biotin conjugated goat anti rabbit igg  (Thermo Fisher)


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    Structured Review

    Thermo Fisher biotin conjugated goat anti rabbit igg
    Proximal tubule cells secrete <t>pro-AOAH.</t> (A) Diagram of AOAH structure, showing the conversion of the precursor (pro-AOAH) into mature AOAH. (B) Production of 35 S-AOAH by porcine proximal tubule cells in vitro. Labeled AOAH was immunoprecipitated as described in Methods and studied by SDS-PAGE and autoradiography. M, medium; L, lysate. Antibody +, anti-AOAH; antibody −, preimmune <t>IgG.</t> Only pro-AOAH is seen in the media, whereas the lysate contains both pro-AOAH and mature AOAH.
    Biotin Conjugated Goat Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat anti rabbit igg/product/Thermo Fisher
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit igg - by Bioz Stars, 2020-09
    92/100 stars

    Images

    1) Product Images from "Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract "

    Article Title: Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract

    Journal: Infection and Immunity

    doi: 10.1128/IAI.72.6.3171-3178.2004

    Proximal tubule cells secrete pro-AOAH. (A) Diagram of AOAH structure, showing the conversion of the precursor (pro-AOAH) into mature AOAH. (B) Production of 35 S-AOAH by porcine proximal tubule cells in vitro. Labeled AOAH was immunoprecipitated as described in Methods and studied by SDS-PAGE and autoradiography. M, medium; L, lysate. Antibody +, anti-AOAH; antibody −, preimmune IgG. Only pro-AOAH is seen in the media, whereas the lysate contains both pro-AOAH and mature AOAH.
    Figure Legend Snippet: Proximal tubule cells secrete pro-AOAH. (A) Diagram of AOAH structure, showing the conversion of the precursor (pro-AOAH) into mature AOAH. (B) Production of 35 S-AOAH by porcine proximal tubule cells in vitro. Labeled AOAH was immunoprecipitated as described in Methods and studied by SDS-PAGE and autoradiography. M, medium; L, lysate. Antibody +, anti-AOAH; antibody −, preimmune IgG. Only pro-AOAH is seen in the media, whereas the lysate contains both pro-AOAH and mature AOAH.

    Techniques Used: In Vitro, Labeling, Immunoprecipitation, SDS Page, Autoradiography

    2) Product Images from "Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract"

    Article Title: Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract

    Journal: Infection and Immunity

    doi: 10.1128/IAI.72.6.3171-3178.2004

    Proximal tubule cells secrete pro-AOAH. (A) Diagram of AOAH structure, showing the conversion of the precursor (pro-AOAH) into mature AOAH. (B) Production of 35 S-AOAH by porcine proximal tubule cells in vitro. Labeled AOAH was immunoprecipitated as described in Methods and studied by SDS-PAGE and autoradiography. M, medium; L, lysate. Antibody +, anti-AOAH; antibody −, preimmune IgG. Only pro-AOAH is seen in the media, whereas the lysate contains both pro-AOAH and mature AOAH.
    Figure Legend Snippet: Proximal tubule cells secrete pro-AOAH. (A) Diagram of AOAH structure, showing the conversion of the precursor (pro-AOAH) into mature AOAH. (B) Production of 35 S-AOAH by porcine proximal tubule cells in vitro. Labeled AOAH was immunoprecipitated as described in Methods and studied by SDS-PAGE and autoradiography. M, medium; L, lysate. Antibody +, anti-AOAH; antibody −, preimmune IgG. Only pro-AOAH is seen in the media, whereas the lysate contains both pro-AOAH and mature AOAH.

    Techniques Used: In Vitro, Labeling, Immunoprecipitation, SDS Page, Autoradiography

    Related Articles

    Blocking Assay:

    Article Title: Hydrogen sulfide mediates the protection of dietary restriction against renal senescence in aged F344 rats
    Article Snippet: .. After dewaxing, antigen retrieval and blocking of endogenous peroxidase, the sections were fixed with goat serum and incubated with a 1:1000 dilution of rabbit monoclonal anti-CGL antibody (Abcam, Cambridge MA, USA), a 1:2000 dilution of rabbit monoclonal anti-CBS antibody (Abcam, Cambridge MA, USA), and a 1:20 dilution of mouse monoclonal anti-8-OHdG antibody (Santa cruz biotechnology, CA, USA) overnight at 4 °C, followed by incubation with biotin-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) and streptavidin- conjugated peroxidase (Invitrogen, Carlsbad, CA, USA). .. Finally, sections were detected under a microscope after reaction using 3,3′-diaminobenzidine (DAB) (Invitrogen, Carlsbad, CA, USA).

    Purification:

    Article Title: Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract
    Article Snippet: .. Wells were blocked for 1 h at 37°C, samples and recombinant human AOAH standard were incubated at 4°C overnight or at 37°C for 2 h, secondary antibody (E553, purified polyclonal rabbit anti-human AOAH, final concentration 10 μg/ml) was added for 1 h at 37°C, biotin-conjugated goat anti-rabbit IgG (0.075 μg/ml) (Zymed, San Francisco, Calif.) was added for 1 h at 37°C, 2 μg of alkaline phosphatase-conjugated streptavidin (Jackson Laboratories)/ml was incubated for 30 min at 37°C, and the plate was developed with alkaline phosphatase substrate (5 mM Sigma 104 and 0.1 M Sigma alkaline buffer 221 in water). .. The optical densities were read at 405 nm (MRX Revelation; Dynex Technologies, Chantilly, Va.).

    Concentration Assay:

    Article Title: Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract
    Article Snippet: .. Wells were blocked for 1 h at 37°C, samples and recombinant human AOAH standard were incubated at 4°C overnight or at 37°C for 2 h, secondary antibody (E553, purified polyclonal rabbit anti-human AOAH, final concentration 10 μg/ml) was added for 1 h at 37°C, biotin-conjugated goat anti-rabbit IgG (0.075 μg/ml) (Zymed, San Francisco, Calif.) was added for 1 h at 37°C, 2 μg of alkaline phosphatase-conjugated streptavidin (Jackson Laboratories)/ml was incubated for 30 min at 37°C, and the plate was developed with alkaline phosphatase substrate (5 mM Sigma 104 and 0.1 M Sigma alkaline buffer 221 in water). .. The optical densities were read at 405 nm (MRX Revelation; Dynex Technologies, Chantilly, Va.).

    Incubation:

    Article Title: Two novel STK11 missense mutations induce phosphorylation of S6K and promote cell proliferation in Peutz-Jeghers syndrome
    Article Snippet: .. After three washes with PBST (2,000 ml PBS+4 ml Tween 20), the sections were incubated with biotin-conjugated goat anti-rabbit immunoglobulin G (1:300; catalog no. SPN-9001; Invitrogen; Thermo Fisher Scientific, Inc.) for 10 min at room temperature, and then incubated with streptavidin-peroxidase for 10 min at room temperature. .. A known p-S6 positive colorectal cancer tissue, from one patient who had undergone endoscopic at Nanfang Hospital.

    Article Title: Hydrogen sulfide mediates the protection of dietary restriction against renal senescence in aged F344 rats
    Article Snippet: .. After dewaxing, antigen retrieval and blocking of endogenous peroxidase, the sections were fixed with goat serum and incubated with a 1:1000 dilution of rabbit monoclonal anti-CGL antibody (Abcam, Cambridge MA, USA), a 1:2000 dilution of rabbit monoclonal anti-CBS antibody (Abcam, Cambridge MA, USA), and a 1:20 dilution of mouse monoclonal anti-8-OHdG antibody (Santa cruz biotechnology, CA, USA) overnight at 4 °C, followed by incubation with biotin-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) and streptavidin- conjugated peroxidase (Invitrogen, Carlsbad, CA, USA). .. Finally, sections were detected under a microscope after reaction using 3,3′-diaminobenzidine (DAB) (Invitrogen, Carlsbad, CA, USA).

    Article Title: Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract
    Article Snippet: .. Wells were blocked for 1 h at 37°C, samples and recombinant human AOAH standard were incubated at 4°C overnight or at 37°C for 2 h, secondary antibody (E553, purified polyclonal rabbit anti-human AOAH, final concentration 10 μg/ml) was added for 1 h at 37°C, biotin-conjugated goat anti-rabbit IgG (0.075 μg/ml) (Zymed, San Francisco, Calif.) was added for 1 h at 37°C, 2 μg of alkaline phosphatase-conjugated streptavidin (Jackson Laboratories)/ml was incubated for 30 min at 37°C, and the plate was developed with alkaline phosphatase substrate (5 mM Sigma 104 and 0.1 M Sigma alkaline buffer 221 in water). .. The optical densities were read at 405 nm (MRX Revelation; Dynex Technologies, Chantilly, Va.).

    Article Title: Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila
    Article Snippet: .. The secondary antibody solution with the fly brain was incubated at room temperature overnight, and the dilution ratio was 1:200 for biotin-conjugated goat anti-mouse IgG (Invitrogen) and biotin-conjugated goat anti-rabbit IgG (Invitrogen), and then the fly brain was labeled by 1:200 dilution with Alexa Fluor 635 streptavidin (Invitrogen) at room temperature overnight. .. Statistics All of the behavioral raw data were analyzed parametrically with JMP® 9.0.0 software.

    Article Title: Dual Roles for c-Jun N-Terminal Kinase in Developmental and Stress Responses in Cerebellar Granule Neurons
    Article Snippet: .. Incubation overnight with primary antibody 1:2000 rabbit anti-β-galactosidase (5′→3′ Inc., Boulder, CO) was followed by 1 hr incubation with biotin-conjugated goat anti-rabbit IgG and then 1:500 streptavidin Alexa-488 (Molecular Probes, Leiden, The Netherlands). .. Cerebellar granule neurons transfected with pCMV-β-galactosidase for the morphological studies were fixed 48 hr after transfection and stained as outlined above.

    Labeling:

    Article Title: Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila
    Article Snippet: .. The secondary antibody solution with the fly brain was incubated at room temperature overnight, and the dilution ratio was 1:200 for biotin-conjugated goat anti-mouse IgG (Invitrogen) and biotin-conjugated goat anti-rabbit IgG (Invitrogen), and then the fly brain was labeled by 1:200 dilution with Alexa Fluor 635 streptavidin (Invitrogen) at room temperature overnight. .. Statistics All of the behavioral raw data were analyzed parametrically with JMP® 9.0.0 software.

    Recombinant:

    Article Title: Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract
    Article Snippet: .. Wells were blocked for 1 h at 37°C, samples and recombinant human AOAH standard were incubated at 4°C overnight or at 37°C for 2 h, secondary antibody (E553, purified polyclonal rabbit anti-human AOAH, final concentration 10 μg/ml) was added for 1 h at 37°C, biotin-conjugated goat anti-rabbit IgG (0.075 μg/ml) (Zymed, San Francisco, Calif.) was added for 1 h at 37°C, 2 μg of alkaline phosphatase-conjugated streptavidin (Jackson Laboratories)/ml was incubated for 30 min at 37°C, and the plate was developed with alkaline phosphatase substrate (5 mM Sigma 104 and 0.1 M Sigma alkaline buffer 221 in water). .. The optical densities were read at 405 nm (MRX Revelation; Dynex Technologies, Chantilly, Va.).

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  • 99
    Thermo Fisher biotin conjugated goat pab against rabbit igg h l
    Biotin Conjugated Goat Pab Against Rabbit Igg H L, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat pab against rabbit igg h l/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat pab against rabbit igg h l - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher biotin conjugated goat anti rabbit igg
    Biotin Conjugated Goat Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat anti rabbit igg/product/Thermo Fisher
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit igg - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    Thermo Fisher pierce goat anti rabbit igg h l biotin conjugated secondary antibody
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    https://www.bioz.com/result/pierce goat anti rabbit igg h l biotin conjugated secondary antibody/product/Thermo Fisher
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