biologic duoflow maximizer purification system  (Bio-Rad)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 79

    Structured Review

    Bio-Rad biologic duoflow maximizer purification system
    Biologic Duoflow Maximizer Purification System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biologic duoflow maximizer purification system/product/Bio-Rad
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biologic duoflow maximizer purification system - by Bioz Stars, 2020-01
    79/100 stars

    Images

    Related Articles

    Affinity Chromatography:

    Article Title: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface
    Article Snippet: Paragraph title: Purification of Recombinant Common Salivary Protein-1 (Rcsp-1) by Metal Affinity Followed by Con A Lectin Affinity Chromatography ... The secreted rCSP-1 protein was purified at 4 °C by metal chelate chromatography using TALON Superflow metal affinity resin (Clontech, Palo Alto, CA, USA) on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) as described in Ambatipudi et. al. .

    Chromatography:

    Article Title: Quantitative Analysis of Age Specific Variation in the Abundance of Human Female Parotid Salivary Proteins
    Article Snippet: Paragraph title: Large Scale Protein Fractionation of Parotid Saliva by Hydrophobic Charge Interaction Chromatography (HCIC) ... Pooled parotid saliva was subjected to HCIC performed on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) using a 2 ml column with 4-mercaptoethylpyridine (MEP Hypercel) as ligand, which at high concentration adsorbs via hydrophobic interactions without use of high concentration of lyotropic salts 25.

    Article Title: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface
    Article Snippet: After 5 days, the culture medium was harvested and buffer exchanged, using a 10-kDa molecular mass cut-off membrane filter and a pellicon tangential flow system (Millipore, Billerica, MA, USA). .. The secreted rCSP-1 protein was purified at 4 °C by metal chelate chromatography using TALON Superflow metal affinity resin (Clontech, Palo Alto, CA, USA) on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) as described in Ambatipudi et. al. . .. In briefly, the column was equilibrated with 50 mM sodium phosphate and 300 mM sodium chloride pH 8.

    Purification:

    Article Title: Quantitative Analysis of Age Specific Variation in the Abundance of Human Female Parotid Salivary Proteins
    Article Snippet: To normalize the differences between subjects and to reduce individual variation, parotid saliva collected from each of the seven subjects within an age group were pooled (approximately 70ml). .. Pooled parotid saliva was subjected to HCIC performed on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) using a 2 ml column with 4-mercaptoethylpyridine (MEP Hypercel) as ligand, which at high concentration adsorbs via hydrophobic interactions without use of high concentration of lyotropic salts 25. .. Proteins binding to the ligand was carried out at neutral conditions followed by desorption at low pH, thus inducing an ionic charge causing repulsion between the ionizable ligand and bound protein26.

    Article Title: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface
    Article Snippet: After 5 days, the culture medium was harvested and buffer exchanged, using a 10-kDa molecular mass cut-off membrane filter and a pellicon tangential flow system (Millipore, Billerica, MA, USA). .. The secreted rCSP-1 protein was purified at 4 °C by metal chelate chromatography using TALON Superflow metal affinity resin (Clontech, Palo Alto, CA, USA) on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) as described in Ambatipudi et. al. . .. In briefly, the column was equilibrated with 50 mM sodium phosphate and 300 mM sodium chloride pH 8.

    Concentration Assay:

    Article Title: Quantitative Analysis of Age Specific Variation in the Abundance of Human Female Parotid Salivary Proteins
    Article Snippet: To normalize the differences between subjects and to reduce individual variation, parotid saliva collected from each of the seven subjects within an age group were pooled (approximately 70ml). .. Pooled parotid saliva was subjected to HCIC performed on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) using a 2 ml column with 4-mercaptoethylpyridine (MEP Hypercel) as ligand, which at high concentration adsorbs via hydrophobic interactions without use of high concentration of lyotropic salts 25. .. Proteins binding to the ligand was carried out at neutral conditions followed by desorption at low pH, thus inducing an ionic charge causing repulsion between the ionizable ligand and bound protein26.

    Fractionation:

    Article Title: Quantitative Analysis of Age Specific Variation in the Abundance of Human Female Parotid Salivary Proteins
    Article Snippet: Paragraph title: Large Scale Protein Fractionation of Parotid Saliva by Hydrophobic Charge Interaction Chromatography (HCIC) ... Pooled parotid saliva was subjected to HCIC performed on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) using a 2 ml column with 4-mercaptoethylpyridine (MEP Hypercel) as ligand, which at high concentration adsorbs via hydrophobic interactions without use of high concentration of lyotropic salts 25.

    Recombinant:

    Article Title: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface
    Article Snippet: Paragraph title: Purification of Recombinant Common Salivary Protein-1 (Rcsp-1) by Metal Affinity Followed by Con A Lectin Affinity Chromatography ... The secreted rCSP-1 protein was purified at 4 °C by metal chelate chromatography using TALON Superflow metal affinity resin (Clontech, Palo Alto, CA, USA) on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) as described in Ambatipudi et. al. .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 79
    Bio-Rad biologic duoflow maximizer purification system
    Biologic Duoflow Maximizer Purification System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biologic duoflow maximizer purification system/product/Bio-Rad
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biologic duoflow maximizer purification system - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    Image Search Results