bigdye xterminator purification kit  (Thermo Fisher)


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    Name:
    BigDye XTerminator Purification Kit
    Description:
    The BigDye XTerminator Purification Kit is a fast simple purification method for DNA sequencing reactions that removes unincorporated BigDye terminators and salts No more dye blobs Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor • Eliminate liquid transfer Use a single plate • Obtain complete dye blob removal • Stabilize the samples before analysis • Improve your sequencing workflow • Perform manual or automated protocols • Get rapid reliable and reproducible results Simple Purification Process Traditional purification methods such as ethanol precipitation require the addition of multiple reagents along with decanting and centrifuging steps The BigDye XTerminator Purification Kit requires the addition of only two reagents which can be added sequentially or premixed • XTerminator Solution Scavenges unincorporated dye terminators and free salts from the post sequencing reaction • SAM Solution Enhances the performance of the XTerminator Solution and stabilizes the post purification reactions The protocol is very simple see figure 1 1 Perform cycle sequencing with BigDye terminators 2 After cycle sequencing centrifuge the reaction plate briefly and then pipette the correct volume of SAM Solution into each plate well 3 Pipette the correct volume of XTerminator Solution into each well 4 Vortex the XTerminator Solution bulk container briefly 5 Using a wide bore pipette tip recommended wide bore tips pipette the correct volume of liquid into each plate well 6 Seal the plate using a heat seal or Clear Adhesive Film P⁄N 4306311 Vortex for 30 minutes recommended 96 well microplate vortexers and 384 well microplage vortexers then centrifuge the reaction plate briefly 7 Place the reaction plate in the Applied Biosystems DNA analyzer Select run module and run plate Excellent Long Fragment RecoveryYou won t sacrifice sequence length when you use the BigDye XTerminator Purification Kit Figure 2 shows data from the DNA Technology Unit Plant Biotechnology Institute NRC PBI Saskatoon Canada The Q20 base read 1044 bp Improved Short Fragment RecoveryUse the BigDye XTerminator Purification Kit to obtain higher quality data Figure 3 shows a comparison between the BigDye XTerminator Purification Kit and ethanol precipitation based purification performed at the DNA Technology Unit Plant Biotechnology Institute NRC PBI Saskatoon Canada The BigDye XTerminator Purification Kit results in superior short fragment recovery Ethanol purification produces sequence data marred by dye blobs Increased StabilitySealed reaction plates are stable for 48 hours at room temperature or 10 days at 4°C You can re run the same sample in case of a power interruption computer malfunction etc without having to repeat the experiment This increases your flexibility when loading a reaction plate on a CE instrument Run Modules AvailableApplied Biosystems provides download able run modules for use with the BigDye XTerminator Purification Kit and Data Collection Software These modules are designed for the 3100 3100 Avant with Data Collection v2 0 or above 3130⁄3130xl and 3730⁄3730xl Analyzers The software modules for the BigDye XTerminator Purification Kit are compatible with the Windows 2000 and Windows XP operating systems and are available on our software download page http ⁄⁄www appliedbiosystems com⁄absite⁄us⁄en⁄home⁄support⁄software⁄dna sequencing⁄bigdye xterm purif kit html For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4376484
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Extraction|Kits & Reagents for Sanger Sequencing|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Sequencing Reaction Purification|Sequencing
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher bigdye xterminator purification kit
    The BigDye XTerminator Purification Kit is a fast simple purification method for DNA sequencing reactions that removes unincorporated BigDye terminators and salts No more dye blobs Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor • Eliminate liquid transfer Use a single plate • Obtain complete dye blob removal • Stabilize the samples before analysis • Improve your sequencing workflow • Perform manual or automated protocols • Get rapid reliable and reproducible results Simple Purification Process Traditional purification methods such as ethanol precipitation require the addition of multiple reagents along with decanting and centrifuging steps The BigDye XTerminator Purification Kit requires the addition of only two reagents which can be added sequentially or premixed • XTerminator Solution Scavenges unincorporated dye terminators and free salts from the post sequencing reaction • SAM Solution Enhances the performance of the XTerminator Solution and stabilizes the post purification reactions The protocol is very simple see figure 1 1 Perform cycle sequencing with BigDye terminators 2 After cycle sequencing centrifuge the reaction plate briefly and then pipette the correct volume of SAM Solution into each plate well 3 Pipette the correct volume of XTerminator Solution into each well 4 Vortex the XTerminator Solution bulk container briefly 5 Using a wide bore pipette tip recommended wide bore tips pipette the correct volume of liquid into each plate well 6 Seal the plate using a heat seal or Clear Adhesive Film P⁄N 4306311 Vortex for 30 minutes recommended 96 well microplate vortexers and 384 well microplage vortexers then centrifuge the reaction plate briefly 7 Place the reaction plate in the Applied Biosystems DNA analyzer Select run module and run plate Excellent Long Fragment RecoveryYou won t sacrifice sequence length when you use the BigDye XTerminator Purification Kit Figure 2 shows data from the DNA Technology Unit Plant Biotechnology Institute NRC PBI Saskatoon Canada The Q20 base read 1044 bp Improved Short Fragment RecoveryUse the BigDye XTerminator Purification Kit to obtain higher quality data Figure 3 shows a comparison between the BigDye XTerminator Purification Kit and ethanol precipitation based purification performed at the DNA Technology Unit Plant Biotechnology Institute NRC PBI Saskatoon Canada The BigDye XTerminator Purification Kit results in superior short fragment recovery Ethanol purification produces sequence data marred by dye blobs Increased StabilitySealed reaction plates are stable for 48 hours at room temperature or 10 days at 4°C You can re run the same sample in case of a power interruption computer malfunction etc without having to repeat the experiment This increases your flexibility when loading a reaction plate on a CE instrument Run Modules AvailableApplied Biosystems provides download able run modules for use with the BigDye XTerminator Purification Kit and Data Collection Software These modules are designed for the 3100 3100 Avant with Data Collection v2 0 or above 3130⁄3130xl and 3730⁄3730xl Analyzers The software modules for the BigDye XTerminator Purification Kit are compatible with the Windows 2000 and Windows XP operating systems and are available on our software download page http ⁄⁄www appliedbiosystems com⁄absite⁄us⁄en⁄home⁄support⁄software⁄dna sequencing⁄bigdye xterm purif kit html For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/bigdye xterminator purification kit/product/Thermo Fisher
    Average 99 stars, based on 344 article reviews
    Price from $9.99 to $1999.99
    bigdye xterminator purification kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Genetic Profiles of Fluoroquinolone-Resistant Escherichia coli Isolates Obtained from Patients with Cystitis: Phylogeny, Virulence Factors, PAIusp Subtypes, and Mutation Patterns ▿
    Article Snippet: .. The amplified PCR products were processed by using the BigDye Terminator v3.1 cycle sequencing kit, purified by using the BigDye XTerminator kit, and analyzed in an automatic DNA sequencer, GA 3130xl (Applied Biosystems), according to the manufacturer's protocol. ..

    Purification:

    Article Title: Host Range of the Conjugative Transfer System of IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Characterization of Its oriT Region and Relaxase
    Article Snippet: .. After incubation for 30 min at 30°C, the resulting DNA fragments were purified by a BigDye XTerminator purification kit (Thermo Fisher Scientific). .. The purified products were mixed with a GeneScan 500 LIZ size standard (Life Technologies) and separated using an ABI Prism model 3130 xl sequencer.

    Article Title: Drug Resistance-Associated Mutations in Antiretroviral Treatment-Experienced Patients in Kuwait
    Article Snippet: .. Post sequencing PCR purification was performed to remove unbound fluorescent dye deoxy terminators using a BigDye XTerminatorTM Purification Kit (Applied Biosystems). .. The samples were then denatured for 2 min at 95˚C, immediately chilled on ice, and loaded on an ABI 3500 Genetic Analyzer (Applied Biosystems).

    Article Title: Evolutionary regain of lost gene circuit function
    Article Snippet: .. Samples were sequenced at the DNA Sequencing Facility at Stony Brook University using the BigDye Terminator v3.1 sequencing kit, BigDye XTerminator Purification kit and the 3730 DNA Analyzer (all from Applied Biosystems). .. Results were analyzed using the SnapGene software sequence alignment tool.

    Article Title: Sex in the PAC: A hidden affair in dark septate endophytes?
    Article Snippet: .. Dye-labeled fragments were cleaned using BigDye Xterminator Purification kit following manufacturer's instructions (Applied Biosystems). .. Samples were run on an ABI 3130xl DNA Analyser (Applied Biosystems).

    Article Title: Genetic Profiles of Fluoroquinolone-Resistant Escherichia coli Isolates Obtained from Patients with Cystitis: Phylogeny, Virulence Factors, PAIusp Subtypes, and Mutation Patterns ▿
    Article Snippet: .. The amplified PCR products were processed by using the BigDye Terminator v3.1 cycle sequencing kit, purified by using the BigDye XTerminator kit, and analyzed in an automatic DNA sequencer, GA 3130xl (Applied Biosystems), according to the manufacturer's protocol. ..

    Article Title: Exome Sequencing Identifies SMAD3 Mutations as a Cause of Familial Thoracic Aortic Aneurysm and Dissection with Intracranial and Other Arterial Aneurysms
    Article Snippet: .. The sequencing PCR products were purified using the BigDye XTerminator kit (Applied Biosystems, CA) and then loaded on an ABI3730xl sequencing instrument using the Rapid36 run module. .. DNA sequencing results were analyzed using the Mutation Surveyor software (SoftGenetics, PA).

    Article Title: Sequence Analysis of APOA5 Among the Kuwaiti Population Identifies Association of rs2072560, rs2266788, and rs662799 With TG and VLDL Levels
    Article Snippet: .. The extension products were purified using BigDye® XTerminator™ Kit (Life Technologies, Applied Biosystems, USA). .. Capillary electrophoresis was then performed in ABI-3130xl Genetic Analyzer (Life Technologies, Applied Biosystems, USA).

    Sequencing:

    Article Title: Drug Resistance-Associated Mutations in Antiretroviral Treatment-Experienced Patients in Kuwait
    Article Snippet: .. Post sequencing PCR purification was performed to remove unbound fluorescent dye deoxy terminators using a BigDye XTerminatorTM Purification Kit (Applied Biosystems). .. The samples were then denatured for 2 min at 95˚C, immediately chilled on ice, and loaded on an ABI 3500 Genetic Analyzer (Applied Biosystems).

    Article Title: Evolutionary regain of lost gene circuit function
    Article Snippet: .. Samples were sequenced at the DNA Sequencing Facility at Stony Brook University using the BigDye Terminator v3.1 sequencing kit, BigDye XTerminator Purification kit and the 3730 DNA Analyzer (all from Applied Biosystems). .. Results were analyzed using the SnapGene software sequence alignment tool.

    Article Title: Genetic Profiles of Fluoroquinolone-Resistant Escherichia coli Isolates Obtained from Patients with Cystitis: Phylogeny, Virulence Factors, PAIusp Subtypes, and Mutation Patterns ▿
    Article Snippet: .. The amplified PCR products were processed by using the BigDye Terminator v3.1 cycle sequencing kit, purified by using the BigDye XTerminator kit, and analyzed in an automatic DNA sequencer, GA 3130xl (Applied Biosystems), according to the manufacturer's protocol. ..

    Article Title: Exome Sequencing Identifies SMAD3 Mutations as a Cause of Familial Thoracic Aortic Aneurysm and Dissection with Intracranial and Other Arterial Aneurysms
    Article Snippet: .. The sequencing PCR products were purified using the BigDye XTerminator kit (Applied Biosystems, CA) and then loaded on an ABI3730xl sequencing instrument using the Rapid36 run module. .. DNA sequencing results were analyzed using the Mutation Surveyor software (SoftGenetics, PA).

    Article Title: Novel compound heterozygous PKHD1 mutations cause autosomal recessive polycystic kidney disease in a Han Chinese family
    Article Snippet: .. Direct sequencing was performed by Sanger sequencing according to the ABI Big Dye sequencing protocols (cat. no. 4376484; Applied Biosystems; Thermo Fisher Scientific, Inc.) and processed samples were sequenced using an ABI3130XL genetic analyzer (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Incubation:

    Article Title: Host Range of the Conjugative Transfer System of IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Characterization of Its oriT Region and Relaxase
    Article Snippet: .. After incubation for 30 min at 30°C, the resulting DNA fragments were purified by a BigDye XTerminator purification kit (Thermo Fisher Scientific). .. The purified products were mixed with a GeneScan 500 LIZ size standard (Life Technologies) and separated using an ABI Prism model 3130 xl sequencer.

    DNA Sequencing:

    Article Title: Evolutionary regain of lost gene circuit function
    Article Snippet: .. Samples were sequenced at the DNA Sequencing Facility at Stony Brook University using the BigDye Terminator v3.1 sequencing kit, BigDye XTerminator Purification kit and the 3730 DNA Analyzer (all from Applied Biosystems). .. Results were analyzed using the SnapGene software sequence alignment tool.

    Polymerase Chain Reaction:

    Article Title: Drug Resistance-Associated Mutations in Antiretroviral Treatment-Experienced Patients in Kuwait
    Article Snippet: .. Post sequencing PCR purification was performed to remove unbound fluorescent dye deoxy terminators using a BigDye XTerminatorTM Purification Kit (Applied Biosystems). .. The samples were then denatured for 2 min at 95˚C, immediately chilled on ice, and loaded on an ABI 3500 Genetic Analyzer (Applied Biosystems).

    Article Title: Genetic Profiles of Fluoroquinolone-Resistant Escherichia coli Isolates Obtained from Patients with Cystitis: Phylogeny, Virulence Factors, PAIusp Subtypes, and Mutation Patterns ▿
    Article Snippet: .. The amplified PCR products were processed by using the BigDye Terminator v3.1 cycle sequencing kit, purified by using the BigDye XTerminator kit, and analyzed in an automatic DNA sequencer, GA 3130xl (Applied Biosystems), according to the manufacturer's protocol. ..

    Article Title: Exome Sequencing Identifies SMAD3 Mutations as a Cause of Familial Thoracic Aortic Aneurysm and Dissection with Intracranial and Other Arterial Aneurysms
    Article Snippet: .. The sequencing PCR products were purified using the BigDye XTerminator kit (Applied Biosystems, CA) and then loaded on an ABI3730xl sequencing instrument using the Rapid36 run module. .. DNA sequencing results were analyzed using the Mutation Surveyor software (SoftGenetics, PA).

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  • 99
    Thermo Fisher bigdye xterminator purification kit
    Bigdye Xterminator Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye xterminator purification kit/product/Thermo Fisher
    Average 99 stars, based on 344 article reviews
    Price from $9.99 to $1999.99
    bigdye xterminator purification kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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