bigdye terminator v3 1 cycle sequencing kits  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    BigDye Terminator v3 1 Cycle Sequencing Kit
    Description:
    The BigDye Terminator v3 1 Cycle Sequencing Kit s robust highly flexible chemistry is ideal for de novo sequencing resequencing and finishing with PCR product Plasmid Fosmid and BAC templates • Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT rich regions • Get longer higher quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs Get More Uniform Peak Heights Improved AccuracyWith BigDye Terminator v3 1 kit superior chemistry you generate data with uniform peak heights and optimized signal balance This results in longer higher quality reads and more accurate base assignments for heterozygote and mutation detection For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4337454
    Price:
    None
    Applications:
    Kits & Reagents for Sanger Sequencing|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Sequencing
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher bigdye terminator v3 1 cycle sequencing kits
    The BigDye Terminator v3 1 Cycle Sequencing Kit s robust highly flexible chemistry is ideal for de novo sequencing resequencing and finishing with PCR product Plasmid Fosmid and BAC templates • Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT rich regions • Get longer higher quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs Get More Uniform Peak Heights Improved AccuracyWith BigDye Terminator v3 1 kit superior chemistry you generate data with uniform peak heights and optimized signal balance This results in longer higher quality reads and more accurate base assignments for heterozygote and mutation detection For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/bigdye terminator v3 1 cycle sequencing kits/product/Thermo Fisher
    Average 99 stars, based on 136 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v3 1 cycle sequencing kits - by Bioz Stars, 2020-11
    99/100 stars

    Images

    Related Articles

    DNA Extraction:

    Article Title: Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells
    Article Snippet: .. The DNA was electrophoresed in a 3% MetaPhor agarose (FMC Bioproducts, Rockland, ME) gel, excised, purified (Monarch DNA extraction kit, New England Biolabs), and sequenced (BigDye Terminator® version 3.1 cycle sequencing kit, Thermo Fisher Scientific). .. The following reporter gene constructs were generated using the pGL3 luciferase vector (Promega) with the SV40 promoter element removed: (i) empty vector control (CNT), (ii) −1990 to +50 bp of the CAST promoter linked to the luciferase gene, with +1 denoting the first nucleotide of exon 1 (designated CP for canonical promoter region), and (iii) −4026 to +20 bp relative to exon 4, with +1 denoting the first nucleotide exon 4 (designated ALT for NFI-bound alternative promoter).

    Amplification:

    Article Title: Sarcocystis species in wild and domestic sheep (Ovis ammon and Ovis aries) from China
    Article Snippet: .. The amplified PCR products of sarcocysts were sent to Beijing Nuosai Biological Engineering Biotechnology Company for bi-directional sequencing on an ABI PRISM™ 3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. MEGA 6.0 software and was used to construct a phylogenetic tree of the S. tenella isolates using the neighbor-joining method (Kimura two-parameter model).

    Article Title: BAP1 Missense Mutation c.2054 A > T (p.E685V) Completely Disrupts Normal Splicing through Creation of a Novel 5’ Splice Site in a Human Mesothelioma Cell Line
    Article Snippet: .. DNA from colonies was amplified using the BAP1 primers covering cDNA regions of exons 14–17 and subjected to direct DNA sequencing analysis using the forward PCR primer (BigDye Terminator v3.1 Cycle Sequencing kit and 3730 DNA analyzer, Applied Biosystems, Foster City, CA). .. Results Multi-species comparative genomic analysis was used to identify sequence homology at the BAP1 c .2054 A>T (p.E685V) mutation site in ten distantly related species: human, chimpanzee, mouse, rat, dog, cat, rabbit, chicken, xenopus and zebrafish.

    Purification:

    Article Title: Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells
    Article Snippet: .. The DNA was electrophoresed in a 3% MetaPhor agarose (FMC Bioproducts, Rockland, ME) gel, excised, purified (Monarch DNA extraction kit, New England Biolabs), and sequenced (BigDye Terminator® version 3.1 cycle sequencing kit, Thermo Fisher Scientific). .. The following reporter gene constructs were generated using the pGL3 luciferase vector (Promega) with the SV40 promoter element removed: (i) empty vector control (CNT), (ii) −1990 to +50 bp of the CAST promoter linked to the luciferase gene, with +1 denoting the first nucleotide of exon 1 (designated CP for canonical promoter region), and (iii) −4026 to +20 bp relative to exon 4, with +1 denoting the first nucleotide exon 4 (designated ALT for NFI-bound alternative promoter).

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
    Article Snippet: .. DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific). .. Electrophoresis mobility shift assays DNA fragments containing the att sites were amplified with the primer sets P110/P61 (for attP ; 89 bp), P111/P87 (for attB ; 106 bp), P111/P58 (for attL ; 111 bp), and P7/P87 (for attR ; 132 bp).

    Sequencing:

    Article Title: Familial STAG2 germline mutation defines a new human cohesinopathy
    Article Snippet: .. Sanger sequencing was achieved by standard methods using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer. .. Sequencing data was analyzed using the software Sequencher version 4.1.4 (Gene Code).

    Article Title: Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells
    Article Snippet: .. The DNA was electrophoresed in a 3% MetaPhor agarose (FMC Bioproducts, Rockland, ME) gel, excised, purified (Monarch DNA extraction kit, New England Biolabs), and sequenced (BigDye Terminator® version 3.1 cycle sequencing kit, Thermo Fisher Scientific). .. The following reporter gene constructs were generated using the pGL3 luciferase vector (Promega) with the SV40 promoter element removed: (i) empty vector control (CNT), (ii) −1990 to +50 bp of the CAST promoter linked to the luciferase gene, with +1 denoting the first nucleotide of exon 1 (designated CP for canonical promoter region), and (iii) −4026 to +20 bp relative to exon 4, with +1 denoting the first nucleotide exon 4 (designated ALT for NFI-bound alternative promoter).

    Article Title: Sarcocystis species in wild and domestic sheep (Ovis ammon and Ovis aries) from China
    Article Snippet: .. The amplified PCR products of sarcocysts were sent to Beijing Nuosai Biological Engineering Biotechnology Company for bi-directional sequencing on an ABI PRISM™ 3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. MEGA 6.0 software and was used to construct a phylogenetic tree of the S. tenella isolates using the neighbor-joining method (Kimura two-parameter model).

    Article Title: BAP1 Missense Mutation c.2054 A > T (p.E685V) Completely Disrupts Normal Splicing through Creation of a Novel 5’ Splice Site in a Human Mesothelioma Cell Line
    Article Snippet: .. DNA from colonies was amplified using the BAP1 primers covering cDNA regions of exons 14–17 and subjected to direct DNA sequencing analysis using the forward PCR primer (BigDye Terminator v3.1 Cycle Sequencing kit and 3730 DNA analyzer, Applied Biosystems, Foster City, CA). .. Results Multi-species comparative genomic analysis was used to identify sequence homology at the BAP1 c .2054 A>T (p.E685V) mutation site in ten distantly related species: human, chimpanzee, mouse, rat, dog, cat, rabbit, chicken, xenopus and zebrafish.

    Article Title: Molecular characterization of US-like and Asian non-S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013–2016 and PEDVs collected from recurrent outbreaks
    Article Snippet: .. All sequencing reactions were carried out in duplicate, and sequences were determined in both directions using BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems, CA, USA). .. Products were analyzed using ABI PRISM 3130xl Genetic Analyzers (Applied Biosystems).

    Article Title: A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
    Article Snippet: .. Each reaction contained 5 µl sequencing buffer, 5 µl water, 1 µl BigDye Terminator v3.1 (Thermofisher), 1 µM forward or reverse primers and 2.5 µl DNA. .. The sequencing PCR reactions were purified by ethanol precipitation.

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
    Article Snippet: .. DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific). .. Electrophoresis mobility shift assays DNA fragments containing the att sites were amplified with the primer sets P110/P61 (for attP ; 89 bp), P111/P87 (for attB ; 106 bp), P111/P58 (for attL ; 111 bp), and P7/P87 (for attR ; 132 bp).

    Article Title: p-Hydroxybenzoyl-Glucose Is a Zwitter Donor for the Biosynthesis of 7-Polyacylated Anthocyanin in Delphinium [W]
    Article Snippet: .. The sequences of the cDNA fragments were determined using a BigDye-Terminator ver.3.1 cycle sequencing kit and an ABI PRISM 3130xl (Applied Biosystems). .. The 5′ and 3′ cDNA ends of SCPLs and AA7BG-GTs were amplified with a SMARTer RACE cDNA amplification kit (Clontech Laboratories).

    DNA Sequencing:

    Article Title: BAP1 Missense Mutation c.2054 A > T (p.E685V) Completely Disrupts Normal Splicing through Creation of a Novel 5’ Splice Site in a Human Mesothelioma Cell Line
    Article Snippet: .. DNA from colonies was amplified using the BAP1 primers covering cDNA regions of exons 14–17 and subjected to direct DNA sequencing analysis using the forward PCR primer (BigDye Terminator v3.1 Cycle Sequencing kit and 3730 DNA analyzer, Applied Biosystems, Foster City, CA). .. Results Multi-species comparative genomic analysis was used to identify sequence homology at the BAP1 c .2054 A>T (p.E685V) mutation site in ten distantly related species: human, chimpanzee, mouse, rat, dog, cat, rabbit, chicken, xenopus and zebrafish.

    Polymerase Chain Reaction:

    Article Title: Sarcocystis species in wild and domestic sheep (Ovis ammon and Ovis aries) from China
    Article Snippet: .. The amplified PCR products of sarcocysts were sent to Beijing Nuosai Biological Engineering Biotechnology Company for bi-directional sequencing on an ABI PRISM™ 3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. MEGA 6.0 software and was used to construct a phylogenetic tree of the S. tenella isolates using the neighbor-joining method (Kimura two-parameter model).

    Article Title: BAP1 Missense Mutation c.2054 A > T (p.E685V) Completely Disrupts Normal Splicing through Creation of a Novel 5’ Splice Site in a Human Mesothelioma Cell Line
    Article Snippet: .. DNA from colonies was amplified using the BAP1 primers covering cDNA regions of exons 14–17 and subjected to direct DNA sequencing analysis using the forward PCR primer (BigDye Terminator v3.1 Cycle Sequencing kit and 3730 DNA analyzer, Applied Biosystems, Foster City, CA). .. Results Multi-species comparative genomic analysis was used to identify sequence homology at the BAP1 c .2054 A>T (p.E685V) mutation site in ten distantly related species: human, chimpanzee, mouse, rat, dog, cat, rabbit, chicken, xenopus and zebrafish.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher bigdye terminatorv3 1 cycle sequencing
    Bigdye Terminatorv3 1 Cycle Sequencing, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminatorv3 1 cycle sequencing/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bigdye terminatorv3 1 cycle sequencing - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results