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Bio-Rad biosilect 125 5 column
Biosilect 125 5 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biosilect 125 5 column/product/Bio-Rad
Average 85 stars, based on 3 article reviews
Price from $9.99 to $1999.99
biosilect 125 5 column - by Bioz Stars, 2020-09
85/100 stars

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size-exclusion chromatography

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High Performance Liquid Chromatography:

Article Title: Cubozoan Venom-Induced Cardiovascular Collapse Is Caused by Hyperkalemia and Prevented by Zinc Gluconate in Mice
Article Snippet: .. Size-exclusion chromatography was performed using a BioSilect 125-5 column (BioRad 125-0060 with BioRad 125-0072) equilibrated with sodium phosphate buffer (50 mM Na2 HPO4 , 50 mM NaH2 PO4 , 150 mM NaCl, pH 6.8) at a rate of 0.5 mL/min using an AKTA Purifier high-pressure liquid chromatography (HPLC) system (GE Biosciences). .. Hemolytic Activity Assay Hemolytic activity assays were performed in 96-well v-bottom microtiter plates by adding washed 2% red blood cells (RBC), prepared from blood of healthy human donors, to serial two-fold dilutions of total venom and incubating at 37°C for 1 hr, or in whole blood time series experiments with the removal of 0.5 mL aliquots at each specific time point, then immediately centrifuged at 1,000 g for 4 min at 4°C.

Size-exclusion Chromatography:

Article Title: Cubozoan Venom-Induced Cardiovascular Collapse Is Caused by Hyperkalemia and Prevented by Zinc Gluconate in Mice
Article Snippet: .. Size-exclusion chromatography was performed using a BioSilect 125-5 column (BioRad 125-0060 with BioRad 125-0072) equilibrated with sodium phosphate buffer (50 mM Na2 HPO4 , 50 mM NaH2 PO4 , 150 mM NaCl, pH 6.8) at a rate of 0.5 mL/min using an AKTA Purifier high-pressure liquid chromatography (HPLC) system (GE Biosciences). .. Hemolytic Activity Assay Hemolytic activity assays were performed in 96-well v-bottom microtiter plates by adding washed 2% red blood cells (RBC), prepared from blood of healthy human donors, to serial two-fold dilutions of total venom and incubating at 37°C for 1 hr, or in whole blood time series experiments with the removal of 0.5 mL aliquots at each specific time point, then immediately centrifuged at 1,000 g for 4 min at 4°C.

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    Bio-Rad biosilect 125 5 column
    Biosilect 125 5 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biosilect 125 5 column/product/Bio-Rad
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biosilect 125 5 column - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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