biotin avidin peroxidase complex solution  (Vector Laboratories)


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    Name:
    VECTASTAIN Elite ABC HRP Reagent R T U Peroxidase Ready to Use
    Description:
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Component The R T U VECTASTAIN Elite ABC reagent consists of 50 ml stabilized prediluted and preformed Elite ABC reagent The R T U kits are ideal for manual or automated tissue staining systems The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    pk-7100
    Price:
    None
    Size:
    50 ml
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Buy from Supplier


    Structured Review

    Vector Laboratories biotin avidin peroxidase complex solution
    VECTASTAIN Elite ABC HRP Reagent R T U Peroxidase Ready to Use
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Component The R T U VECTASTAIN Elite ABC reagent consists of 50 ml stabilized prediluted and preformed Elite ABC reagent The R T U kits are ideal for manual or automated tissue staining systems The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/biotin avidin peroxidase complex solution/product/Vector Laboratories
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin avidin peroxidase complex solution - by Bioz Stars, 2020-09
    99/100 stars

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    Incubation:

    Article Title: MUC16/CA125 in the Context of Modular Proteins with an Annotated Role in Adhesion-Related Processes: In Silico Analysis
    Article Snippet: .. Subsequent to incubation for 1 h, the wells were rinsed and Vectastain Elite ABC reagent (Vector Laboratories, Burlinghame, CA, USA) was added followed by incubation for 30 min. After another washing step, addition of TMB substrate solution and incubation for 10 min, the reaction was stopped with 0.16 M H2 SO4 . ..

    Article Title: Fibrin deposited in the Alzheimer’s disease brain promotes neuronal degeneration
    Article Snippet: .. The following morning, sections were incubated with a biotinylated horse anti-mouse antibody, amplified by the VECTASTAIN Elite ABC Ready-to-Use Reagent, and developed using ImmPACT DAB Peroxidase Substrate (all from Vector Labs). .. Sections were then dehydrated, mounted, and imaged using a Zeiss Axiovert 200 microscope.

    Article Title: Tumor-Associated Macrophages Are a Useful Biomarker to Predict Recurrence After Surgical Resection of Non-Functional Pancreatic Neuroendocrine Tumors
    Article Snippet: .. For detection, slides were incubated with biotinylated goat anti-mouse immunoglobulin G (1:300 dilution, Vector Labs; Burlingame, CA), detected with VECTASTAIN Elite ABC Reagent (Vector Labs; Burlingame, CA) and Vector DAB substrate (Vector Labs; Burlingame, CA), and counterstained with hematoxylin (Sigma-Aldrich; St. Louis, MO). .. CD68 staining was scored blindly from 1-3 for degree of tumor-associated macrophage infiltration as previously described.

    Article Title: The forkhead transcription factor Foxo1 links insulin signaling to Pdx1 regulation of pancreatic ? cell growth
    Article Snippet: .. We incubated the sections sequentially with biotinylated anti-mouse IgG reagent (Vector Laboratories Inc., Burlingame, California, USA), VECTASTAIN Elite ABC reagent and DAB (Vector Laboratories Inc.) for visualization. .. For double staining with anti-BrdU and anti-insulin antibodies, we used a guinea pig anti-insulin antibody.

    Amplification:

    Article Title: Fibrin deposited in the Alzheimer’s disease brain promotes neuronal degeneration
    Article Snippet: .. The following morning, sections were incubated with a biotinylated horse anti-mouse antibody, amplified by the VECTASTAIN Elite ABC Ready-to-Use Reagent, and developed using ImmPACT DAB Peroxidase Substrate (all from Vector Labs). .. Sections were then dehydrated, mounted, and imaged using a Zeiss Axiovert 200 microscope.

    Binding Assay:

    Article Title: Transgenic expression of survivin in keratinocytes counteracts UVB-induced apoptosis and cooperates with loss of p53
    Article Snippet: .. Binding of the primary Ab was visualized using a goat anti-rabbit polyclonal Ab (1:100) and Vectastain Elite ABC and 3-amino-9-ethyl carbazole (AEC) peroxidase substrate kits (Vector Laboratories) according to the manufacturer’s instructions. ..

    Plasmid Preparation:

    Article Title: von Willebrand factor deficiency does not influence angiotensin II-induced abdominal aortic aneurysm formation in mice
    Article Snippet: .. After washing, both the VECTASTAIN Elite ABC-HRP Kit (Vector Laboratories) and the VECTOR VIP Peroxidase Substrate Kit (Vector Laboratories) were used for detection according to the manufacturer’s instructions. .. Counterstaining was performed using VECTOR Methyl Green (Vector Laboratories) according to the manufacturer’s instructions.

    Article Title: Tumor-Associated Macrophages Are a Useful Biomarker to Predict Recurrence After Surgical Resection of Non-Functional Pancreatic Neuroendocrine Tumors
    Article Snippet: .. For detection, slides were incubated with biotinylated goat anti-mouse immunoglobulin G (1:300 dilution, Vector Labs; Burlingame, CA), detected with VECTASTAIN Elite ABC Reagent (Vector Labs; Burlingame, CA) and Vector DAB substrate (Vector Labs; Burlingame, CA), and counterstained with hematoxylin (Sigma-Aldrich; St. Louis, MO). .. CD68 staining was scored blindly from 1-3 for degree of tumor-associated macrophage infiltration as previously described.

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  • 91
    Vector Laboratories avidin biotin peroxidase complex solution
    Avidin Biotin Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex solution/product/Vector Laboratories
    Average 91 stars, based on 118 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex solution - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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