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1) Product Images from "Transcriptome analysis of Streptococcus gordonii Challis DL1 indicates a role for the biofilm-associated fruRBA operon in response to Candida albicans"
Article Title: Transcriptome analysis of Streptococcus gordonii Challis DL1 indicates a role for the biofilm-associated fruRBA operon in response to Candida albicans
Journal: Molecular oral microbiology
Figure Legend Snippet: CLSM images of dual-species C. albicans SC5314- S. gordonii biofilms formed on saliva-coated glass cover slips in YPT-G medium. Biofilms were grown as described in Methods for 6 h at 37°C and fluorescently stained with FITC. For each of the panels (A–D) a series of x-y sections were collected to generate 3D reconstructions of representative CLSM images with Volocity® software. Heights were measured across ten CLSM image stacks from two independent experiments ± SD. Panels: (a), C. albicans-S.gordonii DL1, height 81.4 ± 7.50 μm; (b), C. albcians-S. gordonii Δ fruRBA , height 113 ± 10.4 μm*; (c), C. albicans-S. gordonii Δ fruRBA /p fruRBA, height 70.6 ± 6.40 μm; (d), C. albicans , height 86.8 ± 11.6. * P
Techniques Used: Confocal Laser Scanning Microscopy, Staining, Software
Figure Legend Snippet: Images of single- or dual-species biofilm formation in YPT-G medium by S. gordonii cells on saliva-coated cover slips with or without attached C. albicans SC5314 cells. Representative light microscopic fields of crystal violet-stained biofilms are shown. The middle panels show S. gordonii biofilms of the parent (top), Δ fruRBA mutant (middle) and fruRBA complemented (bottom) strains (DL1, UB2683, and UB2683/p49M fruRBA , respectively) grown from cells that bound during a 30-min incubation period to substrata with attached C. albicans cells. The flanking panels show corresponding monospecies biofilms of S. gordonii (left panels) grown from cells that bound saliva-coated substrata over 30-min incubation, and C. albicans biofilm (right panel) grown from cells that attached during 1-h incubation. Magnification Bar = 50 μm.
Techniques Used: Staining, Mutagenesis, Incubation
Figure Legend Snippet: Biofilms formed on saliva-coated glass cover slips. Sixteen hour biofilms of S. gordonii DL1 or Δ fruRBA grown in TY-G or TY-F media (top panels), and in YPT-G or YPT-F media (bottom panels). Two representative views of transmitted light microscopy fields of crystal violet -stained biofilms formed in each medium are shown for each strain.
Techniques Used: Light Microscopy, Staining