biotin conjugated f  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure F ab 2 Fragment Donkey Anti Chicken IgY IgG
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule chicken IgY It also reacts with the light chains of other chicken immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine goat guinea pig syrian hamster horse human mouse rabbit rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    703-066-155
    Price:
    137
    Purity:
    The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Donkey
    Quantity:
    0 3
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin conjugated f
    Biotin SP AffiniPure F ab 2 Fragment Donkey Anti Chicken IgY IgG
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule chicken IgY It also reacts with the light chains of other chicken immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine goat guinea pig syrian hamster horse human mouse rabbit rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin conjugated f/product/Jackson Immuno
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated f - by Bioz Stars, 2020-07
    90/100 stars

    Images

    Related Articles

    Transduction:

    Article Title: Fluorine-19 nuclear magnetic resonance of chimeric antigen receptor T cell biodistribution in murine cancer model
    Article Snippet: .. The T cell transduction efficacy was determined by flow cytometry at 5 and 14 days after virus addition using a primary biotin-SP-AffiniPure F(ab’)2 fragment-specific goat anti-mouse antibody (Jackson Immuno Research Laboratories, West Grove, PA) and streptavidin-PE served as the secondary antibody (BD Pharmingen, San Diego, CA). .. To label T cells for NMR cytometry, CAR T cells and untransduced T cells (control) were plated at a density of 10 million cells in 5 ml of RPMI in 6-well plates and incubated for 12 h with 10 mg/ml of PFC nanoemulsion (CS-1000 or CS-ATM DM Red, Celsense, Inc., Pittsburgh, PA).

    Article Title: Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity
    Article Snippet: .. LV-CAR transduction efficiency was assessed by flow cytometry staining cells with biotin AffiniPure F(ab’) fragment goat anti-mouse immunoglobulin IgG (Jackson Immunoresearch, West Grove, PA, USA) followed by streptavidin-APC (Biolegend, San Diego, CA). .. CXCL10 ELISA Cell culture supernatants from HEK293T cells grown in 6-well plates were assayed at 48 h post transfection for CXCL10 protein using Duoset enzyme-linked immunosorbent assay (ELISA) reagents (R & D Systems) according to the manufacturer’s instructions.

    Staining:

    Article Title: The Rate of CD4 T Cell Entry into the Lungs during Mycobacterium tuberculosis Infection Is Determined by Partial and Opposing Effects of Multiple Chemokine Receptors
    Article Snippet: .. The cells treated with human anti-KLRG1 IgG1 (kindly provided by Abcuro, Newton MA) were stained with a biotin-SP AffiniPure F(ab′)2 fragment donkey anti-human IgG, Fcγ fragment-specific antibody purchased from Jackson ImmunoResearch Laboratory (West Grove, PA) and secondarily stained with fluorochrome-labeled streptavidin. .. Samples were acquired on an LSR II Fortessa flow cytometer and analyzed using FlowJo software (BD Biosciences, San Jose, CA).

    Article Title: Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity
    Article Snippet: .. LV-CAR transduction efficiency was assessed by flow cytometry staining cells with biotin AffiniPure F(ab’) fragment goat anti-mouse immunoglobulin IgG (Jackson Immunoresearch, West Grove, PA, USA) followed by streptavidin-APC (Biolegend, San Diego, CA). .. CXCL10 ELISA Cell culture supernatants from HEK293T cells grown in 6-well plates were assayed at 48 h post transfection for CXCL10 protein using Duoset enzyme-linked immunosorbent assay (ELISA) reagents (R & D Systems) according to the manufacturer’s instructions.

    Flow Cytometry:

    Article Title: Fluorine-19 nuclear magnetic resonance of chimeric antigen receptor T cell biodistribution in murine cancer model
    Article Snippet: .. The T cell transduction efficacy was determined by flow cytometry at 5 and 14 days after virus addition using a primary biotin-SP-AffiniPure F(ab’)2 fragment-specific goat anti-mouse antibody (Jackson Immuno Research Laboratories, West Grove, PA) and streptavidin-PE served as the secondary antibody (BD Pharmingen, San Diego, CA). .. To label T cells for NMR cytometry, CAR T cells and untransduced T cells (control) were plated at a density of 10 million cells in 5 ml of RPMI in 6-well plates and incubated for 12 h with 10 mg/ml of PFC nanoemulsion (CS-1000 or CS-ATM DM Red, Celsense, Inc., Pittsburgh, PA).

    Article Title: Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity
    Article Snippet: .. LV-CAR transduction efficiency was assessed by flow cytometry staining cells with biotin AffiniPure F(ab’) fragment goat anti-mouse immunoglobulin IgG (Jackson Immunoresearch, West Grove, PA, USA) followed by streptavidin-APC (Biolegend, San Diego, CA). .. CXCL10 ELISA Cell culture supernatants from HEK293T cells grown in 6-well plates were assayed at 48 h post transfection for CXCL10 protein using Duoset enzyme-linked immunosorbent assay (ELISA) reagents (R & D Systems) according to the manufacturer’s instructions.

    Cytometry:

    Article Title: Fluorine-19 nuclear magnetic resonance of chimeric antigen receptor T cell biodistribution in murine cancer model
    Article Snippet: .. The T cell transduction efficacy was determined by flow cytometry at 5 and 14 days after virus addition using a primary biotin-SP-AffiniPure F(ab’)2 fragment-specific goat anti-mouse antibody (Jackson Immuno Research Laboratories, West Grove, PA) and streptavidin-PE served as the secondary antibody (BD Pharmingen, San Diego, CA). .. To label T cells for NMR cytometry, CAR T cells and untransduced T cells (control) were plated at a density of 10 million cells in 5 ml of RPMI in 6-well plates and incubated for 12 h with 10 mg/ml of PFC nanoemulsion (CS-1000 or CS-ATM DM Red, Celsense, Inc., Pittsburgh, PA).

    Article Title: Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity
    Article Snippet: .. LV-CAR transduction efficiency was assessed by flow cytometry staining cells with biotin AffiniPure F(ab’) fragment goat anti-mouse immunoglobulin IgG (Jackson Immunoresearch, West Grove, PA, USA) followed by streptavidin-APC (Biolegend, San Diego, CA). .. CXCL10 ELISA Cell culture supernatants from HEK293T cells grown in 6-well plates were assayed at 48 h post transfection for CXCL10 protein using Duoset enzyme-linked immunosorbent assay (ELISA) reagents (R & D Systems) according to the manufacturer’s instructions.

    Incubation:

    Article Title: Metabolic Phenotypes Of Response to Vaccination in Humans
    Article Snippet: .. The next day, the plates were washed four times with PBS, four times with PBS-T and incubated for 1.5 hr at room temperature with Biotin-SP-Affinipure F(ab′)2 donkey anti-human IgG (Jackson Immunoresearch) at 100 ng/well in PBS + 10% FCS + 0.05% Tween 20 (PBS-T-FBS). .. Plates were washed four times with PBS-T and incubated for 1.5 hr with HRP-avidin D (Vector laboratories) at 1:1000 in PBS-T-FBS.

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