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Johnson & Johnson biofilms
An overview of the high-throughput protocol for metal susceptibility testing using the MBEC assay . (A) Frozen stocks of bacteria were streaked out on the appropriate agar medium to obtain a first- and a subsequent second-subculture. (B) Colonies were collected from second-subcultures and suspended in broth medium to a 1.0 McFarland Standard. (C) This suspension was diluted 30-fold in broth, and the 1 in 30 dilution was used to inoculate the MBEC assay. (D) The inoculated device was placed on a rocking table in an incubator. (E) Serial dilutions of metal cations and oxyanions were set up along length of a microtiter plate along (the challenge plate). (F) The <t>biofilms</t> were rinsed to remove loosely adherent planktonic bacteria. (G) The first peg from each row was removed. These pegs were used to verify growth of the biofilms on the pegs. The peg lid was then inserted into the challenge plate. (H) During exposure, metals diffuse into the biofilm while planktonic cells are shed from the surface of the biofilm. Sloughed cells serve as the inoculum for planktonic MIC and MBC determinations. (I) The exposed biofilms were rinsed twice and the peg lid was inserted into fresh recovery medium containing the appropriate neutralizing agent (the recovery plate). The biofilms were disrupted into the recovery medium by sonciation on a water table sonicator. (J) Aliquots of planktonic cultures were transferred from the challenge plate to a microtiter plate containing the appropriate neutralizing agents (the neutralizing plate). (K) An aliquot from the recovery and neutralizing plates were spotted onto rich agar media. (L) MIC values are determined by reading the optical density at 650 nm (OD 650 ) of the challenge plate after the desired period of incubation using a microtiter plate reader. Spot plates were qualitatively scored for growth to obtain MBC and MBEC values. MBEC values were redundantly determined by determining the A 650 of the recovery plates after incubation.
Biofilms, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "High-throughput metal susceptibility testing of microbial biofilms"

Article Title: High-throughput metal susceptibility testing of microbial biofilms

Journal: BMC Microbiology

doi: 10.1186/1471-2180-5-53

An overview of the high-throughput protocol for metal susceptibility testing using the MBEC assay . (A) Frozen stocks of bacteria were streaked out on the appropriate agar medium to obtain a first- and a subsequent second-subculture. (B) Colonies were collected from second-subcultures and suspended in broth medium to a 1.0 McFarland Standard. (C) This suspension was diluted 30-fold in broth, and the 1 in 30 dilution was used to inoculate the MBEC assay. (D) The inoculated device was placed on a rocking table in an incubator. (E) Serial dilutions of metal cations and oxyanions were set up along length of a microtiter plate along (the challenge plate). (F) The biofilms were rinsed to remove loosely adherent planktonic bacteria. (G) The first peg from each row was removed. These pegs were used to verify growth of the biofilms on the pegs. The peg lid was then inserted into the challenge plate. (H) During exposure, metals diffuse into the biofilm while planktonic cells are shed from the surface of the biofilm. Sloughed cells serve as the inoculum for planktonic MIC and MBC determinations. (I) The exposed biofilms were rinsed twice and the peg lid was inserted into fresh recovery medium containing the appropriate neutralizing agent (the recovery plate). The biofilms were disrupted into the recovery medium by sonciation on a water table sonicator. (J) Aliquots of planktonic cultures were transferred from the challenge plate to a microtiter plate containing the appropriate neutralizing agents (the neutralizing plate). (K) An aliquot from the recovery and neutralizing plates were spotted onto rich agar media. (L) MIC values are determined by reading the optical density at 650 nm (OD 650 ) of the challenge plate after the desired period of incubation using a microtiter plate reader. Spot plates were qualitatively scored for growth to obtain MBC and MBEC values. MBEC values were redundantly determined by determining the A 650 of the recovery plates after incubation.
Figure Legend Snippet: An overview of the high-throughput protocol for metal susceptibility testing using the MBEC assay . (A) Frozen stocks of bacteria were streaked out on the appropriate agar medium to obtain a first- and a subsequent second-subculture. (B) Colonies were collected from second-subcultures and suspended in broth medium to a 1.0 McFarland Standard. (C) This suspension was diluted 30-fold in broth, and the 1 in 30 dilution was used to inoculate the MBEC assay. (D) The inoculated device was placed on a rocking table in an incubator. (E) Serial dilutions of metal cations and oxyanions were set up along length of a microtiter plate along (the challenge plate). (F) The biofilms were rinsed to remove loosely adherent planktonic bacteria. (G) The first peg from each row was removed. These pegs were used to verify growth of the biofilms on the pegs. The peg lid was then inserted into the challenge plate. (H) During exposure, metals diffuse into the biofilm while planktonic cells are shed from the surface of the biofilm. Sloughed cells serve as the inoculum for planktonic MIC and MBC determinations. (I) The exposed biofilms were rinsed twice and the peg lid was inserted into fresh recovery medium containing the appropriate neutralizing agent (the recovery plate). The biofilms were disrupted into the recovery medium by sonciation on a water table sonicator. (J) Aliquots of planktonic cultures were transferred from the challenge plate to a microtiter plate containing the appropriate neutralizing agents (the neutralizing plate). (K) An aliquot from the recovery and neutralizing plates were spotted onto rich agar media. (L) MIC values are determined by reading the optical density at 650 nm (OD 650 ) of the challenge plate after the desired period of incubation using a microtiter plate reader. Spot plates were qualitatively scored for growth to obtain MBC and MBEC values. MBEC values were redundantly determined by determining the A 650 of the recovery plates after incubation.

Techniques Used: High Throughput Screening Assay, Incubation

Growth of bacterial biofilms in the MBEC assay . (A) Mean cell density of Pseudomonas aeruginosa ATCC 27853 biofilms on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.842 using one-way ANOVA). (B) SEM photomicrograph of a P. aeruginosa biofilm on the peg surface. (C) Mean cell density of Escherichia coli TG1 on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.274 using one-way ANOVA). (D) SEM photomicrograph of an E. coli biofilm on the peg surface. The bar represents 5 μm.
Figure Legend Snippet: Growth of bacterial biofilms in the MBEC assay . (A) Mean cell density of Pseudomonas aeruginosa ATCC 27853 biofilms on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.842 using one-way ANOVA). (B) SEM photomicrograph of a P. aeruginosa biofilm on the peg surface. (C) Mean cell density of Escherichia coli TG1 on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.274 using one-way ANOVA). (D) SEM photomicrograph of an E. coli biofilm on the peg surface. The bar represents 5 μm.

Techniques Used: Standard Deviation

2) Product Images from "Molecular characterization, biofilm analysis and experimental biofouling study of Fusarium isolates from recent cases of fungal keratitis in New York State"

Article Title: Molecular characterization, biofilm analysis and experimental biofouling study of Fusarium isolates from recent cases of fungal keratitis in New York State

Journal: BMC Ophthalmology

doi: 10.1186/1471-2415-7-1

Scanning electron micrographs of biofilm formation . (a) F. solani (238-06) incubated with fresh ACUVUE lens and balanced salt solution for 48 h at 25°C on a rotator (180 rpm). (b) Close-up of the same isolate showing hyphal attachment to the lens surface (c) Disappearance of biofilm when preparation from (a) was treated with MoistureLoc for 4 h.
Figure Legend Snippet: Scanning electron micrographs of biofilm formation . (a) F. solani (238-06) incubated with fresh ACUVUE lens and balanced salt solution for 48 h at 25°C on a rotator (180 rpm). (b) Close-up of the same isolate showing hyphal attachment to the lens surface (c) Disappearance of biofilm when preparation from (a) was treated with MoistureLoc for 4 h.

Techniques Used: Incubation

3) Product Images from "Copper and Quaternary Ammonium Cations Exert Synergistic Bactericidal and Antibiofilm Activity against Pseudomonas aeruginosa "

Article Title: Copper and Quaternary Ammonium Cations Exert Synergistic Bactericidal and Antibiofilm Activity against Pseudomonas aeruginosa

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.00203-08

Combinations of Cu 2+ with other QACs show synergistic killing of P. aeruginosa ATCC 15442 biofilms. Viable cell counts were determined after exposure of biofilms to combinations of Cu 2+ and benzalkonium chloride (a), cetylpyridinium chloride
Figure Legend Snippet: Combinations of Cu 2+ with other QACs show synergistic killing of P. aeruginosa ATCC 15442 biofilms. Viable cell counts were determined after exposure of biofilms to combinations of Cu 2+ and benzalkonium chloride (a), cetylpyridinium chloride

Techniques Used:

Live/Dead BacLight staining of P. aeruginosa ATCC 27853 biofilms exposed to Cu 2+ and Polycide indicates that these agents are bactericidal. In these pictures, green cells are live bacteria and red cells are dead bacteria. (a) Growth controls.
Figure Legend Snippet: Live/Dead BacLight staining of P. aeruginosa ATCC 27853 biofilms exposed to Cu 2+ and Polycide indicates that these agents are bactericidal. In these pictures, green cells are live bacteria and red cells are dead bacteria. (a) Growth controls.

Techniques Used: Staining

High-throughput screening may be used to identify synergistic antimicrobial interactions that kill microbial biofilms. Starting from cryogenic stocks, the desired bacterial strain was streaked out twice on TSA (a), and colonies from the second subcultures
Figure Legend Snippet: High-throughput screening may be used to identify synergistic antimicrobial interactions that kill microbial biofilms. Starting from cryogenic stocks, the desired bacterial strain was streaked out twice on TSA (a), and colonies from the second subcultures

Techniques Used: High Throughput Screening Assay

P. aeruginosa ATCC 15442 biofilms were killed time dependently by combinations of Cu 2+ and Polycide. Viable cell counts were determined after exposure of biofilms to combinations of Cu 2+ and Polycide in ddH 2 O for 10 min (a) or 30 min (b)
Figure Legend Snippet: P. aeruginosa ATCC 15442 biofilms were killed time dependently by combinations of Cu 2+ and Polycide. Viable cell counts were determined after exposure of biofilms to combinations of Cu 2+ and Polycide in ddH 2 O for 10 min (a) or 30 min (b)

Techniques Used:

Killing of Escherichia coli (a), Pseudomonas fluorescens (b), Salmonella enterica serovar Cholerasuis (c), and Staphylococcus aureus (d) biofilms by combinations of Cu 2+ and Polycide. These data are for 24 h of exposure in dilute organics, and
Figure Legend Snippet: Killing of Escherichia coli (a), Pseudomonas fluorescens (b), Salmonella enterica serovar Cholerasuis (c), and Staphylococcus aureus (d) biofilms by combinations of Cu 2+ and Polycide. These data are for 24 h of exposure in dilute organics, and

Techniques Used:

Related Articles

Incubation:

Article Title: Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles
Article Snippet: .. C. albicans cells were grown on Sabouraud dextrose agar (Sigma Aldrich) at 35 °C for 24 h. Then the cells were suspended into a saline solution with or without PLGA NPs and incubated for 3 h. The biofilm was grown on silicone–hydrogel contact lenses (Day Acuvue Moist, Johnson & Johnson, New Brunswick, NJ, USA). .. After 24 and 48 h of incubation at 37 °C, PLGA NPs were added and incubated for 3 h. Before the observation, the contact lenses were subjected to three washes in physiological solution to remove the non-adherent PLGA NPs from the biofilm.

other:

Article Title: Molecular characterization, biofilm analysis and experimental biofouling study of Fusarium isolates from recent cases of fungal keratitis in New York State
Article Snippet: Therefore, the ability of the Fusarium isolates to form biofilms in the laboratory was tested with etafilcon A contact lenses (ACUVUE® , Johnson & Johnson Vision Care, Inc.) using a published procedure [ ].

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    Johnson & Johnson calgary biofilm device
    Stationary-phase planktonic cell populations of Δ yafQ and isogenic parental strain E. coli K-12 BW25113 had similar numbers of cells surviving exposure to cefazolin and tobramycin. Mean log killing was calculated after the <t>biofilms</t> had been exposed
    Calgary Biofilm Device, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calgary biofilm device/product/Johnson & Johnson
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calgary biofilm device - by Bioz Stars, 2020-05
    89/100 stars
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    92
    Johnson & Johnson biofilms
    An overview of the high-throughput protocol for metal susceptibility testing using the MBEC assay . (A) Frozen stocks of bacteria were streaked out on the appropriate agar medium to obtain a first- and a subsequent second-subculture. (B) Colonies were collected from second-subcultures and suspended in broth medium to a 1.0 McFarland Standard. (C) This suspension was diluted 30-fold in broth, and the 1 in 30 dilution was used to inoculate the MBEC assay. (D) The inoculated device was placed on a rocking table in an incubator. (E) Serial dilutions of metal cations and oxyanions were set up along length of a microtiter plate along (the challenge plate). (F) The <t>biofilms</t> were rinsed to remove loosely adherent planktonic bacteria. (G) The first peg from each row was removed. These pegs were used to verify growth of the biofilms on the pegs. The peg lid was then inserted into the challenge plate. (H) During exposure, metals diffuse into the biofilm while planktonic cells are shed from the surface of the biofilm. Sloughed cells serve as the inoculum for planktonic MIC and MBC determinations. (I) The exposed biofilms were rinsed twice and the peg lid was inserted into fresh recovery medium containing the appropriate neutralizing agent (the recovery plate). The biofilms were disrupted into the recovery medium by sonciation on a water table sonicator. (J) Aliquots of planktonic cultures were transferred from the challenge plate to a microtiter plate containing the appropriate neutralizing agents (the neutralizing plate). (K) An aliquot from the recovery and neutralizing plates were spotted onto rich agar media. (L) MIC values are determined by reading the optical density at 650 nm (OD 650 ) of the challenge plate after the desired period of incubation using a microtiter plate reader. Spot plates were qualitatively scored for growth to obtain MBC and MBEC values. MBEC values were redundantly determined by determining the A 650 of the recovery plates after incubation.
    Biofilms, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biofilms/product/Johnson & Johnson
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biofilms - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Stationary-phase planktonic cell populations of Δ yafQ and isogenic parental strain E. coli K-12 BW25113 had similar numbers of cells surviving exposure to cefazolin and tobramycin. Mean log killing was calculated after the biofilms had been exposed

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The Chromosomal Toxin Gene yafQ Is a Determinant of Multidrug Tolerance for Escherichia coli Growing in a Biofilm ▿

    doi: 10.1128/AAC.00043-09

    Figure Lengend Snippet: Stationary-phase planktonic cell populations of Δ yafQ and isogenic parental strain E. coli K-12 BW25113 had similar numbers of cells surviving exposure to cefazolin and tobramycin. Mean log killing was calculated after the biofilms had been exposed

    Article Snippet: These criteria have been set by using an empirical approach to assess the normal variation in high-throughput cell viability data generated with the Calgary biofilm device (J. J. Harrison and H. Ceri, unpublished data).

    Techniques:

    Overexpression of yafQ from a high-copy-number plasmid had no effect on the number of cells in E. coli K-12 BW25113 biofilm populations surviving exposure to doxycycline or rifampin. Mean log killing was calculated from the viable cell counts after the

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The Chromosomal Toxin Gene yafQ Is a Determinant of Multidrug Tolerance for Escherichia coli Growing in a Biofilm ▿

    doi: 10.1128/AAC.00043-09

    Figure Lengend Snippet: Overexpression of yafQ from a high-copy-number plasmid had no effect on the number of cells in E. coli K-12 BW25113 biofilm populations surviving exposure to doxycycline or rifampin. Mean log killing was calculated from the viable cell counts after the

    Article Snippet: These criteria have been set by using an empirical approach to assess the normal variation in high-throughput cell viability data generated with the Calgary biofilm device (J. J. Harrison and H. Ceri, unpublished data).

    Techniques: Over Expression, Plasmid Preparation

    Biofilm populations of the Δ yafQ strain have decreased numbers of cells surviving exposure to cefazolin and tobramycin compared to the numbers of parental E. coli K-12 BW25113 cells. Mean log killing was calculated after the biofilms had been

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The Chromosomal Toxin Gene yafQ Is a Determinant of Multidrug Tolerance for Escherichia coli Growing in a Biofilm ▿

    doi: 10.1128/AAC.00043-09

    Figure Lengend Snippet: Biofilm populations of the Δ yafQ strain have decreased numbers of cells surviving exposure to cefazolin and tobramycin compared to the numbers of parental E. coli K-12 BW25113 cells. Mean log killing was calculated after the biofilms had been

    Article Snippet: These criteria have been set by using an empirical approach to assess the normal variation in high-throughput cell viability data generated with the Calgary biofilm device (J. J. Harrison and H. Ceri, unpublished data).

    Techniques:

    Biofilm formation by wild-type E. coli K-12 BW25113 is similar to that of its isogenic Δ yafQ mutant. (a) Biofilm mean viable cell counts and standard deviations were comparable for the two strains, and this assessment was based on the indicated

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The Chromosomal Toxin Gene yafQ Is a Determinant of Multidrug Tolerance for Escherichia coli Growing in a Biofilm ▿

    doi: 10.1128/AAC.00043-09

    Figure Lengend Snippet: Biofilm formation by wild-type E. coli K-12 BW25113 is similar to that of its isogenic Δ yafQ mutant. (a) Biofilm mean viable cell counts and standard deviations were comparable for the two strains, and this assessment was based on the indicated

    Article Snippet: These criteria have been set by using an empirical approach to assess the normal variation in high-throughput cell viability data generated with the Calgary biofilm device (J. J. Harrison and H. Ceri, unpublished data).

    Techniques: Mutagenesis

    Biofilm populations of the Δ yafQ strain and isogenic parental strain E. coli K-12 BW25113 had similar numbers of cells surviving exposure to doxycycline and rifampin. Mean log killing was calculated from the viable cell counts after the biofilms

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The Chromosomal Toxin Gene yafQ Is a Determinant of Multidrug Tolerance for Escherichia coli Growing in a Biofilm ▿

    doi: 10.1128/AAC.00043-09

    Figure Lengend Snippet: Biofilm populations of the Δ yafQ strain and isogenic parental strain E. coli K-12 BW25113 had similar numbers of cells surviving exposure to doxycycline and rifampin. Mean log killing was calculated from the viable cell counts after the biofilms

    Article Snippet: These criteria have been set by using an empirical approach to assess the normal variation in high-throughput cell viability data generated with the Calgary biofilm device (J. J. Harrison and H. Ceri, unpublished data).

    Techniques:

    Overexpression of yafQ from a high-copy-number plasmid increased the number of cells in E. coli K-12 BW25113 biofilm populations surviving exposure to bactericidal concentrations of cefazolin and tobramycin. Mean log killing was calculated from the viable

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The Chromosomal Toxin Gene yafQ Is a Determinant of Multidrug Tolerance for Escherichia coli Growing in a Biofilm ▿

    doi: 10.1128/AAC.00043-09

    Figure Lengend Snippet: Overexpression of yafQ from a high-copy-number plasmid increased the number of cells in E. coli K-12 BW25113 biofilm populations surviving exposure to bactericidal concentrations of cefazolin and tobramycin. Mean log killing was calculated from the viable

    Article Snippet: These criteria have been set by using an empirical approach to assess the normal variation in high-throughput cell viability data generated with the Calgary biofilm device (J. J. Harrison and H. Ceri, unpublished data).

    Techniques: Over Expression, Plasmid Preparation

    An overview of the high-throughput protocol for metal susceptibility testing using the MBEC assay . (A) Frozen stocks of bacteria were streaked out on the appropriate agar medium to obtain a first- and a subsequent second-subculture. (B) Colonies were collected from second-subcultures and suspended in broth medium to a 1.0 McFarland Standard. (C) This suspension was diluted 30-fold in broth, and the 1 in 30 dilution was used to inoculate the MBEC assay. (D) The inoculated device was placed on a rocking table in an incubator. (E) Serial dilutions of metal cations and oxyanions were set up along length of a microtiter plate along (the challenge plate). (F) The biofilms were rinsed to remove loosely adherent planktonic bacteria. (G) The first peg from each row was removed. These pegs were used to verify growth of the biofilms on the pegs. The peg lid was then inserted into the challenge plate. (H) During exposure, metals diffuse into the biofilm while planktonic cells are shed from the surface of the biofilm. Sloughed cells serve as the inoculum for planktonic MIC and MBC determinations. (I) The exposed biofilms were rinsed twice and the peg lid was inserted into fresh recovery medium containing the appropriate neutralizing agent (the recovery plate). The biofilms were disrupted into the recovery medium by sonciation on a water table sonicator. (J) Aliquots of planktonic cultures were transferred from the challenge plate to a microtiter plate containing the appropriate neutralizing agents (the neutralizing plate). (K) An aliquot from the recovery and neutralizing plates were spotted onto rich agar media. (L) MIC values are determined by reading the optical density at 650 nm (OD 650 ) of the challenge plate after the desired period of incubation using a microtiter plate reader. Spot plates were qualitatively scored for growth to obtain MBC and MBEC values. MBEC values were redundantly determined by determining the A 650 of the recovery plates after incubation.

    Journal: BMC Microbiology

    Article Title: High-throughput metal susceptibility testing of microbial biofilms

    doi: 10.1186/1471-2180-5-53

    Figure Lengend Snippet: An overview of the high-throughput protocol for metal susceptibility testing using the MBEC assay . (A) Frozen stocks of bacteria were streaked out on the appropriate agar medium to obtain a first- and a subsequent second-subculture. (B) Colonies were collected from second-subcultures and suspended in broth medium to a 1.0 McFarland Standard. (C) This suspension was diluted 30-fold in broth, and the 1 in 30 dilution was used to inoculate the MBEC assay. (D) The inoculated device was placed on a rocking table in an incubator. (E) Serial dilutions of metal cations and oxyanions were set up along length of a microtiter plate along (the challenge plate). (F) The biofilms were rinsed to remove loosely adherent planktonic bacteria. (G) The first peg from each row was removed. These pegs were used to verify growth of the biofilms on the pegs. The peg lid was then inserted into the challenge plate. (H) During exposure, metals diffuse into the biofilm while planktonic cells are shed from the surface of the biofilm. Sloughed cells serve as the inoculum for planktonic MIC and MBC determinations. (I) The exposed biofilms were rinsed twice and the peg lid was inserted into fresh recovery medium containing the appropriate neutralizing agent (the recovery plate). The biofilms were disrupted into the recovery medium by sonciation on a water table sonicator. (J) Aliquots of planktonic cultures were transferred from the challenge plate to a microtiter plate containing the appropriate neutralizing agents (the neutralizing plate). (K) An aliquot from the recovery and neutralizing plates were spotted onto rich agar media. (L) MIC values are determined by reading the optical density at 650 nm (OD 650 ) of the challenge plate after the desired period of incubation using a microtiter plate reader. Spot plates were qualitatively scored for growth to obtain MBC and MBEC values. MBEC values were redundantly determined by determining the A 650 of the recovery plates after incubation.

    Article Snippet: In general, biofilms formed using a trough have a 5- to 10-fold greater cell density than those formed using the microtiter plate format (J.J. Harrison, H. Ceri and C. Stremick, unpublished data).

    Techniques: High Throughput Screening Assay, Incubation

    Growth of bacterial biofilms in the MBEC assay . (A) Mean cell density of Pseudomonas aeruginosa ATCC 27853 biofilms on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.842 using one-way ANOVA). (B) SEM photomicrograph of a P. aeruginosa biofilm on the peg surface. (C) Mean cell density of Escherichia coli TG1 on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.274 using one-way ANOVA). (D) SEM photomicrograph of an E. coli biofilm on the peg surface. The bar represents 5 μm.

    Journal: BMC Microbiology

    Article Title: High-throughput metal susceptibility testing of microbial biofilms

    doi: 10.1186/1471-2180-5-53

    Figure Lengend Snippet: Growth of bacterial biofilms in the MBEC assay . (A) Mean cell density of Pseudomonas aeruginosa ATCC 27853 biofilms on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.842 using one-way ANOVA). (B) SEM photomicrograph of a P. aeruginosa biofilm on the peg surface. (C) Mean cell density of Escherichia coli TG1 on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.274 using one-way ANOVA). (D) SEM photomicrograph of an E. coli biofilm on the peg surface. The bar represents 5 μm.

    Article Snippet: In general, biofilms formed using a trough have a 5- to 10-fold greater cell density than those formed using the microtiter plate format (J.J. Harrison, H. Ceri and C. Stremick, unpublished data).

    Techniques: Standard Deviation