), goat anti-human IgM F(ab′)2 (catalog no. 109-006-129, Jackson ImmunoResearch), rabbit anti-human IgM-btn (catalog no. 309-065-095, Jackson ImmunoResearch), biotin anti-mouse IgMa (catalog no. 553515, BD Biosciences), c-Cbl antibody (catalog no. 2747, Cell Signaling Technology), purified mouse anti-c-Cbl (catalog no. 610442, BD Biosciences), Cbl-b (clone G1, catalog no. sc-8006), Cbl-b (clone C20, catalogue no. sc-1435), purified rat anti-mouse CD107b (LAMP-2) monoclonal antibody (BD Biosciences, catalog no. 558756), SA-Alexa Fluor 594 (Molecular Probes, catalog no. ); Alexa Fluor 647 anti-rat IgG (H+L) (Molecular Probes, catalog no. A-21247), fluoro-gel (Electron Microscopy Sciences, catalog no. 17985-10), RNeasy mini kit (Qiagen, catalog no. 74104), Transcriptor First Strand cDNA synthesis kit (Roche, catalog no. 04 379 012 001), and AccuPrime TaqDNA polymerase (Invitrogen, catalog no. 12339016). ..

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