bigdye terminator v1 1 cycle sequencing kit  (Thermo Fisher)


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    Thermo Fisher bigdye terminator v1 1 cycle sequencing kit
    Bigdye Terminator V1 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v1 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 775 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v1 1 cycle sequencing kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: The purified PCR products were directly cloned into pGEM-T Easy Vector (Promega, Madison, WI), and transformed into One Shot MAX Efficiency DH5α-T1R competent cells (Thermo Fisher Scientific). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Recombinant HIV-1 clones (HIV-1K65R , HIV-1A114V , HIV-1Y115A , HIV-1F160A , HIV-1M184V HIV-1D185A HIV-1T215F HIV-1D67del/T69G/K70R/L74I/V75T [HIV-167-75 ], and HIV-1D67del/T69G/K70R/L74I/V757T/M184V/T215F [HIV-167-75,184,215 ]) were propagated using HIV-1NL4-3 -based infectious clones generated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Amplification:

    Article Title: Backward compatibility of whole genome sequencing data with MLVA typing using a new MLVAtype shiny application for Vibrio cholerae
    Article Snippet: Sanger-derived versus GeneScan-derived MLVA typing Sanger-derived MLVA typing was performed by sequencing amplicons on both strands on the ABI 3130 GA, using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, USA). .. For Ugandan isolates, the fluorescently labeled amplified products were separated using a 3730xl Automatic Sequencer with the size determined from internal lane standards (LIZ600) by the GeneScan program (Applied Biosystems, Foster City, CA).

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: The extracted proviral DNA was amplified using TaKaRa Ex Taq (TaKaRa Bio Inc., Shiga, Japan) with the following primers: KAPA-1 (5ʹ-GCA GGG CCC CTA GGA AAA AGG GCT GTT GG-3ʹ) and Ksma2.1 (5ʹ-CCA TCC CGG GCT TTA ATT TTA CTG GTA C-3ʹ) for the protease-encoding region, while LTR F2 (5ʹ-GAG ACT CTG GTA ACT AGA GAT C-3ʹ) and PR12 (5ʹ-CTC GTG ACA AAT TTC TAC TAA TGC-3ʹ) for first round PCR and LTR F2 and Ksma2.1 for second round PCR for the entire Gag- and the protease-encoding region ( ). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: .. The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. The data were collected on an ABI 3130 Gene Analyzer (Applied Biosystems).

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Next, the entire protease-coding gene and 951 nucleic acids of the 5′-end of the RT-coding gene were amplified from a drug-selected virus population and were digested with ApaI (gag-coding region) and AgeI (RT-coding region) and were inserted into the infectious clone. .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Article Title: Investigations into the presence of nidoviruses in pythons
    Article Snippet: .. Sequencing reactions of both strands were carried out with the primers used for amplification and the BigDye Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific) on a 3130 Genetic Analyzer (Thermo Fisher Scientific). .. Phylogenetic analysis The newly generated sequences of two full-length snake nidoviruses were aligned to each other as well as to further complete coding genome sequences from different members of the subfamily Tobaniviridae obtained from GenBank using the method MAFFT [ ] as implemented in the Geneious software (version 10.2.1).

    Article Title: A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings
    Article Snippet: Molecular analysis To evaluate ABHD5 exons sequences and their intron/exon boundaries, genomic DNAs of patient and his parents were amplified as previously reported [ ]. .. All PCR products were purified using Nucleo Spin Extract kit (Mcherey-Nagel) and sequenced on 3730 DNA Analyzers by the BigDye® Terminator V1.1 Cycle Sequencing Kit (Applied Biosystems).

    Article Title: TERT promoter mutations are associated with poor prognosis and cell immortalization in meningioma
    Article Snippet: The TERT promoter region of interest containing C228T, C250T, , and C229A mutation sites as well as the single nucleotide polymorphism rs2853669 (c.-245T > C) were amplified using 25 ng genomic DNA, the HotStar Taq Mastermix Kit (Qiagen), the additive Q-solution (Qiagen), and primers S 5ʹ-AGTGGATTCGCGGGCACAGA-3ʹ and AS 5ʹ-CAGCGCTGCCTGAAACTC-3ʹ, resulting in a 235 bp PCR product. .. PCR products were sequenced using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and a 3130 Genetic Analyzer (Applied Biosystems) following standard procedures.

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: We amplified the PrP gene (PRNP ) coding sequence in a 50-μL final volume using 5 μL of extracted DNA, 1× AmpliTaq Gold 360 PCR Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mmol/L MgCl2, 1× 360 GC Enhancer, 200 μmol/L dNTPs, 0.25 μmol/L of forward (5′-GCTGACACCCTCTTTATTTTGCAG-3′) and reverse (5′-GATTAAGAAGATAATGAAAACAGGAAG-3′) primers , and 0.5 μL of AmpliTaq Gold 360 (Applied Biosystems), according to the following amplification protocol: 5 min at 96°C; 30 s at 96°C, 15 s at 57°C, 90 s at 72°C for 40 cycles, and 4 min at 72°C. .. We conducted sequencing reactions by using the BigDye Terminator v1.1 Cycle Sequencing Kit, purified using BigDye XTerminator Purification Kit, and detected with the ABI PRISM 3130 apparatus (all Applied Biosystems).

    Incubation:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: PCR preparations were incubated at 95°C for 1 min, followed by 30 cycles of 95°C for 15 s, 55°C for 20 s, and 72°C for 1 min, with a final extension at 72°C for 7 min. .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Modification:

    Article Title: Backward compatibility of whole genome sequencing data with MLVA typing using a new MLVAtype shiny application for Vibrio cholerae
    Article Snippet: Sanger-derived versus GeneScan-derived MLVA typing Sanger-derived MLVA typing was performed by sequencing amplicons on both strands on the ABI 3130 GA, using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, USA). .. When MLVA profiles were generated according to the method proposed by Kendall et al. [ ], the formula had to be modified to better fit the sequence length of the motif and the position of the primers ( ).

    Transformation Assay:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: The purified PCR products were directly cloned into pGEM-T Easy Vector (Promega, Madison, WI), and transformed into One Shot MAX Efficiency DH5α-T1R competent cells (Thermo Fisher Scientific). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Derivative Assay:

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Thus, the infectious clone contains the entire protease protein and the first 312 amino acids of RT derived from the selected virus population ( ). .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Countercurrent Chromatography:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: The extracted proviral DNA was amplified using TaKaRa Ex Taq (TaKaRa Bio Inc., Shiga, Japan) with the following primers: KAPA-1 (5ʹ-GCA GGG CCC CTA GGA AAA AGG GCT GTT GG-3ʹ) and Ksma2.1 (5ʹ-CCA TCC CGG GCT TTA ATT TTA CTG GTA C-3ʹ) for the protease-encoding region, while LTR F2 (5ʹ-GAG ACT CTG GTA ACT AGA GAT C-3ʹ) and PR12 (5ʹ-CTC GTG ACA AAT TTC TAC TAA TGC-3ʹ) for first round PCR and LTR F2 and Ksma2.1 for second round PCR for the entire Gag- and the protease-encoding region ( ). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Sequencing:

    Article Title: Backward compatibility of whole genome sequencing data with MLVA typing using a new MLVAtype shiny application for Vibrio cholerae
    Article Snippet: .. Sanger-derived versus GeneScan-derived MLVA typing Sanger-derived MLVA typing was performed by sequencing amplicons on both strands on the ABI 3130 GA, using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, USA). ..

    Article Title: Membrane topology of the Drosophila OR83b odorant receptor
    Article Snippet: .. BigDye Terminator v1.1 Cycle Sequencing Kit was from AB Applied Biosystems (Foster City, CA) and oligonucleotides were from CyberGene AB (Stockholm, Sweden). .. Fragments from full-length Or83b cDNA prepared Drosophila heads was modified in two ways during PCR amplification: (i) by the introduction of a 5′ XbaI site, and (ii) by changing the context of the region immediately upstream of the initiator ATG codon to a Kozak consensus ribosome binding sequence, GCCACC ATG G [ ]; both changes were encoded within the 5′PCR primer.

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems). .. The Sanger sequencing was conducted at the CCR Genomics Core at the National Cancer Institute.

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: .. The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. The data were collected on an ABI 3130 Gene Analyzer (Applied Biosystems).

    Article Title: Evaluating sequence data quality from the Swift Accel-Amplicon CFTR Panel
    Article Snippet: .. Sequencing reactions were performed using Applied Biosystems BigDye Terminator v1.1 Cycle Sequencing Kit (#4337449), and were purified using Applied Biosystem Centri-Seq. ..

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute. .. Anti-HIV activity of EFdA and other EFdA-related NRTIs ( ) was examined by performing the methyl thiazol tetrazolium (MTT) and p24 assays, as described previously ( ; ).

    Article Title: Investigations into the presence of nidoviruses in pythons
    Article Snippet: .. Sequencing reactions of both strands were carried out with the primers used for amplification and the BigDye Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific) on a 3130 Genetic Analyzer (Thermo Fisher Scientific). .. Phylogenetic analysis The newly generated sequences of two full-length snake nidoviruses were aligned to each other as well as to further complete coding genome sequences from different members of the subfamily Tobaniviridae obtained from GenBank using the method MAFFT [ ] as implemented in the Geneious software (version 10.2.1).

    Article Title: A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings
    Article Snippet: .. All PCR products were purified using Nucleo Spin Extract kit (Mcherey-Nagel) and sequenced on 3730 DNA Analyzers by the BigDye® Terminator V1.1 Cycle Sequencing Kit (Applied Biosystems). ..

    Article Title: TERT promoter mutations are associated with poor prognosis and cell immortalization in meningioma
    Article Snippet: .. PCR products were sequenced using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and a 3130 Genetic Analyzer (Applied Biosystems) following standard procedures. .. All samples were checked in forward and reverse direction, and SeqScape software v3.0 (Applied Biosystems) was utilized for mutation analysis and fragment assembly.

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: .. We conducted sequencing reactions by using the BigDye Terminator v1.1 Cycle Sequencing Kit, purified using BigDye XTerminator Purification Kit, and detected with the ABI PRISM 3130 apparatus (all Applied Biosystems). .. We analyzed sequences by using Seq Scape version 2.5 (Applied Biosystems).

    Article Title: Clinical significance of BECLIN1 and ATG5 expression in acute myeloid leukemia patients
    Article Snippet: .. All PCR products were sequenced by using a BigDye Terminator V1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. The Statistical Package for Social Sciences software (SPSS Version 16.0) was used to analyze the data in this study.

    Generated:

    Article Title: Backward compatibility of whole genome sequencing data with MLVA typing using a new MLVAtype shiny application for Vibrio cholerae
    Article Snippet: Sanger-derived versus GeneScan-derived MLVA typing Sanger-derived MLVA typing was performed by sequencing amplicons on both strands on the ABI 3130 GA, using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, USA). .. When MLVA profiles were generated according to the method proposed by Kendall et al. [ ], the formula had to be modified to better fit the sequence length of the motif and the position of the primers ( ).

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Recombinant HIV-1 clones (HIV-1K65R , HIV-1A114V , HIV-1Y115A , HIV-1F160A , HIV-1M184V HIV-1D185A HIV-1T215F HIV-1D67del/T69G/K70R/L74I/V75T [HIV-167-75 ], and HIV-1D67del/T69G/K70R/L74I/V757T/M184V/T215F [HIV-167-75,184,215 ]) were propagated using HIV-1NL4-3 -based infectious clones generated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Imaging:

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: Bone radiographic imaging showed hypoplasia of the right clavicle, open anterior fontanelle, and multiple Wormian bones (Fig. a). .. The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: Approximately 20 colonies were selected by pick-up PCR, and plasmids were isolated using a QIAprep spin miniprep kit (Qiagen, Hilden, Germany). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: Genomic DNA was extracted from peripheral blood leukocytes, and polymerase chain reaction (PCR) was performed to amplify each exon using the primer sets shown in Additional file : Table S1. .. The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Evaluating sequence data quality from the Swift Accel-Amplicon CFTR Panel
    Article Snippet: PCR products were purified using Applied Biosystems ExoSAP-IT PCR Product Cleanup Reagent (#78201.1.ML). .. Sequencing reactions were performed using Applied Biosystems BigDye Terminator v1.1 Cycle Sequencing Kit (#4337449), and were purified using Applied Biosystem Centri-Seq.

    Article Title: Investigations into the presence of nidoviruses in pythons
    Article Snippet: The amplified PCR fragments were separated on agarose gels, visualized by staining with ethidium bromide and subsequently excised and purified using the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). .. Sequencing reactions of both strands were carried out with the primers used for amplification and the BigDye Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific) on a 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings
    Article Snippet: .. All PCR products were purified using Nucleo Spin Extract kit (Mcherey-Nagel) and sequenced on 3730 DNA Analyzers by the BigDye® Terminator V1.1 Cycle Sequencing Kit (Applied Biosystems). ..

    Article Title: TERT promoter mutations are associated with poor prognosis and cell immortalization in meningioma
    Article Snippet: .. PCR products were sequenced using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and a 3130 Genetic Analyzer (Applied Biosystems) following standard procedures. .. All samples were checked in forward and reverse direction, and SeqScape software v3.0 (Applied Biosystems) was utilized for mutation analysis and fragment assembly.

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: We amplified the PrP gene (PRNP ) coding sequence in a 50-μL final volume using 5 μL of extracted DNA, 1× AmpliTaq Gold 360 PCR Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mmol/L MgCl2, 1× 360 GC Enhancer, 200 μmol/L dNTPs, 0.25 μmol/L of forward (5′-GCTGACACCCTCTTTATTTTGCAG-3′) and reverse (5′-GATTAAGAAGATAATGAAAACAGGAAG-3′) primers , and 0.5 μL of AmpliTaq Gold 360 (Applied Biosystems), according to the following amplification protocol: 5 min at 96°C; 30 s at 96°C, 15 s at 57°C, 90 s at 72°C for 40 cycles, and 4 min at 72°C. .. We conducted sequencing reactions by using the BigDye Terminator v1.1 Cycle Sequencing Kit, purified using BigDye XTerminator Purification Kit, and detected with the ABI PRISM 3130 apparatus (all Applied Biosystems).

    Article Title: Clinical significance of BECLIN1 and ATG5 expression in acute myeloid leukemia patients
    Article Snippet: .. All PCR products were sequenced by using a BigDye Terminator V1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. The Statistical Package for Social Sciences software (SPSS Version 16.0) was used to analyze the data in this study.

    Recombinant:

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Paragraph title: Recombinant Viruses. ... The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    DNA Extraction:

    Article Title: TERT promoter mutations are associated with poor prognosis and cell immortalization in meningioma
    Article Snippet: Paragraph title: DNA Extraction and TERT Promoter Mutation Analysis ... PCR products were sequenced using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and a 3130 Genetic Analyzer (Applied Biosystems) following standard procedures.

    Marker:

    Article Title: Membrane topology of the Drosophila OR83b odorant receptor
    Article Snippet: [35 S]-Methionine, [14 C]-methylated marker proteins and deoxynucleotides were from GE Healthcare (Uppsala, Sweden). .. BigDye Terminator v1.1 Cycle Sequencing Kit was from AB Applied Biosystems (Foster City, CA) and oligonucleotides were from CyberGene AB (Stockholm, Sweden).

    Mutagenesis:

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. RUNX2 gene analysis revealed a heterozygous mutation ‘p.

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Recombinant HIV-1 clones (HIV-1K65R , HIV-1A114V , HIV-1Y115A , HIV-1F160A , HIV-1M184V HIV-1D185A HIV-1T215F HIV-1D67del/T69G/K70R/L74I/V75T [HIV-167-75 ], and HIV-1D67del/T69G/K70R/L74I/V757T/M184V/T215F [HIV-167-75,184,215 ]) were propagated using HIV-1NL4-3 -based infectious clones generated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Article Title: TERT promoter mutations are associated with poor prognosis and cell immortalization in meningioma
    Article Snippet: Paragraph title: DNA Extraction and TERT Promoter Mutation Analysis ... PCR products were sequenced using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and a 3130 Genetic Analyzer (Applied Biosystems) following standard procedures.

    Article Title: Clinical significance of BECLIN1 and ATG5 expression in acute myeloid leukemia patients
    Article Snippet: Relevant mutation ( NPM1, FLT3-ITD, CEBPα , and c-KIT ) analyses were conducted as described previously [ - ]. .. All PCR products were sequenced by using a BigDye Terminator V1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

    Isolation:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: Approximately 20 colonies were selected by pick-up PCR, and plasmids were isolated using a QIAprep spin miniprep kit (Qiagen, Hilden, Germany). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Article Title: Clinical significance of BECLIN1 and ATG5 expression in acute myeloid leukemia patients
    Article Snippet: Genomic DNA was isolated from BM mononuclear cells. .. All PCR products were sequenced by using a BigDye Terminator V1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

    Labeling:

    Article Title: Backward compatibility of whole genome sequencing data with MLVA typing using a new MLVAtype shiny application for Vibrio cholerae
    Article Snippet: Sanger-derived versus GeneScan-derived MLVA typing Sanger-derived MLVA typing was performed by sequencing amplicons on both strands on the ABI 3130 GA, using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, USA). .. For Ugandan isolates, the fluorescently labeled amplified products were separated using a 3730xl Automatic Sequencer with the size determined from internal lane standards (LIZ600) by the GeneScan program (Applied Biosystems, Foster City, CA).

    Purification:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems). .. The Sanger sequencing was conducted at the CCR Genomics Core at the National Cancer Institute.

    Article Title: Evaluating sequence data quality from the Swift Accel-Amplicon CFTR Panel
    Article Snippet: .. Sequencing reactions were performed using Applied Biosystems BigDye Terminator v1.1 Cycle Sequencing Kit (#4337449), and were purified using Applied Biosystem Centri-Seq. ..

    Article Title: Investigations into the presence of nidoviruses in pythons
    Article Snippet: The amplified PCR fragments were separated on agarose gels, visualized by staining with ethidium bromide and subsequently excised and purified using the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). .. Sequencing reactions of both strands were carried out with the primers used for amplification and the BigDye Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific) on a 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings
    Article Snippet: .. All PCR products were purified using Nucleo Spin Extract kit (Mcherey-Nagel) and sequenced on 3730 DNA Analyzers by the BigDye® Terminator V1.1 Cycle Sequencing Kit (Applied Biosystems). ..

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: .. We conducted sequencing reactions by using the BigDye Terminator v1.1 Cycle Sequencing Kit, purified using BigDye XTerminator Purification Kit, and detected with the ABI PRISM 3130 apparatus (all Applied Biosystems). .. We analyzed sequences by using Seq Scape version 2.5 (Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Investigations into the presence of nidoviruses in pythons
    Article Snippet: The amplification reaction was performed using the SuperScript III One-step RT-PCR Kit (Thermo Fisher Scientific, Waltham, Massachusetts, USA) combined with the primers Nido-Snake-19,971-F (5′ ATC GGA GTC WCA AAA TTC CGA G 3′) and Nido-Snake-21,007-R (5′ CAC GTR TAG CAY TGC TGC TG 3′). .. Sequencing reactions of both strands were carried out with the primers used for amplification and the BigDye Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific) on a 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Gel Extraction:

    Article Title: Investigations into the presence of nidoviruses in pythons
    Article Snippet: The amplified PCR fragments were separated on agarose gels, visualized by staining with ethidium bromide and subsequently excised and purified using the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). .. Sequencing reactions of both strands were carried out with the primers used for amplification and the BigDye Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific) on a 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Activated Clotting Time Assay:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: The extracted proviral DNA was amplified using TaKaRa Ex Taq (TaKaRa Bio Inc., Shiga, Japan) with the following primers: KAPA-1 (5ʹ-GCA GGG CCC CTA GGA AAA AGG GCT GTT GG-3ʹ) and Ksma2.1 (5ʹ-CCA TCC CGG GCT TTA ATT TTA CTG GTA C-3ʹ) for the protease-encoding region, while LTR F2 (5ʹ-GAG ACT CTG GTA ACT AGA GAT C-3ʹ) and PR12 (5ʹ-CTC GTG ACA AAT TTC TAC TAA TGC-3ʹ) for first round PCR and LTR F2 and Ksma2.1 for second round PCR for the entire Gag- and the protease-encoding region ( ). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Plasmid Preparation:

    Article Title: Membrane topology of the Drosophila OR83b odorant receptor
    Article Snippet: Unless otherwise stated, all enzymes, plasmid pGEM1, and the TNT® Quick transcription/translation system were from Promega (Madison, WI). .. BigDye Terminator v1.1 Cycle Sequencing Kit was from AB Applied Biosystems (Foster City, CA) and oligonucleotides were from CyberGene AB (Stockholm, Sweden).

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: The purified PCR products were directly cloned into pGEM-T Easy Vector (Promega, Madison, WI), and transformed into One Shot MAX Efficiency DH5α-T1R competent cells (Thermo Fisher Scientific). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Software:

    Article Title: TERT promoter mutations are associated with poor prognosis and cell immortalization in meningioma
    Article Snippet: PCR products were sequenced using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and a 3130 Genetic Analyzer (Applied Biosystems) following standard procedures. .. All samples were checked in forward and reverse direction, and SeqScape software v3.0 (Applied Biosystems) was utilized for mutation analysis and fragment assembly.

    CTG Assay:

    Article Title: Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2′ Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of Drug Resistance
    Article Snippet: The extracted proviral DNA was amplified using TaKaRa Ex Taq (TaKaRa Bio Inc., Shiga, Japan) with the following primers: KAPA-1 (5ʹ-GCA GGG CCC CTA GGA AAA AGG GCT GTT GG-3ʹ) and Ksma2.1 (5ʹ-CCA TCC CGG GCT TTA ATT TTA CTG GTA C-3ʹ) for the protease-encoding region, while LTR F2 (5ʹ-GAG ACT CTG GTA ACT AGA GAT C-3ʹ) and PR12 (5ʹ-CTC GTG ACA AAT TTC TAC TAA TGC-3ʹ) for first round PCR and LTR F2 and Ksma2.1 for second round PCR for the entire Gag- and the protease-encoding region ( ). .. Purified plasmids were subjected to sequencing using the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems).

    Staining:

    Article Title: Investigations into the presence of nidoviruses in pythons
    Article Snippet: The amplified PCR fragments were separated on agarose gels, visualized by staining with ethidium bromide and subsequently excised and purified using the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). .. Sequencing reactions of both strands were carried out with the primers used for amplification and the BigDye Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific) on a 3130 Genetic Analyzer (Thermo Fisher Scientific).

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    Thermo Fisher bigdye terminator v1 1 cycle sequencing kit
    Bigdye Terminator V1 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v1 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 775 article reviews
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    bigdye terminator v1 1 cycle sequencing kit - by Bioz Stars, 2020-04
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