biotin sp affinipure f ab 2 fragment goat anti rabbit igg  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure F ab 2 Fragment Goat Anti Rabbit IgG
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    111-066-003
    Price:
    97
    Purity:
    The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    0 5
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    Structured Review

    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti rabbit igg
    Biotin SP AffiniPure F ab 2 Fragment Goat Anti Rabbit IgG
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti rabbit igg/product/Jackson Immuno
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment goat anti rabbit igg - by Bioz Stars, 2020-05
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: An ultrasensitive fiveplex activity assay for cellular kinases
    Article Snippet: .. After, the wells were washed twice with Luminex wash buffer, and the beads were resuspended in 50 µL Luminex block buffer containing a 1:10,000 dilution of biotinylated anti-rabbit antibody (Jackson Immunoresearch #111-066-144). .. After, the plate was washed twice with Luminex wash buffer, and the beads were resuspended in 50 µL Luminex block buffer containing a 1:5000 dilution of streptavidin-phycoerythrin (Prozyme #PJ31S).

    Incubation:

    Article Title: Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins
    Article Snippet: .. Most of the cells were also positive for consortin, as evaluated by immmunolabeling with anti-consortin rabbit polyclonal antibody, diluted 1:50 (overnight incubation at 4°C of cells fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100) and reacted first with a biotin conjugated goat serum against rabbit IgGs (111-066-045, Jackson ImmunoResearch), diluted 1:200, and then with fluorescein-conjugated streptavidin (016-090-084, Jackson ImmunoResearch), diluted 1:600. .. To minimize the influence of clone-dependent differences, further experiments were carried out on cell populations made in equal parts of three to four different clones (one per transfection procedure) that were pooled for each type of Cx43-expressing cell.

    other:

    Article Title: Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells
    Article Snippet: Secondary antibodies were the following: Biotin-SP goat anti-rabbit IgG (111-066-003, Jackson ImmunoResearch), streptavidin Cy5 (SA1011, Invitrogen), anti-mouse IgG2b Alexa Fluor 568 (A21144, Thermo Scientific), anti-chicken Alexa Fluor 488 (A11039, Thermo Scientific) and goat anti-rabbit IgG Alexa Fluor 568 (A11036, Thermo Scientific), all diluted at 1:500.

    Labeling:

    Article Title: Pharmacological inhibition of lysosomes activates the MTORC1 signaling pathway in chondrocytes in an autophagy-independent manner
    Article Snippet: .. The following antibodies were used: anti-p-RPS6 (Cell Signaling Technology, 4858), anti-AKT (Cell Signaling Technology, 9272), anti-p-AKT (Ser473; Cell Signaling Technology, 4060), anti-RPS6 (5G10; Cell Signaling Technology, 2217), anti-GAPDH (Cell Signaling Technology, 2118), anti-EIF4EBP1 (53H11; Cell Signaling Technology, 9644), anti-phospho-EIF4EBP1 (Thr37/46; Cell Signaling Technology, 2855), anti-MAP1LC3A (MBL, PM036), LAMP2 (Santa Cruz Biotechnology, sc-8100), FLCN (Cell Signaling Technology, 3697), anti-ATG5 (Cell Signaling Technology, 8540), anti-SQSTM1 (Progen Bioteknik, GP62-C), anti-COL10A1 (Quartett; GMBH, 1-CO097-05), biotin-conjugated anti-guinea pig, anti-rabbit and anti-mouse secondary antibodies (Jackson ImmunoResearch,106-065-003, 111-066-003 and 115-066-003, respectively), mouse anti-BrdU antibody (Sigma, B8434), biotinylated anti-mouse antibody (Jackson, 115-066-003), streptavidin-conjugated Alexa Fluor 546 (Life Technologies, S11225), fluorescently labeled anti-goat antibody (Jackson, 705165147) and fluorescently labeled anti-rabbit antibody (Jackson, 711545152). .. Additional reagents include the following: Weigerts hematoxylin (Sigma, H3136-25G), Fast Green (Sigma, F7258-25G), Safranin O solution (Sigma Aldrich, S8884-25G), sodium acetate anhydrous (Sigma S-2889), L-(+) tartaric acid (Sigma, T-6521), naphthol AS-BI phosphate (Sigma N-2125), pararosaniline chloride (Sigma, P-3750), sodium nitrite (Sigma, S-2252), 96-well micro-plates (Ibidi inc., 89626), TRIZOL (Life Technologies, 15596-026), High-Capacity RNA to cDNA kit (Life technologies, 4387406), Taqman® Fast Universal Master Mix (Life technologies, 4352042), and the StepOnePlus™ Real-Time PCR System (Life technologies, 4376600).

    Staining:

    Article Title: Localisation of Lactate Transporters in Rat and Rabbit Placentae
    Article Snippet: .. Slides were stained immunohistochemically using the Ventana Discovery XT slide staining system with rabbit anti-human MCT4 (Biorbyt LLC, San Francisco, CA; catalogue number orb137272) as the primary antibody, and goat anti-rabbit as the secondary antibody (Jackson Immunoresearch, reference number 111-066-003). .. The avidin-biotin (ABC) detection method was used followed by diaminobenzidine (DAB) as the chromagen.

    Article Title: Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf
    Article Snippet: .. For detection of pS21-Ezh2 (rabbit polyclonal, Bethyl Laboratories) or pT487-Ezh2 (rabbit polyclonal, abbexa, UK), the surface-stained and fixed cells were first stained with the primary antibody, followed by sequential staining with biotinylated goat anti-rabbit IgG (Cat. No. 111-066-144, Jackson ImmunoResearch Laboratories) and fluorochrome-conjugated streptavidin. .. For more accurate measurement of the impact of Ezh2 deficiency on Bcl6 expression, Bcl6 expression was specifically analyzed on Ezh2-negative Ezh2 –/– TFH cells (as in Figs. b, g, , Supplementary Figures d and ), excluding about 10% of cells that escaped deletion and retained Ezh2 protein.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: BMP4 is essential for lens induction in the mouse embryo
    Article Snippet: .. Secondary antibody was an anti-rabbit IgG antibody (goat) conjugated with biotin (Jackson ImmunoResearch Laboratories; Cat.#111-066-003), to which peroxidase-conjugated streptoavidin (Vectastain ABC kit; Vector Laboratories) was then bound. ..

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  • 93
    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti rabbit igg
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti rabbit igg/product/Jackson Immuno
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment goat anti rabbit igg - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    88
    Jackson Immuno biotin sp affinipure f ab 2 fragment rabbit anti goat igg f ab 2 fragment specific
    Biotin Sp Affinipure F Ab 2 Fragment Rabbit Anti Goat Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment rabbit anti goat igg f ab 2 fragment specific/product/Jackson Immuno
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment rabbit anti goat igg f ab 2 fragment specific - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    90
    Jackson Immuno biotin conjugated f
    Biotin Conjugated F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated f/product/Jackson Immuno
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated f - by Bioz Stars, 2020-05
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    88
    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti rabbit igg f ab 2 fragment specific
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Rabbit Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti rabbit igg f ab 2 fragment specific/product/Jackson Immuno
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment goat anti rabbit igg f ab 2 fragment specific - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

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