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Agilent technologies evs
Bioanalyzer profile and small RNA sequencing of BM-MSC derived <t>EVs</t> Representative bioanalyzer profile of the <t>RNAs</t> contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.
Evs, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 16637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/evs/product/Agilent technologies
Average 92 stars, based on 16637 article reviews
Price from $9.99 to $1999.99
evs - by Bioz Stars, 2020-07
92/100 stars

Images

1) Product Images from "MiRNAs and piRNAs from bone marrow mesenchymal stem cell extracellular vesicles induce cell survival and inhibit cell differentiation of cord blood hematopoietic stem cells: a new insight in transplantation"

Article Title: MiRNAs and piRNAs from bone marrow mesenchymal stem cell extracellular vesicles induce cell survival and inhibit cell differentiation of cord blood hematopoietic stem cells: a new insight in transplantation

Journal: Oncotarget

doi: 10.18632/oncotarget.6791

Bioanalyzer profile and small RNA sequencing of BM-MSC derived EVs Representative bioanalyzer profile of the RNAs contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.
Figure Legend Snippet: Bioanalyzer profile and small RNA sequencing of BM-MSC derived EVs Representative bioanalyzer profile of the RNAs contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.

Techniques Used: RNA Sequencing Assay, Derivative Assay, Fluorescence, Sequencing, Expressing

2) Product Images from "MiRNAs and piRNAs from bone marrow mesenchymal stem cell extracellular vesicles induce cell survival and inhibit cell differentiation of cord blood hematopoietic stem cells: a new insight in transplantation"

Article Title: MiRNAs and piRNAs from bone marrow mesenchymal stem cell extracellular vesicles induce cell survival and inhibit cell differentiation of cord blood hematopoietic stem cells: a new insight in transplantation

Journal: Oncotarget

doi: 10.18632/oncotarget.6791

Bioanalyzer profile and small RNA sequencing of BM-MSC derived EVs Representative bioanalyzer profile of the RNAs contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.
Figure Legend Snippet: Bioanalyzer profile and small RNA sequencing of BM-MSC derived EVs Representative bioanalyzer profile of the RNAs contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.

Techniques Used: RNA Sequencing Assay, Derivative Assay, Fluorescence, Sequencing, Expressing

3) Product Images from "MiRNAs and piRNAs from bone marrow mesenchymal stem cell extracellular vesicles induce cell survival and inhibit cell differentiation of cord blood hematopoietic stem cells: a new insight in transplantation"

Article Title: MiRNAs and piRNAs from bone marrow mesenchymal stem cell extracellular vesicles induce cell survival and inhibit cell differentiation of cord blood hematopoietic stem cells: a new insight in transplantation

Journal: Oncotarget

doi: 10.18632/oncotarget.6791

Bioanalyzer profile and small RNA sequencing of BM-MSC derived EVs Representative bioanalyzer profile of the RNAs contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.
Figure Legend Snippet: Bioanalyzer profile and small RNA sequencing of BM-MSC derived EVs Representative bioanalyzer profile of the RNAs contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.

Techniques Used: RNA Sequencing Assay, Derivative Assay, Fluorescence, Sequencing, Expressing

4) Product Images from "Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits"

Article Title: Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits

Journal: Molecular Cancer

doi: 10.1186/1476-4598-11-37

RNA integrity of samples. RNA derived from ( A ) the drug sensitive-recipient cell (CEM), ( B ) drug-resistant VLB 100 cells, ( C ) their isolated MPs (VLBMP) and ( D ) the drug sensitive-recipient cells after MP transfer (CEM + VLBMP) was analysed using Agilent RNA 6000 Nano kit by Agilent 2100 Bioanalyzer. The RIN value of the samples ranged between 6.2-9.2. Data is representative of a typical experiment
Figure Legend Snippet: RNA integrity of samples. RNA derived from ( A ) the drug sensitive-recipient cell (CEM), ( B ) drug-resistant VLB 100 cells, ( C ) their isolated MPs (VLBMP) and ( D ) the drug sensitive-recipient cells after MP transfer (CEM + VLBMP) was analysed using Agilent RNA 6000 Nano kit by Agilent 2100 Bioanalyzer. The RIN value of the samples ranged between 6.2-9.2. Data is representative of a typical experiment

Techniques Used: Derivative Assay, Isolation

5) Product Images from "MiRNAs and piRNAs from bone marrow mesenchymal stem cell extracellular vesicles induce cell survival and inhibit cell differentiation of cord blood hematopoietic stem cells: a new insight in transplantation"

Article Title: MiRNAs and piRNAs from bone marrow mesenchymal stem cell extracellular vesicles induce cell survival and inhibit cell differentiation of cord blood hematopoietic stem cells: a new insight in transplantation

Journal: Oncotarget

doi: 10.18632/oncotarget.6791

Bioanalyzer profile and small RNA sequencing of BM-MSC derived EVs Representative bioanalyzer profile of the RNAs contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.
Figure Legend Snippet: Bioanalyzer profile and small RNA sequencing of BM-MSC derived EVs Representative bioanalyzer profile of the RNAs contained in BM-MSC ( A ) and in BM-MSC-EVs ( B ). The electropherograms show the size distribution in nucleotides (nt) and fluorescence intensity (FU) of total RNA. The short peak at 25 nt is an internal standard. In BM-MSC the most dominant peaks are the 18S and 28S ribosomal RNA, whereas in EVs is the small RNAs peak respect to the absent rRNA peaks. ( C ) Representative bioanalyzer profile of small RNAs performed on EVs derived from BM-MSC showing an enrichment of small RNAs of the size of miRNAs in respect to the cells of origin. Three different samples of cells and EVs were analyzed. ( D ) Pie-charts showing the percentage of small RNA species identified in BM-MSC-EVs by small RNA sequencing. ( E ) Mature miRNA list obtained by sequencing BM-MSC-EVs small RNA content. The 87 microRNAs, obtained by miRanalyzer, were subdivided in quartiles on the base of readCount numbers and the most representative (1st quartile) were used in the integrated analysis with gene expression profile. ( F ) piRNA list obtained by sequencing BM-MSC-EVs small RNA content with readCount more than five.

Techniques Used: RNA Sequencing Assay, Derivative Assay, Fluorescence, Sequencing, Expressing

6) Product Images from "Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits"

Article Title: Microparticle conferred microRNA profiles - implications in the transfer and dominance of cancer traits

Journal: Molecular Cancer

doi: 10.1186/1476-4598-11-37

RNA integrity of samples. RNA derived from ( A ) the drug sensitive-recipient cell (CEM), ( B ) drug-resistant VLB 100 cells, ( C ) their isolated MPs (VLBMP) and ( D ) the drug sensitive-recipient cells after MP transfer (CEM + VLBMP) was analysed using Agilent RNA 6000 Nano kit by Agilent 2100 Bioanalyzer. The RIN value of the samples ranged between 6.2-9.2. Data is representative of a typical experiment
Figure Legend Snippet: RNA integrity of samples. RNA derived from ( A ) the drug sensitive-recipient cell (CEM), ( B ) drug-resistant VLB 100 cells, ( C ) their isolated MPs (VLBMP) and ( D ) the drug sensitive-recipient cells after MP transfer (CEM + VLBMP) was analysed using Agilent RNA 6000 Nano kit by Agilent 2100 Bioanalyzer. The RIN value of the samples ranged between 6.2-9.2. Data is representative of a typical experiment

Techniques Used: Derivative Assay, Isolation

Related Articles

RNA Sequencing Assay:

Article Title: Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
Article Snippet: .. Reverse transcription PCR In addition to the assessment of RNA quality based on the bacterial and eukaryotic ribosomal RNAs using Agilent Bioanalyzer 2100, one-step RT-PCR was applied to the RNA extracted from microdissection tissues in order to evaluate the quality of bacterial mRNA for RNA-seq analysis. .. The one-step RT-PCR (Qiagen®) was used with specific primers (Additional file : Table S1) targeting three housekeeping genes of L. intracellularis .

Article Title: Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
Article Snippet: .. RNA-seq analysis Total RNA was isolated from HUVECs, and RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies). .. RNA-seq libraries were constructed using the SENSE mRNA-Seq Library Prep Kit (Lexogen), according to the manufacturer’s instructions, and were sequenced as 100 bp paired-end runs on the HiSeq 2000 platform (Illumina).

Polymerase Chain Reaction:

Article Title: Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
Article Snippet: .. Reverse transcription PCR In addition to the assessment of RNA quality based on the bacterial and eukaryotic ribosomal RNAs using Agilent Bioanalyzer 2100, one-step RT-PCR was applied to the RNA extracted from microdissection tissues in order to evaluate the quality of bacterial mRNA for RNA-seq analysis. .. The one-step RT-PCR (Qiagen®) was used with specific primers (Additional file : Table S1) targeting three housekeeping genes of L. intracellularis .

Isolation:

Article Title: Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
Article Snippet: .. RNA-seq analysis Total RNA was isolated from HUVECs, and RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies). .. RNA-seq libraries were constructed using the SENSE mRNA-Seq Library Prep Kit (Lexogen), according to the manufacturer’s instructions, and were sequenced as 100 bp paired-end runs on the HiSeq 2000 platform (Illumina).

Laser Capture Microdissection:

Article Title: Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
Article Snippet: .. Reverse transcription PCR In addition to the assessment of RNA quality based on the bacterial and eukaryotic ribosomal RNAs using Agilent Bioanalyzer 2100, one-step RT-PCR was applied to the RNA extracted from microdissection tissues in order to evaluate the quality of bacterial mRNA for RNA-seq analysis. .. The one-step RT-PCR (Qiagen®) was used with specific primers (Additional file : Table S1) targeting three housekeeping genes of L. intracellularis .

Reverse Transcription Polymerase Chain Reaction:

Article Title: Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
Article Snippet: .. Reverse transcription PCR In addition to the assessment of RNA quality based on the bacterial and eukaryotic ribosomal RNAs using Agilent Bioanalyzer 2100, one-step RT-PCR was applied to the RNA extracted from microdissection tissues in order to evaluate the quality of bacterial mRNA for RNA-seq analysis. .. The one-step RT-PCR (Qiagen®) was used with specific primers (Additional file : Table S1) targeting three housekeeping genes of L. intracellularis .

Generated:

Article Title: A qualitative assessment of direct-labeled cDNA products prior to microarray analysis
Article Snippet: .. Fully degraded, partially degraded and intact RNA, generated by titrating varying amounts of RNase, were used to prepare samples for measuring Cy5 incorporation directly into the cDNA by reverse transcription of RNA by the Agilent 2100 Bioanalyzer. .. It should be noted, however, that RNase treatment can be unpredictable, since even slight deviations in temperature and timing of incubation can result in varying degrees of degradation.

Formalin-fixed Paraffin-Embedded:

Article Title: Biobanking: Objectives, Requirements, and Future Challenges—Experiences from the Munich Vascular Biobank
Article Snippet: .. Analysis of RNA Quality from FFPE Biospecimens by RIN and RNA Fragmentation RNA integrity number (RIN) was determined by Agilent 2100 Bioanalyzer and the RNA 6000 Nano Kit (Agilent Technologies, Waldbronn, Germany) in accordance with the manufacturer’s instructions. ..

Sequencing:

Article Title: Decreasing miRNA sequencing bias using a single adapter and circularization approach
Article Snippet: .. Using RealSeq®-AC and TruSeq®, we prepared sequencing libraries from a reference sample of total RNA (Agilent) obtained from nine different human tissues and cell lines. .. We sequenced both libraries to a coverage of ten million reads and counted the number of miRNAs identified (with ten or more reads of each) by each kit at different sequencing coverages by random subsampling (Additional file : Figure S6).

Chromatin Immunoprecipitation:

Article Title: Characterization of human plasma-derived exosomal RNAs by deep sequencing
Article Snippet: .. The quantity and quality of the RNA were determined by Agilent Bioanalyzer 2100 with a Small RNA Chip for exosomal RNA, and a RNA 6000 Pico Kit for cellular RNA (Agilent Technologies, Santa Clara, CA, USA). .. Enzyme protection assay RNA isolated from the plasma exosomes was first incubated at room temperature, either with 30 units/μL of DNase I (QIAGEN) for 10 min or with 10 μg/mL RNase A for 30 min.

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