biotin sp affinipure f ab 2 fragment donkey anti rabbit igg  (Jackson Immuno)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Biotin SP AffiniPure F ab 2 Fragment Donkey Anti Rabbit IgG
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine chicken goat guinea pig syrian hamster horse human mouse rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    711-066-152
    Price:
    137
    Purity:
    The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Donkey
    Quantity:
    0 3
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure f ab 2 fragment donkey anti rabbit igg
    Biotin SP AffiniPure F ab 2 Fragment Donkey Anti Rabbit IgG
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine chicken goat guinea pig syrian hamster horse human mouse rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment donkey anti rabbit igg/product/Jackson Immuno
    Average 94 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment donkey anti rabbit igg - by Bioz Stars, 2020-07
    94/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Early loss of Crebbp confers malignant stem cell properties on lymphoid progenitors
    Article Snippet: .. Staining was performed using anti-Ki-67 (abcam ab16667, clone SP6, 1:50) followed by biotin-SP-conjugated donkey anti-Rabbit IgG (711-066-152; Jackson ImmunoResearch Laboratories. .. Inc) and Vectastain elite ABC kit (Vector labs, Pk-6100).

    Incubation:

    Article Title: Defining a novel leptin–melanocortin–kisspeptin pathway involved in the metabolic control of puberty
    Article Snippet: .. One set of free-floating sections from each animal was: (i ) washed (3 × 10 min) in Tris-buffered saline (TBS) (pH 7.6) at room temperature with gentle agitation; (ii ) incubated with a primary rabbit polyclonal anti-mCherry antibody 1:1000 (ab167453, Abcam) at 4 °C for 48 h; (iii ) washed (3 × 10 min) in TBS (pH 7.6) at room temperature; (iv ) incubated with a secondary biotinilated Donkey Anti-Rabbit antibody 1:200 (JAC-711-066-152, Jackson Immunoresearch) at room temperature for 90 min; (v ) washed (3 × 10 min) in TBS (pH 7.6); (vi ) incubated with Texas Red Streptavidin 1:200 (SA-5006, Vector laboratories, Inc.) at room temperature for 90 min; and (vii ) washed (3 × 10 min) in TBS (pH 7.6). .. Thereafter, brain sections were mounted on silane-coated slices, air dried, and cover-slipped with Vectashield HardSet Mounting Medium (Vector Laboratories).

    Article Title: YKL-40 Is Differentially Expressed in Human Embryonic Stem Cells and in Cell Progeny of the Three Germ Layers
    Article Snippet: .. Finally, they were incubated with biotin-SP-conjugated F(ab′)2 fragment donkey anti-rabbit antibodies (711-066-152; Jackson ImmunoResearch) followed by streptavidin-conjugated Dylight 594 (SA5594; Vector Laboratories, Burlingame, CA) and then coverslipped. .. After staining for YKL-40, the sections were subjected to antigen retrieval in TEG buffer.

    Article Title: SSEA‐4 and YKL‐40 positive progenitor subtypes in the subventricular zone of developing human neocortex
    Article Snippet: .. Subsequently, the sections were incubated for 30 min at room temperature with biotin‐SP‐conjugated F(ab')2 fragment donkey anti‐rabbit antibodies (Jackson ImmunoResearch, 711‐066‐152, 1:200) followed by streptavidin‐conjugated DyLight 594 (Vector Laboratories, SA5594, 1:200). .. Finally, a nuclear counterstain with DAPI (4′,6‐diamidino‐2‐phenylindole, Invitrogen, Molecular Probes, D1306, 1:1,000) was added for 3 min, before sections were coverslipped.

    Article Title: Outer brain barriers in rat and human development
    Article Snippet: .. Subsequently, the sections were incubated for 30 min at room temperature with biotin-SP-conjugated F(ab′)2 fragment donkey anti-rabbit antibodies (1:200, 711-066-152, Jackson ImmunoResearch Europe, Suffolk, United Kingdom) followed by streptavidin-conjugated DyLight 594 (1:200, SA5594, Vector Laboratories Europe, Peterborough, United Kingdom). .. Finally, a nuclear counterstain with DAPI (1:1000, D1306, Life Technologies, Carlsbad, CA, USA) was added for 3 min, before sections were coverslipped.

    other:

    Article Title: Cocaine-and Amphetamine Regulated Transcript (CART) Peptide Is Expressed in Precursor Cells and Somatotropes of the Mouse Pituitary Gland
    Article Snippet: The secondary biotin anti-rat IgG (1:200, 711-066-152, Jackson Immunoresearch, Westgrove, PA) was used followed by detection with Alexa Flour 488.

    Article Title: Deletion of OTX2 in neural ectoderm delays anterior pituitary development
    Article Snippet: The secondary biotin anti-rat IgG (1 : 200, 711-066-152, Jackson Immunoresearch, Westgrove, PA, USA), and the TSA kit were used for detection.

    Article Title: In Vivo Detection of Hyperoxia-Induced Pulmonary Endothelial Cell Death Using 99mTc-Duramycin
    Article Snippet: Antibodies to cleaved caspase 3 (Biocare, #CP229B), CD31 (Acris, #AP15436PU-N), keratin 7 (Abcam, #9021), and myeloperoxidase (Abcam, #45977) were used with appropriate secondary antibodies (Jackson Immuno #711-066-152 for CD31, Jackson Immuno #715-066-151 for keratin 7, and Abcam #6829 HRP for MPO) to identify endpoints of interest.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Jackson Immuno biotin sp affinipure f ab 2 fragment donkey anti rabbit igg
    Biotin Sp Affinipure F Ab 2 Fragment Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment donkey anti rabbit igg/product/Jackson Immuno
    Average 94 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment donkey anti rabbit igg - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results