biosprint96  (Qiagen)


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    Name:
    BioSprint 96 DNA Blood Kit
    Description:
    For rapid and economical automated purification of DNA from a wide range of samples. Kit contents: For 48 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents. Benefits: Complete removal of contamination and inhibitors. Purification of high-quality DNA that is ready for use. Automated procedure saves time and effort. No organic extraction or alcohol precipita
    Catalog Number:
    940054
    Price:
    None
    Category:
    BioSprint 96 DNA Blood Kit
    Score:
    85
    Buy from Supplier


    Structured Review

    Qiagen biosprint96
    BioSprint 96 DNA Blood Kit
    For rapid and economical automated purification of DNA from a wide range of samples. Kit contents: For 48 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents. Benefits: Complete removal of contamination and inhibitors. Purification of high-quality DNA that is ready for use. Automated procedure saves time and effort. No organic extraction or alcohol precipita
    https://www.bioz.com/result/biosprint96/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biosprint96 - by Bioz Stars, 2019-12
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Genetic attributes of midwife toad (Alytes obstetricans) populations do not correlate with degree of species decline
    Article Snippet: Paragraph title: Microsatellite amplification ... We extracted DNA from tail clips using the BioSprint 96 DNA Blood Kit (Qiagen, Hombrechtikon, Switzerland), following the protocol for tissue extraction.

    Article Title: Identifying avian malaria vectors: sampling methods influence outcomes
    Article Snippet: DNA was then extracted from thoraxes following the Qiagen BioSprint 96 DNA Tissue Extraction Kit (Qiagen, Valencia, CA) protocol and using the BioSprint 96 instrument (Qiagen, Valencia, CA). .. Extracted DNA from mosquito thoraxes was screened for both Plasmodium and Haemoproteus parasite DNA using polymerase chain reactions (PCR).

    Article Title: Out of the Reservoir: Phenotypic and Genotypic Characterization of a Novel Cowpox Virus Isolated from a Common Vole
    Article Snippet: DNA was extracted using a BioSprint 96 instrument and a MagAttract Virus Mini M48 kit (both, Qiagen, Hilden, Germany) from liver samples of individual rodents, and an orthopoxvirus (OPV)-specific quantitative PCR (qPCR) assay was applied ( ). .. DNA was extracted using a BioSprint 96 instrument and a MagAttract Virus Mini M48 kit (both, Qiagen, Hilden, Germany) from liver samples of individual rodents, and an orthopoxvirus (OPV)-specific quantitative PCR (qPCR) assay was applied ( ).

    Article Title: Landscape limits gene flow and drives population structure in Agassiz’s desert tortoise (Gopherus agassizii)
    Article Snippet: Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA). .. Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA).

    Picogreen Assay:

    Article Title: Genomics of extreme ecological specialists: multiple convergent evolution but no genetic divergence between ecotypes of Maculinea alcon butterflies
    Article Snippet: For most of the samples, whole genomic DNA was extracted from the thorax or abdomen, using BioSprint 96 robot with BioSprint 96 DNA Blood Kit (Qiagen, Hilden, Germany). .. For most of the samples, whole genomic DNA was extracted from the thorax or abdomen, using BioSprint 96 robot with BioSprint 96 DNA Blood Kit (Qiagen, Hilden, Germany).

    Electrophoresis:

    Article Title: Diagnostic PCR assays to unravel food web interactions in cereal crops with focus on biological control of aphids
    Article Snippet: DNA was subsequently extracted using the BioSprint 96 DNA blood Kit (Qiagen, Hilden, Germany) running on the BioSprint 96 instrument (Qiagen) in accordance to the manufacturer’s instructions, yielding 200 µl of DNA extract per sample. .. Amplifications were performed with standard singleplex PCR chemistry (OneTaq ® DNA polymerase, 50 °C annealing temperature, for details see ESM 2) following the thermocycling recommendations of the manufacturer and carried out in a Mastercycler Gradient (Eppendorf, Hamburg, Germany).

    Article Title: Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection
    Article Snippet: For PCR, genomic DNA was isolated from A. gambiae abdominal tissue using the Qiagen BioSprint 96 (Valencia, CA) following the manufacturer's protocol. .. Cycling conditions were as follows: 94°C for 2 min; 2 cycles of 94°C for 30 sec, 62°C for 30 sec, 72°C for 30 sec; 6 cycles of 94°C for 30 sec, 55-61°C (the range of target-specific annealing temperatures) for 30 sec, 72°C for 30 sec; 27 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec, followed by a 7 minute extension at 72°C.

    Incubation:

    Article Title: Individual Differences in Scanpaths Correspond with Serotonin Transporter Genotype and Behavioral Phenotype in Rhesus Monkeys (Macaca mulatta)
    Article Snippet: Immediately prior to extraction the lysis buffer volumes (with the swab head) were normalized to 1 ml, 25 μl of proteinase K (20 mg/ml) was added to all samples, and they were incubated with agitation at 55°C overnight. .. DNA was isolated from the samples using a protocol modified from the Qiagen BioSprint 96 DNA Blood Kit as follows: The lysate was loaded into two identical 2.2 ml deep well plates.

    Article Title: Diagnostic PCR assays to unravel food web interactions in cereal crops with focus on biological control of aphids
    Article Snippet: Tissue samples were lysed in 430 µl TES buffer (0.1 M TRIS, 10 mM EDTA, 2 % SDS; pH 8) and 10 µl Proteinase K (20 mg ml−1 ), homogenised with 3 mm glass beads for 1 min at 5000 rpm using a Precellys® 24 Tissue Homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France), and incubated overnight at 58 °C. .. DNA was subsequently extracted using the BioSprint 96 DNA blood Kit (Qiagen, Hilden, Germany) running on the BioSprint 96 instrument (Qiagen) in accordance to the manufacturer’s instructions, yielding 200 µl of DNA extract per sample.

    Article Title: Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States
    Article Snippet: The kidney samples were homogenized as described above and 500 µL of the supernatant from the kidney homogenate was added to 9.5 mL P80-BA liquid oleic media. (National Veterinary Services Laboratory, Ames, IA).. Three serial 10-fold dilutions (10−2 , 10−3 , and 10−4 ) were prepared, and 100 µL from the 10−2 dilution was inoculated into two semisolid P-80-BA liquid oleic media tubes with 5-fluorouracil and two without 5-fluorouracil.The tubes were incubated at 29 °C up to 6 months. .. DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations.

    Article Title: Identifying avian malaria vectors: sampling methods influence outcomes
    Article Snippet: Thoraxes of each individual mosquito were homogenized using the Qiagen stainless steel 5 mm beads and the Qiagen TissueLyser (Qiagen, Valencia, CA) for 3 min at the maximum speed before the overnight incubation in the lysis buffer provided in the kit. .. DNA was then extracted from thoraxes following the Qiagen BioSprint 96 DNA Tissue Extraction Kit (Qiagen, Valencia, CA) protocol and using the BioSprint 96 instrument (Qiagen, Valencia, CA).

    Article Title: Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples, et al. Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples
    Article Snippet: The CTAB extraction was performed as follows: 60 μl of 5 mol/L NaCl and 25 μl of 10% CTAB (cetyltrimethylammonium bromide) solution were added to the 200 μl lysates and incubated for 10 min at 65°C. .. The automated DNA extraction was performed using the BioSprint® 96 DNA Blood Kit on a BioSprint® 96 extraction robotic platform (Qiagen).

    Cell Culture:

    Article Title: Co-expression Analysis of Sirtuins and Related Metabolic Biomarkers in Juveniles of Gilthead Sea Bream (Sparus aurata) With Differences in Growth Performance
    Article Snippet: Briefly, DNA was extracted from the fin by using the BioSprint 96 DNA Blood Kit (QIAGEN®) operated by a Biosprint 96 robot. .. Briefly, DNA was extracted from the fin by using the BioSprint 96 DNA Blood Kit (QIAGEN®) operated by a Biosprint 96 robot.

    Expressing:

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Expression products were affinity purified via nickel-agarose (Qiagen) under denaturing conditions using 8 M urea. .. Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen).

    Modification:

    Article Title: Individual Differences in Scanpaths Correspond with Serotonin Transporter Genotype and Behavioral Phenotype in Rhesus Monkeys (Macaca mulatta)
    Article Snippet: Immediately prior to extraction the lysis buffer volumes (with the swab head) were normalized to 1 ml, 25 μl of proteinase K (20 mg/ml) was added to all samples, and they were incubated with agitation at 55°C overnight. .. DNA was isolated from the samples using a protocol modified from the Qiagen BioSprint 96 DNA Blood Kit as follows: The lysate was loaded into two identical 2.2 ml deep well plates. .. Each plate contained 300 μl lysate, 300 μl Isopropanol, and 300 μl Qiagen buffer AL.

    Article Title: Elevation in tropical sky islands as the common driver in structuring genes and communities of freshwater organisms
    Article Snippet: DNA extractions were performed using a Biosprint 96 extraction robot (Qiagen, Hombrechtikon, Switzerland), following the manufacturer’s protocol. .. DNA extractions were performed using a Biosprint 96 extraction robot (Qiagen, Hombrechtikon, Switzerland), following the manufacturer’s protocol.

    Flow Cytometry:

    Article Title: Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples, et al. Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples
    Article Snippet: The automated DNA extraction was performed using the BioSprint® 96 DNA Blood Kit on a BioSprint® 96 extraction robotic platform (Qiagen). .. DNA extracts were stored at −28°C until PCR.

    Ligation:

    Article Title: Landscape limits gene flow and drives population structure in Agassiz’s desert tortoise (Gopherus agassizii)
    Article Snippet: Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA). .. Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA).

    Infection:

    Article Title: Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection
    Article Snippet: For PCR, genomic DNA was isolated from A. gambiae abdominal tissue using the Qiagen BioSprint 96 (Valencia, CA) following the manufacturer's protocol. .. Amplimer sizes were confirmed by electrophoresis prior to purification with Exo SapIT ([ ]; USB, Cleveland, OH) for DNA sequencing.

    DNA Sequencing:

    Article Title: Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection
    Article Snippet: For PCR, genomic DNA was isolated from A. gambiae abdominal tissue using the Qiagen BioSprint 96 (Valencia, CA) following the manufacturer's protocol. .. Cycling conditions were as follows: 94°C for 2 min; 2 cycles of 94°C for 30 sec, 62°C for 30 sec, 72°C for 30 sec; 6 cycles of 94°C for 30 sec, 55-61°C (the range of target-specific annealing temperatures) for 30 sec, 72°C for 30 sec; 27 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec, followed by a 7 minute extension at 72°C.

    Sequencing:

    Article Title: Diagnostic PCR assays to unravel food web interactions in cereal crops with focus on biological control of aphids
    Article Snippet: Paragraph title: Compilation of sequence databases for primer design ... DNA was subsequently extracted using the BioSprint 96 DNA blood Kit (Qiagen, Hilden, Germany) running on the BioSprint 96 instrument (Qiagen) in accordance to the manufacturer’s instructions, yielding 200 µl of DNA extract per sample.

    Article Title: Hidden in plain sight: Cryptic and endemic malaria parasites in North American white-tailed deer (Odocoileus virginianus)
    Article Snippet: DNA was extracted from blood or tissues using a Qiagen DNeasy Blood and Tissue Kit or the Qiagen BioSprint 96 System following the manufacturer’s guidelines. .. DNA was extracted from blood or tissues using a Qiagen DNeasy Blood and Tissue Kit or the Qiagen BioSprint 96 System following the manufacturer’s guidelines.

    Article Title: Elevation in tropical sky islands as the common driver in structuring genes and communities of freshwater organisms
    Article Snippet: Paragraph title: RAD sequencing ... DNA extractions were performed using a Biosprint 96 extraction robot (Qiagen, Hombrechtikon, Switzerland), following the manufacturer’s protocol.

    Affinity Purification:

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Expression products were affinity purified via nickel-agarose (Qiagen) under denaturing conditions using 8 M urea. .. Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen).

    Binding Assay:

    Article Title: Individual Differences in Scanpaths Correspond with Serotonin Transporter Genotype and Behavioral Phenotype in Rhesus Monkeys (Macaca mulatta)
    Article Snippet: DNA was isolated from the samples using a protocol modified from the Qiagen BioSprint 96 DNA Blood Kit as follows: The lysate was loaded into two identical 2.2 ml deep well plates. .. DNA was isolated from the samples using a protocol modified from the Qiagen BioSprint 96 DNA Blood Kit as follows: The lysate was loaded into two identical 2.2 ml deep well plates.

    DNA Extraction:

    Article Title: Individual Differences in Scanpaths Correspond with Serotonin Transporter Genotype and Behavioral Phenotype in Rhesus Monkeys (Macaca mulatta)
    Article Snippet: Paragraph title: DNA extraction and genotyping ... DNA was isolated from the samples using a protocol modified from the Qiagen BioSprint 96 DNA Blood Kit as follows: The lysate was loaded into two identical 2.2 ml deep well plates.

    Article Title: Asymmetric introgression between the M and S forms of the malaria vector, Anopheles gambiae, maintains divergence despite extensive hybridisation
    Article Snippet: All samples were stored in individual tubes containing 70% ethanol. .. DNA was extracted from specimens using DNeasy blood and tissue kit (Qiagen, Valencia, CA) using the Qiagen Biosprint 96 DNA extraction system. .. The DNA samples were subsequently amplified with whole genome amplification Repli-G kits (Qiagen, Valencia, CA) to raise DNA concentrations to 50µg/µl for downstream analyses.

    Article Title: Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States
    Article Snippet: An aliquot of the homogenate was stored in a −70 °C freezer for PCR. .. DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations. .. Real-time PCR was conducted using a previously described protocol.

    Article Title: The Genetic Basis of Host Preference and Resting Behavior in the Major African Malaria Vector, Anopheles arabiensis
    Article Snippet: Paragraph title: DNA extraction ... For each specimen, the abdomen was separated from the head and thorax and DNA was extracted separately from each using the QIAGEN Biosprint 96 system and QIAGEN blood and tissue kits (QIAGEN, Valencia, CA).

    Article Title: Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples, et al. Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples
    Article Snippet: 350 μl) at −28°C overnight, and samples were centrifuged for 20 min at 4°C 21,000 g , washed with 300 μl 70% chilled ethanol, and again centrifuged at 4°C 21,000 g for 15 min. After removing the ethanol and air‐drying the pellet, DNA was finally resuspended in 200 μl 1 × TE (10 mmol/L TRIS, 1 mmol/L EDTA, pH 8.0) and stored at −28°C. .. The automated DNA extraction was performed using the BioSprint® 96 DNA Blood Kit on a BioSprint® 96 extraction robotic platform (Qiagen). .. The kit combines silica‐based DNA purification with paramagnetic beads, where DNA binds to the silica surface of the paramagnetic particles in the presence of a chaotropic salt and is then washed repeatedly, making use of the same chemistry as the silica‐column‐based DNeasy Blood & Tissue Kit (Qiagen).

    Nucleic Acid Electrophoresis:

    Article Title: Hidden in plain sight: Cryptic and endemic malaria parasites in North American white-tailed deer (Odocoileus virginianus)
    Article Snippet: DNA was extracted from blood or tissues using a Qiagen DNeasy Blood and Tissue Kit or the Qiagen BioSprint 96 System following the manufacturer’s guidelines. .. DNA was extracted from blood or tissues using a Qiagen DNeasy Blood and Tissue Kit or the Qiagen BioSprint 96 System following the manufacturer’s guidelines.

    Fluorescence:

    Article Title: Genomics of extreme ecological specialists: multiple convergent evolution but no genetic divergence between ecotypes of Maculinea alcon butterflies
    Article Snippet: For most of the samples, whole genomic DNA was extracted from the thorax or abdomen, using BioSprint 96 robot with BioSprint 96 DNA Blood Kit (Qiagen, Hilden, Germany). .. For most of the samples, whole genomic DNA was extracted from the thorax or abdomen, using BioSprint 96 robot with BioSprint 96 DNA Blood Kit (Qiagen, Hilden, Germany).

    Article Title: Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States
    Article Snippet: DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations. .. DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations.

    Isolation:

    Article Title: Individual Differences in Scanpaths Correspond with Serotonin Transporter Genotype and Behavioral Phenotype in Rhesus Monkeys (Macaca mulatta)
    Article Snippet: Immediately prior to extraction the lysis buffer volumes (with the swab head) were normalized to 1 ml, 25 μl of proteinase K (20 mg/ml) was added to all samples, and they were incubated with agitation at 55°C overnight. .. DNA was isolated from the samples using a protocol modified from the Qiagen BioSprint 96 DNA Blood Kit as follows: The lysate was loaded into two identical 2.2 ml deep well plates. .. Each plate contained 300 μl lysate, 300 μl Isopropanol, and 300 μl Qiagen buffer AL.

    Article Title: Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States
    Article Snippet: An aliquot of the homogenate was stored in a −70 °C freezer for PCR. .. DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations. .. Real-time PCR was conducted using a previously described protocol.

    Article Title: Out of the Reservoir: Phenotypic and Genotypic Characterization of a Novel Cowpox Virus Isolated from a Common Vole
    Article Snippet: CPXV strain FM2292 was isolated from the liver of a female common vole, Microtus arvalis (KS11/2292), collected during a large rodent screening project of the network Rodent-borne Pathogens on a grassland site in the federal state of Baden-Wuerttemberg, Germany, in October 2011 ( ). .. DNA was extracted using a BioSprint 96 instrument and a MagAttract Virus Mini M48 kit (both, Qiagen, Hilden, Germany) from liver samples of individual rodents, and an orthopoxvirus (OPV)-specific quantitative PCR (qPCR) assay was applied ( ).

    Article Title: Avian Influenza H7N9/13 and H7N7/13: a Comparative Virulence Study in Chickens, Pigeons, and Ferrets
    Article Snippet: Paragraph title: RNA isolation. ... Viral RNA was extracted from 200 μl of the supernatant of organ homogenates or swab samples or nasal wash samples with the MagAttract Virus Mini M48 kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions on a Biosprint 96 platform (Qiagen).

    Article Title: Landscape limits gene flow and drives population structure in Agassiz’s desert tortoise (Gopherus agassizii)
    Article Snippet: Fish and Wildlife Service (TE-06556, TE-17730, BO 8-8-11-F-65R), and complied with the Animal Care and Use Committee of the U.S. Geological Survey and the Animal Research Committee at the University of California, Los Angeles. .. Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA). .. The recovered DNA was quantified using a BioTEK Synergy HT (BioTEK, Vermont, USA) and diluted working stocks to 5 ng/μl in Low TE (10 mMTris-pH 8.0, 0.1 mM EDTA).

    Article Title: Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection
    Article Snippet: Primers were designed to amplify fragments of the encoding sequences of the genes of interest in the size range of 120-260 bp, which was optimal for Luminex genotyping (see below). .. For PCR, genomic DNA was isolated from A. gambiae abdominal tissue using the Qiagen BioSprint 96 (Valencia, CA) following the manufacturer's protocol. .. Each amplification reaction contained 1× buffer with MgCl2 (Roche), 67 μM of each dNTP (Applied Biosystems, Foster City, CA), 0.22 μM primers (Sigma-Aldrich, St. Louis, MO), 0.7 U Taq Polymerase (Roche), and 40 ng of genomic DNA.

    Size-exclusion Chromatography:

    Article Title: Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection
    Article Snippet: For PCR, genomic DNA was isolated from A. gambiae abdominal tissue using the Qiagen BioSprint 96 (Valencia, CA) following the manufacturer's protocol. .. Each amplification reaction contained 1× buffer with MgCl2 (Roche), 67 μM of each dNTP (Applied Biosystems, Foster City, CA), 0.22 μM primers (Sigma-Aldrich, St. Louis, MO), 0.7 U Taq Polymerase (Roche), and 40 ng of genomic DNA.

    Microscopy:

    Article Title: Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States
    Article Snippet: The slides were examined under 40 × objective of a fluorescent microscope after applying the mounting fluid and a coverslip. .. DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations.

    Purification:

    Article Title: Diagnostic PCR assays to unravel food web interactions in cereal crops with focus on biological control of aphids
    Article Snippet: DNA was subsequently extracted using the BioSprint 96 DNA blood Kit (Qiagen, Hilden, Germany) running on the BioSprint 96 instrument (Qiagen) in accordance to the manufacturer’s instructions, yielding 200 µl of DNA extract per sample. .. PCR products were separated and visualised using the automated capillary electrophoresis system QIAxcel (Qiagen).

    Article Title: Identifying avian malaria vectors: sampling methods influence outcomes
    Article Snippet: DNA was then extracted from thoraxes following the Qiagen BioSprint 96 DNA Tissue Extraction Kit (Qiagen, Valencia, CA) protocol and using the BioSprint 96 instrument (Qiagen, Valencia, CA). .. We amplified 478 bp (excluding primers) of the parasite mitochondrial cytochrome oxidase subunit-b gene (cyt b ) using nested primers HaemNF, HaemNR2, HaemF and HaemR2 as described in Waldenström et al. [ ].

    Article Title: Hidden in plain sight: Cryptic and endemic malaria parasites in North American white-tailed deer (Odocoileus virginianus)
    Article Snippet: DNA was extracted from blood or tissues using a Qiagen DNeasy Blood and Tissue Kit or the Qiagen BioSprint 96 System following the manufacturer’s guidelines. .. DNA was extracted from blood or tissues using a Qiagen DNeasy Blood and Tissue Kit or the Qiagen BioSprint 96 System following the manufacturer’s guidelines.

    Article Title: Landscape limits gene flow and drives population structure in Agassiz’s desert tortoise (Gopherus agassizii)
    Article Snippet: Fish and Wildlife Service (TE-06556, TE-17730, BO 8-8-11-F-65R), and complied with the Animal Care and Use Committee of the U.S. Geological Survey and the Animal Research Committee at the University of California, Los Angeles. .. Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA). .. The recovered DNA was quantified using a BioTEK Synergy HT (BioTEK, Vermont, USA) and diluted working stocks to 5 ng/μl in Low TE (10 mMTris-pH 8.0, 0.1 mM EDTA).

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Denatured proteins were refolded by stepwise dialysis (Yano et al., ). .. Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen). .. IPD3 was phosphorylated in vitro by DMI3 as described (Jin et al., ).

    Article Title: Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection
    Article Snippet: For PCR, genomic DNA was isolated from A. gambiae abdominal tissue using the Qiagen BioSprint 96 (Valencia, CA) following the manufacturer's protocol. .. Cycling conditions were as follows: 94°C for 2 min; 2 cycles of 94°C for 30 sec, 62°C for 30 sec, 72°C for 30 sec; 6 cycles of 94°C for 30 sec, 55-61°C (the range of target-specific annealing temperatures) for 30 sec, 72°C for 30 sec; 27 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec, followed by a 7 minute extension at 72°C.

    Polymerase Chain Reaction:

    Article Title: Co-expression Analysis of Sirtuins and Related Metabolic Biomarkers in Juveniles of Gilthead Sea Bream (Sparus aurata) With Differences in Growth Performance
    Article Snippet: Both fish populations were recognized as genetically different, with 3.5% of genetic differentiation (Fst = 0.0351), by SMsa1 multiplex PCR of 10 loci (A5, C3, C12, D4, E1, E4, F6, I9, L11, M5) (Lee-Montero et al., ) (Supplementary Table ). .. Briefly, DNA was extracted from the fin by using the BioSprint 96 DNA Blood Kit (QIAGEN®) operated by a Biosprint 96 robot.

    Article Title: Genetic attributes of midwife toad (Alytes obstetricans) populations do not correlate with degree of species decline
    Article Snippet: We extracted DNA from tail clips using the BioSprint 96 DNA Blood Kit (Qiagen, Hombrechtikon, Switzerland), following the protocol for tissue extraction. .. Polymerase chain reactions (PCR) were performed with fluorescent-labeled primers in three separate multiplexes (Table ).

    Article Title: Diagnostic PCR assays to unravel food web interactions in cereal crops with focus on biological control of aphids
    Article Snippet: DNA was subsequently extracted using the BioSprint 96 DNA blood Kit (Qiagen, Hilden, Germany) running on the BioSprint 96 instrument (Qiagen) in accordance to the manufacturer’s instructions, yielding 200 µl of DNA extract per sample. .. DNA was subsequently extracted using the BioSprint 96 DNA blood Kit (Qiagen, Hilden, Germany) running on the BioSprint 96 instrument (Qiagen) in accordance to the manufacturer’s instructions, yielding 200 µl of DNA extract per sample.

    Article Title: Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States
    Article Snippet: DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations. .. DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations.

    Article Title: Identifying avian malaria vectors: sampling methods influence outcomes
    Article Snippet: DNA was then extracted from thoraxes following the Qiagen BioSprint 96 DNA Tissue Extraction Kit (Qiagen, Valencia, CA) protocol and using the BioSprint 96 instrument (Qiagen, Valencia, CA). .. We amplified 478 bp (excluding primers) of the parasite mitochondrial cytochrome oxidase subunit-b gene (cyt b ) using nested primers HaemNF, HaemNR2, HaemF and HaemR2 as described in Waldenström et al. [ ].

    Article Title: Hidden in plain sight: Cryptic and endemic malaria parasites in North American white-tailed deer (Odocoileus virginianus)
    Article Snippet: DNA was extracted from blood or tissues using a Qiagen DNeasy Blood and Tissue Kit or the Qiagen BioSprint 96 System following the manufacturer’s guidelines. .. DNA was extracted from blood or tissues using a Qiagen DNeasy Blood and Tissue Kit or the Qiagen BioSprint 96 System following the manufacturer’s guidelines.

    Article Title: Landscape limits gene flow and drives population structure in Agassiz’s desert tortoise (Gopherus agassizii)
    Article Snippet: Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA). .. Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA).

    Article Title: Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection
    Article Snippet: Primers were designed to amplify fragments of the encoding sequences of the genes of interest in the size range of 120-260 bp, which was optimal for Luminex genotyping (see below). .. For PCR, genomic DNA was isolated from A. gambiae abdominal tissue using the Qiagen BioSprint 96 (Valencia, CA) following the manufacturer's protocol. .. Each amplification reaction contained 1× buffer with MgCl2 (Roche), 67 μM of each dNTP (Applied Biosystems, Foster City, CA), 0.22 μM primers (Sigma-Aldrich, St. Louis, MO), 0.7 U Taq Polymerase (Roche), and 40 ng of genomic DNA.

    Article Title: Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples, et al. Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples
    Article Snippet: The automated DNA extraction was performed using the BioSprint® 96 DNA Blood Kit on a BioSprint® 96 extraction robotic platform (Qiagen). .. The automated DNA extraction was performed using the BioSprint® 96 DNA Blood Kit on a BioSprint® 96 extraction robotic platform (Qiagen).

    Article Title: Elevation in tropical sky islands as the common driver in structuring genes and communities of freshwater organisms
    Article Snippet: DNA extractions were performed using a Biosprint 96 extraction robot (Qiagen, Hombrechtikon, Switzerland), following the manufacturer’s protocol. .. DNA extractions were performed using a Biosprint 96 extraction robot (Qiagen, Hombrechtikon, Switzerland), following the manufacturer’s protocol.

    Staining:

    Article Title: Identifying avian malaria vectors: sampling methods influence outcomes
    Article Snippet: DNA was then extracted from thoraxes following the Qiagen BioSprint 96 DNA Tissue Extraction Kit (Qiagen, Valencia, CA) protocol and using the BioSprint 96 instrument (Qiagen, Valencia, CA). .. PCRs were carried out in a 20 μl reaction mixture using AccuPower Taq PCR PreMix (Bioneer Corporation, Daejeon, Republic of Korea) containing 1 μl of each 10 μM primer, 0.5 μl of Bovine Serum Albumin (BSA), 2 μl of template DNA and 15.5 μl of purified water.

    IA:

    Article Title: Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States
    Article Snippet: The kidney samples were homogenized as described above and 500 µL of the supernatant from the kidney homogenate was added to 9.5 mL P80-BA liquid oleic media. (National Veterinary Services Laboratory, Ames, IA).. Three serial 10-fold dilutions (10−2 , 10−3 , and 10−4 ) were prepared, and 100 µL from the 10−2 dilution was inoculated into two semisolid P-80-BA liquid oleic media tubes with 5-fluorouracil and two without 5-fluorouracil.The tubes were incubated at 29 °C up to 6 months. .. DNA was extracted in an automated DNA extraction unit (BioSprint-96, Qiagen Inc, Valencia, CA) using MagMAXTM total nucleic acid isolation kit (Applied Biosystems, Foster City, CA) using manufacturer’s recommendations.

    Liquid Chromatography:

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen). .. Each reaction was carried out with 1 mg MBP-DMI3 protein, 0.2 mM CaCl2 , 0.5 mM bovine CaM, 2 mg 6XHIS-IPD3 as substrate.

    Software:

    Article Title: Co-expression Analysis of Sirtuins and Related Metabolic Biomarkers in Juveniles of Gilthead Sea Bream (Sparus aurata) With Differences in Growth Performance
    Article Snippet: Briefly, DNA was extracted from the fin by using the BioSprint 96 DNA Blood Kit (QIAGEN®) operated by a Biosprint 96 robot. .. Briefly, DNA was extracted from the fin by using the BioSprint 96 DNA Blood Kit (QIAGEN®) operated by a Biosprint 96 robot.

    Real-time Polymerase Chain Reaction:

    Article Title: Out of the Reservoir: Phenotypic and Genotypic Characterization of a Novel Cowpox Virus Isolated from a Common Vole
    Article Snippet: The trapping of rodents was coordinated by the Julius Kühn-Institut, Münster, Germany, and approved by the responsible authority, the Regierungspräsidium Stuttgart (Landwirtschaft, ländlicher Raum, Veterinär- und Lebensmittelwesen, permit number BW 35-9185.82/0261). .. DNA was extracted using a BioSprint 96 instrument and a MagAttract Virus Mini M48 kit (both, Qiagen, Hilden, Germany) from liver samples of individual rodents, and an orthopoxvirus (OPV)-specific quantitative PCR (qPCR) assay was applied ( ). .. The CPXV rat isolate (RatPox09) was obtained from a diseased pet rat, which had infected two humans in southern Germany in 2009, and had already been characterized by experimental infection of Wistar rats ( ).

    Multiplex Assay:

    Article Title: Co-expression Analysis of Sirtuins and Related Metabolic Biomarkers in Juveniles of Gilthead Sea Bream (Sparus aurata) With Differences in Growth Performance
    Article Snippet: Both fish populations were recognized as genetically different, with 3.5% of genetic differentiation (Fst = 0.0351), by SMsa1 multiplex PCR of 10 loci (A5, C3, C12, D4, E1, E4, F6, I9, L11, M5) (Lee-Montero et al., ) (Supplementary Table ). .. Briefly, DNA was extracted from the fin by using the BioSprint 96 DNA Blood Kit (QIAGEN®) operated by a Biosprint 96 robot.

    Article Title: Genetic attributes of midwife toad (Alytes obstetricans) populations do not correlate with degree of species decline
    Article Snippet: We extracted DNA from tail clips using the BioSprint 96 DNA Blood Kit (Qiagen, Hombrechtikon, Switzerland), following the protocol for tissue extraction. .. Polymerase chain reactions (PCR) were performed with fluorescent-labeled primers in three separate multiplexes (Table ).

    Selection:

    Article Title: Cumulative frequency-dependent selective episodes allow for rapid morph cycles and rock-paper-scissors dynamics in species with overlapping generations
    Article Snippet: Paternity analyses based on four polymorphic microsatellite DNA loci (Lv-3–19, Lv-4–72, Lv-4-alpha, Lv-2–145; [ ]) were used to measure morph siring success (i.e. the number of females with which a male fertilized at least one egg), the principal proxy on which sexual selection acts in Z. vivipara [ ]. .. DNA from tissue samples and eggs was extracted using a BioSprint 96 DNA Blood Kit (Qiagen, Hilden, Germany).

    In Vitro:

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Paragraph title: In vitro phosphorylation and mass spectrometric analysis ... Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen).

    Next-Generation Sequencing:

    Article Title: Landscape limits gene flow and drives population structure in Agassiz’s desert tortoise (Gopherus agassizii)
    Article Snippet: Paragraph title: Laboratory procedures and next-generation sequencing ... Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA).

    Preserving:

    Article Title: Elevation in tropical sky islands as the common driver in structuring genes and communities of freshwater organisms
    Article Snippet: Samples were digested overnight with a proteinase K solution at 56 °C, allowing the preservation of the exoskeleton for microscopic mountings. .. DNA extractions were performed using a Biosprint 96 extraction robot (Qiagen, Hombrechtikon, Switzerland), following the manufacturer’s protocol.

    Activation Assay:

    Article Title: Genetic attributes of midwife toad (Alytes obstetricans) populations do not correlate with degree of species decline
    Article Snippet: We extracted DNA from tail clips using the BioSprint 96 DNA Blood Kit (Qiagen, Hombrechtikon, Switzerland), following the protocol for tissue extraction. .. Each well contained 1.25 μL Multiplex PCR Kit (Qiagen), the respective primer volumes (Table ) and 1 μL of template DNA.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen). .. Each reaction was carried out with 1 mg MBP-DMI3 protein, 0.2 mM CaCl2 , 0.5 mM bovine CaM, 2 mg 6XHIS-IPD3 as substrate.

    High Throughput Screening Assay:

    Article Title: A Simple, High-Throughput Assay for Fragile X Expanded Alleles Using Triple Repeat Primed PCR and Capillary Electrophoresis
    Article Snippet: DNA was extracted with a manual procedure (see Materials and Methods ) and with the Qiagen BioSprint96 instrument., A–F, shows the electropherograms obtained for six samples extracted using the BioSprint96 instrument. .. DNA was extracted with a manual procedure (see Materials and Methods ) and with the Qiagen BioSprint96 instrument., A–F, shows the electropherograms obtained for six samples extracted using the BioSprint96 instrument.

    Lysis:

    Article Title: Individual Differences in Scanpaths Correspond with Serotonin Transporter Genotype and Behavioral Phenotype in Rhesus Monkeys (Macaca mulatta)
    Article Snippet: Immediately prior to extraction the lysis buffer volumes (with the swab head) were normalized to 1 ml, 25 μl of proteinase K (20 mg/ml) was added to all samples, and they were incubated with agitation at 55°C overnight. .. DNA was isolated from the samples using a protocol modified from the Qiagen BioSprint 96 DNA Blood Kit as follows: The lysate was loaded into two identical 2.2 ml deep well plates.

    Article Title: Identifying avian malaria vectors: sampling methods influence outcomes
    Article Snippet: Thoraxes of each individual mosquito were homogenized using the Qiagen stainless steel 5 mm beads and the Qiagen TissueLyser (Qiagen, Valencia, CA) for 3 min at the maximum speed before the overnight incubation in the lysis buffer provided in the kit. .. DNA was then extracted from thoraxes following the Qiagen BioSprint 96 DNA Tissue Extraction Kit (Qiagen, Valencia, CA) protocol and using the BioSprint 96 instrument (Qiagen, Valencia, CA).

    Article Title: Landscape limits gene flow and drives population structure in Agassiz’s desert tortoise (Gopherus agassizii)
    Article Snippet: Fish and Wildlife Service (TE-06556, TE-17730, BO 8-8-11-F-65R), and complied with the Animal Care and Use Committee of the U.S. Geological Survey and the Animal Research Committee at the University of California, Los Angeles. .. Total genomic DNA was isolated from 50–100 µl of whole blood in 750 µl lysis buffer (50 mM Tris pH 8.0; 50 mM EDTA; 25 mM Sucrose; 100 mM NaCl; 1% SDS) by overnight lysis with proteinase K at 55 °C, followed by robotic extraction using a QIAGEN BioSprint 96 robotic magnetic-particle purification system (Qiagen, Valencia, California, USA) and Invitrogen Dynal bead extraction chemistry (Life Technologies, Carlsbad, California, USA). .. The recovered DNA was quantified using a BioTEK Synergy HT (BioTEK, Vermont, USA) and diluted working stocks to 5 ng/μl in Low TE (10 mMTris-pH 8.0, 0.1 mM EDTA).

    Article Title: Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples, et al. Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples
    Article Snippet: Paragraph title: Lysis and DNA extraction ... The automated DNA extraction was performed using the BioSprint® 96 DNA Blood Kit on a BioSprint® 96 extraction robotic platform (Qiagen).

    Fluorescence In Situ Hybridization:

    Article Title: Co-expression Analysis of Sirtuins and Related Metabolic Biomarkers in Juveniles of Gilthead Sea Bream (Sparus aurata) With Differences in Growth Performance
    Article Snippet: Paragraph title: Fish ... Briefly, DNA was extracted from the fin by using the BioSprint 96 DNA Blood Kit (QIAGEN®) operated by a Biosprint 96 robot.

    Chick Chorioallantoic Membrane Assay:

    Article Title: IPD3 and IPD3L Function Redundantly in Rhizobial and Mycorrhizal Symbioses
    Article Snippet: Purification of MBP-DMI3, HIS-IPD3, and HIS-IPD3L was performed according to the protocols of E8200 (New England Biolab) and of BioSprint96 (Qiagen). .. IPD3 was phosphorylated in vitro by DMI3 as described (Jin et al., ).

    Hood:

    Article Title: Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples, et al. Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples
    Article Snippet: The automated DNA extraction was performed using the BioSprint® 96 DNA Blood Kit on a BioSprint® 96 extraction robotic platform (Qiagen). .. DNA extracts were stored at −28°C until PCR.

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    Qiagen biosprint96 instrument
    Feasibility of using dried blood spots as a sample source the FX Screening assay. One 6-mm punch from each blood spot was extracted on the Qiagen <t>BioSprint96</t> following the standard protocol. A: Female intermediate. B: Female premutation. C: Male full
    Biosprint96 Instrument, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biosprint96 instrument/product/Qiagen
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    biosprint96 instrument - by Bioz Stars, 2019-12
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    83
    Qiagen biosprint96 magnetic bead extractor
    Feasibility of using dried blood spots as a sample source the FX Screening assay. One 6-mm punch from each blood spot was extracted on the Qiagen <t>BioSprint96</t> following the standard protocol. A: Female intermediate. B: Female premutation. C: Male full
    Biosprint96 Magnetic Bead Extractor, supplied by Qiagen, used in various techniques. Bioz Stars score: 83/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biosprint96 magnetic bead extractor/product/Qiagen
    Average 83 stars, based on 3 article reviews
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    biosprint96 magnetic bead extractor - by Bioz Stars, 2019-12
    83/100 stars
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    Feasibility of using dried blood spots as a sample source the FX Screening assay. One 6-mm punch from each blood spot was extracted on the Qiagen BioSprint96 following the standard protocol. A: Female intermediate. B: Female premutation. C: Male full

    Journal:

    Article Title: A Simple, High-Throughput Assay for Fragile X Expanded Alleles Using Triple Repeat Primed PCR and Capillary Electrophoresis

    doi: 10.2353/jmoldx.2010.090229

    Figure Lengend Snippet: Feasibility of using dried blood spots as a sample source the FX Screening assay. One 6-mm punch from each blood spot was extracted on the Qiagen BioSprint96 following the standard protocol. A: Female intermediate. B: Female premutation. C: Male full

    Article Snippet: DNA was extracted with a manual procedure (see Materials and Methods ) and with the Qiagen BioSprint96 instrument., A–F, shows the electropherograms obtained for six samples extracted using the BioSprint96 instrument.

    Techniques: Screening Assay