biotin sp affinipure donkey anti goat igg  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Donkey Anti Goat IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    705-065-003
    Price:
    98
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Donkey
    Quantity:
    1 0
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure donkey anti goat igg
    Biotin SP AffiniPure Donkey Anti Goat IgG
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure donkey anti goat igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure donkey anti goat igg - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Immunocytochemistry:

    Article Title: Dopamine D1 Receptor Immunoreactivity on Fine Processes of GFAP-Positive Astrocytes in the Substantia Nigra Pars Reticulata of Adult Mouse
    Article Snippet: Secondary antibodies used for the avidin-biotin-peroxidase complex method were biotinylated donkey anti-rabbit antibody (711-065-152; 1:1000, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for parvalbumin, donkey anti-mouse antibody (715-065-151; 1:1000, Jackson ImmunoResearch Laboratories) for TH, donkey anti-goat antibody (705-065-147; 1:1000, Jackson ImmunoResearch Laboratories) for D1R, and biotinylated donkey anti-guinea pig antibody (706-065-148; 1:1000, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for D2R. .. Secondary antibodies used for immunocytochemistry of acutely dissociated cells were Alexa Fluor 488 donkey anti-goat IgG (A-11055; 1:1000, Life Technologies, Waltham, MA, USA) and Cy3-conjugate and donkey anti-rabbit IgG (711-165-152; 1:400, Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

    Stable Transfection:

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: For immunofluorescence staining, Hepa1-6 cells stably expressing RORα and skin cells were seeded in chamber slides (Thermo Scientific) and processed as described previously ( ). .. The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature.

    Blocking Assay:

    Article Title: Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin-CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
    Article Snippet: Mounted sections were processed for antigen retrieval (0.01 M citric acid, pH 6.0, 95°C, 15 min), and nonspecific staining was blocked by incubation in blocking solution (3% normal donkey serum [NDS] vol/vol in 0.1% Triton X-100 in 1X PBS) for 30 min. .. The following day, sections were rinsed and incubated in biotinylated-donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, Cat. #711-065-162) or donkey anti-goat IgG antibody (1:200; Jackson ImmunoResearch, Cat. #705-065-003) in 1.5% NDS in 1XPBS for 1 hr.

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: After being extensively rinsed in PBS, cells were incubated overnight at 4°C with goat anti-RORα antibody (sc-6062; Santa Cruz Biotechnology), diluted 1:200 in blocking solution. .. The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature.

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: For all markers, non-specific binding was blocked with 3% serum (donkey) and 0.3% Triton-X in PBS for 1 h. Following pretreatment and blocking, the slide-mounted sections were incubated with either rabbit-α-Ki67 (1:500; Fisher Scientific, catalog #RM-9106S, Freemont, CA, USA), rat-α-BrdU (1:500; Accurate, catalog #OBT 0030G, Westbury, NY, USA), or goat-α-DCX (1:8000; Santa Cruz Biotechnology, catalog #sc-8066, Dallas, TX, USA) in 3% serum and 0.3% Tween-20 overnight. .. After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100).

    Article Title: Area-specific analysis of the distribution of hypothalamic neurons projecting to the rat ventral tegmental area, with special reference to the GABAergic and glutamatergic efferents
    Article Snippet: The floating sections underwent a series of pre-treatment steps, including permeabilization with Triton X-100 (0.5% in PBS, 20 min), blocking of endogenous peroxidase activity with H2 O2 (0.5% in PBS, 20 min) and preventing non-specific antibody binding to the tissue with normal horse serum (2% in PBS, 10 min). .. The pre-treatment was followed by sequential incubations in goat anti-CTB serum (1:1,000; 24 h; RT; #703; List Biological Laboratories, INC, Campbell, CA, USA), in biotinylated donkey anti-goat IgG (1:500; 2 h; 705-065-147, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and in avidin–biotin–peroxidase complex (ABC; 1:1,000; 2 h; PK-6100, Vector Laboratories).

    Incubation:

    Article Title: Nitric oxide deficiency and endothelial–mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice
    Article Snippet: .. To visualize expression of Snail, the slices were incubated with primary anti-Snail antibody (ab53519; Abcam) and secondary biotinylated donkey anti-goat antibodies (705-065-147; Jackson ImmunoResearch) concomitantly with ABC vector complex. .. For each slice, 10 randomly chosen nonobstructed arteries were photographed with a BX51 microscope (objective 20×) equipped with the virtual microscopy system dotSlide (Olympus, Japan) and the length of Snail-positive fragment(s) within the artery was manually measured and expressed as the percentage of the entire circuit of the particular artery.

    Article Title: Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin-CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
    Article Snippet: .. The following day, sections were rinsed and incubated in biotinylated-donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, Cat. #711-065-162) or donkey anti-goat IgG antibody (1:200; Jackson ImmunoResearch, Cat. #705-065-003) in 1.5% NDS in 1XPBS for 1 hr. .. After rinses and 30 min incubation in 0.3% hydrogen peroxide in 1XPBS, and sections were incubated in avidin-biotin complex (Vector Laboratories) for 60 min. After rinsing, staining was visualized using DAB/metal concentration (Thermo Scientific, Cat. #1856090) or fluorescein-labeled tyramide signal amplication (PerkinElmer, Cat. #SAT701).

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: .. The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature. .. Immunocomplexes were evaluated and photographed with a fluorescent microscope conjugated digital camera (Leica Digital DM4000B; Leica Microsystems, Oberkochen, Germany).

    Article Title: Glutamatergic Preoptic Area Neurons That Express Leptin Receptors Drive Temperature-Dependent Body Weight Homeostasis
    Article Snippet: Brains were sliced at 30 μm thickness and processed for free-floating immunohistochemistry. pSTAT3 and occasionally cFos were visualized by diaminobenzene (DAB; #34065, Thermo Fisher Scientific) following treatment with Vectastain ABC (#PK-6100, Vector Laboratories) after incubation with biotinylated secondary antibodies. .. Secondary antibodies used were donkey anti-goat IgG-Alexa594 (# , Life Technologies), donkey anti-goat IgG-Alexa488 (# , Life Technologies), donkey anti-rabbit IgG-Alexa594 (# , Life Technologies), donkey anti-chicken IgY-DyLight488 (#703-486-155, Jackson ImmunoResearch Laboratories), donkey anti-goat IgG-biotin (#705-065-003, Jackson ImmunoResearch Laboratories), and donkey anti-rabbit IgG-biotin (#711-065-152, Jackson ImmunoResearch Laboratories).

    Article Title: Calbindin content and differential vulnerability of midbrain efferent dopaminergic neurons in macaques
    Article Snippet: .. After rinsing in PBS, sections were incubated for 2 h in a solution containing 5% of NSwS, 5% NDS, swine anti-rabbit IgG (1:50, Dako, Z0196) and donkey anti-goat (Jackson Immunoresearch, 705-065-147) diluted in PBS for 90 min; after washes sections were incubated in a solution containing 5% NDS and Goat PAP (1:600 Sigma, P1901) and afterwards washed in PBS and visualized in brown with DAB (Sigma, D5637). .. Section were washed in PBS and incubated in a solution containing 5% NSwS and Rabbit PAP (1:50 Dako, Z0113 diluted in PBS and visualized using Vector VIP Peroxidase (HRP) Substrate Kit (Vector laboratories, SK-4600).

    Article Title: SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes
    Article Snippet: The slides were cooled down, washed three times in PBS-T, and subsequently blocked with 5% FCS in PBS-T over 20 min. Next, tissue sections were incubated overnight at 4°C with the first polyclonal antibody, anti-nectin-4 (AF2659; R & D Systems) at a concentration of 1:1,000 in PBS-T under a humid atmosphere. .. Successively, biotinylated donkey anti-goat secondary antibody (1:1,000 in PBS-T) (705-065-003; Jackson ImmunoResearch) and peroxidase-labeled streptavidin conjugate (1:1,000 in PBS-T) (016-030-084; Jackson ImmunoResearch) were added for 30 min each at room temperature in the dark.

    Article Title: Neuronal and Nonneuronal Cholinergic Structures in the Mouse Gastrointestinal Tract and Spleen
    Article Snippet: .. After several washes, sections were incubated in anti-rabbit Alexa Fluor 488- (Invitrogen, Carlsbad, CA, cat. ; 1:1,000) or anti-rat Alexa Fluor 488- (Invitrogen, cat. ; 1:1,000) or biotinylated anti-goat (Jackson ImmunoResearch, West Grove, PA, cat. 705-065-147; 1:1,000) secondary antibody for 1 hour at room temperature. .. Following the biotinylated secondary antibodies, tissue was incubated with streptavidin Alexa Fluor 488 (Invitrogen, cat. ; 1:1,000).

    Article Title: Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: Involvement of 5-HT7 receptors
    Article Snippet: .. After three washes, sections were incubated with biotin conjugated donkey anti-goat (1:400; Jackson ImmunoResearch, cat. 705-065-003, West Grove, PA, USA) or goat anti-rabbit (1:250; Vector Laboratories, cat. BA-1000, Burlingame, CA, USA) secondary antibodies for 2 h at room temperature. .. After several washes in 0.1M PBS, sections were incubated with the avidine-biotin-peroxidase complex (VECTASTAIN Elite ABC kit, 1:200; Vector Laboratories, Burlingame, CA, USA) in 0.1M PBS with 0.03% triton X-100 for 2 h at room temperature.

    Article Title: Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus
    Article Snippet: .. The specimens were then washed in three changes of PBS and incubated with biotin-conjugated goat anti-rabbit IgG solution (1:400, BA1000, Vector) (for NOS) or donkey anti-goat IgG solution (1:400, 705-065-147, Jackson ImmunoResearch Laboratories) (for ChAT) for 1 h, followed by incubation with avidin-biotin peroxidase complex (Elite ABCkit; Vector) for l h at room temperature. ..

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: .. After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100). .. Immuno-labeled cells were visualized with metal-enhanced diaminobenzidine (DAB, Fisher Scientific, catalog #PI-34065, Pittsburgh, PA, USA) for 5–10 min.

    Activity Assay:

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: Endogenous peroxidase activity was inhibited by incubating with 0.3% hydrogen peroxide (H2 O2 ) for 30 min. For BrdU IHC, two additional pretreatment steps were performed to allow the antibody access to DNA inside the cell nucleus: permeabilization and denaturation. .. After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100).

    Article Title: Area-specific analysis of the distribution of hypothalamic neurons projecting to the rat ventral tegmental area, with special reference to the GABAergic and glutamatergic efferents
    Article Snippet: The floating sections underwent a series of pre-treatment steps, including permeabilization with Triton X-100 (0.5% in PBS, 20 min), blocking of endogenous peroxidase activity with H2 O2 (0.5% in PBS, 20 min) and preventing non-specific antibody binding to the tissue with normal horse serum (2% in PBS, 10 min). .. The pre-treatment was followed by sequential incubations in goat anti-CTB serum (1:1,000; 24 h; RT; #703; List Biological Laboratories, INC, Campbell, CA, USA), in biotinylated donkey anti-goat IgG (1:500; 2 h; 705-065-147, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and in avidin–biotin–peroxidase complex (ABC; 1:1,000; 2 h; PK-6100, Vector Laboratories).

    Expressing:

    Article Title: Nitric oxide deficiency and endothelial–mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice
    Article Snippet: .. To visualize expression of Snail, the slices were incubated with primary anti-Snail antibody (ab53519; Abcam) and secondary biotinylated donkey anti-goat antibodies (705-065-147; Jackson ImmunoResearch) concomitantly with ABC vector complex. .. For each slice, 10 randomly chosen nonobstructed arteries were photographed with a BX51 microscope (objective 20×) equipped with the virtual microscopy system dotSlide (Olympus, Japan) and the length of Snail-positive fragment(s) within the artery was manually measured and expressed as the percentage of the entire circuit of the particular artery.

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: For immunofluorescence staining, Hepa1-6 cells stably expressing RORα and skin cells were seeded in chamber slides (Thermo Scientific) and processed as described previously ( ). .. The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature.

    Electron Microscopy:

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100). .. They were then dehydrated in increasing ethanol concentrations and Citrisolv, and cover slipped with DPX (Electron Microscopy Sciences, catalog #13512).

    Immunohistochemistry:

    Article Title: Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin-CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
    Article Snippet: Paragraph title: Immunohistochemistry (IHC) ... The following day, sections were rinsed and incubated in biotinylated-donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, Cat. #711-065-162) or donkey anti-goat IgG antibody (1:200; Jackson ImmunoResearch, Cat. #705-065-003) in 1.5% NDS in 1XPBS for 1 hr.

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: Paragraph title: Immunohistochemistry/immunofluorescence studies ... The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature.

    Article Title: Glutamatergic Preoptic Area Neurons That Express Leptin Receptors Drive Temperature-Dependent Body Weight Homeostasis
    Article Snippet: Paragraph title: Perfusion and immunohistochemistry. ... Secondary antibodies used were donkey anti-goat IgG-Alexa594 (# , Life Technologies), donkey anti-goat IgG-Alexa488 (# , Life Technologies), donkey anti-rabbit IgG-Alexa594 (# , Life Technologies), donkey anti-chicken IgY-DyLight488 (#703-486-155, Jackson ImmunoResearch Laboratories), donkey anti-goat IgG-biotin (#705-065-003, Jackson ImmunoResearch Laboratories), and donkey anti-rabbit IgG-biotin (#711-065-152, Jackson ImmunoResearch Laboratories).

    Article Title: Calbindin content and differential vulnerability of midbrain efferent dopaminergic neurons in macaques
    Article Snippet: Detection of transported CTB and fluorogold Immunohistochemical detection of transported CTB and FG was carried out on coronal sections. .. After rinsing in PBS, sections were incubated for 2 h in a solution containing 5% of NSwS, 5% NDS, swine anti-rabbit IgG (1:50, Dako, Z0196) and donkey anti-goat (Jackson Immunoresearch, 705-065-147) diluted in PBS for 90 min; after washes sections were incubated in a solution containing 5% NDS and Goat PAP (1:600 Sigma, P1901) and afterwards washed in PBS and visualized in brown with DAB (Sigma, D5637).

    Article Title: SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes
    Article Snippet: Paragraph title: Immunohistochemistry. ... Successively, biotinylated donkey anti-goat secondary antibody (1:1,000 in PBS-T) (705-065-003; Jackson ImmunoResearch) and peroxidase-labeled streptavidin conjugate (1:1,000 in PBS-T) (016-030-084; Jackson ImmunoResearch) were added for 30 min each at room temperature in the dark.

    Article Title: Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: Involvement of 5-HT7 receptors
    Article Snippet: Paragraph title: 2.6. Immunohistochemistry assays ... After three washes, sections were incubated with biotin conjugated donkey anti-goat (1:400; Jackson ImmunoResearch, cat. 705-065-003, West Grove, PA, USA) or goat anti-rabbit (1:250; Vector Laboratories, cat. BA-1000, Burlingame, CA, USA) secondary antibodies for 2 h at room temperature.

    Article Title: Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus
    Article Snippet: Paragraph title: Immunohistochemistry ... The specimens were then washed in three changes of PBS and incubated with biotin-conjugated goat anti-rabbit IgG solution (1:400, BA1000, Vector) (for NOS) or donkey anti-goat IgG solution (1:400, 705-065-147, Jackson ImmunoResearch Laboratories) (for ChAT) for 1 h, followed by incubation with avidin-biotin peroxidase complex (Elite ABCkit; Vector) for l h at room temperature.

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: Paragraph title: 4.3. Tissue Preparation and Immunohistochemistry (IHC) ... After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100).

    Article Title: Area-specific analysis of the distribution of hypothalamic neurons projecting to the rat ventral tegmental area, with special reference to the GABAergic and glutamatergic efferents
    Article Snippet: Paragraph title: Single-Label Immunohistochemistry to Detect CTB ... The pre-treatment was followed by sequential incubations in goat anti-CTB serum (1:1,000; 24 h; RT; #703; List Biological Laboratories, INC, Campbell, CA, USA), in biotinylated donkey anti-goat IgG (1:500; 2 h; 705-065-147, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and in avidin–biotin–peroxidase complex (ABC; 1:1,000; 2 h; PK-6100, Vector Laboratories).

    Dissection:

    Article Title: Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus
    Article Snippet: It was washed out in three changes of PBS (pH 7.3), then the mucosa and the outer and inner muscle layers were removed with fine forceps under a dissection microscope for whole-mount preparation. .. The specimens were then washed in three changes of PBS and incubated with biotin-conjugated goat anti-rabbit IgG solution (1:400, BA1000, Vector) (for NOS) or donkey anti-goat IgG solution (1:400, 705-065-147, Jackson ImmunoResearch Laboratories) (for ChAT) for 1 h, followed by incubation with avidin-biotin peroxidase complex (Elite ABCkit; Vector) for l h at room temperature.

    Injection:

    Article Title: Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus
    Article Snippet: After removal of the oesophagus, one end of the oesophagus was ligated with a silk thread, and the lumen was distended with a fixative solution of 4 % paraformaldehyde in 0.1 M phosphate buffer (PB) injected through a needle inserted into the other end of the oesophagus. .. The specimens were then washed in three changes of PBS and incubated with biotin-conjugated goat anti-rabbit IgG solution (1:400, BA1000, Vector) (for NOS) or donkey anti-goat IgG solution (1:400, 705-065-147, Jackson ImmunoResearch Laboratories) (for ChAT) for 1 h, followed by incubation with avidin-biotin peroxidase complex (Elite ABCkit; Vector) for l h at room temperature.

    Article Title: Area-specific analysis of the distribution of hypothalamic neurons projecting to the rat ventral tegmental area, with special reference to the GABAergic and glutamatergic efferents
    Article Snippet: Single-Label Immunohistochemistry to Detect CTB One out of the six groups of sections from each brain was processed for plotting the injection sites and mapping the distribution of the labeled cells. .. The pre-treatment was followed by sequential incubations in goat anti-CTB serum (1:1,000; 24 h; RT; #703; List Biological Laboratories, INC, Campbell, CA, USA), in biotinylated donkey anti-goat IgG (1:500; 2 h; 705-065-147, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and in avidin–biotin–peroxidase complex (ABC; 1:1,000; 2 h; PK-6100, Vector Laboratories).

    Binding Assay:

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: For all markers, non-specific binding was blocked with 3% serum (donkey) and 0.3% Triton-X in PBS for 1 h. Following pretreatment and blocking, the slide-mounted sections were incubated with either rabbit-α-Ki67 (1:500; Fisher Scientific, catalog #RM-9106S, Freemont, CA, USA), rat-α-BrdU (1:500; Accurate, catalog #OBT 0030G, Westbury, NY, USA), or goat-α-DCX (1:8000; Santa Cruz Biotechnology, catalog #sc-8066, Dallas, TX, USA) in 3% serum and 0.3% Tween-20 overnight. .. After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100).

    Article Title: Area-specific analysis of the distribution of hypothalamic neurons projecting to the rat ventral tegmental area, with special reference to the GABAergic and glutamatergic efferents
    Article Snippet: The floating sections underwent a series of pre-treatment steps, including permeabilization with Triton X-100 (0.5% in PBS, 20 min), blocking of endogenous peroxidase activity with H2 O2 (0.5% in PBS, 20 min) and preventing non-specific antibody binding to the tissue with normal horse serum (2% in PBS, 10 min). .. The pre-treatment was followed by sequential incubations in goat anti-CTB serum (1:1,000; 24 h; RT; #703; List Biological Laboratories, INC, Campbell, CA, USA), in biotinylated donkey anti-goat IgG (1:500; 2 h; 705-065-147, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and in avidin–biotin–peroxidase complex (ABC; 1:1,000; 2 h; PK-6100, Vector Laboratories).

    Immunofluorescence:

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: For immunofluorescence staining, Hepa1-6 cells stably expressing RORα and skin cells were seeded in chamber slides (Thermo Scientific) and processed as described previously ( ). .. The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature.

    Fluorescence:

    Article Title: Neuronal and Nonneuronal Cholinergic Structures in the Mouse Gastrointestinal Tract and Spleen
    Article Snippet: TdTomato protein native fluorescence was directly visualized in whole mounts or in cryostatcut sections (16 μm) collected on SuperFrost slides after an overnight incubation in 20% sucrose. .. After several washes, sections were incubated in anti-rabbit Alexa Fluor 488- (Invitrogen, Carlsbad, CA, cat. ; 1:1,000) or anti-rat Alexa Fluor 488- (Invitrogen, cat. ; 1:1,000) or biotinylated anti-goat (Jackson ImmunoResearch, West Grove, PA, cat. 705-065-147; 1:1,000) secondary antibody for 1 hour at room temperature.

    CtB Assay:

    Article Title: Calbindin content and differential vulnerability of midbrain efferent dopaminergic neurons in macaques
    Article Snippet: Paragraph title: Detection of transported CTB and fluorogold ... After rinsing in PBS, sections were incubated for 2 h in a solution containing 5% of NSwS, 5% NDS, swine anti-rabbit IgG (1:50, Dako, Z0196) and donkey anti-goat (Jackson Immunoresearch, 705-065-147) diluted in PBS for 90 min; after washes sections were incubated in a solution containing 5% NDS and Goat PAP (1:600 Sigma, P1901) and afterwards washed in PBS and visualized in brown with DAB (Sigma, D5637).

    Article Title: Area-specific analysis of the distribution of hypothalamic neurons projecting to the rat ventral tegmental area, with special reference to the GABAergic and glutamatergic efferents
    Article Snippet: Paragraph title: Single-Label Immunohistochemistry to Detect CTB ... The pre-treatment was followed by sequential incubations in goat anti-CTB serum (1:1,000; 24 h; RT; #703; List Biological Laboratories, INC, Campbell, CA, USA), in biotinylated donkey anti-goat IgG (1:500; 2 h; 705-065-147, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and in avidin–biotin–peroxidase complex (ABC; 1:1,000; 2 h; PK-6100, Vector Laboratories).

    Avidin-Biotin Assay:

    Article Title: Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin-CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
    Article Snippet: The following day, sections were rinsed and incubated in biotinylated-donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, Cat. #711-065-162) or donkey anti-goat IgG antibody (1:200; Jackson ImmunoResearch, Cat. #705-065-003) in 1.5% NDS in 1XPBS for 1 hr. .. After rinses and 30 min incubation in 0.3% hydrogen peroxide in 1XPBS, and sections were incubated in avidin-biotin complex (Vector Laboratories) for 60 min. After rinsing, staining was visualized using DAB/metal concentration (Thermo Scientific, Cat. #1856090) or fluorescein-labeled tyramide signal amplication (PerkinElmer, Cat. #SAT701).

    Article Title: Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus
    Article Snippet: .. The specimens were then washed in three changes of PBS and incubated with biotin-conjugated goat anti-rabbit IgG solution (1:400, BA1000, Vector) (for NOS) or donkey anti-goat IgG solution (1:400, 705-065-147, Jackson ImmunoResearch Laboratories) (for ChAT) for 1 h, followed by incubation with avidin-biotin peroxidase complex (Elite ABCkit; Vector) for l h at room temperature. ..

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: .. After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100). .. Immuno-labeled cells were visualized with metal-enhanced diaminobenzidine (DAB, Fisher Scientific, catalog #PI-34065, Pittsburgh, PA, USA) for 5–10 min.

    Article Title: Dopamine D1 Receptor Immunoreactivity on Fine Processes of GFAP-Positive Astrocytes in the Substantia Nigra Pars Reticulata of Adult Mouse
    Article Snippet: .. Secondary antibodies used for the avidin-biotin-peroxidase complex method were biotinylated donkey anti-rabbit antibody (711-065-152; 1:1000, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for parvalbumin, donkey anti-mouse antibody (715-065-151; 1:1000, Jackson ImmunoResearch Laboratories) for TH, donkey anti-goat antibody (705-065-147; 1:1000, Jackson ImmunoResearch Laboratories) for D1R, and biotinylated donkey anti-guinea pig antibody (706-065-148; 1:1000, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for D2R. .. Secondary antibodies used for immunocytochemistry of acutely dissociated cells were Alexa Fluor 488 donkey anti-goat IgG (A-11055; 1:1000, Life Technologies, Waltham, MA, USA) and Cy3-conjugate and donkey anti-rabbit IgG (711-165-152; 1:400, Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

    Article Title: Area-specific analysis of the distribution of hypothalamic neurons projecting to the rat ventral tegmental area, with special reference to the GABAergic and glutamatergic efferents
    Article Snippet: .. The pre-treatment was followed by sequential incubations in goat anti-CTB serum (1:1,000; 24 h; RT; #703; List Biological Laboratories, INC, Campbell, CA, USA), in biotinylated donkey anti-goat IgG (1:500; 2 h; 705-065-147, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and in avidin–biotin–peroxidase complex (ABC; 1:1,000; 2 h; PK-6100, Vector Laboratories). ..

    Labeling:

    Article Title: Area-specific analysis of the distribution of hypothalamic neurons projecting to the rat ventral tegmental area, with special reference to the GABAergic and glutamatergic efferents
    Article Snippet: Single-Label Immunohistochemistry to Detect CTB One out of the six groups of sections from each brain was processed for plotting the injection sites and mapping the distribution of the labeled cells. .. The pre-treatment was followed by sequential incubations in goat anti-CTB serum (1:1,000; 24 h; RT; #703; List Biological Laboratories, INC, Campbell, CA, USA), in biotinylated donkey anti-goat IgG (1:500; 2 h; 705-065-147, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and in avidin–biotin–peroxidase complex (ABC; 1:1,000; 2 h; PK-6100, Vector Laboratories).

    Mouse Assay:

    Article Title: Neuronal and Nonneuronal Cholinergic Structures in the Mouse Gastrointestinal Tract and Spleen
    Article Snippet: On the day of sacrifice, between 9 and 11A.M., mice were deeply anesthetized with chloral hydrate (500 mg/kg, i.p.) and transcardially perfused with 10% formalin. .. After several washes, sections were incubated in anti-rabbit Alexa Fluor 488- (Invitrogen, Carlsbad, CA, cat. ; 1:1,000) or anti-rat Alexa Fluor 488- (Invitrogen, cat. ; 1:1,000) or biotinylated anti-goat (Jackson ImmunoResearch, West Grove, PA, cat. 705-065-147; 1:1,000) secondary antibody for 1 hour at room temperature.

    Microscopy:

    Article Title: Nitric oxide deficiency and endothelial–mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice
    Article Snippet: To visualize expression of Snail, the slices were incubated with primary anti-Snail antibody (ab53519; Abcam) and secondary biotinylated donkey anti-goat antibodies (705-065-147; Jackson ImmunoResearch) concomitantly with ABC vector complex. .. For each slice, 10 randomly chosen nonobstructed arteries were photographed with a BX51 microscope (objective 20×) equipped with the virtual microscopy system dotSlide (Olympus, Japan) and the length of Snail-positive fragment(s) within the artery was manually measured and expressed as the percentage of the entire circuit of the particular artery.

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature. .. Immunocomplexes were evaluated and photographed with a fluorescent microscope conjugated digital camera (Leica Digital DM4000B; Leica Microsystems, Oberkochen, Germany).

    Article Title: Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus
    Article Snippet: It was washed out in three changes of PBS (pH 7.3), then the mucosa and the outer and inner muscle layers were removed with fine forceps under a dissection microscope for whole-mount preparation. .. The specimens were then washed in three changes of PBS and incubated with biotin-conjugated goat anti-rabbit IgG solution (1:400, BA1000, Vector) (for NOS) or donkey anti-goat IgG solution (1:400, 705-065-147, Jackson ImmunoResearch Laboratories) (for ChAT) for 1 h, followed by incubation with avidin-biotin peroxidase complex (Elite ABCkit; Vector) for l h at room temperature.

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: One series of the hippocampus (every ninth section) was mounted onto Fisher Scientific Microscope Superfrost/Plus Precleaned charged slides from anterior to posterior and allowed to dry for 2 h. Antigen retrieval was performed using 0.01 M citric acid (pH 6.0) at 100 °C for 15 min, then was washed in PBS at room temperature. .. After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100).

    Purification:

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: The RORγ antibodies were purified with a Melon Gel IgG Purification Kit (Thermo Scientific, Lafayette, CO, USA), followed by incubation with MBP-bound amylose resin for 4 h to remove MBP-specific antibodies. .. The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature.

    Plasmid Preparation:

    Article Title: Nitric oxide deficiency and endothelial–mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice
    Article Snippet: .. To visualize expression of Snail, the slices were incubated with primary anti-Snail antibody (ab53519; Abcam) and secondary biotinylated donkey anti-goat antibodies (705-065-147; Jackson ImmunoResearch) concomitantly with ABC vector complex. .. For each slice, 10 randomly chosen nonobstructed arteries were photographed with a BX51 microscope (objective 20×) equipped with the virtual microscopy system dotSlide (Olympus, Japan) and the length of Snail-positive fragment(s) within the artery was manually measured and expressed as the percentage of the entire circuit of the particular artery.

    Article Title: Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin-CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
    Article Snippet: The following day, sections were rinsed and incubated in biotinylated-donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, Cat. #711-065-162) or donkey anti-goat IgG antibody (1:200; Jackson ImmunoResearch, Cat. #705-065-003) in 1.5% NDS in 1XPBS for 1 hr. .. The following day, sections were rinsed and incubated in biotinylated-donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, Cat. #711-065-162) or donkey anti-goat IgG antibody (1:200; Jackson ImmunoResearch, Cat. #705-065-003) in 1.5% NDS in 1XPBS for 1 hr.

    Article Title: Calbindin content and differential vulnerability of midbrain efferent dopaminergic neurons in macaques
    Article Snippet: After rinsing in PBS, sections were incubated for 2 h in a solution containing 5% of NSwS, 5% NDS, swine anti-rabbit IgG (1:50, Dako, Z0196) and donkey anti-goat (Jackson Immunoresearch, 705-065-147) diluted in PBS for 90 min; after washes sections were incubated in a solution containing 5% NDS and Goat PAP (1:600 Sigma, P1901) and afterwards washed in PBS and visualized in brown with DAB (Sigma, D5637). .. Section were washed in PBS and incubated in a solution containing 5% NSwS and Rabbit PAP (1:50 Dako, Z0113 diluted in PBS and visualized using Vector VIP Peroxidase (HRP) Substrate Kit (Vector laboratories, SK-4600).

    Article Title: Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: Involvement of 5-HT7 receptors
    Article Snippet: After three washes, sections were incubated with biotin conjugated donkey anti-goat (1:400; Jackson ImmunoResearch, cat. 705-065-003, West Grove, PA, USA) or goat anti-rabbit (1:250; Vector Laboratories, cat. BA-1000, Burlingame, CA, USA) secondary antibodies for 2 h at room temperature. .. After three washes, sections were incubated with biotin conjugated donkey anti-goat (1:400; Jackson ImmunoResearch, cat. 705-065-003, West Grove, PA, USA) or goat anti-rabbit (1:250; Vector Laboratories, cat. BA-1000, Burlingame, CA, USA) secondary antibodies for 2 h at room temperature.

    Article Title: Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus
    Article Snippet: .. The specimens were then washed in three changes of PBS and incubated with biotin-conjugated goat anti-rabbit IgG solution (1:400, BA1000, Vector) (for NOS) or donkey anti-goat IgG solution (1:400, 705-065-147, Jackson ImmunoResearch Laboratories) (for ChAT) for 1 h, followed by incubation with avidin-biotin peroxidase complex (Elite ABCkit; Vector) for l h at room temperature. ..

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100). .. Finally, slides were incubated for four-min in the nuclear counterstain Fast Red (Vector Laboratories catalog #H3403).

    Double Immunostaining:

    Article Title: Role of intrinsic nitrergic neurones on vagally mediated striated muscle contractions in the hamster oesophagus
    Article Snippet: The specimens were then washed in three changes of PBS and incubated with biotin-conjugated goat anti-rabbit IgG solution (1:400, BA1000, Vector) (for NOS) or donkey anti-goat IgG solution (1:400, 705-065-147, Jackson ImmunoResearch Laboratories) (for ChAT) for 1 h, followed by incubation with avidin-biotin peroxidase complex (Elite ABCkit; Vector) for l h at room temperature. .. For double immunostaining of NOS and vanilloid receptor 1 (VR1), whole-mount specimens, after 1 h exposure to 1 % normal donkey serum, were incubated with a mixture of the rabbit anti-NOS serum (1:1000, 24287, Diasorin) and goat anti-VR1 serum (1:200, SC-8670, Santa Cruz) for 6 days at 4 °C.

    Concentration Assay:

    Article Title: Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin-CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
    Article Snippet: The following day, sections were rinsed and incubated in biotinylated-donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, Cat. #711-065-162) or donkey anti-goat IgG antibody (1:200; Jackson ImmunoResearch, Cat. #705-065-003) in 1.5% NDS in 1XPBS for 1 hr. .. After rinses and 30 min incubation in 0.3% hydrogen peroxide in 1XPBS, and sections were incubated in avidin-biotin complex (Vector Laboratories) for 60 min. After rinsing, staining was visualized using DAB/metal concentration (Thermo Scientific, Cat. #1856090) or fluorescein-labeled tyramide signal amplication (PerkinElmer, Cat. #SAT701).

    Article Title: SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes
    Article Snippet: The slides were cooled down, washed three times in PBS-T, and subsequently blocked with 5% FCS in PBS-T over 20 min. Next, tissue sections were incubated overnight at 4°C with the first polyclonal antibody, anti-nectin-4 (AF2659; R & D Systems) at a concentration of 1:1,000 in PBS-T under a humid atmosphere. .. Successively, biotinylated donkey anti-goat secondary antibody (1:1,000 in PBS-T) (705-065-003; Jackson ImmunoResearch) and peroxidase-labeled streptavidin conjugate (1:1,000 in PBS-T) (016-030-084; Jackson ImmunoResearch) were added for 30 min each at room temperature in the dark.

    Staining:

    Article Title: Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin-CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
    Article Snippet: Mounted sections were processed for antigen retrieval (0.01 M citric acid, pH 6.0, 95°C, 15 min), and nonspecific staining was blocked by incubation in blocking solution (3% normal donkey serum [NDS] vol/vol in 0.1% Triton X-100 in 1X PBS) for 30 min. .. The following day, sections were rinsed and incubated in biotinylated-donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, Cat. #711-065-162) or donkey anti-goat IgG antibody (1:200; Jackson ImmunoResearch, Cat. #705-065-003) in 1.5% NDS in 1XPBS for 1 hr.

    Article Title: RORα and ROR γ are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D
    Article Snippet: For immunofluorescence staining, Hepa1-6 cells stably expressing RORα and skin cells were seeded in chamber slides (Thermo Scientific) and processed as described previously ( ). .. The next day, after being extensively rinsed, cells were incubated with biotinylated donkey anti-goat (705-065-147) IgG (1:1000) at room temperature for 1 h and next the fluorophore red CY3-streptavidine (016-160-084; Jackson Immunoresearch, West Grove, PA, USA) was applied for 1 h at room temperature.

    Article Title: Whole-Body 12C Irradiation Transiently Decreases Mouse Hippocampal Dentate Gyrus Proliferation and Immature Neuron Number, but Does Not Change New Neuron Survival Rate
    Article Snippet: To stain for neurogenesis relevant markers ( B) [ ], slide-mounted IHC for DCX+, Ki67+, and BrdU+ cells in the DG was performed, as previously described [ , , , ]. .. After primary antibody, incubation with biotinylated secondary antibodies for DCX: biotin-donkey-α-goat-IgG (catalog #705-065-003), for Ki67: biotin-donkey-α-rabbit-IgG (catalog #711-065-152), or for BrdU: biotin-donkey-α-rat-IgG (catalog #712-065-153), all 1:200, all from Jackson ImmunoResearch, West Grove, PA, USA) for 1 h. Incubation with avidin-biotin complex was performed for 90 min (ABC Elite, Vector Laboratories, catalog #PK-6100).

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