bigdye terminator v1 1 cycle sequencing kit  (Thermo Fisher)


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    Thermo Fisher bigdye terminator v1 1 cycle sequencing kit
    Bigdye Terminator V1 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v1 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 775 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v1 1 cycle sequencing kit - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Recombinant HIV-1 clones (HIV-1K65R , HIV-1A114V , HIV-1Y115A , HIV-1F160A , HIV-1M184V HIV-1D185A HIV-1T215F HIV-1D67del/T69G/K70R/L74I/V75T [HIV-167-75 ], and HIV-1D67del/T69G/K70R/L74I/V757T/M184V/T215F [HIV-167-75,184,215 ]) were propagated using HIV-1NL4-3 -based infectious clones generated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Amplification:

    Article Title: Genotypes Coding for Low Serum Levels of Mannose-Binding Lectin Are Underrepresented among Individuals Suffering from Noninfectious Systemic Inflammatory Response Syndrome ▿
    Article Snippet: .. The cycling conditions for amplification were 94°C for 8 min, 35 cycles at 94°C for 45 s, 58°C for 30 s, and 72°C for 90 s, and finally 72°C for 10 min. Five microliters of the resulting PCR product was treated with ExoSAP-IT (USB Corporation, Cleveland, OH) and then subjected to direct sequencing with the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, Warrington, United Kingdom) following the manufacturer's instructions with the sense and antisense gene-specific primers mentioned above. ..

    Article Title: Association between Mannose-Binding Lectin Deficiency and Septic Shock following Acute Pyelonephritis Due to Escherichia coli ▿
    Article Snippet: .. The cycling conditions used for amplification of the MBL2 gene were 94°C for 8 min, followed by 35 cycles at 94°C for 45 s, 58°C for 30 s, 72°C for 90 s, and 72°C for 10 min. Portions (5 μl) of the resulting PCR product were treated with ExoSAP-IT (USB Corp., Cleveland, OH) and then subjected to direct sequencing with the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, Warrington, United Kingdom) according to the manufacturer's instructions with the sense and antisense gene-specific primers described above. .. Continuous variables were compared by the Student t test or Mann-Whitney U test when the distribution departed from normality and are given as means (±standard deviations) or medians (range of values), respectively.

    Article Title: Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy ▿
    Article Snippet: The rpoB gene was amplified using the primers +1rB and −4134rB previously described ( ). .. All PCR products were purified by Montage PCR centrifugal filter devices (Millipore, Billerica, MA) and directly sequenced with the ABI PRISM 310 Genetic Analyzer equipment using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA).

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Next, the entire protease-coding gene and 951 nucleic acids of the 5′-end of the RT-coding gene were amplified from a drug-selected virus population and were digested with ApaI (gag-coding region) and AgeI (RT-coding region) and were inserted into the infectious clone. .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: .. The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. The data were collected on an ABI 3130 Gene Analyzer (Applied Biosystems).

    Article Title: A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings
    Article Snippet: Molecular analysis To evaluate ABHD5 exons sequences and their intron/exon boundaries, genomic DNAs of patient and his parents were amplified as previously reported [ ]. .. All PCR products were purified using Nucleo Spin Extract kit (Mcherey-Nagel) and sequenced on 3730 DNA Analyzers by the BigDye® Terminator V1.1 Cycle Sequencing Kit (Applied Biosystems).

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: We amplified the PrP gene (PRNP ) coding sequence in a 50-μL final volume using 5 μL of extracted DNA, 1× AmpliTaq Gold 360 PCR Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mmol/L MgCl2, 1× 360 GC Enhancer, 200 μmol/L dNTPs, 0.25 μmol/L of forward (5′-GCTGACACCCTCTTTATTTTGCAG-3′) and reverse (5′-GATTAAGAAGATAATGAAAACAGGAAG-3′) primers , and 0.5 μL of AmpliTaq Gold 360 (Applied Biosystems), according to the following amplification protocol: 5 min at 96°C; 30 s at 96°C, 15 s at 57°C, 90 s at 72°C for 40 cycles, and 4 min at 72°C. .. We conducted sequencing reactions by using the BigDye Terminator v1.1 Cycle Sequencing Kit, purified using BigDye XTerminator Purification Kit, and detected with the ABI PRISM 3130 apparatus (all Applied Biosystems).

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems). .. Real-time PCRs were performed using the ABI Prism 7500 Sequence Detector in a final volume of 20 μl containing Power SybrGreen (Applied Biosystems) and using specific primers (Supplemental Table 2).

    Article Title: Susceptibility of BAFF-var allele carriers to severe SLE with occurrence of lupus nephritis
    Article Snippet: For polymerase chain reaction (PCR), 50 ng of genomic DNA was amplified in a reaction tube containing 5 pmol of each primer (BAFFVRF, 5′-CTCAGAAGACAGCATCCCGGT-3; BAFFVRR, 5′-GAGAAGAATGCTTGGCCTGCT-3), 1x reaction buffer, 1 U DNA Taq DNA Polymerase (QIAGEN GmbH, Hilden, Germany) and 200 μM of deoxyribonucleotide triphosphate (dNTP Mix; Promega GmbH, Mannheim, Germany). .. Sequencing reactions were performed with the BAFFVRS –5′-TCAGATATGGAACATACTCACAT-3 primer and the BigDye Terminator™ v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Schwerte, Germany), essentially as described by the manufacturer.

    Polymerase Chain Reaction:

    Article Title: Genotypes Coding for Low Serum Levels of Mannose-Binding Lectin Are Underrepresented among Individuals Suffering from Noninfectious Systemic Inflammatory Response Syndrome ▿
    Article Snippet: .. The cycling conditions for amplification were 94°C for 8 min, 35 cycles at 94°C for 45 s, 58°C for 30 s, and 72°C for 90 s, and finally 72°C for 10 min. Five microliters of the resulting PCR product was treated with ExoSAP-IT (USB Corporation, Cleveland, OH) and then subjected to direct sequencing with the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, Warrington, United Kingdom) following the manufacturer's instructions with the sense and antisense gene-specific primers mentioned above. ..

    Article Title: Expression of mRNA for the t;chcomplex polypeptide-1, a subunit of chaperonin CCT, is upregulated in association with increased cold hardiness in Delia antiqua
    Article Snippet: .. One of the differentially expressed gene fragments, the DaTCP -1 fragment, was extracted from the agarose gel, reamplified by PCR (94°C for 3 minutes, 35 cycles of 94°C for 40 seconds, 65°C for 40 seconds, and 72°C for 40 seconds, and 72°C for 5 minutes) with 5′ and 3′ universal primers (Seegene), and sequenced using a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and 5′ universal primer on an ABI PRISM 377HN Sequencer (Applied Biosystems). .. The 5′ and 3′ ends of the DaTCP -1 cDNA were obtained by a modified rapid amplification of cDNA ends (RACE) method using a FirstChoice® RLM RACE Kit (Ambion, Austin, TX, USA).

    Article Title: Association between Mannose-Binding Lectin Deficiency and Septic Shock following Acute Pyelonephritis Due to Escherichia coli ▿
    Article Snippet: .. The cycling conditions used for amplification of the MBL2 gene were 94°C for 8 min, followed by 35 cycles at 94°C for 45 s, 58°C for 30 s, 72°C for 90 s, and 72°C for 10 min. Portions (5 μl) of the resulting PCR product were treated with ExoSAP-IT (USB Corp., Cleveland, OH) and then subjected to direct sequencing with the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, Warrington, United Kingdom) according to the manufacturer's instructions with the sense and antisense gene-specific primers described above. .. Continuous variables were compared by the Student t test or Mann-Whitney U test when the distribution departed from normality and are given as means (±standard deviations) or medians (range of values), respectively.

    Article Title: Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy ▿
    Article Snippet: .. All PCR products were purified by Montage PCR centrifugal filter devices (Millipore, Billerica, MA) and directly sequenced with the ABI PRISM 310 Genetic Analyzer equipment using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. PCR primers and sequencing primers previously described ( ) were used to sequence the whole rpoB gene.

    Article Title: Validation of the Accuracy of Self-Reported ABO Blood Types in the Japan Nurses’ Health Study
    Article Snippet: .. DNA sequences of the PCR products were then analysed on the ABI 3130xl Prism Genetic Analyzer (Applied Biosystems) using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and the BigDye XTerminator Purification Kit (Applied Biosystems). ..

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: Genomic DNA was extracted from peripheral blood leukocytes, and polymerase chain reaction (PCR) was performed to amplify each exon using the primer sets shown in Additional file : Table S1. .. The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

    Article Title: Evaluating sequence data quality from the Swift Accel-Amplicon CFTR Panel
    Article Snippet: PCR products were purified using Applied Biosystems ExoSAP-IT PCR Product Cleanup Reagent (#78201.1.ML). .. Sequencing reactions were performed using Applied Biosystems BigDye Terminator v1.1 Cycle Sequencing Kit (#4337449), and were purified using Applied Biosystem Centri-Seq.

    Article Title: A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings
    Article Snippet: .. All PCR products were purified using Nucleo Spin Extract kit (Mcherey-Nagel) and sequenced on 3730 DNA Analyzers by the BigDye® Terminator V1.1 Cycle Sequencing Kit (Applied Biosystems). ..

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: We amplified the PrP gene (PRNP ) coding sequence in a 50-μL final volume using 5 μL of extracted DNA, 1× AmpliTaq Gold 360 PCR Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mmol/L MgCl2, 1× 360 GC Enhancer, 200 μmol/L dNTPs, 0.25 μmol/L of forward (5′-GCTGACACCCTCTTTATTTTGCAG-3′) and reverse (5′-GATTAAGAAGATAATGAAAACAGGAAG-3′) primers , and 0.5 μL of AmpliTaq Gold 360 (Applied Biosystems), according to the following amplification protocol: 5 min at 96°C; 30 s at 96°C, 15 s at 57°C, 90 s at 72°C for 40 cycles, and 4 min at 72°C. .. We conducted sequencing reactions by using the BigDye Terminator v1.1 Cycle Sequencing Kit, purified using BigDye XTerminator Purification Kit, and detected with the ABI PRISM 3130 apparatus (all Applied Biosystems).

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems). .. Real-time PCRs were performed using the ABI Prism 7500 Sequence Detector in a final volume of 20 μl containing Power SybrGreen (Applied Biosystems) and using specific primers (Supplemental Table 2).

    Article Title: Susceptibility of BAFF-var allele carriers to severe SLE with occurrence of lupus nephritis
    Article Snippet: PCR samples were purified with the QIAquick 96 PCR Purification Kit (QIAGEN GmbH, Hilden, Germany). .. Sequencing reactions were performed with the BAFFVRS –5′-TCAGATATGGAACATACTCACAT-3 primer and the BigDye Terminator™ v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Schwerte, Germany), essentially as described by the manufacturer.

    Article Title: Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
    Article Snippet: .. First round PCR reactions were performed with the OneStep RT-PCR Kit (Qiagen) with the following cycling conditions: reverse transcription at 50 °C for 30 min and 95 °C for 15 min, then 40 cycles of denaturation at 94 °C for 1 min, annealing at 53 °C for 30 s, elongation at 72 °C for 1 min, and final elongation at 72 °C for 10 min. Second round PCR reactions were performed using the GoTaq G2 Flexi DNA Polymerase Kit (Promega, Madison, WI, USA) with an initial denaturation at 95 °C for 5 min, 40 cycles of denaturation at 94 °C for 1 min, annealing 52 °C for 45 s, elongation at 72 °C for 1 min, and final elongation at 72 °C for 10 min. PCR products were sequenced bidirectionally using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) on the ABI Prism 310 genetic analyzer platform (Applied Biosystems). .. Metagenomic cDNA Preparation and Nanopore Sequencing Prior to Nanopore sequencing, lung tissue homogenates were exposed to enrichment protocol as detailed previously [ ], followed by a ribodepletation procedure using The RiboMinus™ Eukaryote System v2 kit (Life Technologies.

    SYBR Green Assay:

    Article Title: Expression of mRNA for the t;chcomplex polypeptide-1, a subunit of chaperonin CCT, is upregulated in association with increased cold hardiness in Delia antiqua
    Article Snippet: The PCR products were separated by 2% agarose gel electrophoresis and stained with SYBR™ Green I (Molecular Probes, Eugene, OR, USA). .. One of the differentially expressed gene fragments, the DaTCP -1 fragment, was extracted from the agarose gel, reamplified by PCR (94°C for 3 minutes, 35 cycles of 94°C for 40 seconds, 65°C for 40 seconds, and 72°C for 40 seconds, and 72°C for 5 minutes) with 5′ and 3′ universal primers (Seegene), and sequenced using a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and 5′ universal primer on an ABI PRISM 377HN Sequencer (Applied Biosystems).

    Modification:

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: For primer pairs that amplified the cDNA carrying the c.319C > G, c.856-2A > G, or c.877_910del mutations, 1× Q-Solution (QIAGEN) was added to the reaction mix, and the PCR conditions were modified as follows: 95°C for 15 minutes followed by 35 cycles at 95°C for 15 seconds and at 62.6°C for 1 minute. .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems).

    Derivative Assay:

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Thus, the infectious clone contains the entire protease protein and the first 312 amino acids of RT derived from the selected virus population ( ). .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Generated:

    Article Title: Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy ▿
    Article Snippet: All PCR products were purified by Montage PCR centrifugal filter devices (Millipore, Billerica, MA) and directly sequenced with the ABI PRISM 310 Genetic Analyzer equipment using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. All consensus sequences generated were then compared to the published B. melitensis 16 M rpoB gene (accession number ) to detect nucleotide diversity.

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Recombinant HIV-1 clones (HIV-1K65R , HIV-1A114V , HIV-1Y115A , HIV-1F160A , HIV-1M184V HIV-1D185A HIV-1T215F HIV-1D67del/T69G/K70R/L74I/V75T [HIV-167-75 ], and HIV-1D67del/T69G/K70R/L74I/V757T/M184V/T215F [HIV-167-75,184,215 ]) were propagated using HIV-1NL4-3 -based infectious clones generated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Imaging:

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: Bone radiographic imaging showed hypoplasia of the right clavicle, open anterior fontanelle, and multiple Wormian bones (Fig. a). .. The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

    Sequencing:

    Article Title: Genotypes Coding for Low Serum Levels of Mannose-Binding Lectin Are Underrepresented among Individuals Suffering from Noninfectious Systemic Inflammatory Response Syndrome ▿
    Article Snippet: .. The cycling conditions for amplification were 94°C for 8 min, 35 cycles at 94°C for 45 s, 58°C for 30 s, and 72°C for 90 s, and finally 72°C for 10 min. Five microliters of the resulting PCR product was treated with ExoSAP-IT (USB Corporation, Cleveland, OH) and then subjected to direct sequencing with the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, Warrington, United Kingdom) following the manufacturer's instructions with the sense and antisense gene-specific primers mentioned above. ..

    Article Title: Expression of mRNA for the t;chcomplex polypeptide-1, a subunit of chaperonin CCT, is upregulated in association with increased cold hardiness in Delia antiqua
    Article Snippet: .. One of the differentially expressed gene fragments, the DaTCP -1 fragment, was extracted from the agarose gel, reamplified by PCR (94°C for 3 minutes, 35 cycles of 94°C for 40 seconds, 65°C for 40 seconds, and 72°C for 40 seconds, and 72°C for 5 minutes) with 5′ and 3′ universal primers (Seegene), and sequenced using a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and 5′ universal primer on an ABI PRISM 377HN Sequencer (Applied Biosystems). .. The 5′ and 3′ ends of the DaTCP -1 cDNA were obtained by a modified rapid amplification of cDNA ends (RACE) method using a FirstChoice® RLM RACE Kit (Ambion, Austin, TX, USA).

    Article Title: Association between Mannose-Binding Lectin Deficiency and Septic Shock following Acute Pyelonephritis Due to Escherichia coli ▿
    Article Snippet: .. The cycling conditions used for amplification of the MBL2 gene were 94°C for 8 min, followed by 35 cycles at 94°C for 45 s, 58°C for 30 s, 72°C for 90 s, and 72°C for 10 min. Portions (5 μl) of the resulting PCR product were treated with ExoSAP-IT (USB Corp., Cleveland, OH) and then subjected to direct sequencing with the BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems, Warrington, United Kingdom) according to the manufacturer's instructions with the sense and antisense gene-specific primers described above. .. Continuous variables were compared by the Student t test or Mann-Whitney U test when the distribution departed from normality and are given as means (±standard deviations) or medians (range of values), respectively.

    Article Title: Membrane topology of the Drosophila OR83b odorant receptor
    Article Snippet: .. BigDye Terminator v1.1 Cycle Sequencing Kit was from AB Applied Biosystems (Foster City, CA) and oligonucleotides were from CyberGene AB (Stockholm, Sweden). .. Fragments from full-length Or83b cDNA prepared Drosophila heads was modified in two ways during PCR amplification: (i) by the introduction of a 5′ XbaI site, and (ii) by changing the context of the region immediately upstream of the initiator ATG codon to a Kozak consensus ribosome binding sequence, GCCACC ATG G [ ]; both changes were encoded within the 5′PCR primer.

    Article Title: Molecular code for protein insertion in the endoplasmic reticulum membrane is similar for Nin-Cout and Nout-Cin transmembrane helices
    Article Snippet: .. The BigDye Terminator v1.1 cycle sequencing kit was from Applied Biosystems, and oligonucleotides were from CyberGene AB. .. The signal peptidase inhibitor N -methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone was from Sigma–Aldrich.

    Article Title: Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy ▿
    Article Snippet: .. All PCR products were purified by Montage PCR centrifugal filter devices (Millipore, Billerica, MA) and directly sequenced with the ABI PRISM 310 Genetic Analyzer equipment using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. PCR primers and sequencing primers previously described ( ) were used to sequence the whole rpoB gene.

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute. .. Anti-HIV activity of EFdA and other EFdA-related NRTIs ( ) was examined by performing the methyl thiazol tetrazolium (MTT) and p24 assays, as described previously ( ; ).

    Article Title: Validation of the Accuracy of Self-Reported ABO Blood Types in the Japan Nurses’ Health Study
    Article Snippet: .. DNA sequences of the PCR products were then analysed on the ABI 3130xl Prism Genetic Analyzer (Applied Biosystems) using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and the BigDye XTerminator Purification Kit (Applied Biosystems). ..

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: .. The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. The data were collected on an ABI 3130 Gene Analyzer (Applied Biosystems).

    Article Title: Evaluating sequence data quality from the Swift Accel-Amplicon CFTR Panel
    Article Snippet: .. Sequencing reactions were performed using Applied Biosystems BigDye Terminator v1.1 Cycle Sequencing Kit (#4337449), and were purified using Applied Biosystem Centri-Seq. ..

    Article Title: A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings
    Article Snippet: .. All PCR products were purified using Nucleo Spin Extract kit (Mcherey-Nagel) and sequenced on 3730 DNA Analyzers by the BigDye® Terminator V1.1 Cycle Sequencing Kit (Applied Biosystems). ..

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: .. We conducted sequencing reactions by using the BigDye Terminator v1.1 Cycle Sequencing Kit, purified using BigDye XTerminator Purification Kit, and detected with the ABI PRISM 3130 apparatus (all Applied Biosystems). .. We analyzed sequences by using Seq Scape version 2.5 (Applied Biosystems).

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems). .. Real-time PCRs were performed using the ABI Prism 7500 Sequence Detector in a final volume of 20 μl containing Power SybrGreen (Applied Biosystems) and using specific primers (Supplemental Table 2).

    Article Title: Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
    Article Snippet: .. First round PCR reactions were performed with the OneStep RT-PCR Kit (Qiagen) with the following cycling conditions: reverse transcription at 50 °C for 30 min and 95 °C for 15 min, then 40 cycles of denaturation at 94 °C for 1 min, annealing at 53 °C for 30 s, elongation at 72 °C for 1 min, and final elongation at 72 °C for 10 min. Second round PCR reactions were performed using the GoTaq G2 Flexi DNA Polymerase Kit (Promega, Madison, WI, USA) with an initial denaturation at 95 °C for 5 min, 40 cycles of denaturation at 94 °C for 1 min, annealing 52 °C for 45 s, elongation at 72 °C for 1 min, and final elongation at 72 °C for 10 min. PCR products were sequenced bidirectionally using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) on the ABI Prism 310 genetic analyzer platform (Applied Biosystems). .. Metagenomic cDNA Preparation and Nanopore Sequencing Prior to Nanopore sequencing, lung tissue homogenates were exposed to enrichment protocol as detailed previously [ ], followed by a ribodepletation procedure using The RiboMinus™ Eukaryote System v2 kit (Life Technologies.

    Recombinant:

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Paragraph title: Recombinant Viruses. ... The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Mutagenesis:

    Article Title: The high genetic barrier of EFdA/MK-8591 stems from strong interactions with the active site of drug-resistant HIV-1 reverse transcriptase
    Article Snippet: Recombinant HIV-1 clones (HIV-1K65R , HIV-1A114V , HIV-1Y115A , HIV-1F160A , HIV-1M184V HIV-1D185A HIV-1T215F HIV-1D67del/T69G/K70R/L74I/V75T [HIV-167-75 ], and HIV-1D67del/T69G/K70R/L74I/V757T/M184V/T215F [HIV-167-75,184,215 ]) were propagated using HIV-1NL4-3 -based infectious clones generated using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). .. The nucleotide sequences of RT-coding gene were verified by the Sanger sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and conducted at the CCR Genomics Core at the National Cancer Institute.

    Article Title: Cleidocranial dysplasia with growth hormone deficiency: a case report
    Article Snippet: The amplified fragments were then gel-purified and directly sequenced with BigDye® Terminator v1.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. RUNX2 gene analysis revealed a heterozygous mutation ‘p.

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems). .. Real-time PCRs were performed using the ABI Prism 7500 Sequence Detector in a final volume of 20 μl containing Power SybrGreen (Applied Biosystems) and using specific primers (Supplemental Table 2).

    Isolation:

    Article Title: Susceptibility of BAFF-var allele carriers to severe SLE with occurrence of lupus nephritis
    Article Snippet: Genotyping Genomic DNA was isolated from 153 whole-blood samples from SSCS using the QIAamp DNA Blood Midi Kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer’s instructions. .. Sequencing reactions were performed with the BAFFVRS –5′-TCAGATATGGAACATACTCACAT-3 primer and the BigDye Terminator™ v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Schwerte, Germany), essentially as described by the manufacturer.

    Purification:

    Article Title: Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy ▿
    Article Snippet: .. All PCR products were purified by Montage PCR centrifugal filter devices (Millipore, Billerica, MA) and directly sequenced with the ABI PRISM 310 Genetic Analyzer equipment using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. PCR primers and sequencing primers previously described ( ) were used to sequence the whole rpoB gene.

    Article Title: Validation of the Accuracy of Self-Reported ABO Blood Types in the Japan Nurses’ Health Study
    Article Snippet: .. DNA sequences of the PCR products were then analysed on the ABI 3130xl Prism Genetic Analyzer (Applied Biosystems) using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and the BigDye XTerminator Purification Kit (Applied Biosystems). ..

    Article Title: Evaluating sequence data quality from the Swift Accel-Amplicon CFTR Panel
    Article Snippet: .. Sequencing reactions were performed using Applied Biosystems BigDye Terminator v1.1 Cycle Sequencing Kit (#4337449), and were purified using Applied Biosystem Centri-Seq. ..

    Article Title: A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings
    Article Snippet: .. All PCR products were purified using Nucleo Spin Extract kit (Mcherey-Nagel) and sequenced on 3730 DNA Analyzers by the BigDye® Terminator V1.1 Cycle Sequencing Kit (Applied Biosystems). ..

    Article Title: Prion Disease in Dromedary Camels, Algeria
    Article Snippet: .. We conducted sequencing reactions by using the BigDye Terminator v1.1 Cycle Sequencing Kit, purified using BigDye XTerminator Purification Kit, and detected with the ABI PRISM 3130 apparatus (all Applied Biosystems). .. We analyzed sequences by using Seq Scape version 2.5 (Applied Biosystems).

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems). .. Real-time PCRs were performed using the ABI Prism 7500 Sequence Detector in a final volume of 20 μl containing Power SybrGreen (Applied Biosystems) and using specific primers (Supplemental Table 2).

    Article Title: Susceptibility of BAFF-var allele carriers to severe SLE with occurrence of lupus nephritis
    Article Snippet: PCR samples were purified with the QIAquick 96 PCR Purification Kit (QIAGEN GmbH, Hilden, Germany). .. Sequencing reactions were performed with the BAFFVRS –5′-TCAGATATGGAACATACTCACAT-3 primer and the BigDye Terminator™ v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Schwerte, Germany), essentially as described by the manufacturer.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: Paragraph title: Semiquantitative RT-PCR. ... To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems).

    Article Title: Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
    Article Snippet: .. First round PCR reactions were performed with the OneStep RT-PCR Kit (Qiagen) with the following cycling conditions: reverse transcription at 50 °C for 30 min and 95 °C for 15 min, then 40 cycles of denaturation at 94 °C for 1 min, annealing at 53 °C for 30 s, elongation at 72 °C for 1 min, and final elongation at 72 °C for 10 min. Second round PCR reactions were performed using the GoTaq G2 Flexi DNA Polymerase Kit (Promega, Madison, WI, USA) with an initial denaturation at 95 °C for 5 min, 40 cycles of denaturation at 94 °C for 1 min, annealing 52 °C for 45 s, elongation at 72 °C for 1 min, and final elongation at 72 °C for 10 min. PCR products were sequenced bidirectionally using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) on the ABI Prism 310 genetic analyzer platform (Applied Biosystems). .. Metagenomic cDNA Preparation and Nanopore Sequencing Prior to Nanopore sequencing, lung tissue homogenates were exposed to enrichment protocol as detailed previously [ ], followed by a ribodepletation procedure using The RiboMinus™ Eukaryote System v2 kit (Life Technologies.

    Helicase-dependent Amplification:

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: PCR products were resolved on 3% Nusieve 3:1 agarose gels (Cambrex) and by capillary electrophoresis with the eGene HDA-GT12 Multi-Channel Genetic Analyzer (eGene Inc.) and quantified by 2 independent methods, i.e., by using ImageJ software ( ) on agarose gels and by using Biocalculator software (eGene Inc.) for runs performed with the HDA-GT12 instrument. .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems).

    Plasmid Preparation:

    Article Title: Membrane topology of the Drosophila OR83b odorant receptor
    Article Snippet: Unless otherwise stated, all enzymes, plasmid pGEM1, and the TNT® Quick transcription/translation system were from Promega (Madison, WI). .. BigDye Terminator v1.1 Cycle Sequencing Kit was from AB Applied Biosystems (Foster City, CA) and oligonucleotides were from CyberGene AB (Stockholm, Sweden).

    Article Title: Molecular code for protein insertion in the endoplasmic reticulum membrane is similar for Nin-Cout and Nout-Cin transmembrane helices
    Article Snippet: Unless otherwise stated, all enzymes, plasmid pGEM1, and the TNT Quick-coupled transcription/translation system were from Promega. .. The BigDye Terminator v1.1 cycle sequencing kit was from Applied Biosystems, and oligonucleotides were from CyberGene AB.

    Software:

    Article Title: Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy ▿
    Article Snippet: All PCR products were purified by Montage PCR centrifugal filter devices (Millipore, Billerica, MA) and directly sequenced with the ABI PRISM 310 Genetic Analyzer equipment using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. The electropherograms were assembled based on the published rpoB sequence of B. melitensis 16 M by the ABI Prism SeqScape Software version 2.0 (Applied Biosystems, Foster City, CA).

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: Measurements for this mutation were obtained by using only the Biocalculator software. .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems).

    Article Title: Susceptibility of BAFF-var allele carriers to severe SLE with occurrence of lupus nephritis
    Article Snippet: Sequencing reactions were performed with the BAFFVRS –5′-TCAGATATGGAACATACTCACAT-3 primer and the BigDye Terminator™ v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Schwerte, Germany), essentially as described by the manufacturer. .. Data analysis was performed with Chromas software version 2.6.5 (Technelysium, South Brisbane, Australia).

    Electrophoresis:

    Article Title: Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy ▿
    Article Snippet: PCR amplifications were analyzed by electrophoresis through a 1% agarose gel with ethidium bromide. .. All PCR products were purified by Montage PCR centrifugal filter devices (Millipore, Billerica, MA) and directly sequenced with the ABI PRISM 310 Genetic Analyzer equipment using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA).

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: PCR products were resolved on 3% Nusieve 3:1 agarose gels (Cambrex) and by capillary electrophoresis with the eGene HDA-GT12 Multi-Channel Genetic Analyzer (eGene Inc.) and quantified by 2 independent methods, i.e., by using ImageJ software ( ) on agarose gels and by using Biocalculator software (eGene Inc.) for runs performed with the HDA-GT12 instrument. .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems).

    Article Title: Susceptibility of BAFF-var allele carriers to severe SLE with occurrence of lupus nephritis
    Article Snippet: Cycling parameters were as follows: initial denaturation at 94 °C for 2 min, 35 cycles at 94 °C for 15 s, annealing at 60 °C for 30 s, at 72 °C for 1 min, and a final extension step at 72 °C for 7 min. PCR products were analyzed by electrophoresis in a 1.5% agarose gel stained with ethidium bromide for identifying the BAFF-specific products (890 bp) for use as a template in sequencing reactions. .. Sequencing reactions were performed with the BAFFVRS –5′-TCAGATATGGAACATACTCACAT-3 primer and the BigDye Terminator™ v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Schwerte, Germany), essentially as described by the manufacturer.

    Agarose Gel Electrophoresis:

    Article Title: Expression of mRNA for the t;chcomplex polypeptide-1, a subunit of chaperonin CCT, is upregulated in association with increased cold hardiness in Delia antiqua
    Article Snippet: .. One of the differentially expressed gene fragments, the DaTCP -1 fragment, was extracted from the agarose gel, reamplified by PCR (94°C for 3 minutes, 35 cycles of 94°C for 40 seconds, 65°C for 40 seconds, and 72°C for 40 seconds, and 72°C for 5 minutes) with 5′ and 3′ universal primers (Seegene), and sequenced using a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and 5′ universal primer on an ABI PRISM 377HN Sequencer (Applied Biosystems). .. The 5′ and 3′ ends of the DaTCP -1 cDNA were obtained by a modified rapid amplification of cDNA ends (RACE) method using a FirstChoice® RLM RACE Kit (Ambion, Austin, TX, USA).

    Article Title: Molecular Epidemiological and Antibiotic Susceptibility Characterization of Brucella Isolates from Humans in Sicily, Italy ▿
    Article Snippet: PCR amplifications were analyzed by electrophoresis through a 1% agarose gel with ethidium bromide. .. All PCR products were purified by Montage PCR centrifugal filter devices (Millipore, Billerica, MA) and directly sequenced with the ABI PRISM 310 Genetic Analyzer equipment using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA).

    Article Title: Validation of the Accuracy of Self-Reported ABO Blood Types in the Japan Nurses’ Health Study
    Article Snippet: A volume of 5 µl of reaction mixture was electrophoresed on a 2% agarose gel and PCR products were visualised by using GelRed (Biotium, Inc., Fremont, CA, USA) to confirm the size of the product and estimate its amount. .. DNA sequences of the PCR products were then analysed on the ABI 3130xl Prism Genetic Analyzer (Applied Biosystems) using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) and the BigDye XTerminator Purification Kit (Applied Biosystems).

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: An exception to this method was for measurements performed in carriers of the c1115_1125del mutation, because agarose gel migration could not effectively separate the wild-type and the mutant mRNA long form and allow the proper use of the ImageJ software. .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems).

    Migration:

    Article Title: Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Article Snippet: An exception to this method was for measurements performed in carriers of the c1115_1125del mutation, because agarose gel migration could not effectively separate the wild-type and the mutant mRNA long form and allow the proper use of the ImageJ software. .. To confirm the identity of the amplified products, and to generate size controls for all mutant and wild-type alleles, PCR products were subcloned into TOPO TA vectors (Invitrogen), purified with the Qiaprep Spin Miniprep Kit (Qiagen), and sequenced using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems).

    Marker:

    Article Title: Membrane topology of the Drosophila OR83b odorant receptor
    Article Snippet: [35 S]-Methionine, [14 C]-methylated marker proteins and deoxynucleotides were from GE Healthcare (Uppsala, Sweden). .. BigDye Terminator v1.1 Cycle Sequencing Kit was from AB Applied Biosystems (Foster City, CA) and oligonucleotides were from CyberGene AB (Stockholm, Sweden).

    Article Title: Molecular code for protein insertion in the endoplasmic reticulum membrane is similar for Nin-Cout and Nout-Cin transmembrane helices
    Article Snippet: [35 S]Met, 14 C-methylated marker proteins, and deoxynucleotides were from GE Healthcare. .. The BigDye Terminator v1.1 cycle sequencing kit was from Applied Biosystems, and oligonucleotides were from CyberGene AB.

    Staining:

    Article Title: Expression of mRNA for the t;chcomplex polypeptide-1, a subunit of chaperonin CCT, is upregulated in association with increased cold hardiness in Delia antiqua
    Article Snippet: The PCR products were separated by 2% agarose gel electrophoresis and stained with SYBR™ Green I (Molecular Probes, Eugene, OR, USA). .. One of the differentially expressed gene fragments, the DaTCP -1 fragment, was extracted from the agarose gel, reamplified by PCR (94°C for 3 minutes, 35 cycles of 94°C for 40 seconds, 65°C for 40 seconds, and 72°C for 40 seconds, and 72°C for 5 minutes) with 5′ and 3′ universal primers (Seegene), and sequenced using a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and 5′ universal primer on an ABI PRISM 377HN Sequencer (Applied Biosystems).

    Article Title: Susceptibility of BAFF-var allele carriers to severe SLE with occurrence of lupus nephritis
    Article Snippet: Cycling parameters were as follows: initial denaturation at 94 °C for 2 min, 35 cycles at 94 °C for 15 s, annealing at 60 °C for 30 s, at 72 °C for 1 min, and a final extension step at 72 °C for 7 min. PCR products were analyzed by electrophoresis in a 1.5% agarose gel stained with ethidium bromide for identifying the BAFF-specific products (890 bp) for use as a template in sequencing reactions. .. Sequencing reactions were performed with the BAFFVRS –5′-TCAGATATGGAACATACTCACAT-3 primer and the BigDye Terminator™ v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Schwerte, Germany), essentially as described by the manufacturer.

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    Thermo Fisher bigdye terminator v1 1 cycle sequencing kit
    Bigdye Terminator V1 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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