biologic duoflow maximizer purification system  (Bio-Rad)

 
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    Structured Review

    Bio-Rad biologic duoflow maximizer purification system
    Biologic Duoflow Maximizer Purification System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biologic duoflow maximizer purification system/product/Bio-Rad
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biologic duoflow maximizer purification system - by Bioz Stars, 2020-08
    93/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Tonoplast-localized Abc2 Transporter Mediates Phytochelatin Accumulation in Vacuoles and Confers Cadmium Tolerance *
    Article Snippet: .. HMWCs in cell extracts were separated by size exclusion chromatography using a Superdex 75 10/300 GL column equilibrated with 20 m m Tris pH 7.7 (GE Healthcare) and a BioLogic DuoFlow FPLC system (Bio-Rad) at flow rate of 1 ml/min. .. S. pombe cells were grown on YES liquid medium to a A 600 nm 1.0 and then exposed to 200 μ m CdCl2 for 20 h. Cells were harvested by centrifugation, washed twice with 20 m m Tris, pH 7.7, and broken with glass beads.

    Article Title: The Proteomes of Human Parotid and Submandibular/Sublingual Gland Salivas Collected as the Ductal Secretions
    Article Snippet: .. Purifications were performed either by gravity flow or by using a Biologic DuoFlow Maximizer purification system (Bio-Rad). .. MS analyses were performed after a single chromatographic fractionation and after multidimensional separations.

    Filtration:

    Article Title: The Crystal Structure and Mechanism of an Unusual Oxidoreductase, GilR, Involved in Gilvocarcin V Biosynthesis *
    Article Snippet: .. GilR was concentrated to ∼200 μl with an Ultracel 30K filter (Millipore) and further purified by gel filtration across a Superose 6 10/300 GL Tricorn high performance column (Amersham Biosciences) using a BioLogic DuoFlow FPLC system (Bio-Rad) and crystallization buffer (20 m m Tris, pH 7.5, 200 m m NaCl). ..

    Chromatography:

    Article Title: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface
    Article Snippet: .. The secreted rCSP-1 protein was purified at 4 °C by metal chelate chromatography using TALON Superflow metal affinity resin (Clontech, Palo Alto, CA, USA) on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) as described in Ambatipudi et. al. . ..

    Article Title: Enzyme replacement for GM1-gangliosidosis: Uptake, lysosomal activation, and cellular disease correction using a novel β-galactosidase:RTB lectin fusion
    Article Snippet: .. Chromatography was conducted using a Biologic Duoflow FPLC system (Bio-Rad). .. For purification of RTB:β-gal and β-gal:RTB, α-lactose agarose gel was conditioned with 20 column volumes (CV) of 200 mM Tris, 150 mM NaCl, pH 7.8.

    Size-exclusion Chromatography:

    Article Title: Tonoplast-localized Abc2 Transporter Mediates Phytochelatin Accumulation in Vacuoles and Confers Cadmium Tolerance *
    Article Snippet: .. HMWCs in cell extracts were separated by size exclusion chromatography using a Superdex 75 10/300 GL column equilibrated with 20 m m Tris pH 7.7 (GE Healthcare) and a BioLogic DuoFlow FPLC system (Bio-Rad) at flow rate of 1 ml/min. .. S. pombe cells were grown on YES liquid medium to a A 600 nm 1.0 and then exposed to 200 μ m CdCl2 for 20 h. Cells were harvested by centrifugation, washed twice with 20 m m Tris, pH 7.7, and broken with glass beads.

    Article Title: Templated Construction of a Zn-Selective Protein Dimerization Motif
    Article Snippet: .. Size exclusion chromatography was performed on a Biologic DuoFlow FPLC system (Bio-Rad) equipped with a UV detector set to monitor absorbance at 280 nm. .. For each run, 100 μl of 25 μM BMOE RIDC12 in 20 mM TRIS (pH 7) buffer with 50mM NaCl and either 50 μM ZnCl2 or 5 mM EDTA were injected on an analytical SEC column packed with Pall ACA54 resin (fractionation range = 5–70 kDa).

    Purification:

    Article Title: Oculocutaneous Albinism Type 1: Link between Mutations, Tyrosinase Conformational Stability, and Enzymatic Activity
    Article Snippet: .. The purification was performed using a Bio-Logic Duo-Flow Maximizer workstation (Bio-Rad, CA). .. First, the macrofiltered supernatant was loaded onto a 5 ml His-Trap FF Crude IMAC column (GE Healthcare, NJ) equilibrated preliminary with Buffer A, containing the detergent, and eluted at a flow rate of 0.5 ml/min.

    Article Title: Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface
    Article Snippet: .. The secreted rCSP-1 protein was purified at 4 °C by metal chelate chromatography using TALON Superflow metal affinity resin (Clontech, Palo Alto, CA, USA) on a Biologic DuoFlow Maximizer purification system (Bio-Rad, Hercules, CA, USA) as described in Ambatipudi et. al. . ..

    Article Title: The Proteomes of Human Parotid and Submandibular/Sublingual Gland Salivas Collected as the Ductal Secretions
    Article Snippet: .. Purifications were performed either by gravity flow or by using a Biologic DuoFlow Maximizer purification system (Bio-Rad). .. MS analyses were performed after a single chromatographic fractionation and after multidimensional separations.

    Article Title: The Crystal Structure and Mechanism of an Unusual Oxidoreductase, GilR, Involved in Gilvocarcin V Biosynthesis *
    Article Snippet: .. GilR was concentrated to ∼200 μl with an Ultracel 30K filter (Millipore) and further purified by gel filtration across a Superose 6 10/300 GL Tricorn high performance column (Amersham Biosciences) using a BioLogic DuoFlow FPLC system (Bio-Rad) and crystallization buffer (20 m m Tris, pH 7.5, 200 m m NaCl). ..

    Fast Protein Liquid Chromatography:

    Article Title: Tonoplast-localized Abc2 Transporter Mediates Phytochelatin Accumulation in Vacuoles and Confers Cadmium Tolerance *
    Article Snippet: .. HMWCs in cell extracts were separated by size exclusion chromatography using a Superdex 75 10/300 GL column equilibrated with 20 m m Tris pH 7.7 (GE Healthcare) and a BioLogic DuoFlow FPLC system (Bio-Rad) at flow rate of 1 ml/min. .. S. pombe cells were grown on YES liquid medium to a A 600 nm 1.0 and then exposed to 200 μ m CdCl2 for 20 h. Cells were harvested by centrifugation, washed twice with 20 m m Tris, pH 7.7, and broken with glass beads.

    Article Title: Autoimmune-Mediated Reduction of High-Density Lipoprotein-Cholesterol and Paraoxonase-1 Activity in SLE-Prone gld Mice
    Article Snippet: .. Plasma (200μl pooled from 3 mice) was fractionated using a Superose-6 column (Amersham Biosciences) on a Biologic DuoFlow FPLC system (Bio-Rad) as previously described ( ). .. PON1 activity was measured using 1mM Paraoxon (Supelco) as substrate (1 unit of PON1 activity=1 nmole of 4-nitrophenol/minute).

    Article Title: Templated Construction of a Zn-Selective Protein Dimerization Motif
    Article Snippet: .. Size exclusion chromatography was performed on a Biologic DuoFlow FPLC system (Bio-Rad) equipped with a UV detector set to monitor absorbance at 280 nm. .. For each run, 100 μl of 25 μM BMOE RIDC12 in 20 mM TRIS (pH 7) buffer with 50mM NaCl and either 50 μM ZnCl2 or 5 mM EDTA were injected on an analytical SEC column packed with Pall ACA54 resin (fractionation range = 5–70 kDa).

    Article Title: Enzyme replacement for GM1-gangliosidosis: Uptake, lysosomal activation, and cellular disease correction using a novel β-galactosidase:RTB lectin fusion
    Article Snippet: .. Chromatography was conducted using a Biologic Duoflow FPLC system (Bio-Rad). .. For purification of RTB:β-gal and β-gal:RTB, α-lactose agarose gel was conditioned with 20 column volumes (CV) of 200 mM Tris, 150 mM NaCl, pH 7.8.

    Article Title: The Crystal Structure and Mechanism of an Unusual Oxidoreductase, GilR, Involved in Gilvocarcin V Biosynthesis *
    Article Snippet: .. GilR was concentrated to ∼200 μl with an Ultracel 30K filter (Millipore) and further purified by gel filtration across a Superose 6 10/300 GL Tricorn high performance column (Amersham Biosciences) using a BioLogic DuoFlow FPLC system (Bio-Rad) and crystallization buffer (20 m m Tris, pH 7.5, 200 m m NaCl). ..

    Crystallization Assay:

    Article Title: The Crystal Structure and Mechanism of an Unusual Oxidoreductase, GilR, Involved in Gilvocarcin V Biosynthesis *
    Article Snippet: .. GilR was concentrated to ∼200 μl with an Ultracel 30K filter (Millipore) and further purified by gel filtration across a Superose 6 10/300 GL Tricorn high performance column (Amersham Biosciences) using a BioLogic DuoFlow FPLC system (Bio-Rad) and crystallization buffer (20 m m Tris, pH 7.5, 200 m m NaCl). ..

    Mouse Assay:

    Article Title: Autoimmune-Mediated Reduction of High-Density Lipoprotein-Cholesterol and Paraoxonase-1 Activity in SLE-Prone gld Mice
    Article Snippet: .. Plasma (200μl pooled from 3 mice) was fractionated using a Superose-6 column (Amersham Biosciences) on a Biologic DuoFlow FPLC system (Bio-Rad) as previously described ( ). .. PON1 activity was measured using 1mM Paraoxon (Supelco) as substrate (1 unit of PON1 activity=1 nmole of 4-nitrophenol/minute).

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  • About
  • News
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  • Team
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  • Contact
  • Bioz Stars
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  • 93
    Bio-Rad biologic duoflow maximizer purification system
    Biologic Duoflow Maximizer Purification System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biologic duoflow maximizer purification system/product/Bio-Rad
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biologic duoflow maximizer purification system - by Bioz Stars, 2020-08
    93/100 stars
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