bigdye terminator cycle sequencing v3 1 kits  (Thermo Fisher)


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    Name:
    BigDye Terminator v3 1 Cycle Sequencing Kit
    Description:
    The BigDye Terminator v3 1 Cycle Sequencing Kit s robust highly flexible chemistry is ideal for de novo sequencing resequencing and finishing with PCR product Plasmid Fosmid and BAC templates • Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT rich regions • Get longer higher quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs Get More Uniform Peak Heights Improved AccuracyWith BigDye Terminator v3 1 kit superior chemistry you generate data with uniform peak heights and optimized signal balance This results in longer higher quality reads and more accurate base assignments for heterozygote and mutation detection For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4337454
    Price:
    None
    Applications:
    Kits & Reagents for Sanger Sequencing|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Sequencing
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher bigdye terminator cycle sequencing v3 1 kits
    The BigDye Terminator v3 1 Cycle Sequencing Kit s robust highly flexible chemistry is ideal for de novo sequencing resequencing and finishing with PCR product Plasmid Fosmid and BAC templates • Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT rich regions • Get longer higher quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs Get More Uniform Peak Heights Improved AccuracyWith BigDye Terminator v3 1 kit superior chemistry you generate data with uniform peak heights and optimized signal balance This results in longer higher quality reads and more accurate base assignments for heterozygote and mutation detection For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/bigdye terminator cycle sequencing v3 1 kits/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator cycle sequencing v3 1 kits - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A major gene controls mimicry and crypsis in butterflies and moths
    Article Snippet: Cloning of products was performed using TOPO TA (Invitrogen) or pGEM-T (Promega) cloning kits. .. Sanger sequencing was performed using BigDye terminator v3.1 (Applied Biosystems) run on an ABI13730 capillary sequencer.

    Amplification:

    Article Title: Fungal endophytes in above-ground tissues of desert plants: infrequent in culture, but highly diverse and distinctive symbionts
    Article Snippet: When amplification with these primers failed, we amplified only ITSrDNA using primers ITS1F or ITS5 and ITS4 [ ]. .. PCR products were verified by gel electrophoresis, cleaned using ExoSap-IT (Affymetrix; Santa Clara, CA, USA), and sequenced bidirectionally using the Applied Biosystems BigDye Terminator v 3.1 cycle sequencing kit and the original PCR primers on an Applied Biosystems 3730 xl DNA Analyzer (Foster City, CA, USA) at the University of Arizona Genetics Core.

    Article Title: Validation and Comparison of Pharmacogenetics-Based Warfarin Dosing Algorithms for Application of Pharmacogenetic Testing
    Article Snippet: .. Specifically, amplified DNA products were purified with a PCR Cleanup kit (Promega Corp., Madison, WI) and analyzed with the BigDye Terminator v3.1 Cycler Sequencing Kit (Applied Biosystems, Foster City, CA) before loading onto the ABI 3130XL. ..

    Article Title: A network of conserved co-occurring motifs for the regulation of alternative splicing
    Article Snippet: Paragraph title: Reverse transcription polymerase chain reaction and amplification of splicing products ... After isolation of the PCR products on agarose gel, DNA sequencing of the PCR products was done on an ABI 3130 (Applied Biosystems, Foster City, CA) using a Big Dye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems).

    Article Title: A major gene controls mimicry and crypsis in butterflies and moths
    Article Snippet: The gene specific primer used for the first round of amplification was anchored in exon 4 (fzl_raceex5_R1, ). .. Sanger sequencing was performed using BigDye terminator v3.1 (Applied Biosystems) run on an ABI13730 capillary sequencer.

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: Paragraph title: DNA Extraction, PCR Amplification, and Sequencing ... Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    Article Title: Molecular epidemiology of salmonid alphavirus (SAV) subtype 3 in Norway
    Article Snippet: Briefly, extracted RNA was reverse transcribed using random primers and SuperScript III RT (Invitrogen) or OneStep (Qiagen); 2.5 μl cDNA with 0.15 μM of each primer were added in a final PCR reaction volume of 25 μl (HotStar Taq PCR; Qiagen) under the following conditions: denaturation for 15 minutes at 95°C, followed by 40 amplification cycles of 94°C 30 sec, 59°C 30 sec and 72°C 90 sec, and finally 72°C for 10 min. .. The RT-PCR products were examined by agar gel electrophoresis and purified using the ExoSAP-IT protocol (Usb) prior to sequencing with BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems).

    Article Title: Genotypic and PFGE/MLVA Analyses of Vibrio cholerae O1: Geographical Spread and Temporal Changes during the 2007-2010 Cholera Outbreaks in Thailand
    Article Snippet: These loci were amplified using specific primers and could be identified by those genes in the order of occurrence: VC0147, VC0436-7, VC1650, VC0171, and VCA0283. .. PCR products were purified using the NucleoSpin Extract II Kit (Macherey-Nagel), sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and loaded onto an ABI 3130xl automated sequencer (Applied Biosystems) according to the manufacturer's instructions.

    Article Title: The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
    Article Snippet: In cases of poor amplification primer pair Tox1F_Coding and a new conserved reverse primer, Tox1R_Conserved , were used to confirm observed sequence variation. .. Sequencing reactions were conducted in 10 µl volume using the BigDye Terminator v3.1 Sequencing Standard Kit (Applied Biosystems) with both the forward and the reverse primer.

    Article Title: Association of polymorphisms of the TNFRSF11B and TNFSF11 genes with bone mineral density in postmenopausal women from western Mexico
    Article Snippet: Identification of polymorphisms was performed by PCR followed by Sanger DNA sequencing with the BigDye terminator kit v3.1 (Applied Biosystems Foster City). .. To identify the rs2073618 and rs3102734 variants, a fragment of 274 bp was amplified using the primers forward 5´-GATCAAAGGCAGGCGATACTTCC- 3´ and reverse 5´-CTGGGAGGGAGCGAGTGGAGC- 3´.

    Article Title: Homozygous Triplicate Mutations in Three 16S rRNA Genes Responsible for High-Level Aminoglycoside Resistance in Nocardia farcinica Clinical Isolates from a Canada-Wide Bovine Mastitis Epizootic ▿
    Article Snippet: Genomic 16S rRNA genes rrnA , rrnB , and rrnC of N. farcinica strains ( ) were amplified by using the following forward primers rrnA-Fw (5′-TACCCGAACTTCGTCGAG-3′), rrnB-Fw (5′-ACACCTCGCAATCGGGTACG-3′), and rrnC-Fw (5′-GTGAACAACCAGAAGATCGC-3′), respectively (Fig. ). .. For nucleotide sequencing, BigDye Terminator v3.1 cycle sequencing kits and a 3130 Genetic Analyzer (Applied Biosystems) were used in accordance with the manufacturer's instructions.

    Quantitative RT-PCR:

    Article Title: A major gene controls mimicry and crypsis in butterflies and moths
    Article Snippet: Paragraph title: 5’ RACE, RT-PCR and qRT-PCR ... Sanger sequencing was performed using BigDye terminator v3.1 (Applied Biosystems) run on an ABI13730 capillary sequencer.

    SYBR Green Assay:

    Article Title: A major gene controls mimicry and crypsis in butterflies and moths
    Article Snippet: Sanger sequencing was performed using BigDye terminator v3.1 (Applied Biosystems) run on an ABI13730 capillary sequencer. .. We performed qRT-PCR using SensiMix SYBR green (Bioline Reagents Ltd.) with 0.2-0.25µM of each primer and 1μl of the diluted product from the cDNA reactions.

    Incubation:

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
    Article Snippet: The reaction mixture was incubated at 37°C for 12 h in the presence of 30% ethylene glycol and 5% glycerol to accumulate the cleavage products ( ). .. DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific).

    Expressing:

    Article Title: A major gene controls mimicry and crypsis in butterflies and moths
    Article Snippet: Sanger sequencing was performed using BigDye terminator v3.1 (Applied Biosystems) run on an ABI13730 capillary sequencer. .. Primers fzl_ex1a_F1 and fzl_ex4_R1 were used to confirm expression of the furthest 5’ UTR.

    Sequencing:

    Article Title: Familial STAG2 germline mutation defines a new human cohesinopathy
    Article Snippet: .. Sanger sequencing was achieved by standard methods using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer. .. Sequencing data was analyzed using the software Sequencher version 4.1.4 (Gene Code).

    Article Title: Fungal endophytes in above-ground tissues of desert plants: infrequent in culture, but highly diverse and distinctive symbionts
    Article Snippet: .. PCR products were verified by gel electrophoresis, cleaned using ExoSap-IT (Affymetrix; Santa Clara, CA, USA), and sequenced bidirectionally using the Applied Biosystems BigDye Terminator v 3.1 cycle sequencing kit and the original PCR primers on an Applied Biosystems 3730 xl DNA Analyzer (Foster City, CA, USA) at the University of Arizona Genetics Core. .. DNA sequences were assembled in phred [ ] and phrap [ ] with orchestration by Mesquite v. 1.06 [ ] and then manually verified and edited in Sequencher v. 5.1 (Gene Codes Corporation, Ann Arbor, MI, USA).

    Article Title: Validation and Comparison of Pharmacogenetics-Based Warfarin Dosing Algorithms for Application of Pharmacogenetic Testing
    Article Snippet: .. Specifically, amplified DNA products were purified with a PCR Cleanup kit (Promega Corp., Madison, WI) and analyzed with the BigDye Terminator v3.1 Cycler Sequencing Kit (Applied Biosystems, Foster City, CA) before loading onto the ABI 3130XL. ..

    Article Title: A network of conserved co-occurring motifs for the regulation of alternative splicing
    Article Snippet: .. After isolation of the PCR products on agarose gel, DNA sequencing of the PCR products was done on an ABI 3130 (Applied Biosystems, Foster City, CA) using a Big Dye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems). .. Identification of intronic splicing regulatory motifs by using genome alignments To systematically identify and predict sequence motifs that point to the regulation of alternative splicing, we focused on alternatively skipped exons as they are straightforward to measure and are the most frequent type of alternative splicing ( ).

    Article Title: A major gene controls mimicry and crypsis in butterflies and moths
    Article Snippet: .. Sanger sequencing was performed using BigDye terminator v3.1 (Applied Biosystems) run on an ABI13730 capillary sequencer. .. Primers fzl_ex1a_F1 and fzl_ex4_R1 were used to confirm expression of the furthest 5’ UTR.

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: .. Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA). ..

    Article Title: Molecular epidemiology of salmonid alphavirus (SAV) subtype 3 in Norway
    Article Snippet: .. The RT-PCR products were examined by agar gel electrophoresis and purified using the ExoSAP-IT protocol (Usb) prior to sequencing with BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems). .. Sequence analysis Consensus sequences were generated using Sequencher (Gene Codes Corporation) or ChromasPro (Technelysium Pty Ltd).

    Article Title: Genotypic and PFGE/MLVA Analyses of Vibrio cholerae O1: Geographical Spread and Temporal Changes during the 2007-2010 Cholera Outbreaks in Thailand
    Article Snippet: .. PCR products were purified using the NucleoSpin Extract II Kit (Macherey-Nagel), sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and loaded onto an ABI 3130xl automated sequencer (Applied Biosystems) according to the manufacturer's instructions. .. Sequence data for each isolate were read using BioEdit Sequence Alignment Editor v.7.0.5.3 ( http://www.mbio.ncsu.edu/bioedit/bioedit.html ) .

    Article Title: The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
    Article Snippet: .. Sequencing reactions were conducted in 10 µl volume using the BigDye Terminator v3.1 Sequencing Standard Kit (Applied Biosystems) with both the forward and the reverse primer. ..

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
    Article Snippet: .. DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific). .. Electrophoresis mobility shift assays DNA fragments containing the att sites were amplified with the primer sets P110/P61 (for attP ; 89 bp), P111/P87 (for attB ; 106 bp), P111/P58 (for attL ; 111 bp), and P7/P87 (for attR ; 132 bp).

    Article Title: Homozygous Triplicate Mutations in Three 16S rRNA Genes Responsible for High-Level Aminoglycoside Resistance in Nocardia farcinica Clinical Isolates from a Canada-Wide Bovine Mastitis Epizootic ▿
    Article Snippet: .. For nucleotide sequencing, BigDye Terminator v3.1 cycle sequencing kits and a 3130 Genetic Analyzer (Applied Biosystems) were used in accordance with the manufacturer's instructions. .. The 16S rRNA sequence of IFM 10580 (W6220) was deposited to GenBank (GenBank/EMBL/DDBJ accession no. ) ( ).

    Southern Blot:

    Article Title: The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
    Article Snippet: The entire SnTox1 coding region was PCR amplified from the genomic DNA of SN15 and used as a probe for Southern blot analysis. .. Sequencing reactions were conducted in 10 µl volume using the BigDye Terminator v3.1 Sequencing Standard Kit (Applied Biosystems) with both the forward and the reverse primer.

    DNA Sequencing:

    Article Title: A network of conserved co-occurring motifs for the regulation of alternative splicing
    Article Snippet: .. After isolation of the PCR products on agarose gel, DNA sequencing of the PCR products was done on an ABI 3130 (Applied Biosystems, Foster City, CA) using a Big Dye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems). .. Identification of intronic splicing regulatory motifs by using genome alignments To systematically identify and predict sequence motifs that point to the regulation of alternative splicing, we focused on alternatively skipped exons as they are straightforward to measure and are the most frequent type of alternative splicing ( ).

    Article Title: Association of polymorphisms of the TNFRSF11B and TNFSF11 genes with bone mineral density in postmenopausal women from western Mexico
    Article Snippet: .. Identification of polymorphisms was performed by PCR followed by Sanger DNA sequencing with the BigDye terminator kit v3.1 (Applied Biosystems Foster City). .. To identify the rs2073618 and rs3102734 variants, a fragment of 274 bp was amplified using the primers forward 5´-GATCAAAGGCAGGCGATACTTCC- 3´ and reverse 5´-CTGGGAGGGAGCGAGTGGAGC- 3´.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A network of conserved co-occurring motifs for the regulation of alternative splicing
    Article Snippet: Paragraph title: Reverse transcription polymerase chain reaction and amplification of splicing products ... After isolation of the PCR products on agarose gel, DNA sequencing of the PCR products was done on an ABI 3130 (Applied Biosystems, Foster City, CA) using a Big Dye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems).

    Article Title: A major gene controls mimicry and crypsis in butterflies and moths
    Article Snippet: Paragraph title: 5’ RACE, RT-PCR and qRT-PCR ... Sanger sequencing was performed using BigDye terminator v3.1 (Applied Biosystems) run on an ABI13730 capillary sequencer.

    Article Title: Molecular epidemiology of salmonid alphavirus (SAV) subtype 3 in Norway
    Article Snippet: .. The RT-PCR products were examined by agar gel electrophoresis and purified using the ExoSAP-IT protocol (Usb) prior to sequencing with BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems). .. Sequence analysis Consensus sequences were generated using Sequencher (Gene Codes Corporation) or ChromasPro (Technelysium Pty Ltd).

    DNA Extraction:

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: Paragraph title: DNA Extraction, PCR Amplification, and Sequencing ... Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Fungal endophytes in above-ground tissues of desert plants: infrequent in culture, but highly diverse and distinctive symbionts
    Article Snippet: .. PCR products were verified by gel electrophoresis, cleaned using ExoSap-IT (Affymetrix; Santa Clara, CA, USA), and sequenced bidirectionally using the Applied Biosystems BigDye Terminator v 3.1 cycle sequencing kit and the original PCR primers on an Applied Biosystems 3730 xl DNA Analyzer (Foster City, CA, USA) at the University of Arizona Genetics Core. .. DNA sequences were assembled in phred [ ] and phrap [ ] with orchestration by Mesquite v. 1.06 [ ] and then manually verified and edited in Sequencher v. 5.1 (Gene Codes Corporation, Ann Arbor, MI, USA).

    Article Title: Molecular epidemiology of salmonid alphavirus (SAV) subtype 3 in Norway
    Article Snippet: .. The RT-PCR products were examined by agar gel electrophoresis and purified using the ExoSAP-IT protocol (Usb) prior to sequencing with BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems). .. Sequence analysis Consensus sequences were generated using Sequencher (Gene Codes Corporation) or ChromasPro (Technelysium Pty Ltd).

    Mutagenesis:

    Article Title: Familial STAG2 germline mutation defines a new human cohesinopathy
    Article Snippet: Allele-specific PCR was achieved by synthesizing long primers that differed on their 3′ extremity, where the mutation was located. .. Sanger sequencing was achieved by standard methods using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer.

    Isolation:

    Article Title: A network of conserved co-occurring motifs for the regulation of alternative splicing
    Article Snippet: .. After isolation of the PCR products on agarose gel, DNA sequencing of the PCR products was done on an ABI 3130 (Applied Biosystems, Foster City, CA) using a Big Dye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems). .. Identification of intronic splicing regulatory motifs by using genome alignments To systematically identify and predict sequence motifs that point to the regulation of alternative splicing, we focused on alternatively skipped exons as they are straightforward to measure and are the most frequent type of alternative splicing ( ).

    Size-exclusion Chromatography:

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: The amplification conditions consisted of an initial denaturation at 94°C for 2 min; followed by 35 cycles of 94°C for 30 s, 60°C for 30 s, and 72°C for 30 sec; followed by a final extension step at 72°C for 2 min. Each 20-µl reaction mixture consisted of 14.8 µl of water, 2 µl of 10× PCR Buffer (Bioneer Co., Korea), 1.6 µl of dNTPs (10 mM), 0.2 µl of each primer (1.6 µM), 0.1 µl of Top DNA polymerase (0.5 U/µM) (Bioneer Co., Korea), and 1 µl of sample DNA. .. Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    Article Title: Molecular epidemiology of salmonid alphavirus (SAV) subtype 3 in Norway
    Article Snippet: Briefly, extracted RNA was reverse transcribed using random primers and SuperScript III RT (Invitrogen) or OneStep (Qiagen); 2.5 μl cDNA with 0.15 μM of each primer were added in a final PCR reaction volume of 25 μl (HotStar Taq PCR; Qiagen) under the following conditions: denaturation for 15 minutes at 95°C, followed by 40 amplification cycles of 94°C 30 sec, 59°C 30 sec and 72°C 90 sec, and finally 72°C for 10 min. .. The RT-PCR products were examined by agar gel electrophoresis and purified using the ExoSAP-IT protocol (Usb) prior to sequencing with BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems).

    Purification:

    Article Title: Validation and Comparison of Pharmacogenetics-Based Warfarin Dosing Algorithms for Application of Pharmacogenetic Testing
    Article Snippet: .. Specifically, amplified DNA products were purified with a PCR Cleanup kit (Promega Corp., Madison, WI) and analyzed with the BigDye Terminator v3.1 Cycler Sequencing Kit (Applied Biosystems, Foster City, CA) before loading onto the ABI 3130XL. ..

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: The PCR products were purified by Exosap-IT (GE Healthcare, England). .. Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    Article Title: Molecular epidemiology of salmonid alphavirus (SAV) subtype 3 in Norway
    Article Snippet: .. The RT-PCR products were examined by agar gel electrophoresis and purified using the ExoSAP-IT protocol (Usb) prior to sequencing with BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems). .. Sequence analysis Consensus sequences were generated using Sequencher (Gene Codes Corporation) or ChromasPro (Technelysium Pty Ltd).

    Article Title: Genotypic and PFGE/MLVA Analyses of Vibrio cholerae O1: Geographical Spread and Temporal Changes during the 2007-2010 Cholera Outbreaks in Thailand
    Article Snippet: .. PCR products were purified using the NucleoSpin Extract II Kit (Macherey-Nagel), sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and loaded onto an ABI 3130xl automated sequencer (Applied Biosystems) according to the manufacturer's instructions. .. Sequence data for each isolate were read using BioEdit Sequence Alignment Editor v.7.0.5.3 ( http://www.mbio.ncsu.edu/bioedit/bioedit.html ) .

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
    Article Snippet: .. DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific). .. Electrophoresis mobility shift assays DNA fragments containing the att sites were amplified with the primer sets P110/P61 (for attP ; 89 bp), P111/P87 (for attB ; 106 bp), P111/P58 (for attL ; 111 bp), and P7/P87 (for attR ; 132 bp).

    Polymerase Chain Reaction:

    Article Title: Familial STAG2 germline mutation defines a new human cohesinopathy
    Article Snippet: Paragraph title: Allele-specific PCR and Sanger sequencing ... Sanger sequencing was achieved by standard methods using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer.

    Article Title: Fungal endophytes in above-ground tissues of desert plants: infrequent in culture, but highly diverse and distinctive symbionts
    Article Snippet: .. PCR products were verified by gel electrophoresis, cleaned using ExoSap-IT (Affymetrix; Santa Clara, CA, USA), and sequenced bidirectionally using the Applied Biosystems BigDye Terminator v 3.1 cycle sequencing kit and the original PCR primers on an Applied Biosystems 3730 xl DNA Analyzer (Foster City, CA, USA) at the University of Arizona Genetics Core. .. DNA sequences were assembled in phred [ ] and phrap [ ] with orchestration by Mesquite v. 1.06 [ ] and then manually verified and edited in Sequencher v. 5.1 (Gene Codes Corporation, Ann Arbor, MI, USA).

    Article Title: Validation and Comparison of Pharmacogenetics-Based Warfarin Dosing Algorithms for Application of Pharmacogenetic Testing
    Article Snippet: .. Specifically, amplified DNA products were purified with a PCR Cleanup kit (Promega Corp., Madison, WI) and analyzed with the BigDye Terminator v3.1 Cycler Sequencing Kit (Applied Biosystems, Foster City, CA) before loading onto the ABI 3130XL. ..

    Article Title: A network of conserved co-occurring motifs for the regulation of alternative splicing
    Article Snippet: .. After isolation of the PCR products on agarose gel, DNA sequencing of the PCR products was done on an ABI 3130 (Applied Biosystems, Foster City, CA) using a Big Dye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems). .. Identification of intronic splicing regulatory motifs by using genome alignments To systematically identify and predict sequence motifs that point to the regulation of alternative splicing, we focused on alternatively skipped exons as they are straightforward to measure and are the most frequent type of alternative splicing ( ).

    Article Title: A major gene controls mimicry and crypsis in butterflies and moths
    Article Snippet: Secondary PCR of these products was then performed using a primer in exon 2 (HM25_long_F2, ) and the nested universal primer A. .. Sanger sequencing was performed using BigDye terminator v3.1 (Applied Biosystems) run on an ABI13730 capillary sequencer.

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: Paragraph title: DNA Extraction, PCR Amplification, and Sequencing ... Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    Article Title: Molecular epidemiology of salmonid alphavirus (SAV) subtype 3 in Norway
    Article Snippet: Briefly, extracted RNA was reverse transcribed using random primers and SuperScript III RT (Invitrogen) or OneStep (Qiagen); 2.5 μl cDNA with 0.15 μM of each primer were added in a final PCR reaction volume of 25 μl (HotStar Taq PCR; Qiagen) under the following conditions: denaturation for 15 minutes at 95°C, followed by 40 amplification cycles of 94°C 30 sec, 59°C 30 sec and 72°C 90 sec, and finally 72°C for 10 min. .. The RT-PCR products were examined by agar gel electrophoresis and purified using the ExoSAP-IT protocol (Usb) prior to sequencing with BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems).

    Article Title: Genotypic and PFGE/MLVA Analyses of Vibrio cholerae O1: Geographical Spread and Temporal Changes during the 2007-2010 Cholera Outbreaks in Thailand
    Article Snippet: .. PCR products were purified using the NucleoSpin Extract II Kit (Macherey-Nagel), sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) and loaded onto an ABI 3130xl automated sequencer (Applied Biosystems) according to the manufacturer's instructions. .. Sequence data for each isolate were read using BioEdit Sequence Alignment Editor v.7.0.5.3 ( http://www.mbio.ncsu.edu/bioedit/bioedit.html ) .

    Article Title: The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
    Article Snippet: The entire SnTox1 coding region was PCR amplified from the genomic DNA of SN15 and used as a probe for Southern blot analysis. .. Sequencing reactions were conducted in 10 µl volume using the BigDye Terminator v3.1 Sequencing Standard Kit (Applied Biosystems) with both the forward and the reverse primer.

    Article Title: Association of polymorphisms of the TNFRSF11B and TNFSF11 genes with bone mineral density in postmenopausal women from western Mexico
    Article Snippet: .. Identification of polymorphisms was performed by PCR followed by Sanger DNA sequencing with the BigDye terminator kit v3.1 (Applied Biosystems Foster City). .. To identify the rs2073618 and rs3102734 variants, a fragment of 274 bp was amplified using the primers forward 5´-GATCAAAGGCAGGCGATACTTCC- 3´ and reverse 5´-CTGGGAGGGAGCGAGTGGAGC- 3´.

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
    Article Snippet: Two microgram of the PCR product were mixed with 0.3 μM SprA in 100 μl of the reaction solution containing 10 mM Tris–HCl (pH 8.0), 20 mM NaCl and 0.1 mM DTT. .. DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific).

    Lysis:

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: Genomic DNA was extracted using sodium hydroxide (NaOH) direct lysis protocol . .. Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    Software:

    Article Title: Familial STAG2 germline mutation defines a new human cohesinopathy
    Article Snippet: Sanger sequencing was achieved by standard methods using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer. .. Sequencing data was analyzed using the software Sequencher version 4.1.4 (Gene Code).

    Article Title: The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
    Article Snippet: Sequencing reactions were conducted in 10 µl volume using the BigDye Terminator v3.1 Sequencing Standard Kit (Applied Biosystems) with both the forward and the reverse primer. .. Alignment of forward and reverse sequences for each isolate was performed in SeqScape software V2.5 (Applied Biosystems, Foster City, CA).

    DNA Hybridization:

    Article Title: The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
    Article Snippet: Probe preparation, DNA hybridization, membrane washing and image acquisition followed the protocol described by Faris et al. . .. Sequencing reactions were conducted in 10 µl volume using the BigDye Terminator v3.1 Sequencing Standard Kit (Applied Biosystems) with both the forward and the reverse primer.

    Electrophoresis:

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: The PCR reactions were analyzed by electrophoresis on a 1.5% agarose gel and visualized by UV illumination by ethidium bromide staining. .. Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    RNA Extraction:

    Article Title: Molecular epidemiology of salmonid alphavirus (SAV) subtype 3 in Norway
    Article Snippet: Paragraph title: RNA extraction and Rt RT-PCR ... The RT-PCR products were examined by agar gel electrophoresis and purified using the ExoSAP-IT protocol (Usb) prior to sequencing with BigDye® Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems).

    Selection:

    Article Title: Validation and Comparison of Pharmacogenetics-Based Warfarin Dosing Algorithms for Application of Pharmacogenetic Testing
    Article Snippet: A random selection of 10 samples was subjected to confirmatory automated sequencing using an ABI3130XL (Applied Biosystems, Foster City, CA). .. Specifically, amplified DNA products were purified with a PCR Cleanup kit (Promega Corp., Madison, WI) and analyzed with the BigDye Terminator v3.1 Cycler Sequencing Kit (Applied Biosystems, Foster City, CA) before loading onto the ABI 3130XL.

    Agarose Gel Electrophoresis:

    Article Title: A network of conserved co-occurring motifs for the regulation of alternative splicing
    Article Snippet: .. After isolation of the PCR products on agarose gel, DNA sequencing of the PCR products was done on an ABI 3130 (Applied Biosystems, Foster City, CA) using a Big Dye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems). .. Identification of intronic splicing regulatory motifs by using genome alignments To systematically identify and predict sequence motifs that point to the regulation of alternative splicing, we focused on alternatively skipped exons as they are straightforward to measure and are the most frequent type of alternative splicing ( ).

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: The PCR reactions were analyzed by electrophoresis on a 1.5% agarose gel and visualized by UV illumination by ethidium bromide staining. .. Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    Staining:

    Article Title: A network of conserved co-occurring motifs for the regulation of alternative splicing
    Article Snippet: The PCR products were resolved on 4% NuSeive GTG agarose gel (Takara Bio Inc.) and then detected by fluorescent staining with ethidium bromide. .. After isolation of the PCR products on agarose gel, DNA sequencing of the PCR products was done on an ABI 3130 (Applied Biosystems, Foster City, CA) using a Big Dye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems).

    Article Title: First Report of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) Infections in Invasive Rodents from Five Islands of the Ogasawara Archipelago, Japan
    Article Snippet: The PCR reactions were analyzed by electrophoresis on a 1.5% agarose gel and visualized by UV illumination by ethidium bromide staining. .. Sequencing was performed using the BigDye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, USA) according to the manufacturer’s instruction, and determined by ABI3100 automatic sequencer (Applied Biosystems, USA).

    Variant Assay:

    Article Title: Familial STAG2 germline mutation defines a new human cohesinopathy
    Article Snippet: Allele-specific PCR and Sanger sequencing The presence of the c.980 G > A variant in STAG2 gene in the proband and his relatives was confirmed using allele-specific PCR and Sanger sequencing. .. Sanger sequencing was achieved by standard methods using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer.

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    Thermo Fisher bigdye terminater v3 1 cycle sequencing kit
    Bigdye Terminater V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminater v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bigdye terminater v3 1 cycle sequencing kit - by Bioz Stars, 2020-04
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