bigdye terminator kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bigdye terminator kit
    Bigdye Terminator Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator kit/product/Thermo Fisher
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator kit - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cells
    Article Snippet: .. A full-length cDNA clone was PCR-amplified with Ex Taq Polymerase (Takara Mirus Bio, Madison, WI), purified with the Wizard SV Gel and PCR Clean-Up System (Promega Biosciences, San Luis Obispo, CA), cloned into a pENTR plasmid vector with the Directional TOPO Cloning Kit (Invitrogen), and completely sequenced using BigDye Terminator kit (PE Applied Biosystems, Foster City, CA) and DyeEX 96 Kit (Qiagen Valencia, CA) on ABI 3100 Genetic Analyzer (PE Applied Biosystems). .. The sequence was submitted to Genbank (accession number: ).

    Article Title: Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract
    Article Snippet: The 16S rRNA gene from each of the ASF strains was amplified using bacterium-specific 16S primers 27F and 1492R ( ) and cloned into a pCR2.1 plasmid vector using the Topo-TA cloning kit (Invitrogen). .. The plasmids were sequenced using the BigDye terminator kit (Applied Biosystems) to ensure that the primer regions were not altered due to Taq polymerase error.

    Article Title: Massive Expansion of Gypsy-Like Retrotransposons in Microbotryum Fungi
    Article Snippet: .. Clones were sequenced using the BigDye Terminator kit on an ABI Prism 377XL sequencer (Applied Biosystems) at the University of Massachusetts Amherst DNA sequencing facility. ..

    Article Title: Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey
    Article Snippet: Paragraph title: Cloning of the luciferase gene from C. noctiluca ... The nucleotide sequence was determined using a BigDye Terminator kit (Thermo Fisher Scientific, Waltham, MA, USA) and an ABI PRISM 3130 genetic analyzer (Thermo Fisher Scientific).

    Amplification:

    Article Title: Compound Heterozygosity of the Functionally NullCdh23v-ngt and HypomorphicCdh23ahl Alleles Leads to Early-onset Progressive Hearing Loss in Mice
    Article Snippet: .. The Cdh23ahl mutation was confirmed by DNA sequencing of the products amplified by primer set B (Supplementary Table 1) using a BigDye Terminator kit (Life Technologies, Grand Island, NY, USA) and an Applied Biosystems 3130xl Genetic Analyzer. .. RT-PCR Total RNA was isolated from the inner ear using TRIzol Reagent (Life Technologies) and a TRIzol Plus Purification Kit (Life Technologies) according to the manufacturer’s protocol.

    Article Title: Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract
    Article Snippet: The 16S rRNA gene from each of the ASF strains was amplified using bacterium-specific 16S primers 27F and 1492R ( ) and cloned into a pCR2.1 plasmid vector using the Topo-TA cloning kit (Invitrogen). .. The plasmids were sequenced using the BigDye terminator kit (Applied Biosystems) to ensure that the primer regions were not altered due to Taq polymerase error.

    Article Title: Epidemiology and Clinical Associations of Human Parechovirus Respiratory Infections ▿
    Article Snippet: .. Amplified DNA was directly sequenced using a BigDye Terminator kit (Applied Biosystems, Warrington, United Kingdom) in both directions, from which a complete sequence between positions 2182 and 2437 was assembled. ..

    Article Title: Massive Expansion of Gypsy-Like Retrotransposons in Microbotryum Fungi
    Article Snippet: The thermal profile consisted of initial denaturation at 95°C for 3 min; 30 cycles of amplification with each comprising 95°C for 30 s, touchdown from 64°C to 59°C with a 1°C decrease after every six cycles for 30 s, and 68°C for 1 min; and final extension at 68°C for 7 min. We cloned the resulting products using a TOPO TA cloning kit (Invitrogen), then re-amplified forty clones from each chromosome using the PCR protocol described earlier, except with the initial denaturation step increased to 10 min. We purified the PCR products using ExoSAP-IT (Affymetrix). .. Clones were sequenced using the BigDye Terminator kit on an ABI Prism 377XL sequencer (Applied Biosystems) at the University of Massachusetts Amherst DNA sequencing facility.

    Article Title: Geographical Gradient of the eIF4E Alleles Conferring Resistance to Potyviruses in Pea (Pisum) Germplasm
    Article Snippet: Paragraph title: eIF4E variants amplification ... The PCR products were purified according to Werle et al. protocol and sequenced using a BigDye Terminator kit (Applied Biosystems, UK) by Macrogene (Amsterdam, The Netherlands).

    Article Title: Development of specific primers for Fusarium oxysporum causing damping off of Lilium formolongi
    Article Snippet: .. Amplified products from lily isolates were selected when the size of fragments was distinguished from other isolates, and used for sequencing reactions using a BigDye Terminator kit and a 3700XL Genetic Analyzer (Applied Biosystems). ..

    Article Title: Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey
    Article Snippet: The complete coding sequence (CDS) of the C. noctiluca luciferase (CnocLuc ) was amplified using a high-fidelity PrimeSTAR Max DNA Polymerase (Takara, Shiga, Japan) with gene-specific primer sets CnLuc-f_5UTR (5′-GATCATCCCGGTGATCCAC-3′) and CnLuc-r_3UTR (5′-CTTCTTGGTTCAATGAATGC-3′), designed on the basis of the sequences of the noncoding region of the 5′ and 3′ends. .. The nucleotide sequence was determined using a BigDye Terminator kit (Thermo Fisher Scientific, Waltham, MA, USA) and an ABI PRISM 3130 genetic analyzer (Thermo Fisher Scientific).

    Article Title: In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut
    Article Snippet: .. Following the amplification of DNA inserts with the Illustra TempliPhi DNA Amplification Kit (GE Life Sciences, USA), nucleotide sequences were determined using the BigDye Terminator Kit (Applied Biosystems, USA) and an ABI 3730xl DNA sequencer (Applied Biosystems). ..

    Polymerase Chain Reaction:

    Article Title: Allele-Specific Polymerase Chain Reaction for the Imatinib-Resistant KIT D816V and D816F Mutations in Mastocytosis and Acute Myelogenous Leukemia
    Article Snippet: PCR products were routinely analyzed on a denaturing HPLC system (WAVE; Transgenomic, Inc., Omaha, NE), as previously described., This system allows the detection of DNA heteroduplexes that differ by as little as one nucleotide in 400 bp and is sensitive in the range of 15 to 20% mutant allele. .. DNA sequencing was performed using the BIGDye terminator kit (Applied Biosystems, Foster City, CA) and analyzed on an ABI 310 capillary sequencer.

    Article Title: Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer
    Article Snippet: .. Analysis of Mutations Mutations in p53 were examined from exon 5 to exon 8 by directly sequencing PCR products using the Bigdye Terminator Kit (Applied Biosystems, Foster, CA, USA). .. Epidermal growth factor receptor (EGFR) mutations were detected using the Therascreen EGFR RGQ PCR kit (Qiagen, Valencia, CA, USA). v-Raf murine sarcoma viral oncogene homolog B (BRAF) mutations were detected using the Cobas 4800 BRAF V600 mutation test (Roche, Branchburg, NJ, USA), whereas KRAS mutation were examined using the Cobas KRAS mutation test (Roche, Branchburg, NJ, USA).

    Article Title: Compound Heterozygosity of the Functionally NullCdh23v-ngt and HypomorphicCdh23ahl Alleles Leads to Early-onset Progressive Hearing Loss in Mice
    Article Snippet: PCR amplification was carried out using a KAPA2G Fast PCR Kit (Kapa Biosystems) and primer set A (Supplementary Table 1: refer to J-STAGE at https://www.jstage.jst.go.jp/browse/expanim) and consisted of 40 cycles at 95°C for 20 s, 60°C for 20 s and 72°C for 5 s; the products were digested with Bst NI (New England BioLabs, Ipswich, MA, USA) at 65°C for 1 h and then subjected to 4% agarose gel electrophoresis. .. The Cdh23ahl mutation was confirmed by DNA sequencing of the products amplified by primer set B (Supplementary Table 1) using a BigDye Terminator kit (Life Technologies, Grand Island, NY, USA) and an Applied Biosystems 3130xl Genetic Analyzer.

    Article Title: Molecular Epidemiology and Spatial Distribution of a Waterborne Cryptosporidiosis Outbreak in Australia ▿
    Article Snippet: The PCR mixtures contained 4 mM MgCl2 , 200 nM dNTPs, 200 nM each forward and reverse primer, and 1 U of Red Hot Taq polymerase with 2 μl of DNA template for the primary reaction and 1 μl of the primary PCR product for the secondary reaction. .. Reaction mixtures containing the correct size fragment (∼1,000 bp) were purified as described above and sequenced using an ABI 3130xl genetic analyzer (Applied Biosystems, Foster City, CA) with a BigDye Terminator kit (Applied Biosystems).

    Article Title: Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cells
    Article Snippet: .. A full-length cDNA clone was PCR-amplified with Ex Taq Polymerase (Takara Mirus Bio, Madison, WI), purified with the Wizard SV Gel and PCR Clean-Up System (Promega Biosciences, San Luis Obispo, CA), cloned into a pENTR plasmid vector with the Directional TOPO Cloning Kit (Invitrogen), and completely sequenced using BigDye Terminator kit (PE Applied Biosystems, Foster City, CA) and DyeEX 96 Kit (Qiagen Valencia, CA) on ABI 3100 Genetic Analyzer (PE Applied Biosystems). .. The sequence was submitted to Genbank (accession number: ).

    Article Title: Epidemiology and Clinical Associations of Human Parechovirus Respiratory Infections ▿
    Article Snippet: The RT and PCR cycling conditions for the first round were, sequentially, 43°C for 1 h and 20 cycles of 53°C (1 min) and 55°C (1 min), followed by 70°C for 15 min and 94°C for 2 min. PCR comprised 40 cycles of 94°C (30 s), 50°C (30 s), and 68°C (105 s) and a final extension at 68°C for 5 min. One microliter of the product was transferred for a second PCR, which was performed as described for the 5′ UTR PCR but with the VP3/VP1 inner primers. .. Amplified DNA was directly sequenced using a BigDye Terminator kit (Applied Biosystems, Warrington, United Kingdom) in both directions, from which a complete sequence between positions 2182 and 2437 was assembled.

    Article Title: Massive Expansion of Gypsy-Like Retrotransposons in Microbotryum Fungi
    Article Snippet: The thermal profile consisted of initial denaturation at 95°C for 3 min; 30 cycles of amplification with each comprising 95°C for 30 s, touchdown from 64°C to 59°C with a 1°C decrease after every six cycles for 30 s, and 68°C for 1 min; and final extension at 68°C for 7 min. We cloned the resulting products using a TOPO TA cloning kit (Invitrogen), then re-amplified forty clones from each chromosome using the PCR protocol described earlier, except with the initial denaturation step increased to 10 min. We purified the PCR products using ExoSAP-IT (Affymetrix). .. Clones were sequenced using the BigDye Terminator kit on an ABI Prism 377XL sequencer (Applied Biosystems) at the University of Massachusetts Amherst DNA sequencing facility.

    Article Title: Geographical Gradient of the eIF4E Alleles Conferring Resistance to Potyviruses in Pea (Pisum) Germplasm
    Article Snippet: .. The PCR products were purified according to Werle et al. protocol and sequenced using a BigDye Terminator kit (Applied Biosystems, UK) by Macrogene (Amsterdam, The Netherlands). .. Bioinformatics Primer design and restriction analyses were performed using FastPCR software version 5.1.83 (PrimerDigital Ltd., Finland).

    Article Title: Development of specific primers for Fusarium oxysporum causing damping off of Lilium formolongi
    Article Snippet: The success of PCR was confirmed by the presence or absence of fragments from the DNA of F. oxysporum . .. Amplified products from lily isolates were selected when the size of fragments was distinguished from other isolates, and used for sequencing reactions using a BigDye Terminator kit and a 3700XL Genetic Analyzer (Applied Biosystems).

    Article Title: Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey
    Article Snippet: PCR conditions to amplify CnocLuc CDS were the following: an initial 2.0 min at 96°C, followed by 30 cycles of 10 s at 98°C, 10 s at 52°C, and 40 s at 72°C, with a final extension of 5.0 min at 72°C. .. The nucleotide sequence was determined using a BigDye Terminator kit (Thermo Fisher Scientific, Waltham, MA, USA) and an ABI PRISM 3130 genetic analyzer (Thermo Fisher Scientific).

    Article Title: In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut
    Article Snippet: Ten microliters of PCR mixture, composed of 0.04 ng/μl template DNA, 0.5 pmol/μl primer pairs (Additional file ), 1X PCR buffer (Bioline, UK), 0.2 mM dNTPs, 5 mM MgCl2 , and 0.25 U BioTaq DNA polymerase (Bioline), was used. .. Following the amplification of DNA inserts with the Illustra TempliPhi DNA Amplification Kit (GE Life Sciences, USA), nucleotide sequences were determined using the BigDye Terminator Kit (Applied Biosystems, USA) and an ABI 3730xl DNA sequencer (Applied Biosystems).

    Article Title: Assessment of fish iridoviruses using a novel cell line GS-1, derived from the spleen of orange-spotted grouper Epinephelus coioides (Hamilton) and susceptible to ranavirus and megalocytivirus
    Article Snippet: .. PCR products were sequenced using an ABI PRISM 377 DNA sequencer with a BigDye Terminator Kit (Applied Biosystems, Foster City, CA, U.S.A.). ..

    Article Title: The Murine Norovirus Core Subgenomic RNA Promoter Consists of a Stable Stem-Loop That Can Direct Accurate Initiation of RNA Synthesis
    Article Snippet: .. Sequences of the PCR products were determined using the BigDye Terminator kit and analyzed using the Vector NTI software (Invitrogen). .. Primer extension analysis was performed on RNA isolated from either infected cells or cells transfected with in vitro -transcribed capped RNAs generated from MNV cDNA-containing clones.

    TA Cloning:

    Article Title: Massive Expansion of Gypsy-Like Retrotransposons in Microbotryum Fungi
    Article Snippet: The thermal profile consisted of initial denaturation at 95°C for 3 min; 30 cycles of amplification with each comprising 95°C for 30 s, touchdown from 64°C to 59°C with a 1°C decrease after every six cycles for 30 s, and 68°C for 1 min; and final extension at 68°C for 7 min. We cloned the resulting products using a TOPO TA cloning kit (Invitrogen), then re-amplified forty clones from each chromosome using the PCR protocol described earlier, except with the initial denaturation step increased to 10 min. We purified the PCR products using ExoSAP-IT (Affymetrix). .. Clones were sequenced using the BigDye Terminator kit on an ABI Prism 377XL sequencer (Applied Biosystems) at the University of Massachusetts Amherst DNA sequencing facility.

    Electrophoresis:

    Article Title: Development of specific primers for Fusarium oxysporum causing damping off of Lilium formolongi
    Article Snippet: Amplification was observed by electrophoresis on 2% agarose gels in Tris-acetate EDTA buffer. .. Amplified products from lily isolates were selected when the size of fragments was distinguished from other isolates, and used for sequencing reactions using a BigDye Terminator kit and a 3700XL Genetic Analyzer (Applied Biosystems).

    Microarray:

    Article Title: Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cells
    Article Snippet: Using our DNA microarray data of preimplantation embryos , we identified a Zscan4d gene for its specific expression in 2-cell embryos and found a corresponding cDNA clone (no. C0348C03; ; R1 ES cells, 129 strain) in our mouse cDNA collection ( ). .. A full-length cDNA clone was PCR-amplified with Ex Taq Polymerase (Takara Mirus Bio, Madison, WI), purified with the Wizard SV Gel and PCR Clean-Up System (Promega Biosciences, San Luis Obispo, CA), cloned into a pENTR plasmid vector with the Directional TOPO Cloning Kit (Invitrogen), and completely sequenced using BigDye Terminator kit (PE Applied Biosystems, Foster City, CA) and DyeEX 96 Kit (Qiagen Valencia, CA) on ABI 3100 Genetic Analyzer (PE Applied Biosystems).

    Luciferase:

    Article Title: Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey
    Article Snippet: Paragraph title: Cloning of the luciferase gene from C. noctiluca ... The nucleotide sequence was determined using a BigDye Terminator kit (Thermo Fisher Scientific, Waltham, MA, USA) and an ABI PRISM 3130 genetic analyzer (Thermo Fisher Scientific).

    In Silico:

    Article Title: In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut
    Article Snippet: Paragraph title: Development of transposon markers through in silico polymorphism analysis ... Following the amplification of DNA inserts with the Illustra TempliPhi DNA Amplification Kit (GE Life Sciences, USA), nucleotide sequences were determined using the BigDye Terminator Kit (Applied Biosystems, USA) and an ABI 3730xl DNA sequencer (Applied Biosystems).

    Expressing:

    Article Title: Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cells
    Article Snippet: Using our DNA microarray data of preimplantation embryos , we identified a Zscan4d gene for its specific expression in 2-cell embryos and found a corresponding cDNA clone (no. C0348C03; ; R1 ES cells, 129 strain) in our mouse cDNA collection ( ). .. A full-length cDNA clone was PCR-amplified with Ex Taq Polymerase (Takara Mirus Bio, Madison, WI), purified with the Wizard SV Gel and PCR Clean-Up System (Promega Biosciences, San Luis Obispo, CA), cloned into a pENTR plasmid vector with the Directional TOPO Cloning Kit (Invitrogen), and completely sequenced using BigDye Terminator kit (PE Applied Biosystems, Foster City, CA) and DyeEX 96 Kit (Qiagen Valencia, CA) on ABI 3100 Genetic Analyzer (PE Applied Biosystems).

    High Performance Liquid Chromatography:

    Article Title: Allele-Specific Polymerase Chain Reaction for the Imatinib-Resistant KIT D816V and D816F Mutations in Mastocytosis and Acute Myelogenous Leukemia
    Article Snippet: Paragraph title: HPLC and DNA Sequencing ... DNA sequencing was performed using the BIGDye terminator kit (Applied Biosystems, Foster City, CA) and analyzed on an ABI 310 capillary sequencer.

    Electroporation:

    Article Title: In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut
    Article Snippet: Plasmids were introduced into Escherichia coli ElectroTen-blue (Stratagene, USA) by electroporation. .. Following the amplification of DNA inserts with the Illustra TempliPhi DNA Amplification Kit (GE Life Sciences, USA), nucleotide sequences were determined using the BigDye Terminator Kit (Applied Biosystems, USA) and an ABI 3730xl DNA sequencer (Applied Biosystems).

    Infection:

    Article Title: Assessment of fish iridoviruses using a novel cell line GS-1, derived from the spleen of orange-spotted grouper Epinephelus coioides (Hamilton) and susceptible to ranavirus and megalocytivirus
    Article Snippet: Viral confirmation by conventional PCR The methods were described in previous studies [ ] and also used to confirm the infection of inoculated cells and experimentally challenged fishes. .. PCR products were sequenced using an ABI PRISM 377 DNA sequencer with a BigDye Terminator Kit (Applied Biosystems, Foster City, CA, U.S.A.).

    DNA Sequencing:

    Article Title: Allele-Specific Polymerase Chain Reaction for the Imatinib-Resistant KIT D816V and D816F Mutations in Mastocytosis and Acute Myelogenous Leukemia
    Article Snippet: .. DNA sequencing was performed using the BIGDye terminator kit (Applied Biosystems, Foster City, CA) and analyzed on an ABI 310 capillary sequencer. .. To look for low-abundance KIT exon 17 mutations, amplicons were cloned into Topo plasmids and sequenced individually, as previously described.

    Article Title: Compound Heterozygosity of the Functionally NullCdh23v-ngt and HypomorphicCdh23ahl Alleles Leads to Early-onset Progressive Hearing Loss in Mice
    Article Snippet: .. The Cdh23ahl mutation was confirmed by DNA sequencing of the products amplified by primer set B (Supplementary Table 1) using a BigDye Terminator kit (Life Technologies, Grand Island, NY, USA) and an Applied Biosystems 3130xl Genetic Analyzer. .. RT-PCR Total RNA was isolated from the inner ear using TRIzol Reagent (Life Technologies) and a TRIzol Plus Purification Kit (Life Technologies) according to the manufacturer’s protocol.

    Article Title: Massive Expansion of Gypsy-Like Retrotransposons in Microbotryum Fungi
    Article Snippet: .. Clones were sequenced using the BigDye Terminator kit on an ABI Prism 377XL sequencer (Applied Biosystems) at the University of Massachusetts Amherst DNA sequencing facility. ..

    Sequencing:

    Article Title: Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer
    Article Snippet: .. Analysis of Mutations Mutations in p53 were examined from exon 5 to exon 8 by directly sequencing PCR products using the Bigdye Terminator Kit (Applied Biosystems, Foster, CA, USA). .. Epidermal growth factor receptor (EGFR) mutations were detected using the Therascreen EGFR RGQ PCR kit (Qiagen, Valencia, CA, USA). v-Raf murine sarcoma viral oncogene homolog B (BRAF) mutations were detected using the Cobas 4800 BRAF V600 mutation test (Roche, Branchburg, NJ, USA), whereas KRAS mutation were examined using the Cobas KRAS mutation test (Roche, Branchburg, NJ, USA).

    Article Title: Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cells
    Article Snippet: Based on this full-length cDNA sequence, we designed a primer pair (5′-cctccctgggcttcttggcat-3′; 5′-agctgccaaccagaaagacactgt-3′), which was used to PCR-amplify the full-length cDNA sequence of this gene from 2-cell embryos (B6D2F1 mouse). .. A full-length cDNA clone was PCR-amplified with Ex Taq Polymerase (Takara Mirus Bio, Madison, WI), purified with the Wizard SV Gel and PCR Clean-Up System (Promega Biosciences, San Luis Obispo, CA), cloned into a pENTR plasmid vector with the Directional TOPO Cloning Kit (Invitrogen), and completely sequenced using BigDye Terminator kit (PE Applied Biosystems, Foster City, CA) and DyeEX 96 Kit (Qiagen Valencia, CA) on ABI 3100 Genetic Analyzer (PE Applied Biosystems).

    Article Title: Epidemiology and Clinical Associations of Human Parechovirus Respiratory Infections ▿
    Article Snippet: .. Amplified DNA was directly sequenced using a BigDye Terminator kit (Applied Biosystems, Warrington, United Kingdom) in both directions, from which a complete sequence between positions 2182 and 2437 was assembled. ..

    Article Title: Massive Expansion of Gypsy-Like Retrotransposons in Microbotryum Fungi
    Article Snippet: PCR primers were designed to amplify 938bp of the consensus of an alignment of shotgun sequence reads from Microbotryum strains tiled against a full-length Gypsy -like TE from Laccaria bicolor strain S238N-H82 (gi 170112043): forward primer MvGf 5′ AAAGTCCCCACGGCGTTAT and reverse primer MvGr 5′ GTTGAGAAACTGGGGTTAGCTGT. .. Clones were sequenced using the BigDye Terminator kit on an ABI Prism 377XL sequencer (Applied Biosystems) at the University of Massachusetts Amherst DNA sequencing facility.

    Article Title: Development of specific primers for Fusarium oxysporum causing damping off of Lilium formolongi
    Article Snippet: .. Amplified products from lily isolates were selected when the size of fragments was distinguished from other isolates, and used for sequencing reactions using a BigDye Terminator kit and a 3700XL Genetic Analyzer (Applied Biosystems). ..

    Article Title: Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey
    Article Snippet: .. The nucleotide sequence was determined using a BigDye Terminator kit (Thermo Fisher Scientific, Waltham, MA, USA) and an ABI PRISM 3130 genetic analyzer (Thermo Fisher Scientific). ..

    Fluorescence:

    Article Title: Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract
    Article Snippet: The plasmids were sequenced using the BigDye terminator kit (Applied Biosystems) to ensure that the primer regions were not altered due to Taq polymerase error. .. The concentrations of the linearized plasmid were determined on a Synergy HT microplate fluorescence reader (Bio-Tek, Winooski, Vt.) using the PicoGreen kit (Molecular Probes, Eugene, Oreg.) and dilutions of known concentrations of λ DNA as standards.

    Mutagenesis:

    Article Title: Allele-Specific Polymerase Chain Reaction for the Imatinib-Resistant KIT D816V and D816F Mutations in Mastocytosis and Acute Myelogenous Leukemia
    Article Snippet: PCR products were routinely analyzed on a denaturing HPLC system (WAVE; Transgenomic, Inc., Omaha, NE), as previously described., This system allows the detection of DNA heteroduplexes that differ by as little as one nucleotide in 400 bp and is sensitive in the range of 15 to 20% mutant allele. .. DNA sequencing was performed using the BIGDye terminator kit (Applied Biosystems, Foster City, CA) and analyzed on an ABI 310 capillary sequencer.

    Article Title: Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer
    Article Snippet: Analysis of Mutations Mutations in p53 were examined from exon 5 to exon 8 by directly sequencing PCR products using the Bigdye Terminator Kit (Applied Biosystems, Foster, CA, USA). .. Epidermal growth factor receptor (EGFR) mutations were detected using the Therascreen EGFR RGQ PCR kit (Qiagen, Valencia, CA, USA). v-Raf murine sarcoma viral oncogene homolog B (BRAF) mutations were detected using the Cobas 4800 BRAF V600 mutation test (Roche, Branchburg, NJ, USA), whereas KRAS mutation were examined using the Cobas KRAS mutation test (Roche, Branchburg, NJ, USA).

    Article Title: Compound Heterozygosity of the Functionally NullCdh23v-ngt and HypomorphicCdh23ahl Alleles Leads to Early-onset Progressive Hearing Loss in Mice
    Article Snippet: .. The Cdh23ahl mutation was confirmed by DNA sequencing of the products amplified by primer set B (Supplementary Table 1) using a BigDye Terminator kit (Life Technologies, Grand Island, NY, USA) and an Applied Biosystems 3130xl Genetic Analyzer. .. RT-PCR Total RNA was isolated from the inner ear using TRIzol Reagent (Life Technologies) and a TRIzol Plus Purification Kit (Life Technologies) according to the manufacturer’s protocol.

    Purification:

    Article Title: Molecular Epidemiology and Spatial Distribution of a Waterborne Cryptosporidiosis Outbreak in Australia ▿
    Article Snippet: .. Reaction mixtures containing the correct size fragment (∼1,000 bp) were purified as described above and sequenced using an ABI 3130xl genetic analyzer (Applied Biosystems, Foster City, CA) with a BigDye Terminator kit (Applied Biosystems). .. Nucleotide sequences were analyzed using Geneious version 4.8.2 (Biomatters Ltd., Auckland, New Zealand).

    Article Title: Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cells
    Article Snippet: .. A full-length cDNA clone was PCR-amplified with Ex Taq Polymerase (Takara Mirus Bio, Madison, WI), purified with the Wizard SV Gel and PCR Clean-Up System (Promega Biosciences, San Luis Obispo, CA), cloned into a pENTR plasmid vector with the Directional TOPO Cloning Kit (Invitrogen), and completely sequenced using BigDye Terminator kit (PE Applied Biosystems, Foster City, CA) and DyeEX 96 Kit (Qiagen Valencia, CA) on ABI 3100 Genetic Analyzer (PE Applied Biosystems). .. The sequence was submitted to Genbank (accession number: ).

    Article Title: Massive Expansion of Gypsy-Like Retrotransposons in Microbotryum Fungi
    Article Snippet: The thermal profile consisted of initial denaturation at 95°C for 3 min; 30 cycles of amplification with each comprising 95°C for 30 s, touchdown from 64°C to 59°C with a 1°C decrease after every six cycles for 30 s, and 68°C for 1 min; and final extension at 68°C for 7 min. We cloned the resulting products using a TOPO TA cloning kit (Invitrogen), then re-amplified forty clones from each chromosome using the PCR protocol described earlier, except with the initial denaturation step increased to 10 min. We purified the PCR products using ExoSAP-IT (Affymetrix). .. Clones were sequenced using the BigDye Terminator kit on an ABI Prism 377XL sequencer (Applied Biosystems) at the University of Massachusetts Amherst DNA sequencing facility.

    Article Title: Geographical Gradient of the eIF4E Alleles Conferring Resistance to Potyviruses in Pea (Pisum) Germplasm
    Article Snippet: .. The PCR products were purified according to Werle et al. protocol and sequenced using a BigDye Terminator kit (Applied Biosystems, UK) by Macrogene (Amsterdam, The Netherlands). .. Bioinformatics Primer design and restriction analyses were performed using FastPCR software version 5.1.83 (PrimerDigital Ltd., Finland).

    Article Title: The Murine Norovirus Core Subgenomic RNA Promoter Consists of a Stable Stem-Loop That Can Direct Accurate Initiation of RNA Synthesis
    Article Snippet: Purified cDNAs were first subjected to the 3′-end poly(C) tailing reaction catalyzed by terminal transferase, followed by PCR using an anchor primer that anneals to the poly(C) 3′ end of cDNA and the MNV-specific reverse primer. .. Sequences of the PCR products were determined using the BigDye Terminator kit and analyzed using the Vector NTI software (Invitrogen).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Epidemiology and Clinical Associations of Human Parechovirus Respiratory Infections ▿
    Article Snippet: Each combined RT-PCR was carried out in a 20-μl reaction volume of the manufacturers' supplied buffer (containing 0.4 mM deoxynucleoside triphosphates and 2.4 mM MgSO4 ), 6 μl of extracted RNA, 0.8 μl of Superscript III RT/Platinum Taq polymerase, and 0.5 μM of outer VP3/VP1 primers. .. Amplified DNA was directly sequenced using a BigDye Terminator kit (Applied Biosystems, Warrington, United Kingdom) in both directions, from which a complete sequence between positions 2182 and 2437 was assembled.

    Nested PCR:

    Article Title: Molecular Epidemiology and Spatial Distribution of a Waterborne Cryptosporidiosis Outbreak in Australia ▿
    Article Snippet: A previously described nested PCR targeting the hypervariable gp60 gene was performed to identify Cryptosporidium subtype families and subtypes ( ). .. Reaction mixtures containing the correct size fragment (∼1,000 bp) were purified as described above and sequenced using an ABI 3130xl genetic analyzer (Applied Biosystems, Foster City, CA) with a BigDye Terminator kit (Applied Biosystems).

    Rapid Amplification of cDNA Ends:

    Article Title: Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey
    Article Snippet: To determine the 5′ and 3′ ends of the cDNAs, the rapid amplification of cDNA ends (RACE) technique was carried out using the SMART RACE cDNA amplification kit (Clontech, Palo Alto, CA, USA) with gene-specific primers designed on the basis of the internal fragment sequences described previously ( ). .. The nucleotide sequence was determined using a BigDye Terminator kit (Thermo Fisher Scientific, Waltham, MA, USA) and an ABI PRISM 3130 genetic analyzer (Thermo Fisher Scientific).

    Article Title: The Murine Norovirus Core Subgenomic RNA Promoter Consists of a Stable Stem-Loop That Can Direct Accurate Initiation of RNA Synthesis
    Article Snippet: The purified cDNAs were then used as a template for rapid amplification of cDNA ends (RACE) assay using 5′ RACE according to the manufacturer's instructions (Life Technologies). .. Sequences of the PCR products were determined using the BigDye Terminator kit and analyzed using the Vector NTI software (Invitrogen).

    Plasmid Preparation:

    Article Title: Zscan4: a novel gene expressed exclusively in late 2-cell embryos and embryonic stem cells
    Article Snippet: .. A full-length cDNA clone was PCR-amplified with Ex Taq Polymerase (Takara Mirus Bio, Madison, WI), purified with the Wizard SV Gel and PCR Clean-Up System (Promega Biosciences, San Luis Obispo, CA), cloned into a pENTR plasmid vector with the Directional TOPO Cloning Kit (Invitrogen), and completely sequenced using BigDye Terminator kit (PE Applied Biosystems, Foster City, CA) and DyeEX 96 Kit (Qiagen Valencia, CA) on ABI 3100 Genetic Analyzer (PE Applied Biosystems). .. The sequence was submitted to Genbank (accession number: ).

    Article Title: Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract
    Article Snippet: The 16S rRNA gene from each of the ASF strains was amplified using bacterium-specific 16S primers 27F and 1492R ( ) and cloned into a pCR2.1 plasmid vector using the Topo-TA cloning kit (Invitrogen). .. The plasmids were sequenced using the BigDye terminator kit (Applied Biosystems) to ensure that the primer regions were not altered due to Taq polymerase error.

    Article Title: The Murine Norovirus Core Subgenomic RNA Promoter Consists of a Stable Stem-Loop That Can Direct Accurate Initiation of RNA Synthesis
    Article Snippet: .. Sequences of the PCR products were determined using the BigDye Terminator kit and analyzed using the Vector NTI software (Invitrogen). .. Primer extension analysis was performed on RNA isolated from either infected cells or cells transfected with in vitro -transcribed capped RNAs generated from MNV cDNA-containing clones.

    Software:

    Article Title: The Murine Norovirus Core Subgenomic RNA Promoter Consists of a Stable Stem-Loop That Can Direct Accurate Initiation of RNA Synthesis
    Article Snippet: .. Sequences of the PCR products were determined using the BigDye Terminator kit and analyzed using the Vector NTI software (Invitrogen). .. Primer extension analysis was performed on RNA isolated from either infected cells or cells transfected with in vitro -transcribed capped RNAs generated from MNV cDNA-containing clones.

    Real-time Polymerase Chain Reaction:

    Article Title: Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract
    Article Snippet: Paragraph title: QPCR standards. ... The plasmids were sequenced using the BigDye terminator kit (Applied Biosystems) to ensure that the primer regions were not altered due to Taq polymerase error.

    Agarose Gel Electrophoresis:

    Article Title: Compound Heterozygosity of the Functionally NullCdh23v-ngt and HypomorphicCdh23ahl Alleles Leads to Early-onset Progressive Hearing Loss in Mice
    Article Snippet: PCR amplification was carried out using a KAPA2G Fast PCR Kit (Kapa Biosystems) and primer set A (Supplementary Table 1: refer to J-STAGE at https://www.jstage.jst.go.jp/browse/expanim) and consisted of 40 cycles at 95°C for 20 s, 60°C for 20 s and 72°C for 5 s; the products were digested with Bst NI (New England BioLabs, Ipswich, MA, USA) at 65°C for 1 h and then subjected to 4% agarose gel electrophoresis. .. The Cdh23ahl mutation was confirmed by DNA sequencing of the products amplified by primer set B (Supplementary Table 1) using a BigDye Terminator kit (Life Technologies, Grand Island, NY, USA) and an Applied Biosystems 3130xl Genetic Analyzer.

    Article Title: Molecular Epidemiology and Spatial Distribution of a Waterborne Cryptosporidiosis Outbreak in Australia ▿
    Article Snippet: The amplicons were separated on 2% (wt/vol) agarose gel containing Sybr safe and were visualized under UV light. .. Reaction mixtures containing the correct size fragment (∼1,000 bp) were purified as described above and sequenced using an ABI 3130xl genetic analyzer (Applied Biosystems, Foster City, CA) with a BigDye Terminator kit (Applied Biosystems).

    Staining:

    Article Title: Development of specific primers for Fusarium oxysporum causing damping off of Lilium formolongi
    Article Snippet: The agarose gels were stained with ethidium bromide (10 mg/ml) and visualized under UV transillumination. .. Amplified products from lily isolates were selected when the size of fragments was distinguished from other isolates, and used for sequencing reactions using a BigDye Terminator kit and a 3700XL Genetic Analyzer (Applied Biosystems).

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    Thermo Fisher bigdye terminater v3 1 cycle sequencing kit
    Bigdye Terminater V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    bigdye terminater v3 1 cycle sequencing kit - by Bioz Stars, 2020-02
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