Structured Review

Agilent technologies bioanalyzer dna high sensitivity chip
Bioanalyzer Dna High Sensitivity Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bioanalyzer dna high sensitivity chip/product/Agilent technologies
Average 93 stars, based on 18 article reviews
Price from $9.99 to $1999.99
bioanalyzer dna high sensitivity chip - by Bioz Stars, 2020-05
93/100 stars

Images

Related Articles

Polymerase Chain Reaction:

Article Title: Metabolic potential of a single cell belonging to one of the most abundant lineages in freshwater bacterioplankton
Article Snippet: .. The normalized library was amplified by PCR for 12 cycles, gel-purified and QC assessed on a Bioanalyzer DNA High Sensitivity chip (Agilent), and then sequenced using an Illumina GAIIx sequencer generating 7.4 Gb (96.8 M reads, 2 × 76 bp). .. For 454 pyrosequencing, a 4-kb paired-end library was constructed and sequenced generating 79.5 Mb (260 428 reads).

Real-time Polymerase Chain Reaction:

Article Title: A limited capacity for microglial repopulation in the adult brain
Article Snippet: .. The resulting libraries were validated by qPCR and sized by Agilent Bioanalyzer DNA high sensitivity chip. ..

Article Title: Defining the Independence of the Liver Circadian Clock
Article Snippet: .. The resulting libraries were validated by qPCR and sized by Agilent Bioanalyzer DNA high sensitivity chip. .. The concentration for denaturation was 2nM and the final concentration for clustering was 200pM.

Article Title: The RhoJ-BAD signaling network: An Achilles’ heel for BRAF mutant melanomas
Article Snippet: .. The resulting libraries were validated by qPCR and sized by Agilent Bioanalyzer DNA high sensitivity chip. ..

Amplification:

Article Title: Metabolic potential of a single cell belonging to one of the most abundant lineages in freshwater bacterioplankton
Article Snippet: .. The normalized library was amplified by PCR for 12 cycles, gel-purified and QC assessed on a Bioanalyzer DNA High Sensitivity chip (Agilent), and then sequenced using an Illumina GAIIx sequencer generating 7.4 Gb (96.8 M reads, 2 × 76 bp). .. For 454 pyrosequencing, a 4-kb paired-end library was constructed and sequenced generating 79.5 Mb (260 428 reads).

Chromatin Immunoprecipitation:

Article Title: Metabolic potential of a single cell belonging to one of the most abundant lineages in freshwater bacterioplankton
Article Snippet: .. The normalized library was amplified by PCR for 12 cycles, gel-purified and QC assessed on a Bioanalyzer DNA High Sensitivity chip (Agilent), and then sequenced using an Illumina GAIIx sequencer generating 7.4 Gb (96.8 M reads, 2 × 76 bp). .. For 454 pyrosequencing, a 4-kb paired-end library was constructed and sequenced generating 79.5 Mb (260 428 reads).

Article Title: Improved reference genome for the domestic horse increases assembly contiguity and composition
Article Snippet: .. The size-selected library was quantified with Qubit (ThermoFisher) and run on an Agilent bioanalyzer DNA high-sensitivity chip (Agilent, Santa Clara, CA, USA) to confirm the presence of DNA fragments of the expected size range. .. It was further quantitated by qPCR on a Bio-Rad CFX Connect Real-Time System (Bio-Rad Laboratories, Inc., Hercules, CA, USA) prior to sequencing for maximization of the number of clusters in the sequencing flowcell.

Article Title: A limited capacity for microglial repopulation in the adult brain
Article Snippet: .. The resulting libraries were validated by qPCR and sized by Agilent Bioanalyzer DNA high sensitivity chip. ..

Article Title: Highly multiplexed AmpliSeq technology identifies novel variation of flowering time-related genes in soybean (Glycine max)
Article Snippet: .. The concentration and size distribution of amplicons in the library were then determined using an Agilent BioAnalyzer DNA High-Sensitivity chip or TapeStation 4200 D1000 chip (Agilent Technologies), according to the instruction of the manufacturer. ..

Article Title: Targeted next generation sequencing identifies novel NOTCH3 gene mutations in CADASIL diagnostics patients
Article Snippet: .. The concentration and size of amplicons was then determined using an Agilent BioAnalyzer DNA High-Sensitivity chip (Agilent Technologies, Santa Clara, CA, USA), according to manufacturers’ instructions. ..

Article Title: Defining the Independence of the Liver Circadian Clock
Article Snippet: .. The resulting libraries were validated by qPCR and sized by Agilent Bioanalyzer DNA high sensitivity chip. .. The concentration for denaturation was 2nM and the final concentration for clustering was 200pM.

Article Title: The RhoJ-BAD signaling network: An Achilles’ heel for BRAF mutant melanomas
Article Snippet: .. The resulting libraries were validated by qPCR and sized by Agilent Bioanalyzer DNA high sensitivity chip. ..

Concentration Assay:

Article Title: Highly multiplexed AmpliSeq technology identifies novel variation of flowering time-related genes in soybean (Glycine max)
Article Snippet: .. The concentration and size distribution of amplicons in the library were then determined using an Agilent BioAnalyzer DNA High-Sensitivity chip or TapeStation 4200 D1000 chip (Agilent Technologies), according to the instruction of the manufacturer. ..

Article Title: Targeted next generation sequencing identifies novel NOTCH3 gene mutations in CADASIL diagnostics patients
Article Snippet: .. The concentration and size of amplicons was then determined using an Agilent BioAnalyzer DNA High-Sensitivity chip (Agilent Technologies, Santa Clara, CA, USA), according to manufacturers’ instructions. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Agilent technologies bioanalyzer high sensitivity dna chip
    Small RNA library analysis. ( A – C ) HL small RNA library purification and validation. ( A ) Representative samples of amplified cDNA prepared from HL-RNA were chromatographed on a 5% PAGE gel. The first lane in each panel is a marker composed of three dsDNA fragments of 145, 160, and 500 bp. The region between 145 and 160 bps, corresponding to adapter-ligated constructs derived from miRNA, was excised from the gel ( B ), and purified. The libraries prepared using these purified constructs were validated by analysis with Agilent <t>Bioanalyzer</t> High Sensitivity <t>DNA</t> Chip. ( C ) A representative electropherogram of a purified library. The peak at ~150 bp indicates the presence of cDNA from HL-miRNA. The peaks other than the ones labeled as miRNAs are the lower and upper markers used by the Bioanalyzer system to determine the size and quantity of the library peak. ( D , E ) Length distribution and annotation of small RNA sequencing reads from HL and BT. ( D ) Sequencing reads from all HL or BT miRNA samples were pooled for the purpose of length distribution and annotation analyses. Length distributions by abundance of sequencing reads from BT (clear bars) or HL (filled bars) are shown. Only reads 18–28 nucleotides in size which map uniquely to the UCSC Genome Browser dm6 (NCBI genome/47) Drosophila melanogaster genome are shown. ( E ) Distribution of RNA biotypes represented as the percentages of reads mapping to the indicated small RNAs. Clear and filled bars represent BT and HL samples, respectively. “Other” represents other Drosophila sncRNAs.
    Bioanalyzer High Sensitivity Dna Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer high sensitivity dna chip/product/Agilent technologies
    Average 95 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer high sensitivity dna chip - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    97
    Agilent technologies dna high sensitivity chip
    <t>DNA</t> methylation levels inversely correlate with expression levels of Esrp2 and Esrp2-as . ( a ) Upper : genomic organization of Esrp2 (dark blue) and Esrp2-as variants 1-4 (v1-4, light blue) and the CGI overlapping the Esrp2 TSS (green). Positions of EPITYPER Amplicons A1-A14 are indicated by horizontal black bars, covering the DMR (pink), the CGI, and CGI shores on both sides. Lower : <t>MCIp-seq</t> detection of methylated DNA fragments in tumors (red) and normal WT mammary glands (blue) of animals at 20 and 24 weeks of age. Each lane represents average reads obtained for three individual samples. ( b , c ) Heatmap of DNA methylation levels in tumor samples ( n =11) and normal mammary gland tissue ( n =9) ( b ), or of various murine cell lines ( c ), with each row representing one individual sample and each column one CpG unit comprising of 1 to 4 individual CpG sites. Methylation levels are depicted by a color-coded gradient from 0% (light yellow) to 100% methylation (blue). Gray squares indicate failed measurements. ( d , e ) Correlation between average methylation of amplicons A1-A14 and Esrp2/Esrp2-as expression levels normalized to three reference genes, calculated by Spearman’s rank correlation for tumor/normal tissues ( d ) and cell lines ( e ). * P
    Dna High Sensitivity Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna high sensitivity chip/product/Agilent technologies
    Average 97 stars, based on 122 article reviews
    Price from $9.99 to $1999.99
    dna high sensitivity chip - by Bioz Stars, 2020-05
    97/100 stars
      Buy from Supplier

    86
    Agilent technologies bioanalyzer 2100 high sensitivity dna chips
    Standards for MspI digestion and progressive PCR. A) MspI digestion of human genomic <t>DNA</t> isolated from human post-mortem brain tissues. DNA (200 ng) was digested by MspI and run on a 4–20% precast polyacrylamide gel and stained with EtBr. Arrows show three satellite DNA bands characteristic of this enzymatic digestion. B) Agilent 2100 Bioanalyzer chromatogram of MspI digested genomic DNA. C) <t>Bioanalyzer</t> 2100 image of a single library from an MspI digested DNA sample. Notice that the satellite bands (indicated by arrows) are still visible on the Bioanalyzer image. D) Progressive PCR amplification combined with limited PCR extension time allows for size selection and amplification of six bisulfite converted libraries (Lanes 1–5 are distinct RRBS libraries; lane 6 (‘C’) is a negative control). After different progressive PCR cycles (18X, 22X, 24X, or 26X – the same libraries are shown for each cycle number) band intensity increases as cycle number increases. Arrows indicate the three satellite DNA bands that are still visible in these libraries.
    Bioanalyzer 2100 High Sensitivity Dna Chips, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer 2100 high sensitivity dna chips/product/Agilent technologies
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100 high sensitivity dna chips - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Small RNA library analysis. ( A – C ) HL small RNA library purification and validation. ( A ) Representative samples of amplified cDNA prepared from HL-RNA were chromatographed on a 5% PAGE gel. The first lane in each panel is a marker composed of three dsDNA fragments of 145, 160, and 500 bp. The region between 145 and 160 bps, corresponding to adapter-ligated constructs derived from miRNA, was excised from the gel ( B ), and purified. The libraries prepared using these purified constructs were validated by analysis with Agilent Bioanalyzer High Sensitivity DNA Chip. ( C ) A representative electropherogram of a purified library. The peak at ~150 bp indicates the presence of cDNA from HL-miRNA. The peaks other than the ones labeled as miRNAs are the lower and upper markers used by the Bioanalyzer system to determine the size and quantity of the library peak. ( D , E ) Length distribution and annotation of small RNA sequencing reads from HL and BT. ( D ) Sequencing reads from all HL or BT miRNA samples were pooled for the purpose of length distribution and annotation analyses. Length distributions by abundance of sequencing reads from BT (clear bars) or HL (filled bars) are shown. Only reads 18–28 nucleotides in size which map uniquely to the UCSC Genome Browser dm6 (NCBI genome/47) Drosophila melanogaster genome are shown. ( E ) Distribution of RNA biotypes represented as the percentages of reads mapping to the indicated small RNAs. Clear and filled bars represent BT and HL samples, respectively. “Other” represents other Drosophila sncRNAs.

    Journal: Genomics Insights

    Article Title: MicroRNAs Circulate in the Hemolymph of Drosophila and Accumulate Relative to Tissue microRNAs in an Age-Dependent Manner

    doi: 10.4137/GEI.S38147

    Figure Lengend Snippet: Small RNA library analysis. ( A – C ) HL small RNA library purification and validation. ( A ) Representative samples of amplified cDNA prepared from HL-RNA were chromatographed on a 5% PAGE gel. The first lane in each panel is a marker composed of three dsDNA fragments of 145, 160, and 500 bp. The region between 145 and 160 bps, corresponding to adapter-ligated constructs derived from miRNA, was excised from the gel ( B ), and purified. The libraries prepared using these purified constructs were validated by analysis with Agilent Bioanalyzer High Sensitivity DNA Chip. ( C ) A representative electropherogram of a purified library. The peak at ~150 bp indicates the presence of cDNA from HL-miRNA. The peaks other than the ones labeled as miRNAs are the lower and upper markers used by the Bioanalyzer system to determine the size and quantity of the library peak. ( D , E ) Length distribution and annotation of small RNA sequencing reads from HL and BT. ( D ) Sequencing reads from all HL or BT miRNA samples were pooled for the purpose of length distribution and annotation analyses. Length distributions by abundance of sequencing reads from BT (clear bars) or HL (filled bars) are shown. Only reads 18–28 nucleotides in size which map uniquely to the UCSC Genome Browser dm6 (NCBI genome/47) Drosophila melanogaster genome are shown. ( E ) Distribution of RNA biotypes represented as the percentages of reads mapping to the indicated small RNAs. Clear and filled bars represent BT and HL samples, respectively. “Other” represents other Drosophila sncRNAs.

    Article Snippet: These cDNA were excised from the PAGE gel , purified, and analyzed with the Agilent Bioanalyzer High Sensitivity DNA Chip ( ).

    Techniques: Purification, Amplification, Polyacrylamide Gel Electrophoresis, Marker, Construct, Derivative Assay, Chromatin Immunoprecipitation, Labeling, RNA Sequencing Assay, Sequencing

    The workflow of L1Hs-targeted enrichment library preparation (A) Double-strand genomic DNA (blue) is extracted from a Korean individual genome (KPGP9). Red boxes indicate the regions where the probe binds to the 3′ UTR of L1Hs elements. (B) Genomic DNA is fragmented by aquatic ultrasonic wave of the Covaris S2 system. Sheared DNAs have an average size of 550 bp, which is suitable for HiSeq sequencing. (C) The Illumina’s adaptor (green) is ligated at both ends of the fragmented DNAs. (D) The adaptor-ligated DNAs is hybridized with the L1Hs-targeted probe (red and orange). Only the presence of the L1Hs 3′ UTR allows the sequence-specific binding of the probe. (E) Targeted DNA fragments are selectively elongated from the probe-binding strands. (F) Because the probe sequence attached to the L1Hs 3′ UTR and the Illumina’s adaptor sequences at both ends are known, targeted DNAs are enriched by PCR with the primer set. After library construction, the final product is confirmed using the Agilent Bioanalyzer High Sensitivity chip assay.

    Journal: Molecules and Cells

    Article Title: Novel Discovery of LINE-1 in a Korean Individual by a Target Enrichment Method

    doi: 10.14348/molcells.2018.0351

    Figure Lengend Snippet: The workflow of L1Hs-targeted enrichment library preparation (A) Double-strand genomic DNA (blue) is extracted from a Korean individual genome (KPGP9). Red boxes indicate the regions where the probe binds to the 3′ UTR of L1Hs elements. (B) Genomic DNA is fragmented by aquatic ultrasonic wave of the Covaris S2 system. Sheared DNAs have an average size of 550 bp, which is suitable for HiSeq sequencing. (C) The Illumina’s adaptor (green) is ligated at both ends of the fragmented DNAs. (D) The adaptor-ligated DNAs is hybridized with the L1Hs-targeted probe (red and orange). Only the presence of the L1Hs 3′ UTR allows the sequence-specific binding of the probe. (E) Targeted DNA fragments are selectively elongated from the probe-binding strands. (F) Because the probe sequence attached to the L1Hs 3′ UTR and the Illumina’s adaptor sequences at both ends are known, targeted DNAs are enriched by PCR with the primer set. After library construction, the final product is confirmed using the Agilent Bioanalyzer High Sensitivity chip assay.

    Article Snippet: After ligation purification, the quality of each library was assessed by using a Bioanalyzer high Sensitivity DNA chip (Agilent Technologies, USA) ( ).

    Techniques: Sequencing, Binding Assay, Polymerase Chain Reaction, Chromatin Immunoprecipitation

    DNA methylation levels inversely correlate with expression levels of Esrp2 and Esrp2-as . ( a ) Upper : genomic organization of Esrp2 (dark blue) and Esrp2-as variants 1-4 (v1-4, light blue) and the CGI overlapping the Esrp2 TSS (green). Positions of EPITYPER Amplicons A1-A14 are indicated by horizontal black bars, covering the DMR (pink), the CGI, and CGI shores on both sides. Lower : MCIp-seq detection of methylated DNA fragments in tumors (red) and normal WT mammary glands (blue) of animals at 20 and 24 weeks of age. Each lane represents average reads obtained for three individual samples. ( b , c ) Heatmap of DNA methylation levels in tumor samples ( n =11) and normal mammary gland tissue ( n =9) ( b ), or of various murine cell lines ( c ), with each row representing one individual sample and each column one CpG unit comprising of 1 to 4 individual CpG sites. Methylation levels are depicted by a color-coded gradient from 0% (light yellow) to 100% methylation (blue). Gray squares indicate failed measurements. ( d , e ) Correlation between average methylation of amplicons A1-A14 and Esrp2/Esrp2-as expression levels normalized to three reference genes, calculated by Spearman’s rank correlation for tumor/normal tissues ( d ) and cell lines ( e ). * P

    Journal: Oncogene

    Article Title: Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer

    doi: 10.1038/onc.2017.246

    Figure Lengend Snippet: DNA methylation levels inversely correlate with expression levels of Esrp2 and Esrp2-as . ( a ) Upper : genomic organization of Esrp2 (dark blue) and Esrp2-as variants 1-4 (v1-4, light blue) and the CGI overlapping the Esrp2 TSS (green). Positions of EPITYPER Amplicons A1-A14 are indicated by horizontal black bars, covering the DMR (pink), the CGI, and CGI shores on both sides. Lower : MCIp-seq detection of methylated DNA fragments in tumors (red) and normal WT mammary glands (blue) of animals at 20 and 24 weeks of age. Each lane represents average reads obtained for three individual samples. ( b , c ) Heatmap of DNA methylation levels in tumor samples ( n =11) and normal mammary gland tissue ( n =9) ( b ), or of various murine cell lines ( c ), with each row representing one individual sample and each column one CpG unit comprising of 1 to 4 individual CpG sites. Methylation levels are depicted by a color-coded gradient from 0% (light yellow) to 100% methylation (blue). Gray squares indicate failed measurements. ( d , e ) Correlation between average methylation of amplicons A1-A14 and Esrp2/Esrp2-as expression levels normalized to three reference genes, calculated by Spearman’s rank correlation for tumor/normal tissues ( d ) and cell lines ( e ). * P

    Article Snippet: Size distribution was confirmed on a DNA High sensitivity Chip (Agilent Bioanalyzer), before proceeding with MCIp reaction using a SX8G-V52 robot (Diagenode, Liège, Belgium) for automated processing.

    Techniques: DNA Methylation Assay, Expressing, Methylation

    Standards for MspI digestion and progressive PCR. A) MspI digestion of human genomic DNA isolated from human post-mortem brain tissues. DNA (200 ng) was digested by MspI and run on a 4–20% precast polyacrylamide gel and stained with EtBr. Arrows show three satellite DNA bands characteristic of this enzymatic digestion. B) Agilent 2100 Bioanalyzer chromatogram of MspI digested genomic DNA. C) Bioanalyzer 2100 image of a single library from an MspI digested DNA sample. Notice that the satellite bands (indicated by arrows) are still visible on the Bioanalyzer image. D) Progressive PCR amplification combined with limited PCR extension time allows for size selection and amplification of six bisulfite converted libraries (Lanes 1–5 are distinct RRBS libraries; lane 6 (‘C’) is a negative control). After different progressive PCR cycles (18X, 22X, 24X, or 26X – the same libraries are shown for each cycle number) band intensity increases as cycle number increases. Arrows indicate the three satellite DNA bands that are still visible in these libraries.

    Journal: BMC Genomics

    Article Title: BisQC: an operational pipeline for multiplexed bisulfite sequencing

    doi: 10.1186/1471-2164-15-290

    Figure Lengend Snippet: Standards for MspI digestion and progressive PCR. A) MspI digestion of human genomic DNA isolated from human post-mortem brain tissues. DNA (200 ng) was digested by MspI and run on a 4–20% precast polyacrylamide gel and stained with EtBr. Arrows show three satellite DNA bands characteristic of this enzymatic digestion. B) Agilent 2100 Bioanalyzer chromatogram of MspI digested genomic DNA. C) Bioanalyzer 2100 image of a single library from an MspI digested DNA sample. Notice that the satellite bands (indicated by arrows) are still visible on the Bioanalyzer image. D) Progressive PCR amplification combined with limited PCR extension time allows for size selection and amplification of six bisulfite converted libraries (Lanes 1–5 are distinct RRBS libraries; lane 6 (‘C’) is a negative control). After different progressive PCR cycles (18X, 22X, 24X, or 26X – the same libraries are shown for each cycle number) band intensity increases as cycle number increases. Arrows indicate the three satellite DNA bands that are still visible in these libraries.

    Article Snippet: After purification, 1 μL of the purified library was used for quality control using an Agilent Bioanalyzer 2100 High Sensitivity DNA Chips.

    Techniques: Polymerase Chain Reaction, Isolation, Staining, Amplification, Selection, Negative Control