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Jackson Immuno biotin sp affinipure donkey anti rabbit igg
Biotin Sp Affinipure Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin sp affinipure donkey anti rabbit igg/product/Jackson Immuno
Average 99 stars, based on 35 article reviews
Price from $9.99 to $1999.99
biotin sp affinipure donkey anti rabbit igg - by Bioz Stars, 2020-05
99/100 stars

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Related Articles

Radioactivity:

Article Title: Expression of the glutamate-cystine (xc-) exchanger in cultured retinal ganglion cells and regulation by nitric oxide and oxidative stress
Article Snippet: .. Reagents used in this study were obtained from the following sources: Dulbecco's modified Eagle's medium F12 (DMEM:F12) medium, Earl's Balanced Salt Solution, Dulbecco's Phosphate Buffered Saline, Neurobasal medium, TRIzol reagent, penicillin-streptomycin (Gibco-Life Technologies, Rockville, MD); [2,3-3 H]-glutamate (specific radioactivity 50.0 Ci/mmol) (Amersham Bioscience, Arlington Heights, IL), L-[4,5-3H(N)]-leucine (specific radioactivity 60 Ci/mmol) (New England Nuclear, Boston, MA), L-[2,3-3H]-alanine (specific radioactivity 40 Ci/mmol) (Moravek Biochemicals, Brea, CA); 4F2hc (CD98) goat polyclonal IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc, Santa Cruz, CA); Cy-3 conjugated anti-rabbbit IgG, peroxidase-conjugated AffiniPure rabbit anti-goat IgG, Alexa Fluor 488-conjugated anti-goat IgG (In Vitrogen, Carlsbad, CA); Hoeschst dye 33342 (Molecular Probes, Eugene, OR); ECL detection kit (Pierce Biotechnology, Inc., Rockford, IL); Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, CA); Power Block (BioGenex, San Ramon, CA); Glutathione (GSH) Detection Kit (Chemicon International, Temecula, CA); complete Mini Protease Inhibitor Cocktail tablets, GeneAmp RNA PCR Kit (Applied Biosystems/Roche Molecular System, Branchburg, NJ); TaKaRa Taq™ (Takara Bio Inc, Otsu, Shiga, Japan); sense and antisense primers for xCT and 4F2hc (Integrated DNA Technologies, Inc, Coralville, IA); papain (Worthington, Lakewood, NJ), trypsin inhibitor (Roche Applied Science, Mannheim, Germany), rabbit anti-macrophage antiserum (Accurate Chemical Corp., Westbury, NY), AffiniPure donkey anti-rabbit IgG (H+L) (Jackson, ImmunoResearch, Westgrove, PA), purified rat anti-mouse Thy 1.2 antibody (Pharmingen, San Diego, CA), brain-derived neurotrophic factor, ciliary-derived neurotrophic factor (basic fibroblast growth factor (PeproTech Inc., Rocky Hill, NJ), 3-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (SNOG), 3-Morpholinosydnonimine hydrochloride (SIN-1), menadione sodium bisulfite, hydrogen peroxide 30% (W/W) solution, xanthine sodium salt, xanthine oxidase, fetal bovine serum, monoclonal antibody to β-actin and all other chemicals (Sigma-Aldrich Chemical Co., St. Louis, MO); RGC-5 cells were the kind gift of Dr. Neeraj Agarwal, Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, TX. ..

Fluorescence:

Article Title: miR-23b-3p regulates the chemoresistance of gastric cancer cells by targeting ATG12 and HMGB2
Article Snippet: .. Briefly, the cells were incubated with primary antibodies recognizing ATG12 and HMGB2 (rabbit, Abcam, Cambridge, UK) overnight at 4 °C, and then fixed and incubated with AffiniPure donkey anti-rabbit IgG (1 : 200; red fluorescence, Jackson ImmunoResearch Laboratories Inc., Baltimore, MD, USA) as the secondary antibody for 1 h at room temperature. .. DAPI (Beyotime, Shanghai, China) was applied for 5 min to stain the nuclei.

Protease Inhibitor:

Article Title: Expression of the glutamate-cystine (xc-) exchanger in cultured retinal ganglion cells and regulation by nitric oxide and oxidative stress
Article Snippet: .. Reagents used in this study were obtained from the following sources: Dulbecco's modified Eagle's medium F12 (DMEM:F12) medium, Earl's Balanced Salt Solution, Dulbecco's Phosphate Buffered Saline, Neurobasal medium, TRIzol reagent, penicillin-streptomycin (Gibco-Life Technologies, Rockville, MD); [2,3-3 H]-glutamate (specific radioactivity 50.0 Ci/mmol) (Amersham Bioscience, Arlington Heights, IL), L-[4,5-3H(N)]-leucine (specific radioactivity 60 Ci/mmol) (New England Nuclear, Boston, MA), L-[2,3-3H]-alanine (specific radioactivity 40 Ci/mmol) (Moravek Biochemicals, Brea, CA); 4F2hc (CD98) goat polyclonal IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc, Santa Cruz, CA); Cy-3 conjugated anti-rabbbit IgG, peroxidase-conjugated AffiniPure rabbit anti-goat IgG, Alexa Fluor 488-conjugated anti-goat IgG (In Vitrogen, Carlsbad, CA); Hoeschst dye 33342 (Molecular Probes, Eugene, OR); ECL detection kit (Pierce Biotechnology, Inc., Rockford, IL); Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, CA); Power Block (BioGenex, San Ramon, CA); Glutathione (GSH) Detection Kit (Chemicon International, Temecula, CA); complete Mini Protease Inhibitor Cocktail tablets, GeneAmp RNA PCR Kit (Applied Biosystems/Roche Molecular System, Branchburg, NJ); TaKaRa Taq™ (Takara Bio Inc, Otsu, Shiga, Japan); sense and antisense primers for xCT and 4F2hc (Integrated DNA Technologies, Inc, Coralville, IA); papain (Worthington, Lakewood, NJ), trypsin inhibitor (Roche Applied Science, Mannheim, Germany), rabbit anti-macrophage antiserum (Accurate Chemical Corp., Westbury, NY), AffiniPure donkey anti-rabbit IgG (H+L) (Jackson, ImmunoResearch, Westgrove, PA), purified rat anti-mouse Thy 1.2 antibody (Pharmingen, San Diego, CA), brain-derived neurotrophic factor, ciliary-derived neurotrophic factor (basic fibroblast growth factor (PeproTech Inc., Rocky Hill, NJ), 3-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (SNOG), 3-Morpholinosydnonimine hydrochloride (SIN-1), menadione sodium bisulfite, hydrogen peroxide 30% (W/W) solution, xanthine sodium salt, xanthine oxidase, fetal bovine serum, monoclonal antibody to β-actin and all other chemicals (Sigma-Aldrich Chemical Co., St. Louis, MO); RGC-5 cells were the kind gift of Dr. Neeraj Agarwal, Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, TX. ..

Immunolabeling:

Article Title: Rostro-Caudal Analysis of Corpus Callosum Demyelination and Axon Damage Across Disease Stages Refines Diffusion Tensor Imaging Correlations with Pathological Features
Article Snippet: .. MOG and SMI-32 immunolabeling were detected with Cy3-conjugated donkey anti-mouse IgG F(ab′)2 and NF200 was detected using donkey anti-rabbit IgG F(ab′)2 conjugated with FITC (Jackson Immunoresearch, West Grove, PA). .. Immunofluorescence images were acquired on an Olympus IX-70 microscope for low magnification (4x) analysis using a Spot 2 CCD digital camera (Diagnostic Instruments).

Blocking Assay:

Article Title: Expression of the glutamate-cystine (xc-) exchanger in cultured retinal ganglion cells and regulation by nitric oxide and oxidative stress
Article Snippet: .. Reagents used in this study were obtained from the following sources: Dulbecco's modified Eagle's medium F12 (DMEM:F12) medium, Earl's Balanced Salt Solution, Dulbecco's Phosphate Buffered Saline, Neurobasal medium, TRIzol reagent, penicillin-streptomycin (Gibco-Life Technologies, Rockville, MD); [2,3-3 H]-glutamate (specific radioactivity 50.0 Ci/mmol) (Amersham Bioscience, Arlington Heights, IL), L-[4,5-3H(N)]-leucine (specific radioactivity 60 Ci/mmol) (New England Nuclear, Boston, MA), L-[2,3-3H]-alanine (specific radioactivity 40 Ci/mmol) (Moravek Biochemicals, Brea, CA); 4F2hc (CD98) goat polyclonal IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc, Santa Cruz, CA); Cy-3 conjugated anti-rabbbit IgG, peroxidase-conjugated AffiniPure rabbit anti-goat IgG, Alexa Fluor 488-conjugated anti-goat IgG (In Vitrogen, Carlsbad, CA); Hoeschst dye 33342 (Molecular Probes, Eugene, OR); ECL detection kit (Pierce Biotechnology, Inc., Rockford, IL); Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, CA); Power Block (BioGenex, San Ramon, CA); Glutathione (GSH) Detection Kit (Chemicon International, Temecula, CA); complete Mini Protease Inhibitor Cocktail tablets, GeneAmp RNA PCR Kit (Applied Biosystems/Roche Molecular System, Branchburg, NJ); TaKaRa Taq™ (Takara Bio Inc, Otsu, Shiga, Japan); sense and antisense primers for xCT and 4F2hc (Integrated DNA Technologies, Inc, Coralville, IA); papain (Worthington, Lakewood, NJ), trypsin inhibitor (Roche Applied Science, Mannheim, Germany), rabbit anti-macrophage antiserum (Accurate Chemical Corp., Westbury, NY), AffiniPure donkey anti-rabbit IgG (H+L) (Jackson, ImmunoResearch, Westgrove, PA), purified rat anti-mouse Thy 1.2 antibody (Pharmingen, San Diego, CA), brain-derived neurotrophic factor, ciliary-derived neurotrophic factor (basic fibroblast growth factor (PeproTech Inc., Rocky Hill, NJ), 3-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (SNOG), 3-Morpholinosydnonimine hydrochloride (SIN-1), menadione sodium bisulfite, hydrogen peroxide 30% (W/W) solution, xanthine sodium salt, xanthine oxidase, fetal bovine serum, monoclonal antibody to β-actin and all other chemicals (Sigma-Aldrich Chemical Co., St. Louis, MO); RGC-5 cells were the kind gift of Dr. Neeraj Agarwal, Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, TX. ..

Purification:

Article Title: Expression of the glutamate-cystine (xc-) exchanger in cultured retinal ganglion cells and regulation by nitric oxide and oxidative stress
Article Snippet: .. Reagents used in this study were obtained from the following sources: Dulbecco's modified Eagle's medium F12 (DMEM:F12) medium, Earl's Balanced Salt Solution, Dulbecco's Phosphate Buffered Saline, Neurobasal medium, TRIzol reagent, penicillin-streptomycin (Gibco-Life Technologies, Rockville, MD); [2,3-3 H]-glutamate (specific radioactivity 50.0 Ci/mmol) (Amersham Bioscience, Arlington Heights, IL), L-[4,5-3H(N)]-leucine (specific radioactivity 60 Ci/mmol) (New England Nuclear, Boston, MA), L-[2,3-3H]-alanine (specific radioactivity 40 Ci/mmol) (Moravek Biochemicals, Brea, CA); 4F2hc (CD98) goat polyclonal IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc, Santa Cruz, CA); Cy-3 conjugated anti-rabbbit IgG, peroxidase-conjugated AffiniPure rabbit anti-goat IgG, Alexa Fluor 488-conjugated anti-goat IgG (In Vitrogen, Carlsbad, CA); Hoeschst dye 33342 (Molecular Probes, Eugene, OR); ECL detection kit (Pierce Biotechnology, Inc., Rockford, IL); Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, CA); Power Block (BioGenex, San Ramon, CA); Glutathione (GSH) Detection Kit (Chemicon International, Temecula, CA); complete Mini Protease Inhibitor Cocktail tablets, GeneAmp RNA PCR Kit (Applied Biosystems/Roche Molecular System, Branchburg, NJ); TaKaRa Taq™ (Takara Bio Inc, Otsu, Shiga, Japan); sense and antisense primers for xCT and 4F2hc (Integrated DNA Technologies, Inc, Coralville, IA); papain (Worthington, Lakewood, NJ), trypsin inhibitor (Roche Applied Science, Mannheim, Germany), rabbit anti-macrophage antiserum (Accurate Chemical Corp., Westbury, NY), AffiniPure donkey anti-rabbit IgG (H+L) (Jackson, ImmunoResearch, Westgrove, PA), purified rat anti-mouse Thy 1.2 antibody (Pharmingen, San Diego, CA), brain-derived neurotrophic factor, ciliary-derived neurotrophic factor (basic fibroblast growth factor (PeproTech Inc., Rocky Hill, NJ), 3-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (SNOG), 3-Morpholinosydnonimine hydrochloride (SIN-1), menadione sodium bisulfite, hydrogen peroxide 30% (W/W) solution, xanthine sodium salt, xanthine oxidase, fetal bovine serum, monoclonal antibody to β-actin and all other chemicals (Sigma-Aldrich Chemical Co., St. Louis, MO); RGC-5 cells were the kind gift of Dr. Neeraj Agarwal, Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, TX. ..

Polymerase Chain Reaction:

Article Title: Expression of the glutamate-cystine (xc-) exchanger in cultured retinal ganglion cells and regulation by nitric oxide and oxidative stress
Article Snippet: .. Reagents used in this study were obtained from the following sources: Dulbecco's modified Eagle's medium F12 (DMEM:F12) medium, Earl's Balanced Salt Solution, Dulbecco's Phosphate Buffered Saline, Neurobasal medium, TRIzol reagent, penicillin-streptomycin (Gibco-Life Technologies, Rockville, MD); [2,3-3 H]-glutamate (specific radioactivity 50.0 Ci/mmol) (Amersham Bioscience, Arlington Heights, IL), L-[4,5-3H(N)]-leucine (specific radioactivity 60 Ci/mmol) (New England Nuclear, Boston, MA), L-[2,3-3H]-alanine (specific radioactivity 40 Ci/mmol) (Moravek Biochemicals, Brea, CA); 4F2hc (CD98) goat polyclonal IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc, Santa Cruz, CA); Cy-3 conjugated anti-rabbbit IgG, peroxidase-conjugated AffiniPure rabbit anti-goat IgG, Alexa Fluor 488-conjugated anti-goat IgG (In Vitrogen, Carlsbad, CA); Hoeschst dye 33342 (Molecular Probes, Eugene, OR); ECL detection kit (Pierce Biotechnology, Inc., Rockford, IL); Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, CA); Power Block (BioGenex, San Ramon, CA); Glutathione (GSH) Detection Kit (Chemicon International, Temecula, CA); complete Mini Protease Inhibitor Cocktail tablets, GeneAmp RNA PCR Kit (Applied Biosystems/Roche Molecular System, Branchburg, NJ); TaKaRa Taq™ (Takara Bio Inc, Otsu, Shiga, Japan); sense and antisense primers for xCT and 4F2hc (Integrated DNA Technologies, Inc, Coralville, IA); papain (Worthington, Lakewood, NJ), trypsin inhibitor (Roche Applied Science, Mannheim, Germany), rabbit anti-macrophage antiserum (Accurate Chemical Corp., Westbury, NY), AffiniPure donkey anti-rabbit IgG (H+L) (Jackson, ImmunoResearch, Westgrove, PA), purified rat anti-mouse Thy 1.2 antibody (Pharmingen, San Diego, CA), brain-derived neurotrophic factor, ciliary-derived neurotrophic factor (basic fibroblast growth factor (PeproTech Inc., Rocky Hill, NJ), 3-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (SNOG), 3-Morpholinosydnonimine hydrochloride (SIN-1), menadione sodium bisulfite, hydrogen peroxide 30% (W/W) solution, xanthine sodium salt, xanthine oxidase, fetal bovine serum, monoclonal antibody to β-actin and all other chemicals (Sigma-Aldrich Chemical Co., St. Louis, MO); RGC-5 cells were the kind gift of Dr. Neeraj Agarwal, Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, TX. ..

Incubation:

Article Title: miR-23b-3p regulates the chemoresistance of gastric cancer cells by targeting ATG12 and HMGB2
Article Snippet: .. Briefly, the cells were incubated with primary antibodies recognizing ATG12 and HMGB2 (rabbit, Abcam, Cambridge, UK) overnight at 4 °C, and then fixed and incubated with AffiniPure donkey anti-rabbit IgG (1 : 200; red fluorescence, Jackson ImmunoResearch Laboratories Inc., Baltimore, MD, USA) as the secondary antibody for 1 h at room temperature. .. DAPI (Beyotime, Shanghai, China) was applied for 5 min to stain the nuclei.

Modification:

Article Title: Expression of the glutamate-cystine (xc-) exchanger in cultured retinal ganglion cells and regulation by nitric oxide and oxidative stress
Article Snippet: .. Reagents used in this study were obtained from the following sources: Dulbecco's modified Eagle's medium F12 (DMEM:F12) medium, Earl's Balanced Salt Solution, Dulbecco's Phosphate Buffered Saline, Neurobasal medium, TRIzol reagent, penicillin-streptomycin (Gibco-Life Technologies, Rockville, MD); [2,3-3 H]-glutamate (specific radioactivity 50.0 Ci/mmol) (Amersham Bioscience, Arlington Heights, IL), L-[4,5-3H(N)]-leucine (specific radioactivity 60 Ci/mmol) (New England Nuclear, Boston, MA), L-[2,3-3H]-alanine (specific radioactivity 40 Ci/mmol) (Moravek Biochemicals, Brea, CA); 4F2hc (CD98) goat polyclonal IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc, Santa Cruz, CA); Cy-3 conjugated anti-rabbbit IgG, peroxidase-conjugated AffiniPure rabbit anti-goat IgG, Alexa Fluor 488-conjugated anti-goat IgG (In Vitrogen, Carlsbad, CA); Hoeschst dye 33342 (Molecular Probes, Eugene, OR); ECL detection kit (Pierce Biotechnology, Inc., Rockford, IL); Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, CA); Power Block (BioGenex, San Ramon, CA); Glutathione (GSH) Detection Kit (Chemicon International, Temecula, CA); complete Mini Protease Inhibitor Cocktail tablets, GeneAmp RNA PCR Kit (Applied Biosystems/Roche Molecular System, Branchburg, NJ); TaKaRa Taq™ (Takara Bio Inc, Otsu, Shiga, Japan); sense and antisense primers for xCT and 4F2hc (Integrated DNA Technologies, Inc, Coralville, IA); papain (Worthington, Lakewood, NJ), trypsin inhibitor (Roche Applied Science, Mannheim, Germany), rabbit anti-macrophage antiserum (Accurate Chemical Corp., Westbury, NY), AffiniPure donkey anti-rabbit IgG (H+L) (Jackson, ImmunoResearch, Westgrove, PA), purified rat anti-mouse Thy 1.2 antibody (Pharmingen, San Diego, CA), brain-derived neurotrophic factor, ciliary-derived neurotrophic factor (basic fibroblast growth factor (PeproTech Inc., Rocky Hill, NJ), 3-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (SNOG), 3-Morpholinosydnonimine hydrochloride (SIN-1), menadione sodium bisulfite, hydrogen peroxide 30% (W/W) solution, xanthine sodium salt, xanthine oxidase, fetal bovine serum, monoclonal antibody to β-actin and all other chemicals (Sigma-Aldrich Chemical Co., St. Louis, MO); RGC-5 cells were the kind gift of Dr. Neeraj Agarwal, Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, TX. ..

IA:

Article Title: Expression of the glutamate-cystine (xc-) exchanger in cultured retinal ganglion cells and regulation by nitric oxide and oxidative stress
Article Snippet: .. Reagents used in this study were obtained from the following sources: Dulbecco's modified Eagle's medium F12 (DMEM:F12) medium, Earl's Balanced Salt Solution, Dulbecco's Phosphate Buffered Saline, Neurobasal medium, TRIzol reagent, penicillin-streptomycin (Gibco-Life Technologies, Rockville, MD); [2,3-3 H]-glutamate (specific radioactivity 50.0 Ci/mmol) (Amersham Bioscience, Arlington Heights, IL), L-[4,5-3H(N)]-leucine (specific radioactivity 60 Ci/mmol) (New England Nuclear, Boston, MA), L-[2,3-3H]-alanine (specific radioactivity 40 Ci/mmol) (Moravek Biochemicals, Brea, CA); 4F2hc (CD98) goat polyclonal IgG, goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Inc, Santa Cruz, CA); Cy-3 conjugated anti-rabbbit IgG, peroxidase-conjugated AffiniPure rabbit anti-goat IgG, Alexa Fluor 488-conjugated anti-goat IgG (In Vitrogen, Carlsbad, CA); Hoeschst dye 33342 (Molecular Probes, Eugene, OR); ECL detection kit (Pierce Biotechnology, Inc., Rockford, IL); Bio-Rad Protein Assay Reagent (Bio-Rad, Hercules, CA); Power Block (BioGenex, San Ramon, CA); Glutathione (GSH) Detection Kit (Chemicon International, Temecula, CA); complete Mini Protease Inhibitor Cocktail tablets, GeneAmp RNA PCR Kit (Applied Biosystems/Roche Molecular System, Branchburg, NJ); TaKaRa Taq™ (Takara Bio Inc, Otsu, Shiga, Japan); sense and antisense primers for xCT and 4F2hc (Integrated DNA Technologies, Inc, Coralville, IA); papain (Worthington, Lakewood, NJ), trypsin inhibitor (Roche Applied Science, Mannheim, Germany), rabbit anti-macrophage antiserum (Accurate Chemical Corp., Westbury, NY), AffiniPure donkey anti-rabbit IgG (H+L) (Jackson, ImmunoResearch, Westgrove, PA), purified rat anti-mouse Thy 1.2 antibody (Pharmingen, San Diego, CA), brain-derived neurotrophic factor, ciliary-derived neurotrophic factor (basic fibroblast growth factor (PeproTech Inc., Rocky Hill, NJ), 3-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (SNOG), 3-Morpholinosydnonimine hydrochloride (SIN-1), menadione sodium bisulfite, hydrogen peroxide 30% (W/W) solution, xanthine sodium salt, xanthine oxidase, fetal bovine serum, monoclonal antibody to β-actin and all other chemicals (Sigma-Aldrich Chemical Co., St. Louis, MO); RGC-5 cells were the kind gift of Dr. Neeraj Agarwal, Department of Anatomy and Cell Biology, University of North Texas Health Science Center, Fort Worth, TX. ..

Staining:

Article Title: Enhanced Susceptibility to Endotoxic Shock and Impaired STAT3 Signaling in CD31-Deficient Mice
Article Snippet: .. Splenocytes were prepared as described, fixed in 2% PFA, then stained with 0.5 μg of polyclonal anti-mouse TLR4 (Imgenex) in Hanks’ buffered salt solution plus 1% BSA for 1 hour on ice, then, following two washes, in donkey anti-rabbit IgG F(ab′)2 PE conjugate (Jackson Immunoresearch, West Grove, PA). .. Endothelial cells were also fixed and stained in 0.5 μg of anti-TLR4 as above, but the signal was amplified with donkey anti-rabbit biotin conjugate secondary (Santa Cruz Biotechnology, Santa Cruz, CA) followed by a streptavidin PE conjugate (Caltag Laboratories, Burlingame, CA).

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  • 85
    Jackson Immuno biotin sp conjugated affinipuref
    Biotin Sp Conjugated Affinipuref, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp conjugated affinipuref/product/Jackson Immuno
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipuref - by Bioz Stars, 2020-05
    85/100 stars
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    94
    Jackson Immuno biotin sp affinipure donkey anti mouse igg
    Biotin Sp Affinipure Donkey Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure donkey anti mouse igg/product/Jackson Immuno
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure donkey anti mouse igg - by Bioz Stars, 2020-05
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    92
    Jackson Immuno biotin sp affinipure donkey anti rabbit igg
    Biotin Sp Affinipure Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure donkey anti rabbit igg/product/Jackson Immuno
    Average 92 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure donkey anti rabbit igg - by Bioz Stars, 2020-05
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    85
    Jackson Immuno biotinylated sp conjugated affinpure donkey anti rabbit igg
    Biotinylated Sp Conjugated Affinpure Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated sp conjugated affinpure donkey anti rabbit igg/product/Jackson Immuno
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated sp conjugated affinpure donkey anti rabbit igg - by Bioz Stars, 2020-05
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